FastQCFastQC Report
Fri 17 Jun 2016
SRR1526059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2701
Sequences flagged as poor quality0
Sequence length40
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2328.589411329137357No Hit
GCTGTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT511.888189559422436No Hit
GCTGTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG431.5920029618659755No Hit
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA391.4439096630877453No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAA270.9996297667530544No Hit
GCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.8885597926693818No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAA240.8885597926693818No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.8885597926693818No Hit
GCTGTGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT220.8145131432802665No Hit
GCTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.7034431691965939No Hit
GCTGTGGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG130.4813032210292484No Hit
GCTGTGGGGAAAAAAAAAAAACAAAAAAAAAAAAAAAAAA130.4813032210292484No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAA120.4442798963346909No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAACAAAA110.40725657164013324No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT110.40725657164013324No Hit
GCTGTGGGGGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAA110.40725657164013324No Hit
GCTGTGGGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.37023324694557574No Hit
GCTGTGGGGAAAAAAAAACAAAAAAAAAAAAAAAAAAAAA80.2961865975564606No Hit
GCTGTGGGGGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAA80.2961865975564606No Hit
GCTGTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.2961865975564606No Hit
GCTGTGGGGGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAA70.259163272861903No Hit
GCTGTGGGGGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAA70.259163272861903No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAA70.259163272861903No Hit
GCTGTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.259163272861903No Hit
GCTGTGGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT60.22213994816734545No Hit
GCTGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT60.22213994816734545No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAA60.22213994816734545No Hit
GCTGTGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.22213994816734545No Hit
GCTGTGGGGAAAACAAAAAAAAAAAAAAAAAAAAAAAAAA60.22213994816734545No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAA60.22213994816734545No Hit
GCTGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG50.18511662347278787No Hit
GCTGTGGGGAAAAAAAACAAAAAAAAAAAAAAAAAAAAAA50.18511662347278787No Hit
GCTGTGGGGAAAAAAAAAAAACACAAAAAAAAAAAAAAAA50.18511662347278787No Hit
GCTGTGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAA50.18511662347278787No Hit
GCTGTGGGGAAAAAACAAAAAAAAAAAAAAAAAAAAAAAA50.18511662347278787No Hit
GCTGTGGGGGCTGCTGTGGGGCAAAAAAAAAAAAAAAAAA50.18511662347278787No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA50.18511662347278787No Hit
GCTGTGGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAA40.1480932987782303No Hit
GCTGTGGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT40.1480932987782303No Hit
GCTGTGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1480932987782303No Hit
GCTGTGGGGAGTGAAGTGCTGCTGTGGGGAAAAAAAAAAA40.1480932987782303No Hit
GCTGTGGGGGCTGCTGTGGGGGAAAAAAAAAAAAAAAAAA40.1480932987782303No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA40.1480932987782303No Hit
GCTGTGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1480932987782303No Hit
GCTGTGGGGGAGTGAAGTGCTGCTGTGGGGAAAAAAAAAA40.1480932987782303No Hit
GCTGTGGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1480932987782303No Hit
GCTGTGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1480932987782303No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAA40.1480932987782303No Hit
GCTGTGGGGCCCAACACCCCCAATAAAAAAACCCAACCCC40.1480932987782303No Hit
GCTGTGGGGTCACAGATCCTCTTATGAGATGAGTTTTTGT40.1480932987782303No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGCAAAAAAAAA40.1480932987782303No Hit
GCTGTGGGGGCTGCTGTGGGGTCACAGATCCTCTTCTGAA40.1480932987782303No Hit
GCTGTGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT40.1480932987782303No Hit
GCTGTGGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG40.1480932987782303No Hit
GCTGTGGGGGCTGGTGTGGGGAAAAAAAAAAAAAAAAAAA40.1480932987782303No Hit
GCTGTGGGGAAAAAAACAAACAAAAAAAAAAAAAAAAAAA40.1480932987782303No Hit
GCTGTGGGGGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGACAAAAAAAAAAAACAAAAAAAAAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGAAAAAAAATAAAAAAAAAAAAAAAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGGCTGCTGTGAGGAAAAAAAAAAAAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGCAGAGTGCAGTGCTGCTGTGGGGAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGGCTGCTGTGCGAAAAAAAAAAAAAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAACAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT30.11106997408367272No Hit
GCTGTGGGGAAAAAAACAAAAAAAAAAAAAAAAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTT30.11106997408367272No Hit
GCTGTGGGGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA30.11106997408367272No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG30.11106997408367272No Hit
GCTGTGGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGTAAAAAAAAAAACAAAAAAAAAAAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGGCGTGCAGTGCTGCTGTGGGGAAAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCT30.11106997408367272No Hit
GCTGTGGGAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGTCACAGATCCTCTTCTGAGATGCGTTTTTGT30.11106997408367272No Hit
GCTGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11106997408367272No Hit
GCTGTGGGGTCACAGATCCCCTTCTGAAGGAGATTGGGCG30.11106997408367272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGTG200.00232683934.05
TGGGGGC200.00232683934.05
GGGGGAG501.5825208E-1034.06
GTGGGGG950.032.2105264
TGGGGGA602.7284841E-1131.1666665
GGGAGTG551.8379069E-827.8181848
GTGGGGT551.8379069E-827.8181844
GGGGAGT551.8379069E-827.8181847
GGAGTGC551.8379069E-827.8181849
TGTGGGG2850.026.2456133
GCTGTGG3600.025.0277771
CTGTGGG3400.025.02
GTGGGGC350.001111024124.2857134
GTGGGGA951.9099389E-1021.4736844
AGTGCAG802.4465407E-821.2511
GAGTGCA854.6644345E-820.010
TGGGGAA854.6644345E-820.05
GTGCAGT806.9835914E-719.12512
TGCTGCT658.059504E-518.30769218
AGTGCTG658.059504E-518.30769216
Produced by FastQC (version 0.11.5)