##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526065_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6112 Sequences flagged as poor quality 0 Sequence length 40 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.070517015706805 32.0 29.0 33.0 4.0 36.0 2 22.591295811518325 25.0 16.0 31.0 4.0 34.0 3 18.797284031413614 18.0 12.0 27.0 4.0 33.0 4 21.70042539267016 24.0 14.0 30.0 4.0 35.0 5 15.96155104712042 11.0 9.0 24.0 4.0 33.0 6 22.32755235602094 25.0 15.0 31.0 4.0 34.0 7 23.540412303664922 27.0 19.0 31.0 4.0 34.0 8 22.352094240837697 26.0 15.0 31.0 4.0 33.0 9 20.411321989528794 23.0 15.0 27.0 4.0 30.0 10 16.063645287958114 16.0 9.0 23.0 4.0 27.0 11 23.99934554973822 28.0 13.0 34.0 4.0 37.0 12 24.097185863874344 29.0 14.0 34.0 4.0 37.0 13 24.992473821989527 30.0 17.0 35.0 4.0 38.0 14 25.390215968586386 31.0 17.0 35.0 4.0 38.0 15 23.271596858638745 28.0 11.0 34.0 4.0 37.0 16 21.82706151832461 26.0 4.0 33.0 4.0 36.0 17 22.366655759162303 27.0 4.0 33.0 4.0 36.0 18 21.685373036649214 26.0 4.0 33.0 4.0 36.0 19 19.003599476439792 20.0 4.0 30.0 4.0 35.0 20 20.861420157068064 25.0 4.0 33.0 4.0 36.0 21 18.266034031413614 19.0 4.0 30.0 4.0 35.0 22 17.871891361256544 17.0 4.0 31.0 4.0 35.0 23 17.6282722513089 18.0 4.0 30.0 4.0 34.0 24 17.683082460732983 18.0 4.0 30.0 4.0 35.0 25 16.718913612565444 15.0 4.0 30.0 4.0 34.0 26 14.995255235602095 4.0 4.0 28.0 4.0 34.0 27 14.666393979057592 4.0 4.0 28.0 4.0 34.0 28 14.132362565445026 4.0 4.0 27.0 4.0 33.0 29 13.50589005235602 4.0 4.0 26.0 4.0 33.0 30 12.802356020942408 4.0 4.0 24.0 4.0 33.0 31 12.55333769633508 4.0 4.0 25.0 4.0 33.0 32 11.704842931937172 4.0 4.0 21.0 4.0 31.0 33 11.515543193717278 4.0 4.0 21.0 4.0 31.0 34 11.412794502617801 4.0 4.0 21.0 4.0 31.0 35 10.765215968586388 4.0 4.0 18.0 4.0 31.0 36 11.18586387434555 4.0 4.0 17.0 4.0 34.0 37 10.943553664921465 4.0 4.0 17.0 4.0 33.0 38 10.875 4.0 4.0 17.0 4.0 33.0 39 10.342931937172775 4.0 4.0 15.0 4.0 32.0 40 10.49476439790576 4.0 4.0 15.0 4.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -21.543289141414146 1 2 -19.046694624819626 1 3 -15.740310545935543 1 4 -17.908007756132758 1 5 -12.856149290524296 1 6 -18.23811267436267 1 7 -19.408922859547854 1 8 -17.755042087542087 1 9 -16.214005832130827 1 10 -11.985833333333336 1 11 -19.401605339105334 1 12 -19.199653078403077 1 13 -20.24077831890332 1 14 -20.68085467772968 1 15 -18.681299903799893 1 16 -17.061073833573833 1 17 -17.82899831649832 1 18 -16.504972342472353 1 19 -14.463998015873024 1 20 -15.738597883597887 1 21 -13.348296957671945 1 22 -12.570693241943246 1 23 -12.448267797017799 1 24 -12.75394630832131 1 25 -11.983361892736893 1 26 -9.973890091390091 1 27 -9.995703463203464 1 28 -9.501595117845115 1 29 -9.084550264550264 1 30 -8.37118686868687 1 31 -8.136813672438672 1 32 -7.42957341269841 1 33 -7.223417809042806 1 34 -6.962040644540643 1 35 -6.418686568061567 1 36 -6.74721500721501 1 37 -6.697432960557961 1 38 -6.687623256373257 1 39 -6.136491101491101 1 40 -6.39140422077922 2 1 0.9924251443001388 2 2 1.9533053751803742 2 3 -3.740310545935543 2 4 -4.908007756132758 2 5 -6.1061492905242964 2 6 0.2618873256373284 2 7 -3.408922859547854 2 8 7.994957912457913 2 9 5.035994167869173 2 10 -5.235833333333336 2 11 -13.151605339105334 2 12 -14.949653078403077 2 13 -11.24077831890332 2 14 -10.68085467772968 2 15 -7.431299903799893 2 16 -1.3110738335738326 2 17 -2.5789983164983212 2 18 -2.754972342472353 2 19 -6.463998015873024 2 20 -0.2385978835978868 2 21 6.651703042328055 2 22 6.679306758056754 2 23 3.801732202982201 2 24 4.246053691678689 2 25 -2.983361892736893 2 26 -2.223890091390091 2 27 4.754296536796536 2 28 5.998404882154885 2 29 