##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526063_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4083 Sequences flagged as poor quality 0 Sequence length 40 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.180749448934606 30.0 4.0 33.0 4.0 35.0 2 14.910849865295127 10.0 4.0 26.0 4.0 33.0 3 15.521430320842518 10.0 4.0 28.0 4.0 34.0 4 13.305167768797453 4.0 4.0 23.0 4.0 33.0 5 13.684300759245653 4.0 4.0 25.0 4.0 33.0 6 11.978202302228754 4.0 4.0 20.0 4.0 31.0 7 13.190301249081557 4.0 4.0 27.0 4.0 33.0 8 12.948322312025471 4.0 4.0 26.0 4.0 33.0 9 10.782757776144992 4.0 4.0 21.0 4.0 28.0 10 9.735978447220182 4.0 4.0 16.0 4.0 27.0 11 9.953710506980162 4.0 4.0 15.0 4.0 28.0 12 9.93877051187852 4.0 4.0 15.0 4.0 29.0 13 9.482733284349743 4.0 4.0 12.0 4.0 28.0 14 9.72103845211854 4.0 4.0 11.0 4.0 30.0 15 9.445505755571883 4.0 4.0 10.0 4.0 29.0 16 8.627969630173892 4.0 4.0 4.0 4.0 26.0 17 8.287533676218466 4.0 4.0 4.0 4.0 25.0 18 8.074455057555719 4.0 4.0 4.0 4.0 24.0 19 7.814107274063189 4.0 4.0 4.0 4.0 23.0 20 8.11511143766838 4.0 4.0 4.0 4.0 26.0 21 7.531716874846926 4.0 4.0 4.0 4.0 22.0 22 7.4281165809453835 4.0 4.0 4.0 4.0 21.0 23 7.398971344599559 4.0 4.0 4.0 4.0 22.0 24 7.190546167034044 4.0 4.0 4.0 4.0 20.0 25 7.450159196669116 4.0 4.0 4.0 4.0 22.0 26 7.557433259857947 4.0 4.0 4.0 4.0 23.0 27 7.615968650502082 4.0 4.0 4.0 4.0 24.0 28 7.75704139113397 4.0 4.0 4.0 4.0 25.0 29 7.212098946852804 4.0 4.0 4.0 4.0 20.0 30 7.488856233161891 4.0 4.0 4.0 4.0 23.0 31 7.9982855743325985 4.0 4.0 4.0 4.0 28.0 32 7.8317413666421745 4.0 4.0 4.0 4.0 26.0 33 7.7462650012245895 4.0 4.0 4.0 4.0 26.0 34 7.8003918687239775 4.0 4.0 4.0 4.0 27.0 35 7.71026206220916 4.0 4.0 4.0 4.0 27.0 36 7.763409257898604 4.0 4.0 4.0 4.0 28.0 37 7.678422728385991 4.0 4.0 4.0 4.0 28.0 38 7.48052902277737 4.0 4.0 4.0 4.0 26.0 39 7.361498897869214 4.0 4.0 4.0 4.0 24.0 40 7.51530737203037 4.0 4.0 4.0 4.0 28.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 8.200808213193536 1 2 15.275557011795545 1 3 -3.861730013106154 1 4 16.868785495849714 1 5 -8.755985146352119 1 6 -7.115967671472257 1 7 -7.364809960681523 1 8 -7.184185233726517 1 9 -5.435954565312363 1 10 -5.069681083442552 1 11 -4.9523809523809526 1 12 -4.85045871559633 1 13 -4.013433813892531 1 14 -4.389252948885977 1 15 -4.368851026649191 1 16 -3.7486238532110088 1 17 -3.72520751419834 1 18 -3.4630406290956763 1 19 -3.2898427260812593 1 20 -3.479685452162518 1 21 -2.9051550895587592 1 22 -2.882830930537353 1 23 -2.8557448667540415 1 24 -2.62649628658803 1 25 -3.0304499781564003 1 26 -2.903363914373087 1 27 -3.068763652249891 1 28 -3.281105286151158 1 29 -2.736041939711664 1 30 -3.05386631716907 1 31 -3.3842070773263426 1 32 -3.312429008300568 1 33 -3.1477719528178243 1 34 -3.5125819134993455 1 35 -3.284600262123197 1 36 -3.1973350808213192 1 37 -3.0783529925731763 1 38 -2.8307776321537803 1 39 -2.8270642201834857 1 40 -2.943752730449977 2 1 5.600808213193535 2 2 -6.724442988204455 2 3 -0.6617300131061548 2 4 -5.531214504150286 2 5 0.6440148536478816 2 6 -0.11596767147225684 