##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526066_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 44360 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.38212353471596 39.0 38.0 40.0 36.0 40.0 2 37.72804328223625 39.0 38.0 40.0 35.0 40.0 3 38.060189359783585 39.0 38.0 40.0 35.0 40.0 4 37.49528854824166 39.0 38.0 40.0 33.0 40.0 5 37.47869702434626 39.0 38.0 40.0 34.0 40.0 6 36.919882777276825 39.0 36.0 39.0 33.0 40.0 7 37.25743913435527 39.0 38.0 40.0 33.0 40.0 8 37.15565825067628 39.0 38.0 40.0 33.0 40.0 9 36.36138412984671 39.0 35.0 40.0 31.0 40.0 10 35.63500901713255 39.0 35.0 40.0 29.0 40.0 11 34.395874661857526 38.0 33.0 39.0 24.0 40.0 12 34.05732642019838 38.0 32.0 39.0 22.0 40.0 13 33.75637962128043 38.0 32.0 39.0 21.0 40.0 14 34.33796212804328 38.0 33.0 39.0 23.0 40.0 15 33.18464833183048 38.0 32.0 39.0 18.0 40.0 16 32.84477006311993 37.0 30.0 39.0 19.0 40.0 17 31.98870604147881 36.0 29.0 39.0 16.0 40.0 18 31.353787195671778 36.0 27.0 39.0 15.0 40.0 19 32.45903967538323 36.0 30.0 39.0 18.0 40.0 20 33.012060414788095 37.0 30.0 39.0 21.0 40.0 21 33.27031109107304 38.0 31.0 39.0 20.0 40.0 22 32.54283137962128 37.0 30.0 39.0 17.0 40.0 23 33.270987376014425 38.0 31.0 39.0 19.0 40.0 24 32.595559062218214 37.0 30.0 39.0 17.0 40.0 25 33.04691163210099 38.0 31.0 39.0 19.0 40.0 26 33.406334535617674 38.0 33.0 39.0 19.0 40.0 27 33.66244364292155 38.0 33.0 39.0 21.0 40.0 28 33.457033363390444 38.0 33.0 39.0 20.0 40.0 29 33.08259693417493 38.0 32.0 39.0 18.0 40.0 30 32.85069882777277 37.0 31.0 39.0 18.0 40.0 31 33.53167267808837 38.0 33.0 39.0 19.0 40.0 32 33.45094679891795 38.0 33.0 39.0 19.0 40.0 33 33.16877817853923 38.0 33.0 39.0 19.0 40.0 34 32.98647430117223 38.0 33.0 39.0 18.0 40.0 35 33.02301623083859 38.0 33.0 39.0 18.0 40.0 36 32.30532010820559 36.0 31.0 38.0 12.0 40.0 37 32.0924706943192 36.0 31.0 38.0 11.0 40.0 38 31.39763300270514 36.0 30.0 38.0 4.0 40.0 39 30.253155996393147 35.0 27.0 38.0 4.0 40.0 40 28.239359783588817 34.0 20.0 38.0 4.0 40.0 41 26.141478809738505 31.0 15.0 38.0 4.0 39.0 42 27.25906221821461 32.0 20.0 38.0 4.0 39.0 43 27.282957619477006 33.0 20.0 38.0 4.0 39.0 44 26.25899458972047 31.0 16.0 37.0 4.0 39.0 45 27.14222272317403 33.0 20.0 38.0 4.0 39.0 46 25.18933724075744 30.0 15.0 36.0 4.0 39.0 47 24.257010820559064 29.0 10.0 36.0 4.0 39.0 48 24.967718665464382 30.0 13.0 36.0 4.0 39.0 49 23.932867448151487 29.0 8.0 35.0 4.0 39.0 50 24.52612714156898 29.0 10.0 36.0 4.0 39.0 51 24.700045085662758 30.0 4.0 36.0 4.0 39.0 52 24.52797565374211 30.0 4.0 36.0 4.0 39.0 53 24.276442741208296 30.0 4.0 36.0 4.0 39.0 54 24.003494138863843 30.0 4.0 36.0 4.0 39.0 55 23.696167718665464 30.0 4.0 36.0 4.0 39.0 56 22.88025247971145 29.0 4.0 36.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.5290298015566322 1 2 1.2990053939182644 1 3 1.0427625831747633 1 4 1.5173482850534157 1 5 1.2913474735245174 1 6 2.546001581753494 1 7 2.1105765006076354 1 8 2.265076639262915 1 9 2.853685827736193 1 10 3.6397651714710904 1 11 0.0693655720878752 1 12 -5.854236505169567 1 13 -17.75443654680373 1 14 -4.824835497579397 1 15 1.9865319433646071 1 16 -1.5342111211758365 1 17 -5.553374589020056 1 18 -4.477691125840497 1 19 0.34408085640561836 1 20 -0.5296828168512526 1 21 6.412714307700746 1 22 7.543479563736039 1 23 6.355570159926199 1 24 7.044068175373173 1 25 6.094135682115958 1 26 6.494075573300478 1 27 6.069773345240591 1 28 6.048645599590081 1 29 6.72331341233739 1 30 6.262671562836623 1 31 5.635893958555965 1 32 5.7153746833325485 1 33 5.6268035229191256 1 34 5.606226238880662 1 35 5.31191340386372 1 36 4.759469730650167 1 37 4.61403794502478 1 38 5.888317975724998 1 39 5.17767151953457 1 40 -10.285363376161008 1 41 1.1485884067406111 1 42 -0.4077872169079946 1 43 -8.293854710639497 1 44 -15.634039658128634 1 45 -0.40073625592674844 1 46 -3.9770297374058785 1 47 -8.843137325025204 1 48 -3.93567284757178 1 49 -13.872873564189707 1 50 -1.9961863150232304 1 51 4.674692123709541 1 52 4.7943686395260166 1 53 5.639260017783638 1 54 5.072466036793145 1 55 5.309804525785598 1 56 9.0584395901395 2 1 0.6065116417503305 2 2 1.1881095101410253 2 3 0.3773872805113214 2 4 1.4125056700413055 2 5 0.6453426309095036 2 6 2.442127489743811 2 7 1.8246200841427438 2 8 1.9907424988270748 2 9 2.4771725105449107 