FastQCFastQC Report
Fri 17 Jun 2016
SRR1526061_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526061_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3491
Sequences flagged as poor quality0
Sequence length40
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAA160.45832139788026355No Hit
GCTGTGGGGTGCTGCTGAGCACCTTTCTGGGATGTTGTCC130.3723861357777141No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAA110.3150959610426812No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.22916069894013177No Hit
GCTGTGGGGGTGCTGCTGCGCACCTTTCTGGGATGTTGTC80.22916069894013177No Hit
GCTGTGGGGGCTGCTGTGGGGCAAAAAAAAAAAAAAAAAA70.20051561157261533No Hit
GCTGTGGGGGTGCTGCTGAGCACCTTTCTGGGATGTTGTC70.20051561157261533No Hit
GCTGTGGGGGTGCTGCTGAGCCCCTTTCTGGGATGTTGTC60.17187052420509882No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAA60.17187052420509882No Hit
GCTGTGGGGGAAAAAAAACAAAAAAAAAAAAAAAAAAAAA60.17187052420509882No Hit
GCTGTGGGGGCTGCTGTGGGGTGCTGCTGAGCACCTTTCT60.17187052420509882No Hit
GCTGTGGGTAACTGAGGTGACATTTAGAGGTCTTTGAGTG50.14322543683758235No Hit
GCTGTGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.14322543683758235No Hit
GCTGTGGGGATCACTCGTTTTTTTTTTTTTTTTTTTTTTT50.14322543683758235No Hit
GCTGTGGGGAAAAAAAAACAAAAAAAAAAAAAAAAAAAAA50.14322543683758235No Hit
GCTGTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC50.14322543683758235No Hit
GCTGTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG50.14322543683758235No Hit
GCTGTGGGGGTGCTGCTGCGCCCCTTTCTGGGATGTTGTC50.14322543683758235No Hit
GCTGTGGGGGCTGCTGAGCACCTTTCTGGGATGTTGTCCT40.11458034947006589No Hit
GCTGTGGGGGGGGTTGGGCATTTAGCTCCGTGGTAGAGCG40.11458034947006589No Hit
GCTGTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11458034947006589No Hit
GCTGTGGGGTCAGTCGACTTTTTTTTTTTTTTTTTTTTTT40.11458034947006589No Hit
GCTGTGGGGGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAA40.11458034947006589No Hit
GCTGTGGGGGTGCTGCTGAGCACCTTTCTGGGCTGTTGTC40.11458034947006589No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11458034947006589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGT404.3122782E-834.397065
TGGGGGG301.0123364E-534.397065
TGGGGGA356.643277E-734.397065
GGGGGTG251.5274389E-434.397066
GTGGGGG1200.034.397064
GTGGGGC251.5274389E-434.397064
GGGGGAG200.002283697734.397066
GGGAAGC356.643277E-734.397067
GGGGAGT200.002283697734.397067
GGGGAAG356.643277E-734.397066
GGAAGCA200.002458611733.898558
TGTGGGG3350.030.8033373
GCTGTGG3750.030.7280391
TGGGGTG451.2167584E-730.5751655
GTGGGGT750.029.8107854
CTGTGGG3750.029.8107852
TGGGGAC304.458511E-428.6642175
TGGGGTA250.006814799327.5176495
GTGGGGA1150.026.9194374
TGGGGAA601.5136575E-622.9313745
Produced by FastQC (version 0.11.5)