SRR1526062_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526062_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18452
Sequences flagged as poor quality	0
Sequence length	56
%GC	40

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	522	2.828961630175591	No Hit
GCTGTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	374	2.026880554953393	No Hit
GCTGTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	328	1.7775850856275743	No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	302	1.636678950791242	No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA	127	0.6882722740082375	No Hit
GCTGTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	94	0.5094298721005853	No Hit
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	90	0.4877520052026881	No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA	83	0.4498157381313679	No Hit
GCTGTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	82	0.4443962714068936	No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	82	0.4443962714068936	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	77	0.417298937784522	No Hit
GCTGTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	77	0.417298937784522	No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	71	0.38478213743767614	No Hit
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	61	0.330587470192933	No Hit
GCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58	0.31432907001951005	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAACAAAAAAAAAAAAAAA	50	0.27097333622371556	No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43	0.2330370691523954	No Hit
GCTGTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	41	0.2221981357034468	No Hit
GCTGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	35	0.18968133535660092	No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACAAAAAAAAAAAAA	34	0.1842618686321266	No Hit
GCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	33	0.1788424019076523	No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	32	0.17342293518317797	No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAA	31	0.16800346845870365	No Hit
GCTGTGGGGAACCCCCACAAAAAAAAAAAAAAAAAAAAAAAACCAACACAAAAAAA	31	0.16800346845870365	No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	30	0.16258400173422935	No Hit
GCTGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	29	0.15716453500975502	No Hit
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA	28	0.15174506828528073	No Hit
GCTGTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	28	0.15174506828528073	No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	27	0.1463256015608064	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAACAAAAAAAAAAAAAAAA	26	0.1409061348363321	No Hit
GCTGTGGGGAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAA	25	0.13548666811185778	No Hit
GCTGTGGGGGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24	0.13006720138738348	No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTCTTTTTTTTTTTTTTT	24	0.13006720138738348	No Hit
GCTGTGGGGGCTGCTGTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAA	23	0.12464773466290917	No Hit
GCTGTGGGGCAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAA	23	0.12464773466290917	No Hit
GCTGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA	22	0.11922826793843486	No Hit
GCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA	20	0.10838933448948623	No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAACAAAAAAAAAAAAAAA	20	0.10838933448948623	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	20	0.10838933448948623	No Hit
GCTGTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	20	0.10838933448948623	No Hit
GCTGTGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC	19	0.10296986776501194	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAATCC	15	0.007890975	50.640884	41
AACCAAC	15	0.007890975	50.640884	41
TTGTTCC	25	1.8916098E-5	50.50138	37
TTGTCCA	15	0.007977854	50.501377	37
TGTCCAA	15	0.007977854	50.501377	38
GGCGCAT	35	4.5418346E-8	50.501377	37
CAACAAA	30	9.2555456E-7	50.501377	40
GTCCAAT	15	0.007977854	50.501377	39
GCGCATT	35	4.5418346E-8	50.501377	38
TCCAATC	15	0.007977854	50.501377	40
ACAACAA	30	9.2555456E-7	50.501377	39
TGGGCGC	35	4.642243E-8	50.36264	35
TTTTGTC	20	3.9326923E-4	50.362637	35
CCAACAC	15	0.0080654435	50.362637	43
CATCCGC	15	0.0080654435	50.362637	43
ACCAACA	15	0.0080654435	50.362637	42
CCGTGCT	15	0.008242765	50.087433	14
CTGGTAT	15	0.008242765	50.087433	15
CCTGGTA	15	0.008242765	50.087433	14
GGATCCT	15	0.008332506	49.950954	9

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content