5.4154497354497355 2 30 4.87881313131313 2 31 6.863186327561328 2 32 8.07042658730159 2 33 8.276582190957194 2 34 8.287959355459357 2 35 1.8313134319384332 2 36 7.75278499278499 2 37 1.5525670394420388 2 38 1.0623767436267428 2 39 1.6135088985088988 2 40 -0.3914042207792203 3 1 -0.8825748556998612 3 2 -1.7966946248196258 3 3 -3.865310545935543 3 4 -10.908007756132758 3 5 0.14385070947570355 3 6 -1.9881126743626716 3 7 -0.7839228595478538 3 8 -5.130042087542087 3 9 -1.8390058321308267 3 10 4.889166666666664 3 11 0.598394660894666 3 12 -2.4496530784030774 3 13 -0.24077831890332035 3 14 -7.4308546777296804 3 15 1.5687000962001072 3 16 0.5639261664261674 3 17 3.0460016835016788 3 18 5.120027657527647 3 19 4.286001984126976 3 20 5.511402116402113 3 21 4.776703042328055 3 22 10.054306758056754 3 23 7.051732202982201 3 24 8.24605369167869 3 25 9.641638107263107 3 26 3.776109908609909 3 27 6.004296536796536 3 28 4.998404882154885 3 29 6.6654497354497355 3 30 6.37881313131313 3 31 8.113186327561328 3 32 0.9454265873015899 3 33 3.7765821909571944 3 34 7.162959355459357 3 35 6.831313431938433 3 36 3.8777849927849903 3 37 4.302567039442039 3 38 1.0623767436267428 3 39 2.613508898508899 3 40 1.4835957792207797 4 1 -21.686146284271288 4 2 -16.6181231962482 4 3 -14.311739117364114 4 4 -16.0508648989899 4 5 -11.999006433381439 4 6 -16.809541245791245 4 7 -18.551780002404996 4 8 -16.89789923039923 4 9 -15.356862974987969 4 10 -10.557261904761907 4 11 -17.25874819624819 4 12 -18.199653078403077 4 13 -17.526492604617605 4 14 -19.966568963443965 4 15 -17.967014189514178 4 16 -15.775359547859548 4 17 -15.971855459355464 4 18 -14.219258056758068 4 19 -10.606855158730166 4 20 -13.738597883597887 4 21 -9.776868386243374 4 22 -10.999264670514675 4 23 -8.448267797017799 4 24 -9.611089165464168 4 25 -7.697647607022606 4 26 -7.116747234247234 4 27 -5.8528463203463215 4 28 -5.358737974987973 4 29 -4.941693121693122 4 30 -3.514044011544012 4 31 -4.851099386724386 4 32 -2.5724305555555524 4 33 -2.366274951899948 4 34 -2.8191835016835007 4 35 -3.132972282347281 4 36 -2.318643578643581 4 37 -2.8402901034151045 4 38 -2.2590518278018283 4 39 -1.2793482443482436 4 40 -3.819975649350649 5 1 3.617425144300139 5 2 -4.046694624819626 5 3 -2.865310545935543 5 4 -9.408007756132758 5 5 8.393850709475704 5 6 -2.1131126743626716 5 7 1.7160771404521462 5 8 -4.130042087542087 5 9 -9.464005832130827 5 10 -2.2358333333333356 5 11 0.22339466089466598 5 12 -2.0746530784030774 5 13 0.009221681096679646 5 14 -0.055854677729680446 5 15 1.1937000962001072 5 16 -1.1860738335738326 5 17 2.1710016835016788 5 18 5.370027657527647 5 19 2.786001984126976 5 20 5.136402116402113 5 21 8.526703042328055 5 22 5.054306758056754 5 23 9.176732202982201 5 24 10.37105369167869 5 25 10.766638107263107 5 26 9.526109908609909 5 27 6.879296536796536 5 28 5.248404882154885 5 29 5.9154497354497355 5 30 0.2538131313131302 5 31 3.363186327561328 5 32 3.07042658730159 5 33 4.026582190957194 5 34 2.912959355459357 5 35 0.8313134319384332 5 36 0.6277849927849903 5 37 0.6775670394420388 5 38 0.6873767436267428 5 39 1.2385088985088988 5 40 -0.14140422077922032 6 1 -2.007574855699861 6 2 -4.671694624819626 6 3 0.884689454064457 6 4 -9.408007756132758 6 5 -4.3561492905242964 6 6 0.2618873256373284 6 7 2.966077140452146 6 8 1.869957912457913 6 9 0.9109941678691733 6 10 1.3891666666666644 6 11 2.973394660894666 6 12 -3.0746530784030774 6 13 3.1342216810966796 6 14 2.1941453222703196 6 15 -1.0562999037998928 6 16 -5.311073833573833 6 17 0.04600168350167877 6 18 0.3700276575276469 6 19 5.411001984126976 6 20 -1.6135978835978868 6 21 3.526703042328055 6 22 2.679306758056754 6 23 5.176732202982201 6 24 