2 7 5.2351900393184785 2 8 3.615814766273484 2 9 -3.8359545653123632 2 10 1.9303189165574484 2 11 1.2476190476190467 2 12 9.749541284403671 2 13 7.986566186107469 2 14 5.610747051114023 2 15 9.63114897335081 2 16 0.05137614678899105 2 17 2.6747924858016603 2 18 1.736959370904323 2 19 2.3101572739187404 2 20 5.520314547837482 2 21 2.8948449104412415 2 22 -0.8828309305373532 2 23 4.744255133245958 2 24 2.573503713411969 2 25 4.169550021843599 2 26 4.296636085626912 2 27 5.331236347750109 2 28 3.5188947138488427 2 29 3.8639580602883354 2 30 5.54613368283093 2 31 7.615792922673657 2 32 8.687570991699431 2 33 8.252228047182175 2 34 6.887418086500655 2 35 8.515399737876804 2 36 10.402664919178683 2 37 10.521647007426825 2 38 10.769222367846222 2 39 9.372935779816514 2 40 9.456247269550023 3 1 5.629379641764963 3 2 -5.010157273918741 3 3 -2.433158584534725 3 4 -2.8455002184359994 3 5 2.5297291393621677 3 6 -1.2588248143293992 3 7 -1.6505242463958094 3 8 3.9586719091306257 3 9 4.849759720401924 3 10 -0.6411096548711228 3 11 6.19047619047619 3 12 5.14954128440367 3 13 6.700851900393182 3 14 2.039318479685452 3 15 4.2025775447793805 3 16 8.251376146788992 3 17 7.9890782000873735 3 18 5.108387942332895 3 19 4.853014416775883 3 20 9.806028833551768 3 21 11.523416339012668 3 22 7.545740498034076 3 23 9.287112276103102 3 24 7.944932284840541 3 25 8.255264307557887 3 26 3.096636085626913 3 27 3.3598077763215377 3 28 5.5760375709917 3 29 2.6925294888597646 3 30 4.517562254259501 3 31 5.044364351245086 3 32 5.258999563128003 3 33 6.709370904325033 3 34 8.058846657929227 3 35 4.286828309305374 3 36 6.945522062035823 3 37 4.778789864569681 3 38 6.740650939274791 3 39 6.172935779816514 3 40 8.341961555264309 4 1 2.8008082131935375 4 2 8.275557011795545 4 3 3.5382699868938445 4 4 12.068785495849713 4 5 3.64401485364788 4 6 1.4840323285277428 4 7 1.0351900393184774 4 8 9.615814766273484 4 9 3.7640454346876364 4 10 -1.6696810834425513 4 11 0.8476190476190482 4 12 -0.8504587155963304 4 13 1.7865661861074695 4 14 -3.389252948885977 4 15 1.43114897335081 4 16 -1.7486238532110088 4 17 2.0747924858016606 4 18 2.3369593709043244 4 19 0.5101572739187406 4 20 1.3203145478374827 4 21 2.29484491044124 4 22 -1.682830930537353 4 23 2.944255133245959 4 24 2.573503713411969 4 25 -1.0304499781564003 4 26 1.2966360856269121 4 27 -0.2687636522498913 4 28 1.5188947138488427 4 29 -1.1360419397116646 4 30 1.7461336828309308 4 31 2.0157929226736577 4 32 0.8875709916994312 4 33 2.252228047182176 4 34 -1.3125819134993453 4 35 -0.08460026212319693 4 36 0.6026649191786806 4 37 0.7216470074268235 4 38 0.9692223678462195 4 39 1.3729357798165136 4 40 1.4562472695500235 5 1 3.534141546526868 5 2 7.775557011795545 5 3 3.304936653560514 5 4 5.368785495849714 5 5 -1.0893184796854527 5 6 3.884032328527743 5 7 -2.364809960681523 5 8 -1.3508519003931827 5 9 0.5640454346876371 5 10 3.9303189165574484 5 11 -2.6190476190476195 5 12 -2.0171253822629973 5 13 -2.346767147225864 5 14 -3.889252948885977 5 15 -1.8688510266491907 5 16 -2.9152905198776757 5 17 -2.391874180865007 5 18 -1.1297072957623433 5 19 -0.28984272608125927 5 20 -0.479685452162518 5 21 -2.071821756225426 5 22 -2.382830930537353 5 23 -0.18907820008737453 5 24 -1.793162953254697 5 25 0.8028833551769328 5 26 -0.7366972477064202 5 27 0.5979030144167758 5 28 -1.114438619484491 5 29 -1.2360419397116642 5 30 0.44613368283093013 5 31 1.7824595893403234 5 32 -1.312429008300568 5 33 -0.9811052861511573 5 34 -0.5125819134993455 5 35 -0.11793359545653015 5 36 1.6359982525120147 5 37 1.9216470074268237 5 38 1.1692223678462197 5 39 0.8396024464831813 5 40 1.889580602883357 6 1 1.8008082131935375 6 2 8.675557011795544 6 3 5.138269986893846 6 4 3.668785495849715 6 5 1.2440148536478812 6 6 -2.9159676714722575 6 7 8.635190039318477 6 8 13.815814766273483 6 9 11.164045434687639 6 10 7.730318916557449 6 11 7.647619047619049 6 12 13.14954128440367 6 13 10.58656618610747 6 14 15.210747051114025 6 15 16.43114897335081 6 16 18.051376146788993 6 17 18.07479248580166 6 18 12.936959370904322 6 19 18.31015727391874 6 20 19.520314547837483 6 21 10.49484491044124 6 22 9.317169069462647 6 23 14.94425513324596 6 24 8.37350371341197 6 25 16.9695500218436 6 26 10.896636085626913 6 27 13.331236347750107 6 28 12.918894713848841 6 29 13.263958060288335 6 30 12.146133682830929 6 31 10.415792922673658 6 32 11.88757099169943 6 33 7.052228047182175 6 34 9.687418086500653 6 35 8.715399737876803 6 36 12.002664919178681 6 37 11.721647007426824 6 38 14.16922236784622 6 39 13.172935779816514 6 40 13.656247269550025 7 1 7.200808213193536 7 2 -0.05777632153778889 7 3 6.471603320227178 7 4 -4.797881170816952 7 5 -4.089318479685453 7 6 -6.782634338138924 7 7 -6.364809960681523 7 8 -3.5175185670598497 7 9 0.5640454346876371 7 10 -2.4030144167758847 7 11 0.04761904761904745 7 12 -0.8504587155963304 7 13 -1.0134338138925312 7 14 -3.72258628221931 7 15 0.2978156400174754 7 16 3.584709480122325 7 17 2.941459152468326 7 18 3.2036260375709897 7 19 2.7101572739187407 7 20 5.520314547837482 7 21 1.0948449104412408 7 22 1.1171690694626468 7 23 -0.5224115334207085 7 24 -1.6264962865880301 7 25 1.6362166885102658 7 26 2.763302752293579 7 27 1.9312363477501089 7 28 5.385561380515508 7 29 2.930624726955002 7 30 2.94613368283093 7 31 5.2824595893403234 7 32 1.687570991699432 7 33 1.8522280471821757 7 34 4.4874180865006545 7 35 5.382066404543469 7 36 2.8026649191786808 7 37 5.58831367409349 7 38 -0.8307776321537803 7 39 1.1729357798165143 7 40 6.056247269550023 8 1 -16.465858453473132 8 2 -10.724442988204455 8 3 -10.861730013106154 8 4 -9.131214504150286 8 5 -8.755985146352119 8 6 -7.115967671472257 8 7 -7.364809960681523 8 8 -7.184185233726517 8 9 -5.435954565312363 8 10 -5.069681083442552 8 11 -4.9523809523809526 8 12 -4.85045871559633 8 13 -4.013433813892531 8 14 -4.389252948885977 8 15 -4.368851026649191 8 16 -3.7486238532110088 8 17 -3.72520751419834 8 18 -3.4630406290956763 8 19 -3.2898427260812593 8 20 -3.479685452162518 8 21 -2.9051550895587592 8 22 -2.882830930537353 8 23 -2.8557448667540415 8 24 -2.62649628658803 8 25 -3.0304499781564003 8 26 -2.903363914373087 8 27 -3.068763652249891 8 28 -3.281105286151158 8 29 -2.736041939711664 8 30 -3.05386631716907 8 31 -3.3842070773263426 8 32 -3.312429008300568 8 33 -3.1477719528178243 8 34 -3.5125819134993455 8 35 -3.284600262123197 8 36 -3.1973350808213192 8 37 -3.0783529925731763 8 38 -2.8307776321537803 8 39 -2.8270642201834857 8 40 -2.943752730449977 9 1 3.867474879860204 9 2 4.775557011795545 9 3 3.638269986893846 9 4 9.202118829183046 9 5 3.077348186981215 9 6 7.217365661861075 9 7 8.301856705985145 9 8 0.48248143294014945 9 9 6.730712101354305 9 10 3.7636522498907823 9 11 4.380952380952381 9 12 8.482874617737002 9 13 4.653232852774135 9 14 0.6107470511140232 9 15 0.9644823066841433 9 16 2.4180428134556573 9 17 -0.391874180865007 9 18 4.036959370904324 9 19 -0.45650939274792623 9 20 0.18698121450414895 9 21 1.7615115771079068 9 22 3.117169069462647 9 23 2.6442551332459585 9 24 2.706837046745304 9 25 5.636216688510266 9 26 0.9299694189602459 9 27 -0.7354303189165581 9 28 1.385561380515508 9 29 1.7639580602883358 9 30 4.279467016164264 9 31 0.4491262560069904 9 32 2.354237658366098 9 33 3.018894713848842 9 34 5.3207514198339885 9 35 2.715399737876803 9 36 4.802664919178681 9 37 4.754980340760158 9 38 6.66922236784622 9 39 6.506269113149848 9 40 3.889580602883357 10 1 -7.299191786806464 10 2 -7.224442988204455 10 3 -0.36173001310615405 10 4 -2.631214504150286 10 5 1.7440148536478812 10 6 -5.115967671472257 10 7 3.635190039318477 10 8 3.0658147662734834 10 9 5.564045434687637 10 10 4.430318916557448 10 11 7.0476190476190474 10 12 0.8995412844036696 10 13 4.736566186107469 10 14 5.110747051114023 10 15 3.6311489733508093 10 16 4.251376146788991 10 17 3.77479248580166 10 18 10.036959370904324 10 19 8.46015727391874 10 20 11.770314547837483 10 21 12.34484491044124 10 22 9.117169069462648 10 23 11.14425513324596 10 24 10.87350371341197 10 25 12.469550021843599 10 26 9.846636085626912 10 27 8.431236347750108 10 28 13.218894713848842 10 29 10.763958060288335 10 30 8.19613368283093 10 31 10.615792922673657 10 32 6.687570991699432 10 33 8.102228047182177 10 34 9.487418086500654 10 35 11.215399737876803 10 36 2.5526649191786808 10 37 4.421647007426824 10 38 3.1692223678462197 10 39 2.1729357798165143 10 40 4.556247269550023 11 1 2.200808213193536 11 2 10.475557011795544 11 3 4.938269986893847 11 4 11.068785495849713 11 5 2.4440148536478805 11 6 2.684032328527744 11 7 8.835190039318476 11 8 -6.184185233726517 11 9 -4.435954565312363 11 10 -1.0696810834425516 11 11 -0.7523809523809533 11 12 -1.6504587155963302 11 13 1.5865661861074685 11 14 1.2107470511140228 11 15 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2.2978156400174754 12 16 3.584709480122325 12 17 3.27479248580166 12 18 5.536959370904324 12 19 4.710157273918741 12 20 -1.479685452162518 12 21 4.428178243774575 12 22 3.117169069462647 12 23 4.810921799912625 12 24 5.706837046745304 12 25 5.636216688510266 12 26 6.429969418960247 12 27 7.597903014416775 12 28 4.385561380515508 12 29 6.263958060288336 12 30 7.94613368283093 12 31 4.949126256006991 