##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526058_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 55488 Sequences flagged as poor quality 0 Sequence length 56 %GC 57 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.65048298731257 38.0 38.0 39.0 35.0 40.0 2 37.19261822376009 38.0 38.0 39.0 35.0 40.0 3 36.93872549019608 38.0 37.0 38.0 33.0 40.0 4 36.821240628604386 38.0 36.0 38.0 33.0 40.0 5 36.552083333333336 38.0 36.0 38.0 33.0 40.0 6 34.87162990196079 36.0 33.0 39.0 29.0 39.0 7 37.287215253748556 38.0 38.0 39.0 35.0 40.0 8 35.88959775086505 38.0 35.0 39.0 31.0 40.0 9 35.9370134083045 38.0 35.0 39.0 31.0 40.0 10 35.263174019607845 38.0 35.0 39.0 29.0 40.0 11 33.66924380046136 37.0 31.0 39.0 25.0 40.0 12 33.22525591118801 37.0 31.0 39.0 21.0 40.0 13 32.87260308535179 36.0 31.0 38.0 19.0 40.0 14 33.65039287773933 38.0 32.0 38.0 22.0 40.0 15 32.7061526816609 37.0 31.0 38.0 17.0 40.0 16 32.88839028258362 37.0 31.0 39.0 19.0 40.0 17 31.84566032295271 36.0 29.0 38.0 15.0 40.0 18 31.08690167243368 35.0 27.0 38.0 13.0 40.0 19 32.29750576701269 36.0 30.0 38.0 18.0 40.0 20 32.96813725490196 37.0 31.0 39.0 21.0 40.0 21 32.93012903690888 38.0 31.0 39.0 19.0 40.0 22 32.15567329873126 36.0 29.0 39.0 16.0 40.0 23 32.8215830449827 37.0 31.0 39.0 19.0 40.0 24 32.27681660899654 37.0 29.0 39.0 17.0 40.0 25 32.73361807958477 37.0 30.0 39.0 19.0 40.0 26 33.16212514417532 38.0 32.0 39.0 19.0 40.0 27 33.40893526528259 38.0 33.0 39.0 20.0 40.0 28 33.4082684544406 38.0 33.0 39.0 22.0 40.0 29 32.947430074971166 38.0 32.0 39.0 18.0 40.0 30 32.66524293540946 37.0 31.0 39.0 18.0 40.0 31 33.51683246828143 38.0 33.0 39.0 20.0 40.0 32 33.40686274509804 38.0 33.0 39.0 20.0 40.0 33 33.20411620530565 38.0 33.0 39.0 19.0 40.0 34 32.95815311418685 37.0 33.0 39.0 18.0 40.0 35 32.86119521337947 37.0 33.0 39.0 18.0 40.0 36 32.48628532295271 37.0 32.0 38.0 16.0 40.0 37 32.20991926182238 36.0 31.0 38.0 12.0 40.0 38 31.633740628604382 36.0 30.0 38.0 10.0 40.0 39 30.495242214532873 35.0 28.0 38.0 4.0 40.0 40 28.346957900807382 34.0 22.0 38.0 4.0 39.0 41 26.835928489042676 32.0 17.0 38.0 4.0 39.0 42 27.51922938292964 33.0 21.0 38.0 4.0 39.0 43 27.524455738177625 33.0 21.0 38.0 4.0 39.0 44 26.500865051903116 32.0 17.0 38.0 4.0 39.0 45 27.54597390426759 33.0 22.0 38.0 4.0 39.0 46 25.246828143021915 30.0 15.0 36.0 4.0 39.0 47 24.157439446366784 28.0 10.0 36.0 4.0 39.0 48 25.000756920415224 30.0 14.0 36.0 4.0 39.0 49 23.792784025374857 28.0 8.0 36.0 4.0 39.0 50 24.56307670126874 29.0 10.0 36.0 4.0 39.0 51 24.821438869665513 30.0 4.0 36.0 4.0 39.0 52 24.636948529411764 30.0 4.0 36.0 4.0 39.0 53 24.51621972318339 30.0 4.0 36.0 4.0 39.0 54 24.115286188004614 30.0 4.0 36.0 4.0 39.0 55 23.605626441753174 30.0 4.0 36.0 4.0 39.0 56 22.961775519031143 29.0 4.0 36.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.7970228400218957 1 2 1.4458004289915607 1 3 1.7583546113317396 1 4 1.8514577039160613 1 5 2.34668728948634 1 6 4.059598659761349 1 7 2.135734392562341 1 8 3.4988847356442037 1 9 3.209349057713304 1 10 3.9394442255857243 1 11 -2.633695642200415 1 12 -8.861451232061889 1 13 -20.600400870175953 1 14 -8.209039022208643 1 15 -0.3669619542695237 1 16 -0.9282362363283347 1 17 -5.560528781811943 1 18 -3.996058936668554 1 19 -0.010988247209933633 1 20 -0.4769052755198544 1 21 6.248618345929927 1 22 6.9422428041303625 1 23 6.36737897682751 1 24 6.9818800253413045 1 25 6.54771857582007 1 26 6.306102002293375 1 27 6.175291725680413 1 28 6.06811838505406 1 29 6.228490147389085 1 30 6.49523406530253 1 31 4.655519996690256 1 32 5.321544185115265 1 33 4.8672680195927285 1 34 5.250884805585873 1 35 4.890156675191253 1 36 3.8827562172774 1 37 4.370659864839222 1 38 5.16746229751077 1 39 5.044213635697435 1 40 -9.514238672952452 1 41 1.7156489024348076 1 42 1.4240671413969714 1 43 -7.690467864091108 1 44 -14.749503952521833 1 45 -0.4411828295709803 1 46 -3.570194054936138 1 47 -7.673399742158766 1 48 -2.8716475389402873 1 49 -12.688139473691571 1 50 -1.3571154030754684 1 51 5.739156185793465 1 52 5.639599474900503 1 53 6.085950573544036 1 54 6.305106667536339 1 55 6.618907987838206 1 56 8.697739846368606 2 1 1.266123963617403 2 2 1.8296955600777025 2 3 2.2215702346597226 2 4 1.978263482407229 2 5 2.5811709705992385 2 6 4.193226268108063 2 7 2.423455098822373 2 8 4.026172055066354 2 9 3.7718156173708692 2 10 4.388213620984338 2 11 2.845838868232974 2 12 -1.2183265664653113 2 13 1.1544145391338425 2 14 3.929671518187291 2 15 4.696574696345774 2 16 4.99297831690869 2 17 7.270129324127058 2 18 5.860548874995434 2 19 6.279541447814147 2 20 5.5788732156518925 2 21 7.015873562623341 2 22 8.067309684815218 2 23 6.703655060294601 2 24 7.588621598375013 2 25 5.917033717607126 2 26 6.163512382175664 2 27 5.447629874423061 2 28 6.205758834492261 2 29 6.367066926415301 2 30 6.453366756581286 2 31 5.585027754849701 2 32 5.4119668710435676 2 33 5.219194183316645 2 34 5.084485661658643 2 35 4.926406006384408 2 36 5.380080989883066 2 37 5.6022007958183515 2 38 6.2653756201432 2 39 6.718237177698509 2 40 5.0002075549769245 2 41 9.472470732290343 2 42 8.682226584949671 2 43 7.634572264319804 2 44 -2.5349507154966844 2 45 7.861251627357859 2 46 8.119747090061185 2 47 4.387327919692492 2 48 7.396276484601714 2 49 0.789121093456636 2 50 7.196255383441386 2 51 10.205180797885493 2 52 9.876357099298577 2 53 9.730412852837777 2 54 10.173084195626227 2 55 10.295339234494172 2 56 10.258735030959294 3 1 0.8342568825750902 3 2 1.6408358899844728 3 3 1.8057203661442998 3 4 1.655409071697214 3 5 2.099220217551185 3 6 3.142425407481717 3 7 2.0635459427143132 3 8 3.2101309362521064 3 9 3.263807011107424 3 10 2.927539463680965 3 11 3.9339335371308906 3 12 4.3670188793869436 3 13 4.499143202772377 3 14 4.73447668194536 3 15 -0.7679244669341649 3 16 6.305553024056671 3 17 7.1613557166683 3 18 8.062198408822834 3 19 6.666823302942042 3 20 5.852122080103246 3 21 6.765335671157885 3 22 7.987582216491052 3 23 6.833944326371579 3 24 6.492518323213645 3 25 6.2257834187785335 3 26 5.4084322961130304 3 27 5.414400135001589 3 28 5.435139661649803 3 29 5.395156814055749 3 30 5.267017246660178 3 31 4.532672985545371 3 32 4.8225573563412 3 33 4.831553733878444 3 34 3.999618341553454 3 35 4.331139451280411 3 36 5.781945680296648 3 37 5.064428861799708 3 38 5.172021568027493 3 39 6.373747576933507 3 40 8.59822333312658 3 41 10.989205133437846 3 42 10.434198853656344 3 43 9.659076208857218 3 44 9.965288347376848 3 45 9.237895184613414 3 46 11.023524283463047 3 47 10.654361149431914 3 48 11.38037880351159 3 49 11.907098621546524 3 50 