4.871053691678689 6 25 3.391638107263107 6 26 10.276109908609909 6 27 4.504296536796536 6 28 7.123404882154885 6 29 4.1654497354497355 6 30 2.37881313131313 6 31 7.738186327561328 6 32 8.69542658730159 6 33 6.651582190957194 6 34 4.412959355459357 6 35 4.581313431938433 6 36 6.37778499278499 6 37 7.302567039442039 6 38 4.937376743626743 6 39 5.863508898508899 6 40 5.23359577922078 7 1 2.0424251443001396 7 2 7.453305375180374 7 3 -3.740310545935543 7 4 6.7919922438672415 7 5 -1.8561492905242964 7 6 1.4618873256373277 7 7 0.6910771404521476 7 8 0.9449579124579124 7 9 4.085994167869174 7 10 8.014166666666664 7 11 5.198394660894667 7 12 9.300346921596923 7 13 9.659221681096678 7 14 8.119145322270317 7 15 7.418700096200109 7 16 10.238926166426168 7 17 11.27100168350168 7 18 5.495027657527647 7 19 4.436001984126975 7 20 11.561402116402114 7 21 14.051703042328054 7 22 15.029306758056755 7 23 13.9517322029822 7 24 12.34605369167869 7 25 15.316638107263108 7 26 8.12610990860991 7 27 8.104296536796538 7 28 14.098404882154886 7 29 9.615449735449735 7 30 9.228813131313132 7 31 10.563186327561327 7 32 6.770426587301589 7 33 7.376582190957196 7 34 7.037959355459357 7 35 2.4813134319384336 7 36 4.152784992784991 7 37 4.302567039442039 7 38 0.2123767436267432 7 39 0.7635088985088991 7 40 2.308595779220779 8 1 3.1709965728715694 8 2 6.524733946608947 8 3 -7.311739117364114 8 4 2.5205636724386693 8 5 -4.427577861952868 8 6 5.904744468494471 8 7 5.162505711880719 8 8 4.530672198172198 8 9 5.357422739297746 8 10 1.1570238095238068 8 11 0.7412518037518083 8 12 6.943204064454065 8 13 6.616364538239537 8 14 5.462002465127462 8 15 -0.10987133237131985 8 16 5.938926166426167 8 17 -1.5432840307840365 8 18 4.06645622895622 8 19 4.678859126984118 8 20 7.832830687830686 8 21 5.508845899470913 8 22 8.715021043771038 8 23 10.123160774410774 8 24 12.674625120250116 8 25 2.302352392977392 8 26 8.597538480038482 8 27 0.5757251082251074 8 28 3.7841191678691697 8 29 -1.6559788359788357 8 30 -0.942615440115441 8 31 3.4346148989898992 8 32 3.9989980158730187 8 33 3.9194393338143367 8 34 6.60938792688793 8 35 6.00988486050986 8 36 1.2527849927849903 8 37 5.5882813251563235 8 38 5.74094817219817 8 39 5.43493746993747 8 40 1.4657386363636373 9 1 -2.893938492063498 9 2 0.22603264790764754 9 3 -6.649401455026451 9 4 3.0919922438672423 9 5 -6.3106947450697515 9 6 -1.2381126743626716 9 7 2.318349867724873 9 8 3.6995033670033663 9 9 0.9678123496873567 9 10 0.6505303030303011 9 11 5.052940115440119 9 12 5.436710557960559 9 13 6.395585317460316 9 14 2.046418049543046 9 15 5.591427368927381 9 16 4.484380711880714 9 17 8.898274410774405 9 18 8.67684583934583 9 19 3.9905474386724293 9 20 8.534129389129387 9 21 10.106248496873508 9 22 4.611124939874937 9 23 12.733550384800385 9 24 12.1551446007696 9 25 12.016638107263107 9 26 5.2079280904280925 9 27 6.3679329004328995 9 28 3.407495791245795 9 29 0.00635882635882723 9 30 1.8106313131313119 9 31 3.2268226911976914 9 32 5.3886084054834065 9 33 4.958400372775378 9 34 2.8561411736411753 9 35 5.854040704665707 9 36 12.34369408369408 9 37 12.848021584896586 9 38 8.03964947089947 9 39 8.590781625781625 9 40 8.69950487012987 10 1 2.015152417027412 10 2 1.1351235569985576 10 3 3.0778712722462735 10 4 4.637446789321789 10 5 -5.8561492905242964 10 6 7.034614598364602 10 7 4.136531685906693 10 8 7.5176851851851865 10 9 3.9678123496873567 10 10 2.3778030303030278 10 11 8.598394660894666 10 12 10.254892376142376 10 13 8.486494408369406 10 14 10.046418049543046 10 15 6.136881914381924 10 16 14.120744348244347 10 17 10.534638047138046 10 18 13.9495731120731 10 19 10.081456529581523 10 20 7.98867484367484 10 21 10.197157587782602 