FastQCFastQC Report
Fri 17 Jun 2016
SRR1526066_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526066_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44360
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG14183.196573489630298No Hit
TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG3530.7957619477006312No Hit
TGTGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT3110.7010820559062219No Hit
TGTGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC2890.651487826871055No Hit
TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1980.44634806131650134No Hit
TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG1620.3651938683498647No Hit
TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG1170.26375112714156895No Hit
TGTGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC950.21415689810640215No Hit
TGTGCGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA790.17808836789900812No Hit
TGTGCGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT780.17583408476104598No Hit
TGTGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG650.14652840396753833No Hit
TGTGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT640.1442741208295762No Hit
TGTGCGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA620.13976555455365194No Hit
TGTGCGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC600.1352569882777277No Hit
TGTGCGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC560.12623985572587917No Hit
TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC550.12398557258791704No Hit
TGTGCGGGGGTCCCTAGCCCACTCCCTGTGTTTCCTTTCTCTCTGGAATGGAGGGA510.11496844003606854No Hit
TGTGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA490.11045987376014427No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.1036970243462579No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGGA150.00802486350.5463941
TCAATCT150.00802486350.5463941
CGTGCAT150.00802486350.5463941
GCGTGCA203.945602E-450.48855640
GATGCGT203.990456E-450.37328337
TAGCCCA150.00820838850.2585415
TGGGGAT150.00820838850.2585415
GACCGTG150.00820838850.2585445
ATTCGTG150.00820838850.2585414
GTAAGAA150.00820838850.2585446
CTAGCAA150.00820838850.2585446
TTAAAGA150.00820838850.2585445
GGATGCG252.0062987E-550.20136636
TATGCTC550.050.20136635
TCTGGAA150.00824546550.20136642
CACGATT150.00824546550.20136647
CTGGAAT150.00824546550.20136643
GAATTCG150.00824546550.20136612
TGGAATG150.00824546550.20136644
TCTTAAA150.00824546550.20136643
Produced by FastQC (version 0.11.5)