11.414161192669212 3 51 10.951415557627303 3 52 10.981544763654302 3 53 11.785038719440692 3 54 11.56574496540868 3 55 12.133852263420778 3 56 10.20685838740204 4 1 0.5020790197971792 4 2 1.560032638729389 4 3 1.7047163020754539 4 4 1.3490499992611618 4 5 2.3676878245318207 4 6 2.968507166984466 4 7 1.9290730763504627 4 8 2.7677450887742197 4 9 3.0190066286068244 4 10 3.6354046322202933 4 11 4.30651302553634 4 12 5.4670666919616515 4 13 5.626324651493395 4 14 4.457761405827739 4 15 5.955001662637915 4 16 6.419944609043519 4 17 8.281365279183238 4 18 8.141447751399934 4 19 6.818867290510774 4 20 6.904715912281109 4 21 7.038345472735699 4 22 6.730231033129822 4 23 7.040733711979996 4 24 6.993115980397484 4 25 6.456359560303753 4 26 5.264635977681284 4 27 5.717292795771375 4 28 5.273174564829343 4 29 6.456954566864734 4 30 5.689321812761062 4 31 3.483904159344082 4 32 4.074888219358179 4 33 4.320317778822265 4 34 3.9496542009844866 4 35 4.690450950204632 4 36 5.155361888759476 4 37 4.725796301436329 4 38 5.714813822390386 4 39 6.471046166462553 4 40 1.3260502516061372 4 41 9.98932466487462 4 42 9.435035573713716 4 43 9.45479698342093 4 44 10.71224029573927 4 45 9.524172975672464 4 46 11.209634730510622 4 47 13.477215560141929 4 48 12.182793338534296 4 49 12.283503115928546 4 50 12.4097385295088 4 51 12.44113585406527 4 52 12.42691889705139 4 53 12.584345437107437 4 54 12.937129139446451 4 55 12.171743728876194 4 56 11.854240648936823 5 1 1.0836082058755565 5 2 1.4620605915931861 5 3 1.7743244138869088 5 4 1.9037225122784278 5 5 2.3887894962226923 5 6 3.5918286249181435 5 7 2.408382476186034 5 8 3.5772819481877534 5 9 3.6846684537644023 5 10 3.903149219778527 5 11 5.0849454727821595 5 12 5.686167815557159 5 13 5.4840939033432115 5 14 5.111228109034101 5 15 6.410331890097492 5 16 6.613285250315101 5 17 7.444697699024292 5 18 8.002779623145617 5 19 7.2921476412918125 5 20 6.598878696605578 5 21 6.5325904713654666 5 22 6.8025796217842505 5 23 5.872605457663745 5 24 6.81114831802423 5 25 6.162991514263744 5 26 5.49483603249082 5 27 4.759496139153125 5 28 5.1839720435906465 5 29 5.200034862836237 5 30 5.674386213966873 5 31 4.842221506562499 5 32 5.216433848297612 5 33 3.9900903192442954 5 34 4.8426385802664775 5 35 5.442711843600083 5 36 5.603429852585176 5 37 6.240868924072668 5 38 6.529831635489867 5 39 7.4759790247799 5 40 8.879489550044067 5 41 10.957809181180455 5 42 11.09247596834238 5 43 11.119636665525615 5 44 11.345153422623348 5 45 10.88605294278674 5 46 12.408319301625994 5 47 12.939259955866788 5 48 13.107156177668308 5 49 13.199781548375789 5 50 13.319713865217217 5 51 12.273418671275461 5 52 12.146568116015484 5 53 11.706159632777485 5 54 11.396570082170488 5 55 10.803286617338784 5 56 11.117600473546304 6 1 0.6916011532749096 6 2 1.3634711117224896 6 3 1.7843155981360965 6 4 1.7808897176854188 6 5 2.2650751265488793 6 6 3.081543582308683 6 7 2.2764400724246485 6 8 2.802671310515116 6 9 3.281494783473484 6 10 3.517900909464096 6 11 5.070693342309049 6 12 5.258456972183662 6 13 5.724470042044356 6 14 4.997357994429336 6 15 6.7398940411406905 6 16 5.929623775146133 6 17 6.6468722508902935 6 18 7.882885412155314 6 19 6.6447776736162325 6 20 6.289445843240902 6 21 6.370821444036636 6 22 7.216626051405175 6 23 6.056707720373119 