10 22 14.883852212602207 10 23 8.551732202982201 10 24 8.700599146224143 10 25 9.653001743626744 10 26 8.207928090428092 10 27 10.3679329004329 10 28 10.952950336700338 10 29 8.824540644540646 10 30 7.356085858585857 10 31 10.954095418470418 10 32 7.752244769119773 10 33 9.140218554593558 10 34 11.31068662818663 10 35 10.12676797739298 10 36 6.980057720057717 10 37 6.484385221260222 10 38 3.857831289081288 10 39 10.045327080327082 10 40 7.154050324675326 11 1 0.1709965728715659 11 2 3.381876803751801 11 3 -7.4545962602212565 11 4 -1.622293470418473 11 5 -3.8561492905242964 11 6 4.476173039923044 11 7 3.876791426166431 11 8 6.67352934102934 11 9 2.7859941678691733 11 10 4.157023809523807 11 11 8.741251803751812 11 12 8.086061207311207 11 13 12.18779310966811 11 14 9.462002465127465 11 15 13.461557239057253 11 16 7.653211880711883 11 17 12.885287397787394 11 18 14.780741943241932 11 19 11.964573412698403 11 20 13.547116402116398 11 21 14.080274470899482 11 22 12.000735329485327 11 23 12.837446488696486 11 24 15.531767977392974 11 25 8.58806667869168 11 26 11.168967051467051 11 27 4.861439393939394 11 28 8.641262025012027 11 29 7.9154497354497355 11 30 3.7716702741702726 11 31 4.434614898989901 11 32 6.141855158730163 11 33 2.3480107623857656 11 34 4.180816498316499 11 35 6.00988486050986 11 36 1.5384992784992768 11 37 3.87399561087061 11 38 3.5980910293410293 11 39 1.1492231842231853 11 40 -0.6771185064935068 12 1 -1.3575748556998626 12 2 0.5533053751803756 12 3 0.8596894540644584 12 4 3.2919922438672415 12 5 -6.556149290524296 12 6 2.9618873256373277 12 7 3.091077140452146 12 8 6.744957912457913 12 9 3.2859941678691733 12 10 3.814166666666665 12 11 11.698394660894667 12 12 10.400346921596924 12 13 9.959221681096682 12 14 9.219145322270318 12 15 10.218700096200106 12 16 11.238926166426165 12 17 14.171001683501679 12 18 15.295027657527644 12 19 12.336001984126977 12 20 14.56140211640211 12 21 17.651703042328055 12 22 11.029306758056755 12 23 15.151732202982203 12 24 14.74605369167869 12 25 18.21663810726311 12 26 1.7261099086099083 12 27 2.1042965367965376 12 28 3.2984048821548857 12 29 2.815449735449736 12 30 5.928813131313131 12 31 0.9631863275613277 12 32 0.6704265873015895 12 33 3.576582190957195 12 34 -0.9620406445406431 12 35 1.381313431938434 12 36 -1.3472150072150093 12 37 -2.3974329605579605 12 38 -3.0876232563732575 12 39 -0.8364911014911005 12 40 -2.191404220779221 13 1 -0.8131304112554183 13 2 -9.602250180375181 13 3 4.148578342953346 13 4 -8.352452200577202 13 5 5.810517376142371 13 6 -6.793668229918227 13 7 -7.408922859547854 13 8 -6.088375420875421 13 9 -5.880672498797493 13 10 -3.43027777777778 13 11 -3.1793831168831126 13 12 -1.1996530784030774 13 13 -2.1296672077922096 13 14 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-8.136813672438672 112 32 -7.42957341269841 112 33 -7.223417809042806 112 34 -6.962040644540643 112 35 -6.418686568061567 112 36 -6.74721500721501 112 37 -6.697432960557961 112 38 -6.687623256373257 112 39 -6.136491101491101 112 40 -6.39140422077922 113 1 3.5757584776334745 113 2 0.619972041847042 113 3 5.093022787397789 113 4 0.9253255772005744 113 5 0.9771840428090357 113 6 2.5952206589706606 113 7 4.591077140452146 113 8 -2.4217087542087548 113 9 3.7859941678691733 113 10 -2.4858333333333356 113 11 2.098394660894666 113 12 6.633680254930255 113 13 5.092555014430012 113 14 5.152478655603652 113 15 4.652033429533439 113 16 2.7722594997594996 113 17 5.504335016835011 113 18 7.328360990860979 113 19 7.536001984126976 113 20 5.428068783068781 113 21 10.485036375661387 113 22 9.595973424723422 113 23 10.718398869648869 113 24 9.412720358345357 113 25 0.5166381072631072 