2 10 3.03371190270596 2 11 3.2148861532016753 2 12 -1.1568999434262253 2 13 -0.21182153469719367 2 14 3.393082177965397 2 15 4.898638480894871 2 16 3.6653046173229527 2 17 6.286334854079222 2 18 3.78986335357839 2 19 5.3484392099165134 2 20 4.676128321163276 2 21 6.323852322228589 2 22 7.2841575298377315 2 23 6.118524155083588 2 24 6.42372919232233 2 25 5.5977676433750325 2 26 5.855576783954227 2 27 5.895439204804752 2 28 5.391018480946009 2 29 4.471255300957246 2 30 6.162671562836621 2 31 4.676329793907055 2 32 4.781718508998409 2 33 5.270629188778692 2 34 4.103320669873394 2 35 4.720387980134902 2 36 5.389009682224021 2 37 4.995878138729385 2 38 5.331417249332748 2 39 6.014475393626583 2 40 3.9352177376404462 2 41 8.2343026924549 2 42 7.034585664447935 2 43 6.040527855946458 2 44 -4.284402854254541 2 45 7.903137836082941 2 46 6.906021110051746 2 47 3.2009304715849645 2 48 6.562390106423379 2 49 -0.4549558886449141 2 50 7.538922643814544 2 51 9.904232075283392 2 52 9.363133772697928 2 53 10.601487620689209 2 54 9.95624327650259 2 55 8.939102346608841 2 56 10.535921430333204 3 1 0.5636544988931931 3 2 0.97382379585531 3 3 0.7488158519398951 3 4 1.3839342414698805 3 5 1.6024854880523591 3 6 2.299270346886665 3 7 1.9389057984284577 3 8 1.6478853559699331 3 9 2.5628867962591926 3 10 3.3051404741345323 3 11 4.057743296058817 3 12 4.185957199430916 3 13 4.988178465302802 3 14 4.5930821779654 3 15 0.2843527666091603 3 16 5.079590331608664 3 17 6.500620568364937 3 18 7.004149067864105 3 19 5.119867781345086 3 20 5.318985464020415 3 21 6.166709465085731 3 22 7.612728958409161 3 23 6.304238440797871 3 24 7.395157763750898 3 25 5.5977676433750325 3 26 5.627005355382799 3 27 5.2811534905190385 3 28 5.405304195231729 3 29 5.499826729528671 3 30 4.091242991408052 3 31 3.862044079621338 3 32 4.396004223284123 3 33 3.6420577602072655 3 34 3.3747492413019664 3 35 4.020387980134906 3 36 4.674723967938306 3 37 5.45302099587224 3 38 5.959988677904175 3 39 6.171618250769441 3 40 7.378074880497589 3 41 10.920016978169183 3 42 9.406014235876508 3 43 9.4833849988036 3 44 9.844168574316885 3 45 9.30313783608294 3 46 9.334592538623177 3 47 9.458073328727824 3 48 9.962390106423378 3 49 10.516472682783657 3 50 8.524636929528828 3 51 9.304232075283391 3 52 9.1345623441265 3 53 9.387201906403494 3 54 9.470528990788303 3 55 9.110530918037412 3 56 9.264492858904632 4 1 0.25683147117464245 4 2 0.7710519408446501 4 3 0.8601164915987454 4 4 0.8365994866724407 4 5 0.3988607545768801 4 6 2.3028950803621484 4 7 1.5757927920318693 4 8 1.3999109423238778 4 9 2.3396458580928865 4 10 3.046291860062034 4 11 4.25049382910786 4 12 4.546724790049254 4 13 4.591376759545874 4 14 4.390949981803352 4 15 5.367508416928988 4 16 6.112639372120391 4 17 7.569064065166643 4 18 7.771739686200988 4 19 7.1045159689783475 4 20 6.39467842777308 4 21 6.430675349947137 4 22 7.372217231330271 4 23 6.787394091117701 4 24 6.375967998292477 4 25 5.668130116722583 4 26 5.5679435216941044 4 27 5.279234513973201 4 28 5.128331913781835 4 29 6.2224280088463715 4 30 4.501477532985874 4 31 5.198504633992339 4 32 3.947603370405659 4 33 4.751012984087858 4 34 4.576881437464014 4 35 5.238298427896098 4 36 5.003082176893528 4 37 4.818692637663286 4 38 4.751459893255987 4 39 6.763302685737457 4 40 -0.08247949050454295 4 41 10.70082721271076 4 42 8.373391634597187 4 43 8.466327429507224 4 44 9.354829555127122 4 45 8.663265767852657 4 46 11.349304692141303 4 47 11.383020023823772 4 48 10.741281364419116 4 49 10.522016392804979 4 50 11.29372008517915 4 51 8.455618002788722 4 52 9.255031427282152 4 53 9.12344924115829 4 54 9.271808308485532 4 55 9.899230278378564 4 56 10.099674095578408 5 1 0.7058905237379136 5 2 0.5603662389194923 5 3 0.8138262039067641 5 4 1.7236857942649095 5 5 0.5703943907438713 5 6 2.1893324586879075 5 7 2.0774151151986473 5 8 1.7367052317463347 5 9 2.5341083283502925 5 10 3.5933392318985042 5 11 4.202256753615757 5 12 4.9838868060561765 5 13 5.096460038801773 5 14 5.179831660367057 5 15 5.804021503669201 5 16 6.834248716701836 5 17 7.450310009358724 5 18 8.194625258340295 5 19 6.477217677825415 5 20 6.382132461950022 5 21 6.48886267419546 5 22 6.897201008098602 5 23 6.702168047423132 5 24 6.780250931452766 5 25 5.823440521221823 5 26 5.569241380227524 5 27 5.903720778303722 