FastQCFastQC Report
Fri 17 Jun 2016
SRR1526060_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526060_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1463
Sequences flagged as poor quality0
Sequence length40
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.4101161995898838No Hit
GCTGTGGGGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC60.4101161995898838No Hit
GCTGTGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT50.3417634996582365No Hit
GCTGTGGGGTGCTGCTGAGCCCCTTTCTGGGCTGTTGTCC30.2050580997949419No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAA30.2050580997949419No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2050580997949419No Hit
GCTGTGGGGGTGCTGCTGAGCCCCTTTCTGGGCTGTTGTC30.2050580997949419No Hit
GCTGTGGGGGTGCTGCTGCGCACCTTTCTGGGCTGTTGTC20.1367053998632946No Hit
GCTGTGGGGGTGCTGCTGAGCACCTTTCTGGGCTGTTGTC20.1367053998632946No Hit
GCTGTGGGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTT20.1367053998632946No Hit
GCTGTGGGGTGGACGAGCGTCGTTTTTTTTTTTTTTTTTT20.1367053998632946No Hit
GCTGTGGGGCGTCGCTTTTTTTTTTTTTTTTTTTTTTTTT20.1367053998632946No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAACAAAAA20.1367053998632946No Hit
GCTGTGGGGCGCCTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1367053998632946No Hit
GCTGTGGGGGTGCTGCTGAGCACCTTTCTGGGATGTTGTC20.1367053998632946No Hit
GCTGTGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1367053998632946No Hit
GCTGTGGGGTGCTGCTGACCACCTTTCTGGGATGTTGTCC20.1367053998632946No Hit
GCTGTGGGGGTGCTGCTGCGCACCCTTCTGGGATGTTGTC20.1367053998632946No Hit
GCTGTGGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTT20.1367053998632946No Hit
GCTGTGGGGTGCTGCTGAGCACCTTTCTGGGCTGTTGTCC20.1367053998632946No Hit
GCTGTGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1367053998632946No Hit
GCTGTGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT20.1367053998632946No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAC20.1367053998632946No Hit
GCTGTGGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1367053998632946No Hit
GCTGTGGGGAAAAACAAACAAAAAAAAAAAAAAAAAAAAA20.1367053998632946No Hit
GCTGTGGGGGTGCTGCTGCGCACCTTTCTGGGATGTTGTC20.1367053998632946No Hit
GCTGTGGGGTCGCTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1367053998632946No Hit
GCTGTGGGGAAAAAAAAACAAAAAAAAAAAAAAAAAAAAA20.1367053998632946No Hit
GCTGTGGGGCTCGCTTTTTTTTTTTTTTTTTTTTTTTTTT20.1367053998632946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGGGT305.6281915E-634.9642874
GTGGGGG305.6281915E-634.9642874
TGGGGTG200.001695275734.9642835
TGTGGGG1400.032.466843
GCTGTGG1500.031.4678571
CTGTGGG1500.031.4678572
GTGGGGC351.6248485E-529.9693894
TGGGGAG250.0050731327.971435
GTGGGGA501.14951035E-727.971434
Produced by FastQC (version 0.11.5)