113 26 4.859443241943241 113 27 3.004296536796536 113 28 4.998404882154885 113 29 1.2487830687830694 113 30 6.962146464646462 113 31 2.196519660894662 113 32 2.903759920634924 113 33 3.4432488576238605 113 34 3.871292688792691 113 35 -0.08535323472823286 113 36 0.5861183261183243 113 37 0.6359003727753727 113 38 3.1457100769600768 113 39 1.0301755651755649 113 40 4.275262445887446 114 1 -2.882574855699861 114 2 -0.5466946248196258 114 3 7.759689454064457 114 4 1.4669922438672423 114 5 -6.7311492905242964 114 6 -2.1131126743626716 114 7 -4.283922859547854 114 8 2.994957912457913 114 9 -7.089005832130827 114 10 1.6391666666666644 114 11 0.598394660894666 114 12 -1.5746530784030774 114 13 6.13422168109668 114 14 3.0691453222703196 114 15 3.693700096200107 114 16 5.813926166426167 114 17 6.046001683501679 114 18 5.870027657527647 114 19 10.911001984126976 114 20 -6.238597883597887 114 21 -2.723296957671945 114 22 -0.5706932419432462 114 23 -2.198267797017799 114 24 -5.503946308321311 114 25 0.14163810726310722 114 26 3.276109908609909 114 27 -1.4957034632034638 114 28 0.873404882154885 114 29 -4.4595502645502645 114 30 -1.6211868686868698 114 31 -2.511813672438672 114 32 -0.4295734126984101 114 33 -1.0984178090428056 114 34 -1.712040644540643 114 35 -0.4186865680615668 114 36 -5.24721500721501 114 37 -5.197432960557961 114 38 -5.187623256373257 114 39 -4.636491101491101 114 40 -3.8914042207792203 115 1 1.1709965728715659 115 2 4.524733946608947 115 3 7.688260882635884 115 4 3.377706529581527 115 5 -2.999006433381439 115 6 4.761887325637328 115 7 0.5910771404521462 115 8 4.3878150553150554 115 9 -2.356862974987969 115 10 -0.5572619047619067 115 11 -1.401605339105334 115 12 -2.9139387926887927 115 13 -0.6693497474747474 115 14 -2.3951403920153957 115 15 0.17584295334296485 115 16 3.3674975949975945 115 17 6.885287397787394 115 18 7.637884800384789 115 19 7.250287698412691 115 20 7.9756878306878285 115 21 4.651703042328055 115 22 4.286449615199611 115 23 1.4088750601250588 115 24 1.103196548821547 115 25 6.15949525012025 115 26 -4.973890091390091 115 27 -4.995703463203464 115 28 -4.501595117845115 115 29 -4.0845502645502645 115 30 -3.37118686868687 115 31 -3.136813672438672 115 32 -2.42957341269841 115 33 -2.937703523328519 115 34 -6.962040644540643 115 35 -6.418686568061567 115 36 -6.74721500721501 115 37 -6.697432960557961 115 38 -6.687623256373257 115 39 -6.136491101491101 115 40 -6.39140422077922 116 1 1.7424251443001388 116 2 5.753305375180375 116 3 3.6596894540644556 116 4 -6.308007756132758 116 5 -6.456149290524296 116 6 3.36188732563733 116 7 4.7910771404521455 116 8 3.6449579124579117 116 9 -3.6140058321308253 116 10 3.2141666666666637 116 11 4.598394660894666 116 12 7.600346921596923 116 13 0.7592216810966796 116 14 2.1191453222703203 116 15 1.5187000962001065 116 16 7.538926166426169 116 17 6.9710016835016795 116 18 6.095027657527648 116 19 -0.6639980158730232 116 20 4.661402116402112 116 21 9.451703042328056 116 22 4.829306758056752 116 23 5.151732202982203 116 24 6.246053691678689 116 25 3.016638107263107 116 26 7.82610990860991 116 27 10.404296536796535 116 28 6.098404882154886 116 29 6.715449735449736 116 30 11.228813131313132 116 31 12.263186327561327 116 32 0.5704265873015899 116 33 -4.623417809042806 116 34 -0.16204064454064238 116 35 -0.8186865680615671 116 36 0.05278499278499105 116 37 0.10256703944203949 116 38 -1.0876232563732575 116 39 0.6635088985088995 116 40 -0.19140422077922103 117 1 -7.65757485569986 117 2 -0.24669462481962512 117 3 -5.540310545935544 117 4 -10.908007756132758 117 5 -9.456149290524296 117 6 -5.438112674362671 117 7 -5.608922859547853 117 8 -14.155042087542087 117 