5 28 5.648989495438762 5 29 5.347238737810251 5 30 6.349628084575755 5 31 5.086888800118235 5 32 4.613188488294476 5 33 4.498579499337701 5 34 5.048869324117707 5 35 4.255170588830552 5 36 6.38486889547454 5 37 5.9095427350026775 5 38 7.008435882873119 5 39 8.537042681411265 5 40 9.098571774907523 5 41 11.7862695661609 5 42 11.076614649955186 5 43 10.835351872509605 5 44 10.43940666955498 5 45 10.825498084530146 5 46 12.716166037587982 5 47 13.438611631005255 5 48 11.929470851764997 5 49 13.088315332887177 5 50 12.435402975077483 5 51 11.09719273780927 5 52 10.965825284085096 5 53 11.016808531662296 5 54 11.024566257869047 5 55 10.728129261722714 5 56 11.684575674639621 6 1 -0.2796167226388988 6 2 0.71978652877457 6 3 0.14715953724009978 6 4 0.04252637397505765 6 5 0.7588001878453241 6 6 2.1603469514415323 6 7 1.9904585934595147 6 8 2.0265603042101006 6 9 2.7514996326981205 6 10 2.564353724652129 6 11 4.593561101441843 6 12 4.9838868060561765 6 13 5.458778879381484 6 14 4.455193979207635 6 15 6.0069200543938415 6 16 6.370480600759805 6 17 6.682194067329739 6 18 8.513465838050436 6 19 6.54968144594136 6 20 6.1792339112253885 6 21 6.343935137963577 6 22 6.650824196504399 6 23 6.281878192350664 6 24 6.432424844496243 6 25 6.055324579192838 6 26 5.308371815010133 6 27 5.13560483627473 6 28 4.648989495438762 6 29 4.709557578389955 6 30 5.856874461387349 6 31 3.8405119885240318 6 32 3.613188488294476 6 33 4.03481138339567 6 34 3.932927295132199 6 35 3.8928517482508482 6 36 4.660231214315118 6 37 3.9820065031186154 6 38 5.008435882873119 6 39 6.000810797353289 6 40 0.7797311951973818 6 41 9.699313044421768 6 42 9.00415088183924 6 43 8.60346781453859 6 44 9.12056608984484 6 45 8.883469099022896 6 46 10.78862980570392 6 47 11.56904641361395 6 48 10.465702735822966 6 49 10.17527185462631 6 50 10.246997177976038 6 51 8.271105781287531 6 52 9.34263687828799 6 53 9.103765053401425 6 54 9.502827127434266 6 55 8.423781435635757 6 56 8.554140892030926 7 1 0.3081923140192444 7 2 0.399454047956155 7 3 0.26310156622560754 7 4 0.8087241574362665 7 5 0.6550064964557194 7 6 2.10557286789507 7 7 1.3082335295208978 7 8 1.4899021626926228 7 9 2.372130493738183 7 10 2.419426188420246 7 11 4.306902959924365 7 12 4.1582261069939435 7 13 4.663808717403647 7 14 4.607367892251112 7 15 5.206621674172187 7 16 6.30227940723892 7 17 6.824990316264092 7 18 7.1995272191246045 7 19 6.183313159496343 7 20 6.5794896657010895 7 21 5.217549801220187 7 22 5.895922235720089 7 23 5.111801466007957 7 24 5.985493898204684 7 25 5.085162601358231 7 26 4.406837288155913 7 27 4.515187103964415 7 28 3.796900833887186 7 29 5.175456981629516 7 30 4.203848033424855 7 31 4.3036407182767995 7 32 3.6749958199227777 7 33 3.218528348442561 7 34 3.272228232898609 7 35 4.099799744840787 7 36 3.6747239679383057 7 37 4.085373937048711 7 38 5.014190358576446 7 39 5.677920771777842 7 40 4.193200930917758 7 41 8.839764877328843 7 42 9.296350370330291 7 43 8.256494242501084 7 44 8.272740002888316 7 45 6.95145716381403 7 46 8.27072699240469 7 47 8.433283412761437 7 48 8.572053971969599 7 49 7.324035707993744 7 50 7.622956257259922 7 51 7.457593419821205 7 52 7.218595957571878 7 53 7.547285940016938 7 54 6.624310503393346 7 55 7.525656968457582 7 56 7.499366808484464 8 1 0.7977558813816685 8 2 1.1959436115235178 8 3 0.7792305984836716 8 4 1.570109356677257 8 5 1.0103195894348502 8 6 2.5462749551816017 8 7 1.7688597154791523 8 8 2.2289913467533466 8 9 2.9034397916508965 8 10 3.871039091646054 8 11 5.812121176243153 8 12 5.096556277771931 8 13 5.936104732583914 8 14 5.317045311605952 8 15 6.991726038498559 8 16 6.604935953728479 8 17 7.955920107535441 8 18 8.603227408877927 8 19 7.333692666137708 8 20 6.9457135746194965 8 21 6.438138036514303 8 22 8.435770433063542 8 23 7.105160099784047 8 24 7.130180805225553 8 25 6.445693910656143 8 26 6.580461576580959 8 27 5.694978375311663 8 28 6.20392170675246 8 29 6.092914287132359 8 30 6.730413498320495 8 31 5.138080945980786 8 32 5.684483485956932 8 33 5.187219050529848 8 34 6.166915139919478 8 35 5.965549270457487 8 36 6.48117558084153 8 37 7.227214544259336 8 38 7.342937986659937 8 39 7.376687375193406 8 40 4.212176262986066 8 41 11.27762066480513 8 42 9.150714696706 8 43 9.987993293734476 8 44 10.78886903514638 8 45 10.437700048064507 