6 24 7.5240487000400975 6 25 5.46610641288261 6 26 5.543910378655969 6 27 5.451826435950064 6 28 5.4265521199938185 6 29 5.895156814055749 6 30 5.896983921871662 6 31 4.663408694337992 6 32 4.588899319940282 6 33 3.9821561435169954 6 34 4.151630646090755 6 35 4.892021276453445 6 36 4.949595000983653 6 37 5.559558315785861 6 38 6.310319440367916 6 39 7.3719531652441965 6 40 4.722422256479561 6 41 11.244908799164588 6 42 10.175931742659163 6 43 10.69765606017739 6 44 11.315326799055903 6 45 10.202391467617772 6 46 12.467384832040338 6 47 12.195504446022532 6 48 12.810653092155526 6 49 13.32276127214893 6 50 12.338667729454649 6 51 12.051261750911078 6 52 11.841836996415136 6 53 13.374246614852126 6 54 12.470769318138746 6 55 12.987095021687892 6 56 12.302731240516628 7 1 0.8089276019266549 7 2 1.314848048039181 7 3 1.8893069922841192 7 4 1.6728862753446307 7 5 2.1324015752006247 7 6 3.3572177073804 7 7 2.3500201068480564 7 8 3.6060275927870578 7 9 3.864110962475209 7 10 3.975158511300016 7 11 5.794875786371016 7 12 5.995691625080969 7 13 6.64959912982405 7 14 5.529056215886598 7 15 6.859228521920951 7 16 6.488430430338333 7 17 7.534709313426152 7 18 8.622988682379063 7 19 6.48901175279007 7 20 6.534999486384908 7 21 6.962904060215635 7 22 7.275576137463691 7 23 6.284045643494174 7 24 7.612832406293684 7 25 6.892956671058165 7 26 5.615625811817186 7 27 6.008625059013749 7 28 5.306213623149301 7 29 4.88325205215099 7 30 6.530948351016814 7 31 4.143615234785496 7 32 4.84535370892479 7 33 5.033934686259393 7 34 4.488980043681114 7 35 4.354442389476969 7 36 5.07323240775359 7 37 5.442088436267792 7 38 5.369843249891726 7 39 7.568023159506961 7 40 8.402427993714216 7 41 10.620410807196716 7 42 10.781209998539826 7 43 10.154770231146987 7 44 11.440972237954355 7 45 9.820721932333782 7 46 12.084567849825767 7 47 12.969457400698374 7 48 13.306923889631143 7 49 11.704717669165575 7 50 12.880979835019772 7 51 12.822489519126801 7 52 11.044361379662408 7 53 11.669283906877368 7 54 11.74558285801253 7 55 12.428431797362016 7 56 9.650120798749558 8 1 1.0946418876409467 8 2 1.8386575718487066 8 3 1.9488308018079294 8 4 1.9466957991541562 8 5 2.299068241867296 8 6 3.869122469285159 8 7 2.4095439163718595 8 8 3.7845990213584884 8 9 3.6379204862847274 8 10 4.14182517796668 8 11 6.282971024466249 8 12 6.400453529842874 8 13 6.959122939347861 8 14 5.8504847873151675 8 15 6.978276140968571 8 16 7.131287573195472 8 17 8.248995027711867 8 18 8.432512491902873 8 19 6.810440324218639 8 20 6.975475676861098 8 21 7.260523107834686 8 22 8.168433280320837 8 23 7.0935694530179845 8 24 7.5294990729603555 8 25 7.381051909153406 8 26 5.937054383245755 8 27 4.984815535204223 8 28 5.889546956482629 8 29 6.1927758616748 8 30 6.542853112921581 8 31 5.274567615737876 8 32 5.59535370892479 8 33 5.343458495783203 8 34 5.358027662728734 8 35 5.6520614370960125 8 36 6.168470502991685 8 37 6.001612245791602 8 38 6.441271821320296 8 39 7.127546969030771 8 40 2.592904184190406 8 41 11.41802985481576 8 42 10.650257617587446 8 43 10.571436897813651 8 44 11.22668652366864 8 45 10.261198122809972 8 46 12.417901183159096 8 47 12.98136216260314 8 48 12.497400080107333 8 49 13.061860526308429 8 50 11.761932215972152 8 51 10.78677523341251 8 52 11.615789951090978 8 53 