9 -5.814005832130826 117 10 -3.585833333333335 117 11 -2.6016053391053333 117 12 -2.999653078403078 117 13 -4.44077831890332 117 14 -4.480854677729681 117 15 -5.681299903799893 117 16 -1.8610738335738333 117 17 -5.428998316498323 117 18 1.8950276575276455 117 19 -1.6639980158730232 117 20 3.061402116402114 117 21 5.051703042328054 117 22 3.8293067580567524 117 23 5.551732202982201 117 24 3.2460536916786893 117 25 4.816638107263108 117 26 2.4261099086099076 117 27 -3.195703463203463 117 28 -3.3015951178451157 117 29 -2.884550264550265 117 30 -1.571186868686869 117 31 -2.5368136724386723 117 32 -2.229573412698411 117 33 -4.423417809042806 117 34 -2.3620406445406434 117 35 -1.4186865680615668 117 36 -5.347215007215009 117 37 -2.8974329605579614 117 38 -2.8876232563732573 117 39 -3.136491101491101 117 40 -0.5914042207792196 118 1 2.4090918109668067 118 2 -3.7133612914862937 118 3 -2.406977212602211 118 4 -1.24134108946609 118 5 -1.8561492905242964 118 6 -4.904779341029339 118 7 -7.742256192881188 118 8 0.2449579124579131 118 9 -11.54733916546416 118 10 -1.9858333333333356 118 11 -6.734938672438666 118 12 -3.5329864117364096 118 13 -11.574111652236654 118 14 -1.3475213443963483 118 15 -18.681299903799893 118 16 -17.061073833573833 118 17 -17.82899831649832 118 18 -16.504972342472353 118 19 -14.463998015873024 118 20 -15.738597883597887 118 21 -13.348296957671945 118 22 -12.570693241943246 118 23 -12.448267797017799 118 24 -12.75394630832131 118 25 -11.983361892736893 118 26 -9.973890091390091 118 27 -9.995703463203464 118 28 -9.501595117845115 118 29 -9.084550264550264 118 30 -8.37118686868687 118 31 -8.136813672438672 118 32 -7.42957341269841 118 33 -7.223417809042806 118 34 -6.962040644540643 118 35 -6.418686568061567 118 36 -6.74721500721501 118 37 -6.697432960557961 118 38 -6.687623256373257 118 39 -6.136491101491101 118 40 -6.39140422077922 119 1 -9.857574855699863 119 2 -6.446694624819624 119 3 -0.1403105459355416 119 4 -8.308007756132758 119 5 -9.056149290524296 119 6 -9.838112674362671 119 7 -12.608922859547853 119 8 -7.155042087542087 119 9 -10.214005832130827 119 10 -3.9858333333333356 119 11 -7.801605339105334 119 12 -11.199653078403077 119 13 -9.44077831890332 119 14 -8.080854677729679 119 15 -9.681299903799893 119 16 -6.661073833573832 119 17 -9.42899831649832 119 18 -5.704972342472352 119 19 -2.8639980158730243 119 20 -10.538597883597888 119 21 -5.1482969576719455 119 22 -3.7706932419432455 119 23 -1.0482677970177985 119 24 -1.3539463083213104 119 25 -0.38336189273689314 119 26 -2.973890091390091 119 27 -2.195703463203463 119 28 -1.501595117845115 119 29 2.115449735449735 119 30 3.22881313131313 119 31 3.4631863275613277 119 32 3.1704265873015895 119 33 3.576582190957195 119 34 -1.962040644540643 119 35 -2.618686568061567 119 36 -1.3472150072150093 119 37 -1.2974329605579609 119 38 -1.2876232563732568 119 39 -0.7364911014911009 119 40 -3.5914042207792205 120 1 5.0757584776334745 120 2 9.28663870851371 120 3 13.926356120731121 120 4 2.4253255772005744 120 5 6.810517376142371 120 6 1.0952206589706606 120 7 -18.07558952621452 120 8 -9.08837542087542 120 9 -7.547339165464161 120 10 -9.652500000000003 120 11 -16.068272005772002 120 12 -11.866319745069744 120 13 -15.24077831890332 120 14 -16.68085467772968 120 15 -16.681299903799893 120 16 -9.727740500240499 120 17 -10.495664983164987 120 18 -8.504972342472353 120 19 -5.13066468253969 120 20 -13.738597883597887 120 21 -7.681630291005279 120 22 -10.570693241943246 120 23 -4.448267797017799 120 24 -7.420612974987977 120 25 -9.983361892736893 120 26 -8.973890091390091 120 27 -2.995703463203464 120 28 -3.501595117845115 120 29 -6.0845502645502645 120 30 -4.704520202020203 120 31 -3.136813672438672 120 32 -6.42957341269841 120 33 -0.8900844757094717 120 34 0.37129268879269084 120 35 0.5813134319384332 120 36 3.9194516594516564 120 37 3.969233706108705 120 38 2.312376743626743 120 39 2.863508898508899 120 40 2.6085957792207797 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 904.0 5 68.0 6 56.0 7 51.0 8 43.0 9 53.0 10 110.0 11 136.0 12 182.0 13 212.0 14 198.0 15 251.0 16 248.0 17 266.0 18 315.0 19 324.0 20 285.0 21 277.0 22 282.0 23 291.0 24 277.0 25 299.0 26 269.0 27 233.0 28 196.0 29 108.0 30 80.0 31 47.0 32 32.0 33 8.0 34 9.0 35 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 99.24701260435424 0.39286298903257494 0.2619086593550499 0.09821574725814373 8 96.69340317564249 1.2604354231461778 0.7693566868554592 1.2768047143558683 9 93.64871501064003 3.4702897364544114 1.5714519561302998 1.3095432967752496 10 30.988219895287962 45.87696335078534 10.029450261780106 13.105366492146597 11 24.18193717277487 24.68913612565445 30.726439790575917 20.402486910994764 12 46.449607329842934 18.75 16.541230366492147 18.259162303664922 13 22.30039267015707 27.65052356020942 22.022251308900522 28.026832460732987 14 35.503926701570684 23.282068062827225 22.414921465968586 18.799083769633505 15 24.721858638743456 29.335732984293195 22.169502617801047 23.772905759162306 16 21.875 22.660340314136125 36.68193717277487 18.782722513089006 17 40.75589005235602 19.846204188481675 18.422774869109947 20.975130890052355 18 39.67604712041885 19.17539267015707 18.717277486910994 22.43128272251309 19 21.58568614576494 20.81418253447144 17.826657912015758 39.773473407747865 20 19.977094240837694 25.11452879581152 22.38219895287958 32.5261780104712 21 22.611256544502616 17.473821989528794 36.82918848167539 23.085732984293195 22 23.1348167539267 15.706806282722512 30.36649214659686 30.791884816753928 23 25.777414075286416 32.12765957446809 22.47135842880524 19.623567921440262 24 24.705497382198953 29.237565445026174 20.81151832460733 25.24541884816754 25 23.25581395348837 35.06387160170325 19.29249918113331 22.387815263675073 26 25.214238628872778 19.462755438365196 33.22346736980884 22.099538562953196 27 36.48715010640039 16.63119986904567 24.095596660664594 22.786053363889344 28 24.14018997707173 27.825090075335734 25.253848673435964 22.780871274156567 29 19.508196721311474 29.16393442622951 27.147540983606554 24.18032786885246 30 16.472709391902967 17.505326995574496 39.518111784953284 26.503851827569253 31 14.573770491803279 22.86885245901639 42.16393442622951 20.39344262295082 32 25.941080196399348 17.168576104746318 34.337152209492636 22.5531914893617 33 16.036655211912944 28.01505481917853 34.47880870561283 21.469481263295695 34 13.530759162303665 19.846204188481675 36.86191099476439 29.76112565445026 35 22.318650728672015 20.02619944326183 37.08858686752907 20.566562960537087 36 23.39937776322253 19.813328966759457 35.991485180939904 20.795808089078108 37 24.34426229508197 18.557377049180328 37.0327868852459 20.065573770491802 38 14.612992963508429 21.10947471772214 45.54082801505482 18.736704303714614 39 13.705583756345177 29.146880628786636 35.51662027181922 21.630915343048958 40 12.352748691099476 21.924083769633505 38.85798429319372 26.8651832460733 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 3.0 15 5.0 16 8.0 17 11.0 18 11.0 19 14.5 20 18.0 21 22.5 22 27.0 23 27.0 24 32.5 25 38.0 26 44.5 27 51.0 28 51.0 29 62.5 30 74.0 31 79.0 32 84.0 33 84.0 34 107.5 35 131.0 36 137.5 37 144.0 38 144.0 39 203.0 40 262.0 41 304.5 42 347.0 43 347.0 44 346.5 45 346.0 46 403.0 47 460.0 48 460.0 49 506.0 50 552.0 51 528.5 52 505.0 53 505.0 54 473.0 55 441.0 56 420.5 57 400.0 