8 46 11.97376304553562 8 47 12.333188536101094 8 48 12.36285093591647 8 49 11.72062014822145 8 50 12.504360431832975 8 51 11.880268941642836 8 52 11.890322712790091 8 53 11.895957666772158 8 54 12.016151110603971 8 55 11.83495488117105 8 56 10.907810831254864 9 1 0.6868687846074764 9 2 1.080520224426742 9 3 1.1537265662256075 9 4 1.8317020986127375 9 5 1.5355212023380744 9 6 2.634984632600954 9 7 2.1161379412856007 9 8 1.8786889273985068 9 9 3.31422608197348 9 10 3.138176188420246 9 11 5.539439724630249 9 12 4.847564342288059 9 13 6.172999893874234 9 14 5.263617892251112 9 15 6.599084909466303 9 16 7.101911760180094 9 17 8.550174139793505 9 18 8.53316692500696 9 19 7.322099924202227 9 20 6.5712176068775605 9 21 6.188138036514303 9 22 7.499782529837734 9 23 6.518970583655015 9 24 7.354979192322329 9 25 6.416964071946463 9 26 6.378344641097087 9 27 5.433385633376183 9 28 5.825393480946012 9 29 4.903398158100103 9 30 5.887671562836623 9 31 5.8008833653356255 9 32 5.550914937569836 9 33 4.901432760207264 9 34 5.641713527016254 9 35 4.961012980134903 9 36 6.502848967938306 9 37 6.28114599587224 9 38 6.507756535047033 9 39 8.332332536483726 9 40 3.695039166211874 9 41 10.836088406740611 9 42 10.384585664447936 9 43 10.697670713089316 9 44 11.678990002888316 9 45 10.650905693225795 9 46 12.709146110051748 9 47 13.675930471584966 9 48 11.21819367785195 9 49 13.887454825640802 9 50 12.650529786671687 9 51 10.949321360997676 9 52 11.214919486983643 9 53 11.69434476354635 9 54 12.226332562216875 9 55 12.199370203751698 9 56 12.575653573190348 10 1 -0.25063121539252364 10 2 0.9309666529981726 10 3 0.45357775670179734 10 4 0.9886961462317885 10 5 0.46756485313172647 10 6 1.7421274897438082 10 7 1.2706518301744865 10 8 1.5986790067635894 10 9 1.691458224830626 10 10 2.7845055534996135 10 11 4.68948932780485 10 12 4.620877834351553 10 13 5.1357975129218545 10 14 4.186732971616195 10 15 5.79228927454567 10 16 6.389114141132474 10 17 7.241890409634777 10 18 7.432720496435529 10 19 6.510343971821278 10 20 6.247556892591845 10 21 6.176233274609544 10 22 6.476221021901228 10 23 5.815349551908984 10 24 6.585633954227088 10 25 5.296180341787736 10 26 3.8508148791923276 10 27 4.736709046074594 10 28 4.575145465072993 10 29 4.298239427941375 10 30 4.4372747374398 10 31 2.4429964605737204 10 32 3.5055280328079306 10 33 2.2563434744929793 10 34 3.352527019079748 10 35 2.5822927420396624 10 36 4.135041428255768 10 37 4.96889401174526 10 38 4.521893439808938 10 39 5.798602377753568 10 40 7.314582817005526 10 41 7.7358899940422 10 42 6.388553918416189 10 43 7.277035792454399 10 44 7.7568669870153 10 45 6.053931486876589 10 46 8.085386189416827 10 47 8.308866979521476 10 48 6.72588216991544 10 49 9.075202841513818 10 50 7.142097246989145 10 51 7.701057472108786 10 52 8.90440361396777 10 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3.278556087921636 115 28 3.1819275718551054 115 29 3.4374890671910094 115 30 3.8535806537457162 115 31 2.7795765471538054 115 32 3.3875626648425623 115 33 2.723875942025444 115 34 3.610463527016254 115 35 2.820387980134903 115 36 5.561087604301939 115 37 4.793930086781334 115 38 6.34440426231976 115 39 6.070968900120093 115 40 7.981970984393694 115 41 9.716770224922431 115 42 8.930040209902483 115 43 7.174943440362043 115 44 -0.9545327243844106 115 45 7.646644329589428 115 46 4.096930200960838 115 47 -7.744524073869579 115 48 3.6883641323974032 115 49 -0.4406701743591981 115 50 3.9317797866716866 115 51 5.589946360997676 115 52 5.641055850620006 115 53 5.103435672637261 115 54 5.401048471307785 115 55 5.882608840115335 115 56 7.687869482281258 116 1 0.4852178412112522 116 2 0.7854140923512674 116 3 0.9612147737727739 116 4 0.8467374759712314 116 5 1.118657994790908 116 6 2.300078972223595 116 7 1.9478006771346585 116 8 1.443842229285302 116 9 2.0769029687659355 116 10 2.8911243016277908 116 11 3.180654347271755 116 12 2.430701134740893 116 13 3.195995176893099 116 14 3.6168018545152663 116 15 -4.855808958458226 116 16 4.215708929991415 116 17 3.406011403944447 116 18 4.540537208025825 116 19 3.8778192638248683 116 20 4.339201097443599 116 21 5.183421055382226 116 22 5.531327341158491 116 23 4.1867182251644515 116 24 5.993540513077047 116 25 4.686716430437027 116 26 3.952342282606523 116 27 