12 32 4.020904325032766 12 33 3.518894713848842 12 34 5.4874180865006545 12 35 -3.284600262123197 12 36 -3.1973350808213192 12 37 -3.0783529925731763 12 38 -2.8307776321537803 12 39 -2.8270642201834857 12 40 -2.943752730449977 13 1 -20.799191786806464 13 2 -10.724442988204455 13 3 -10.861730013106154 13 4 -9.131214504150286 13 5 -8.755985146352119 13 6 -7.115967671472257 13 7 -7.364809960681523 13 8 -7.184185233726517 13 9 -5.435954565312363 13 10 -5.069681083442552 13 11 -4.9523809523809526 13 12 -4.85045871559633 13 13 -4.013433813892531 13 14 -4.389252948885977 13 15 -4.368851026649191 13 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-2.736041939711664 120 30 -3.05386631716907 120 31 -3.3842070773263426 120 32 -3.312429008300568 120 33 -3.1477719528178243 120 34 -3.5125819134993455 120 35 -3.284600262123197 120 36 -3.1973350808213192 120 37 -3.0783529925731763 120 38 -2.8307776321537803 120 39 -2.8270642201834857 120 40 -2.943752730449977 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 1851.0 5 167.0 6 171.0 7 244.0 8 198.0 9 145.0 10 143.0 11 120.0 12 83.0 13 82.0 14 53.0 15 51.0 16 32.0 17 26.0 18 26.0 19 50.0 20 94.0 21 88.0 22 113.0 23 118.0 24 73.0 25 50.0 26 35.0 27 33.0 28 12.0 29 14.0 30 5.0 31 5.0 32 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 98.97084048027445 0.5390835579514826 0.39206076941926 0.098015192354815 8 97.79411764705883 1.0049019607843137 0.6372549019607843 0.5637254901960784 9 93.84351238655874 2.0848663232769193 2.918812852587687 1.1528084375766494 10 41.82621502209131 24.27589592538046 21.428571428571427 12.4693176239568 11 27.92748652621264 30.156785889269965 21.06810387065164 20.84762371386575 12 29.47806910071061 24.43028669443764 21.24479294290615 24.846851261945602 13 20.455658990690836 21.362077413032825 24.277315041646254 33.90494855463008 14 18.471337579617835 32.288094071533564 31.651151396374328 17.589416952474277 15 37.12956159686505 22.826353171687487 25.055106539309328 14.988978692138133 16 31.912809208915014 29.194219936321332 24.63874602008327 14.254224834680382 17 25.177565515552292 23.63458241489101 35.2192015674749 15.968650502081802 18 22.880940715335623 22.268495835374814 31.504164625183734 23.34639882410583 19 28.2667320421672 25.447413581760237 24.859034076979654 21.426820299092917 20 23.095762919421993 30.982120989468527 29.316678912564292 16.605437178545188 21 20.989468528043105 22.385500857212833 33.87215282880235 22.75287778594171 22 18.148420279206466 20.94048493754592 33.970120009796716 26.940974773450893 23 20.08327210384521 23.75704139113397 30.85966201322557 25.30002449179525 24 18.348848603625672 26.825085742283193 31.773640372366486 23.052425281724645 25 28.757048296151016 26.133856337337587 26.79578328021574 18.31331208629566 26 28.59597447226313 27.491408934707906 19.170348551791854 24.742268041237114 27 19.44922547332186 31.497418244406195 20.998278829604132 28.055077452667813 28 22.35294117647059 38.01470588235294 20.71078431372549 18.92156862745098 29 31.909671084928814 28.694158075601372 21.354933726067745 