FastQCFastQC Report
Fri 17 Jun 2016
SRR1526063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4083
Sequences flagged as poor quality0
Sequence length40
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1724.212588782757776No Hit
TGTGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT491.2000979671809944No Hit
TGTGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG431.053147195689444No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.612294881214793No Hit
TGTGCGGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT130.31839333823169236No Hit
TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC100.24491795248591722No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC100.24491795248591722No Hit
TGTGCGGGGTACCTGGTTGATACTGCCAGTAGCATATGCT100.24491795248591722No Hit
TGTGCGGGGTACATGGTTGATCCTGCCAGTAGCATATGCT90.2204261572373255No Hit
TGTGCGGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT80.19593436198873376No Hit
TGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.17144256674014205No Hit
TGTGCGGGGGTGCAGTGCTTGTGCGGGGTCACAGATCCTC70.17144256674014205No Hit
TGTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT70.17144256674014205No Hit
TGTGCGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT70.17144256674014205No Hit
TGTGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGG60.1469507714915503No Hit
TGTGCGGGGGAGTGCAGTGCTTGTGCGGGGTCACAGATCC60.1469507714915503No Hit
TGTGCGGGGTCGGCCGCGGCACTGATCCCCACAGGTGATG60.1469507714915503No Hit
TGTGCGGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT50.12245897624295861No Hit
TGTGCGGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG50.12245897624295861No Hit
TGTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG50.12245897624295861No Hit
TGTGCGGGGGGGTAGTATCAAGTGTGGACAGAAAGTATTT50.12245897624295861No Hit
TGTGCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGA50.12245897624295861No Hit
TGTGCGGGGATGCCACAACTAGATACATCAACATGATTTA50.12245897624295861No Hit
TGTGCGGGGGTCCCTAGCCCACTCCCTGTGTTTCCTTTCT50.12245897624295861No Hit
TGTGCGGGGATTGTATGTTTGGCTCAGTGTGCTTGGCTGG50.12245897624295861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGG1800.033.86424
GCGGGGC405.401853E-833.8641974
CGGGGTA301.1847575E-533.8641975
CGGGGGT551.6370905E-1133.8641975
CGGGGGG601.8189894E-1233.8641975
CGGGGGA502.382876E-1033.8641975
CGGGGCG200.002524152433.8641975
CGGGGAT358.032439E-733.8641975
CGGGGAC251.73494E-433.8641975
ATTGGGC200.002524152433.86419733
GATTGGG200.002524152433.86419732
TTGGGCG200.002524152433.86419734
GGGGGTG251.73494E-433.8641976
GGGGGGA200.002524152433.8641976
ATATGCT301.1847575E-533.86419734
GGGGATT200.002524152433.8641976
AGATTGG200.002524152433.86419731
GAGATTG200.002524152433.86419730
TGTGCGG4150.033.048191
TGCGGGG3850.032.9846083
Produced by FastQC (version 0.11.5)