4.795708746583728 116 28 2.930641122455448 116 29 4.632171743005763 116 30 3.432482883591341 116 31 3.1968149691092087 116 32 3.353686163984932 116 33 3.2401709677544304 116 34 2.369897489280408 116 35 2.386425715983961 116 36 4.108686232089248 116 37 3.273775712853375 116 38 3.7303390822168474 116 39 5.353558951578066 116 40 7.210958977532631 116 41 7.110852557684009 116 42 3.1911894380328434 116 43 -1.972140607665402 116 44 -0.774429808432437 116 45 2.4112240894522117 116 46 -2.220393984287874 116 47 -6.991522358603714 116 48 -2.1058039636574826 116 49 -4.67651923096297 116 50 -0.7333145529509544 116 51 2.363531266658054 116 52 2.2868534492477934 116 53 2.4726466503388025 116 54 1.5627122791980064 116 55 2.6849244490347175 116 56 4.033495554322425 117 1 0.27436878460747494 117 2 0.8273952244267448 117 3 0.613101566225609 117 4 1.2660770986127403 117 5 0.9167712023380759 117 6 2.1599846326009526 117 7 1.7067629412856036 117 8 1.8443139273985096 117 9 1.8486010819734773 117 10 2.6944261884202447 117 11 -0.8136852753697497 117 12 -8.62118565771194 117 13 -9.058250106125765 117 14 -0.7926321077488865 117 15 -8.472790090533696 117 16 -5.841838239819907 117 17 0.39704913979350565 117 18 -2.613708074993042 117 19 -0.5622750757977713 117 20 0.2618426068775612 117 21 4.263138036514306 117 22 5.6591575298377315 117 23 4.472095583655015 117 24 5.073729192322329 117 25 4.851339071946466 117 26 5.262719641097085 117 27 4.549010633376184 117 28 5.216018480946012 117 29 5.528398158100103 117 30 5.412671562836621 117 31 5.647758365335623 117 32 4.760289937569837 117 33 4.992057760207267 117 34 5.810463527016257 117 35 5.095387980134902 117 36 7.224723967938303 117 37 7.07802099587224 117 38 7.592131535047034 117 39 8.557332536483727 117 40 11.163789166211874 117 41 9.598588406740614 117 42 8.484585664447938 117 43 -1.6523292869106854 117 44 3.772740002888316 117 45 8.035280693225793 117 46 1.7810211100517463 117 47 0.7009304715849645 117 48 1.9338186778519493 117 49 0.9593298256408005 117 50 3.5567797866716866 117 51 7.639946360997676 117 52 8.452419486983644 117 53 7.51934476354635 117 54 9.591957562216876 117 55 8.9712452037517 117 56 11.153778573190348 118 1 -0.4792026439639514 118 2 0.13096665299816834 118 3 -0.10832700520296612 118 4 0.5696485271841638 118 5 0.3310569166237869 118 6 1.5992703468866694 118 7 0.9246200841427452 118 8 1.0335996416842193 118 9 0.8343153676877648 118 10 1.619426188420249 118 11 -1.3422567039411781 118 12 -7.385471371997657 118 13 -6.911821534697193 118 14 -9.03548925060603 118 15 -11.172790090533699 118 16 -9.820409668391335 118 17 -0.5708080030636395 118 18 -6.938708074993041 118 19 -1.4229893615120588 118 20 -1.6667288216938694 118 21 4.680995179371443 118 22 5.184157529837737 118 23 4.7328098693693015 118 24 5.509443478036612 118 25 4.569196214803608 118 26 4.241291069668513 118 27 3.6382963476618997 118 28 3.9910184809460105 118 29 3.942683872385814 118 30 4.805528705693767 118 31 3.5763297939070533 118 32 3.067432794712694 118 33 3.799200617350124 118 34 4.403320669873398 118 35 3.791816551563471 118 36 5.589009682224017 118 37 5.438735281586528 118 38 6.1599886779041775 118 39 7.100189679340872 118 40 8.80664630906902 118 41 9.691445549597756 118 42 8.591728521590792 118 43 2.0262421416607452 118 44 4.287025717174032 118 45 9.888852121797221 118 46 2.606021110051749 118 47 0.22950190015639294 118 48 2.0338186778519507 118 49 2.059329825640802 118 50 5.767494072385972 118 51 9.647089218140533 118 52 7.20599091555507 118 53 6.972916192117779 118 54 7.6991004193597306 118 55 7.124816632323128 118 56 8.821635716047492 119 1 -0.2776582424195482 119 2 0.17807090010241922 119 3 0.236074539198583 119 4 0.5086446661803024 119 5 0.5735279590948323 119 6 1.4255251731414944 119 7 1.3547359142585762 119 8 1.627422035506612 119 9 1.5486010819734801 119 10 1.7707775397716006 119 11 -0.21098257266704934 119 12 -7.033347819874102 119 13 -3.9427095655852256 119 14 -5.7304699455867265 119 15 -9.464681982425589 119 16 -10.126973374955043 119 17 -0.7036265358821723 119 18 -7.1387080749930405 119 19 -0.6994372379599341 119 20 -0.6597790147440605 119 21 4.586786685162949 119 22 5.686860232540436 119 23 4.766014502573935 119 24 5.121026489619627 119 25 4.5668796124870035 119 26 5.072179100556546 119 27 5.107794417159965 