FastQCFastQC Report
Fri 17 Jun 2016
SRR1526058_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526058_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55488
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4130.7443050749711649No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2600.46856978085351786No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1560.28114186851211076No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1400.25230680507497116No Hit
TCGGGTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT790.1423731257208766No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT770.13876874279123416No Hit
TCGGGTGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCATCCTCTGCTCTTCC660.1189446366782007No Hit
TCGGGTGGGGACGCTACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTC590.10632929642445214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTATA150.00804601350.5297941
TTCGTCA150.00807524350.48351740
ACATGTG150.00807524350.48351740
AACCCTT203.9742474E-450.4373337
ACCCTTG203.9742474E-450.4373338
GGCGCAT150.008104552550.4373337
TATAATT150.008104552550.4373338
AAATATA204.0103245E-450.34520735
AATATAA204.0103245E-450.34520736
TTCGCCC204.0103245E-450.34520735
TCGCCCC150.00816340750.34520336
CCCGTGT204.0284605E-450.2992734
CAAATAT251.9898192E-550.2992734
ACTATTA150.00819295550.29926747
ACCGCTC150.0082225850.25341845
TTATACT204.0832598E-450.16196443
ATTATAC301.0068907E-650.16196442
GTCGGCG150.00828207150.16196448
ATGGGAG150.00840201449.98005330
TTGGCGC150.00840201449.98005329