18.04123711340206 30 24.822347463856897 26.61112472433227 31.68341092869395 16.883116883116884 31 24.93875551200392 36.403723664870164 22.464478196962272 16.193042626163646 32 30.771119842829076 27.431237721021613 23.428290766208253 18.36935166994106 33 24.61425422483468 28.80235121234386 22.703894195444526 23.87950036737693 34 20.818025961302965 31.741366642174874 30.7616948322312 16.67891256429096 35 20.181239284839577 27.822679402400198 37.031594415870686 14.964486896889543 36 21.140609636184855 33.677482792527044 26.474926253687315 18.70698131760079 37 23.78153318638256 26.13274553024737 33.11290717609601 16.97281410727406 38 30.56576047024247 25.104090129806515 26.034778349253003 18.295371050698016 39 22.973303943179037 26.05927014450159 22.532451628704383 28.434974283614988 40 20.64658339456282 24.78569679157482 35.488611315209404 19.079108498652953 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 2.0 15 3.0 16 16.0 17 29.0 18 29.0 19 27.5 20 26.0 21 24.5 22 23.0 23 23.0 24 22.5 25 22.0 26 22.5 27 23.0 28 23.0 29 28.0 30 33.0 31 41.0 32 49.0 33 49.0 34 56.0 35 63.0 36 86.5 37 110.0 38 110.0 39 127.0 40 144.0 41 157.0 42 170.0 43 170.0 44 206.0 45 242.0 46 299.0 47 356.0 48 356.0 49 325.0 50 294.0 51 339.0 52 384.0 53 384.0 54 455.0 55 526.0 56 480.0 57 434.0 58 434.0 59 392.5 60 351.0 61 332.0 62 313.0 63 313.0 64 280.0 65 247.0 66 188.5 67 130.0 68 130.0 69 94.0 70 58.0 71 44.0 72 30.0 73 30.0 74 22.0 75 14.0 76 8.5 77 3.0 78 3.0 79 2.5 80 2.0 81 2.0 82 2.0 83 2.0 84 1.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.04898359049718344 8 0.07347538574577515 9 0.1469507714915503 10 0.2204261572373255 11 0.02449179524859172 12 0.04898359049718344 13 0.02449179524859172 14 0.02449179524859172 15 0.0 16 0.0 17 0.0 18 0.02449179524859172 19 0.09796718099436688 20 0.0 21 0.0 22 0.0 23 0.0 24 0.02449179524859172 25 0.09796718099436688 26 0.2204261572373255 27 0.3918687239774675 28 0.07347538574577515 29 0.2204261572373255 30 0.04898359049718344 31 0.02449179524859172 32 0.26940974773450893 33 0.0 34 0.0 35 0.0 36 0.36737692872887584 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 4083.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.51285819250552 #Duplication Level Percentage of deduplicated Percentage of total 1 92.54971801721578 76.36541758510899 2 4.956960522410211 8.180259613029635 3 1.1872959335114277 2.9390154298310067 4 0.5639655684179281 1.861376438892971 5 0.23745918670228555 0.9796718099436689 6 0.08904719501335707 0.440852314474651 7 0.11872959335114278 0.6857702669605682 8 0.029682398337785694 0.19593436198873376 9 0.029682398337785694 0.2204261572373255 >10 0.20777678836449986 3.9186872397746755 >50 0.0 0.0 >100 0.029682398337785694 4.212588782757776 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 172 4.212588782757776 No Hit TGTGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 49 1.2000979671809944 No Hit TGTGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 43 1.053147195689444 No Hit TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25 0.612294881214793 No