119 28 4.996423886351415 119 29 5.8662359959379415 119 30 5.316725616890679 119 31 3.4828935004707624 119 32 3.6805602078401094 119 33 3.974490192639699 119 34 3.59019325674598 119 35 4.387955547702468 119 36 4.593642886857225 119 37 2.7638318066830543 119 38 3.4009153188308154 119 39 5.524900104051291 119 40 6.961086463509172 119 41 4.06750732565953 119 42 3.594045123907396 119 43 -6.586113070694466 119 44 -2.7678005376522243 119 45 3.5846050175501176 119 46 -3.1831680791374417 119 47 -3.172042501388006 119 48 -6.685100241066969 119 49 -4.45418368787271 119 50 -1.0209229160310151 119 51 4.860216631267946 119 52 4.939581649145804 119 53 5.592993412195 119 54 4.350065670324984 119 55 5.4151641226706175 119 56 6.873373167784941 120 1 -0.08784051771810653 120 2 0.3396045267523249 120 3 0.12356668250468061 120 4 1.010844540473201 120 5 0.6539805046636573 120 6 1.6524264930660735 120 7 1.12594898779723 120 8 0.9379185785612947 120 9 1.4555778261595265 120 10 1.6054727000481535 120 11 -0.4485689962999828 120 12 -5.568860076316593 120 13 -5.948366385195534 120 14 -7.962399549609351 120 15 0.47372153737327594 120 16 -7.875559170052465 120 17 0.6464677444446671 120 18 -7.906149935458156 120 19 0.5790039939696676 120 20 -0.3869946024247639 120 21 2.2869752458166275 120 22 4.495785436814476 120 23 3.2657002348178032 120 24 3.684194308601402 120 25 3.6077344207836752 120 26 5.403417315515689 120 27 4.812382726399441 120 28 4.916599876294846 120 29 4.423746995309408 120 30 5.727787841906391 120 31 4.301246737428652 120 32 5.084708542221001 120 33 5.472290318346801 120 34 5.116277480504625 120 35 4.2855042592046715 120 36 5.558444898170862 120 37 6.046044251686194 120 38 5.57061990714006 120 39 6.864309280669772 120 40 7.826579863886291 120 41 -6.549086011864041 120 42 -3.2840189867148553 120 43 -9.360468821794404 120 44 -15.250515811065172 120 45 -5.158323957936997 120 46 -9.412583541111044 120 47 -8.036278830740615 120 48 -7.021995275636423 120 49 -15.266251569708036 120 50 -5.0391504458864524 120 51 -0.433309452955811 120 52 -0.17606888510938035 120 53 -0.148678492267603 120 54 -0.3161819726668469 120 55 0.35671032003076775 120 56 3.5113367127252317 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 121.0 5 43.0 6 311.0 7 189.0 8 215.0 9 453.0 10 200.0 11 201.0 12 177.0 13 133.0 14 167.0 15 187.0 16 165.0 17 219.0 18 285.0 19 329.0 20 488.0 21 575.0 22 790.0 23 985.0 24 1121.0 25 1284.0 26 1407.0 27 1524.0 28 1629.0 29 1698.0 30 1846.0 31 2050.0 32 2342.0 33 2649.0 34 2884.0 35 3167.0 36 3400.0 37 3597.0 38 3816.0 39 3712.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 99.45897204688909 0.37195671776375117 0.11045987376014427 0.058611361587015326 8 99.2132551848512 0.5275022542831379 0.16456266907123535 0.09467989179440937 9 95.50270513976555 1.6568981064021642 1.8800721370604148 0.9603246167718664 10 48.302524797114515 23.30477908025248 20.063119927862942 8.329576194770064 11 30.493688007213706 30.471145175834085 17.587917042380525 21.447249774571688 12 28.34986474301172 20.069882777276828 19.959422903516682 31.62082957619477 13 20.545536519386832 15.34039675383228 22.560865644724977 41.55320108205591 14 20.640216411181246 24.90306582506763 29.817403065825065 24.63931469792606 15 35.16391399895784 17.832302498923855 22.03266952128503 24.971113980833277 16 32.69946538539622 24.314813561616024 22.15154181047123 20.834179242516523 17 25.190529875986474 18.683201803833146 35.00789177001128 21.11837655016911 18 23.363390441839496 18.34761045987376 28.877366997294864 29.411632100991884 19 30.756393486987506 19.834468449776736 21.32740967931081 28.081728383924947 20 24.903487978327124 26.94660796929676 26.865334687888026 21.28456936448809 21 24.759349850086792 18.079758335399806 29.721138889515093 27.439752924998313 22 22.84039675383228 17.74571686203787 28.38367899008115 31.03020739404869 23 22.98692515779982 19.952660054102793 26.28268710550045 30.777727682596932 24 20.970287208620768 21.67590964425808 28.671265611614587 28.682537535506558 25 31.138818118278355 20.900502784479066 24.674768335851013 23.285910761391563 26 30.82179423571332 20.691443777908077 18.59636462045014 29.890397365928468 27 24.957129963898915 25.169223826714806 18.603339350180505 31.270306859205775 28 26.48616966117361 31.27888365382448 