FastQCFastQC Report
Fri 17 Jun 2016
SRR1526065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6112
Sequences flagged as poor quality0
Sequence length40
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTAC560.9162303664921465No Hit
TGTGCGGGGATGAGATGGACTTAAATGAATTGACGGGTAC550.899869109947644No Hit
TGTGCGGGGATGAGATGGCCTTAAATGAATTGACGGGTAC300.4908376963350785No Hit
TGTGCGGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC230.3763089005235602No Hit
TGTGCGGGGATGAGATGGACTCAAATGAATTGACGGGTAC210.343586387434555No Hit
TGTGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTAC140.22905759162303663No Hit
TGTGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT110.1799738219895288No Hit
TGTGCGGGGATGAGATGGCCTCAAATGAATTGACGGGTAC110.1799738219895288No Hit
TGTGCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAAA100.16361256544502617No Hit
TGTGCGGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC90.14725130890052357No Hit
TGTGCGGGGGAGTGCAGTGCTTGTGCGGGGACACGCCGGG80.13089005235602094No Hit
TGTGCGGGGGTGCAGTGCTTGTGCGGGGACACGCAGGGAT80.13089005235602094No Hit
TGTGCGGGGGTGCAGTGCTTGTGCGGGGACACGCCGGGAT80.13089005235602094No Hit
TGTGCGGGGGAGTGCAGTGCTTGTGCGGGGACACGCAGGG80.13089005235602094No Hit
TGTGCGGGGATGAGCTGGACTTAAATGAATTGACGGGTAC80.13089005235602094No Hit
TGTGCGGGGACACGCAGGGATGAGATGGGGAAAAAAAAAA80.13089005235602094No Hit
TGTGCGGGGATGAGCTGGACTTAAAGGAATTGACGGGTAC70.11452879581151831No Hit
TGTGCGGGGGTGTTGGGTGCGTTGATTTAGATACGTTTAT70.11452879581151831No Hit
TGTGCGGGGATGAGATGGACTTACATGAATTGACGGGTAC70.11452879581151831No Hit
TGTGCGGGGATGAGATGGACTTACAGGAATTGACGGGTAC70.11452879581151831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGGT358.746374E-734.00000432
GGGGGAG358.746374E-734.0000046
TTGACGG358.746374E-734.00000430
ATTGACG358.746374E-734.00000429
TGACGGG358.746374E-734.00000431
CGGGGTT251.8022029E-434.05
GAGTGCA200.002578135834.010
CGGGGTG502.8740033E-1034.05
CGGGGGT406.063783E-834.05
CGGGGGG301.2571785E-534.05
CGGGGCT251.8022029E-434.05
CGGGGCA301.2571785E-534.05
AGGAATT200.002578135834.025
ATGGCCT200.002578135834.015
CGGGTAC251.8022029E-434.034
AGTGCAG200.002578135834.011
ACGGGTA251.8022029E-434.033
GAATTGA406.063783E-834.027
GGGGTGG200.002578135834.06
GGGGCAG200.002578135834.06
Produced by FastQC (version 0.11.5)