58 400.0 59 402.0 60 404.0 61 395.0 62 386.0 63 386.0 64 375.0 65 364.0 66 355.5 67 347.0 68 347.0 69 303.0 70 259.0 71 238.5 72 218.0 73 218.0 74 177.5 75 137.0 76 99.5 77 62.0 78 62.0 79 45.0 80 28.0 81 16.0 82 4.0 83 4.0 84 4.0 85 4.0 86 3.0 87 2.0 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.04908376963350785 8 0.04908376963350785 9 0.04908376963350785 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.32722513089005234 20 0.0 21 0.0 22 0.0 23 0.032722513089005235 24 0.0 25 0.0981675392670157 26 0.7198952879581152 27 0.04908376963350785 28 0.0981675392670157 29 0.1963350785340314 30 0.1799738219895288 31 0.1963350785340314 32 0.032722513089005235 33 0.016361256544502618 34 0.0 35 0.08180628272251309 36 0.08180628272251309 37 0.1963350785340314 38 0.016361256544502618 39 0.08180628272251309 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 6112.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.83965968586386 #Duplication Level Percentage of deduplicated Percentage of total 1 95.57457657985795 85.86387434554975 2 2.93207066108177 5.268324607329842 3 0.6374066654525588 1.7179319371727748 4 0.291385904206884 1.0471204188481675 5 0.16390457111637224 0.7362565445026178 6 0.0364232380258605 0.1963350785340314 7 0.072846476051721 0.45811518324607325 8 0.1092697140775815 0.7853403141361256 9 0.01821161901293025 0.14725130890052357 >10 0.12748133309051174 1.963350785340314 >50 0.0364232380258605 1.8160994764397906 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTAC 56 0.9162303664921465 No Hit TGTGCGGGGATGAGATGGACTTAAATGAATTGACGGGTAC 55 0.899869109947644 No Hit TGTGCGGGGATGAGATGGCCTTAAATGAATTGACGGGTAC 30 0.4908376963350785 No Hit TGTGCGGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC 23 0.3763089005235602 No Hit TGTGCGGGGATGAGATGGACTCAAATGAATTGACGGGTAC 21 0.343586387434555 No Hit TGTGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTAC 14 0.22905759162303663 No Hit TGTGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 11 0.1799738219895288 No Hit TGTGCGGGGATGAGATGGCCTCAAATGAATTGACGGGTAC 11 0.1799738219895288 No Hit TGTGCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAAA 10 0.16361256544502617 No Hit TGTGCGGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC 9 0.14725130890052357 No Hit TGTGCGGGGGAGTGCAGTGCTTGTGCGGGGACACGCCGGG 8 0.13089005235602094 No Hit TGTGCGGGGGTGCAGTGCTTGTGCGGGGACACGCAGGGAT 8 0.13089005235602094 No Hit TGTGCGGGGGTGCAGTGCTTGTGCGGGGACACGCCGGGAT 8 0.13089005235602094 No Hit TGTGCGGGGGAGTGCAGTGCTTGTGCGGGGACACGCAGGG 8 0.13089005235602094 No Hit TGTGCGGGGATGAGCTGGACTTAAATGAATTGACGGGTAC 8 0.13089005235602094 No Hit TGTGCGGGGACACGCAGGGATGAGATGGGGAAAAAAAAAA 8 0.13089005235602094 No Hit TGTGCGGGGATGAGCTGGACTTAAAGGAATTGACGGGTAC 7 0.11452879581151831 No Hit TGTGCGGGGGTGTTGGGTGCGTTGATTTAGATACGTTTAT 7 0.11452879581151831 No Hit TGTGCGGGGATGAGATGGACTTACATGAATTGACGGGTAC 7 0.11452879581151831 No Hit TGTGCGGGGATGAGATGGACTTACAGGAATTGACGGGTAC 7 0.11452879581151831 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGGGT 35 8.746374E-7 34.000004 32 GGGGGAG 35 8.746374E-7 34.000004 6 TTGACGG 35 8.746374E-7 34.000004 30 ATTGACG 35 8.746374E-7 34.000004 29 TGACGGG 35 8.746374E-7 34.000004 31 CGGGGTT 25 1.8022029E-4 34.0 5 GAGTGCA 20 0.0025781358 34.0 10 CGGGGTG 50 2.8740033E-10 34.0 5 CGGGGGT 40 6.063783E-8 34.0 5 CGGGGGG 30 1.2571785E-5 34.0 5 CGGGGCT 25 1.8022029E-4 34.0 5 CGGGGCA 30 1.2571785E-5 34.0 5 AGGAATT 20 0.0025781358 34.0 25 ATGGCCT 20 0.0025781358 34.0 15 CGGGTAC 25 1.8022029E-4 34.0 34 AGTGCAG 20 0.0025781358 34.0 11 ACGGGTA 25 1.8022029E-4 34.0 33 GAATTGA 40 6.063783E-8 34.0 27 GGGGTGG 20 0.0025781358 34.0 6 GGGGCAG 20 0.0025781358 34.0 6 >>END_MODULE