19.416578371920014 22.818368313081898 29 35.78961631399471 23.052776585894627 19.09622637248481 22.061380727625842 30 27.46672682156553 20.897811865553802 30.575231220392514 21.06023009248816 31 27.953279668087216 30.65232586980541 19.745653791517285 21.64874067059009 32 35.931546075624 23.762711100087934 19.674866406619916 20.630876417668148 33 29.01347416886722 22.897059155438193 20.0464937820209 28.042972893673685 34 24.339221976234583 28.385216644828986 26.3701260561153 20.905435322821127 35 23.621051680928407 23.10175882233411 33.91433925628232 19.36285024045517 36 26.771777868001983 28.32814544142193 23.280101051112013 21.619975639464066 37 26.9114392810369 21.081154315133453 31.02334823646299 20.984058167366662 38 34.609214361569926 21.689224303500012 22.389678468942765 21.311882865987304 39 26.338247578624163 20.982999571038313 20.77754949992098 31.901203350416544 40 21.760974833473526 17.71205128788051 28.381112601450432 32.145861277195536 41 19.490521327014218 16.10462996718921 25.316715275246082 39.08813343055049 42 29.260021252063034 23.20996586104768 22.87309805340146 24.656914833487825 43 19.585764030208473 20.33419255641478 31.709853932076154 28.370189481300596 44 21.45914000407581 22.267509000747232 25.113783031044086 31.159567964132872 45 24.970058527105508 21.051680112082796 31.399001197658915 22.57926016315278 46 22.112666500825437 24.419367240326554 23.453718989574618 30.01424726927339 47 19.067585331580617 27.174652100992702 22.16128008703141 31.596482480395267 48 23.596164692611392 28.383530738860692 23.756345177664976 24.263959390862944 49 20.129326912206924 29.00359492640575 22.43550611589681 28.431572045490515 50 28.049606939550014 25.72738772928526 23.122797506099214 23.10020782506551 51 22.05280184602855 30.970748591724544 24.679320408117096 22.29712915412981 52 22.589755755891456 26.44433900449626 29.772475654669105 21.193429584943175 53 22.947187556520166 25.32555615843733 30.00768674262977 21.719569542412735 54 22.469040947301817 28.457470848775195 25.69375395462352 23.379734249299467 55 21.82348551658639 28.00352757614816 26.178685299504785 23.99430160776067 56 28.300266642563383 22.018348623853214 27.04388303882135 22.637501694762054 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 2.5 9 4.0 10 10.5 11 17.0 12 50.0 13 97.5 14 112.0 15 97.0 16 82.0 17 79.0 18 76.0 19 71.5 20 67.0 21 76.5 22 91.0 23 96.0 24 93.5 25 91.0 26 113.0 27 135.0 28 147.5 29 171.0 30 182.0 31 184.0 32 186.0 33 218.0 34 250.0 35 290.5 36 331.0 37 420.0 38 529.0 39 549.0 40 570.0 41 591.0 42 675.5 43 760.0 44 911.5 45 1063.0 46 1385.5 47 2910.0 48 4112.0 49 3855.5 50 3599.0 51 3030.5 52 2462.0 53 2364.0 54 2476.5 55 2687.0 56 2623.5 57 2560.0 58 2626.5 59 2693.0 60 2750.5 61 2808.0 62 2845.0 63 2761.5 64 2641.0 65 2401.5 66 2162.0 67 2025.0 68 1888.0 69 1617.0 70 1346.0 71 1225.0 72 959.5 73 815.0 74 677.5 75 540.0 76 419.0 77 298.0 78 223.5 79 119.5 80 90.0 81 88.0 82 86.0 83 55.0 84 24.0 85 15.0 86 6.0 87 4.0 88 1.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.4981965734896303 16 0.06537421100090171 17 0.022542831379621278 18 0.0 19 0.0405770964833183 20 0.14652840396753833 21 0.002254283137962128 22 0.0 23 0.0 24 0.004508566275924256 25 0.015779981965734897 26 0.0405770964833183 27 0.09017132551848511 28 0.002254283137962128 29 0.17808836789900812 30 0.06762849413886385 31 0.02479711451758341 32 0.02028854824165915 33 0.11947700631199279 34 0.21190261496844004 35 0.15554553651938682 36 0.058611361587015326 37 0.16681695220919748 38 0.2321911632100992 39 0.15103697024346258 40 0.8408476104598738 41 1.0640216411181245 42 0.29080252479711455 43 0.30207394048692515 44 0.44409377817853923 45 0.24120829576194772 46 0.3178539224526601 47 0.5365193868349865 48 0.07889990982867448 49 0.29531109107303877 50 0.2073940486925158 51 0.3539224526600541 52 0.22768259693417495 53 0.2885482416591524 54 0.24346257889990985 55 0.30883678990081154 56 0.23895401262398558 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 44360.