Hit TGTGCGGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT 13 0.31839333823169236 No Hit TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC 10 0.24491795248591722 No Hit TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 10 0.24491795248591722 No Hit TGTGCGGGGTACCTGGTTGATACTGCCAGTAGCATATGCT 10 0.24491795248591722 No Hit TGTGCGGGGTACATGGTTGATCCTGCCAGTAGCATATGCT 9 0.2204261572373255 No Hit TGTGCGGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT 8 0.19593436198873376 No Hit TGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.17144256674014205 No Hit TGTGCGGGGGTGCAGTGCTTGTGCGGGGTCACAGATCCTC 7 0.17144256674014205 No Hit TGTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT 7 0.17144256674014205 No Hit TGTGCGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT 7 0.17144256674014205 No Hit TGTGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGG 6 0.1469507714915503 No Hit TGTGCGGGGGAGTGCAGTGCTTGTGCGGGGTCACAGATCC 6 0.1469507714915503 No Hit TGTGCGGGGTCGGCCGCGGCACTGATCCCCACAGGTGATG 6 0.1469507714915503 No Hit TGTGCGGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT 5 0.12245897624295861 No Hit TGTGCGGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG 5 0.12245897624295861 No Hit TGTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 5 0.12245897624295861 No Hit TGTGCGGGGGGGTAGTATCAAGTGTGGACAGAAAGTATTT 5 0.12245897624295861 No Hit TGTGCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGA 5 0.12245897624295861 No Hit TGTGCGGGGATGCCACAACTAGATACATCAACATGATTTA 5 0.12245897624295861 No Hit TGTGCGGGGGTCCCTAGCCCACTCCCTGTGTTTCCTTTCT 5 0.12245897624295861 No Hit TGTGCGGGGATTGTATGTTTGGCTCAGTGTGCTTGGCTGG 5 0.12245897624295861 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.04898359049718344 0.0 0.0 0.0 0.0 15 0.04898359049718344 0.0 0.0 0.0 0.0 16 0.04898359049718344 0.0 0.0 0.0 0.0 17 0.04898359049718344 0.0 0.0 0.0 0.0 18 0.04898359049718344 0.0 0.0 0.0 0.0 19 0.04898359049718344 0.0 0.0 0.0 0.0 20 0.04898359049718344 0.0 0.0 0.0 0.0 21 0.04898359049718344 0.0 0.0 0.0 0.0 22 0.04898359049718344 0.0 0.0 0.0 0.0 23 0.04898359049718344 0.0 0.0 0.0 0.0 24 0.04898359049718344 0.0 0.0 0.0 0.0 25 0.04898359049718344 0.0 0.0 0.0 0.0 26 0.04898359049718344 0.0 0.0 0.0 0.0 27 0.04898359049718344 0.0 0.0 0.0 0.0 28 0.04898359049718344 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGGGGG 180 0.0 33.8642 4 GCGGGGC 40 5.401853E-8 33.864197 4 CGGGGTA 30 1.1847575E-5 33.864197 5 CGGGGGT 55 1.6370905E-11 33.864197 5 CGGGGGG 60 1.8189894E-12 33.864197 5 CGGGGGA 50 2.382876E-10 33.864197 5 CGGGGCG 20 0.0025241524 33.864197 5 CGGGGAT 35 8.032439E-7 33.864197 5 CGGGGAC 25 1.73494E-4 33.864197 5 ATTGGGC 20 0.0025241524 33.864197 33 GATTGGG 20 0.0025241524 33.864197 32 TTGGGCG 20 0.0025241524 33.864197 34 GGGGGTG 25 1.73494E-4 33.864197 6 GGGGGGA 20 0.0025241524 33.864197 6 ATATGCT 30 1.1847575E-5 33.864197 34 GGGGATT 20 0.0025241524 33.864197 6 AGATTGG 20 0.0025241524 33.864197 31 GAGATTG 20 0.0025241524 33.864197 30 TGTGCGG 415 0.0 33.04819 1 TGCGGGG 385 0.0 32.984608 3 >>END_MODULE