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.5653742110009 #Duplication Level Percentage of deduplicated Percentage of total 1 89.15812364088225 64.69792605951308 2 6.561043802423113 9.522091974752028 3 1.7924821373097235 3.9021641118124437 4 0.7611059335197267 2.2091974752028856 5 0.4815159987573781 1.7470694319206492 6 0.28269648959304133 1.2308385933273218 7 0.19571295433364397 0.9941388638412985 8 0.12736874805840323 0.739404869251578 9 0.06834420627524075 0.44634806131650134 >10 0.5187946567256913 6.591523895401262 >50 0.031065548306927617 1.499098286744815 >100 0.018639328984156572 3.2236248872858435 >500 0.0 0.0 >1k 0.003106554830692762 3.196573489630298 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1418 3.196573489630298 No Hit TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 353 0.7957619477006312 No Hit TGTGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 311 0.7010820559062219 No Hit TGTGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 289 0.651487826871055 No Hit TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 198 0.44634806131650134 No Hit TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 162 0.3651938683498647 No Hit TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 117 0.26375112714156895 No Hit TGTGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 95 0.21415689810640215 No Hit TGTGCGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA 79 0.17808836789900812 No Hit TGTGCGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 78 0.17583408476104598 No Hit TGTGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG 65 0.14652840396753833 No Hit TGTGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 64 0.1442741208295762 No Hit TGTGCGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 62 0.13976555455365194 No Hit TGTGCGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 60 0.1352569882777277 No Hit TGTGCGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 56 0.12623985572587917 No Hit TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 55 0.12398557258791704 No Hit TGTGCGGGGGTCCCTAGCCCACTCCCTGTGTTTCCTTTCTCTCTGGAATGGAGGGA 51 0.11496844003606854 No Hit TGTGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 49 0.11045987376014427 No Hit TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 46 0.1036970243462579 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.002254283137962128 0.0 0.0 0.0 0.0 11 0.004508566275924256 0.0 0.0 0.0 0.0 12 0.006762849413886384 0.0 0.0 0.0 0.0 13 0.013525698827772768 0.0 0.0 0.0 0.0 14 0.03606853020739405 0.0 0.0 0.0 0.0 15 0.03606853020739405 0.0 0.0 0.0 0.0 16 0.03606853020739405 0.0 0.0 0.0 0.0 17 0.04283137962128043 0.0 0.0 0.0 0.0 18 0.04508566275924256 0.0 0.0 0.0 0.0 19 0.04959422903516682 0.0 0.0 0.0 0.0 20 0.05410279531109107 0.0 0.0 0.0 0.0 21 0.05410279531109107 0.0 0.0 0.0 0.0 22 0.058611361587015326 0.0 0.0 0.0 0.0 23 0.06086564472497746 0.0 0.0 0.0 0.0 24 0.06086564472497746 0.0 0.0 0.0 0.0 25 0.06311992786293959 0.0 0.0 0.0 0.0 26 0.06311992786293959 0.0 0.0 0.0 0.0 27 0.06311992786293959 0.0 0.0 0.0 0.0 28 0.06988277727682597 0.0 0.0 0.0 0.0 29 0.07664562669071236 0.0 0.0 0.0 0.0 30 0.07889990982867448 0.0 0.0 0.0 0.0 31 0.09242560865644725 0.0 0.0 0.0 0.0 32 0.09467989179440937 0.0 0.0 0.0 0.0 33 0.09918845807033363 0.0 0.0 0.0 0.0 34 0.10144274120829576 0.0 0.0 0.0 0.0 35 0.1036970243462579 0.0 0.0 0.0 0.0 36 0.1036970243462579 0.0 0.0 0.0 0.0 37 0.1036970243462579 0.0 0.0 0.0 0.0 38 0.1036970243462579 0.0 0.0 0.0 0.0 39 0.1036970243462579 0.0 0.0 0.0 0.0 40 0.1036970243462579 0.0 0.0 0.0 0.0 41 0.10820559062218214 0.0 0.0 0.0 0.002254283137962128 42 0.11045987376014428 0.0 0.0 0.0 0.002254283137962128 43 0.11045987376014428 0.0 0.0 0.0 0.002254283137962128 44 0.11947700631199279 0.0 0.0 0.0 0.002254283137962128 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCTGGA 15 0.008024863 50.54639 41 TCAATCT 15 0.008024863 50.54639 41 CGTGCAT 15 0.008024863 50.54639 41 GCGTGCA 20 3.945602E-4 50.488556 40 GATGCGT 20 3.990456E-4 50.373283 37 TAGCCCA 15 0.008208388 50.25854 15 TGGGGAT 15 0.008208388 50.25854 15 GACCGTG 15 0.008208388 50.25854 45 ATTCGTG 15 0.008208388 50.25854 14 GTAAGAA 15 0.008208388 50.25854 46 CTAGCAA 15 0.008208388 50.25854 46 TTAAAGA 15 0.008208388 50.25854 45 GGATGCG 25 2.0062987E-5 50.201366 36 TATGCTC 55 0.0 50.201366 35 TCTGGAA 15 0.008245465 50.201366 42 CACGATT 15 0.008245465 50.201366 47 CTGGAAT 15 0.008245465 50.201366 43 GAATTCG 15 0.008245465 50.201366 12 TGGAATG 15 0.008245465 50.201366 44 TCTTAAA 15 0.008245465 50.201366 43 >>END_MODULE