11.097855335448799 8 54 11.79320190563158 8 55 11.868907987838206 8 56 10.61440651303527 9 1 0.4041656971647498 9 2 1.1838956670868015 9 3 1.5321641351412651 9 4 1.232410084868441 9 5 1.7752587180577706 9 6 2.5476938978565897 9 7 2.0166867735147207 9 8 3.0345990213584884 9 9 3.1498252481894937 9 10 3.177539463680965 9 11 4.866304357799585 9 12 4.686167815557159 9 13 4.89959912982405 9 14 4.779056215886598 9 15 5.525895188587615 9 16 5.797954239862143 9 17 7.165661694378532 9 18 7.408702968093348 9 19 5.98901175279007 9 20 6.118332819718237 9 21 6.522427869739445 9 22 7.263671375558932 9 23 5.795950405398941 9 24 6.743784787246064 9 25 6.035813813915311 9 26 4.889435335626704 9 27 4.99672029710899 9 28 4.93716600410168 9 29 5.1927758616748 9 30 5.971424541493004 9 31 4.01266285383311 9 32 3.988210851781929 9 33 4.605363257687969 9 34 4.227075281776354 9 35 3.5925376275722023 9 36 5.227994312515495 9 37 5.370659864839222 9 38 5.47698610703458 9 39 6.615642207126012 9 40 1.997666088952311 9 41 8.953744140530045 9 42 9.281209998539826 9 43 9.309532135908892 9 44 9.917162714144837 9 45 8.761198122809972 9 46 10.596472611730526 9 47 11.62421930546028 9 48 10.402161984869238 9 49 10.99043195487986 9 50 11.119075073115006 9 51 10.15582285246013 9 52 10.353885189186219 9 53 10.859760097353558 9 54 11.067011429441099 9 55 10.761765130695348 9 56 10.078692227320985 10 1 0.2906125836116402 10 2 0.8175953007864365 10 3 1.0907721937493235 10 4 1.4906518431102 10 5 1.7999839927830479 10 6 2.2253495755122685 10 7 1.662291169119115 10 8 2.8102400469995175 10 9 2.8750999734642164 10 10 3.0813856175271184 10 11 4.966121207616432 10 12 5.69898832837767 10 13 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2.5222967875547013 114 47 -1.9462935150525382 114 48 1.228169310876563 114 49 -5.631362916915014 114 50 2.546730750770685 114 51 3.3343942810315603 114 52 3.6231159584169887 114 53 3.6381483757418387 114 54 4.112798975228646 114 55 4.578614947545166 114 56 5.579607978236737 115 1 0.7884021503667213 115 2 1.417064796807658 115 3 1.7760064996732794 115 4 1.7430833196796058 115 5 1.982565778813104 115 6 3.1174804331603667 115 7 1.5778526191633233 115 8 2.42253005584125 115 9 2.514357267893928 115 10 2.9102980843706234 115 11 -0.804877908210269 115 12 -3.2448666672014603 115 13 -3.202617618944423 115 14 -4.822750023850677 115 15 -6.477388883661973 115 16 2.472831087152784 115 17 2.759668262523032 115 18 3.810180800605668 115 19 3.5431989449082977 115 20 2.2607794535441847 115 21 4.312657754796916 115 22 4.78912293549325 115 23 4.9979208487486915 115 24 5.499121404651646 115 25 4.597390168595112 115 26 4.2921446952326185 115 27 4.2056693939891225 115 28 4.456049419536821 115 29 4.274467158883336 115 30 4.374133901098919 115 31 3.9317925746869697 115 32 4.182792132570107 115 33 4.10741580284396 115 34 3.9372559057500816 115 35 4.683670632498316 115 36 5.645481997244556 115 37 6.416226367302276 115 38 7.137905647264471 115 39 6.755625786764764 115 40 9.894628322121438 115 41 11.131905060070277 115 42 10.27792592629024 115 43 7.595246421623177 115 44 0.07644021824992464 115 45 8.03870222790028 115 46 4.155585912223138 115 47 -6.378654257758109 115 48 3.9284345628659523 115 49 -2.799797930177615 115 50 4.151505286579702 115 51 5.679221867238457 115 52 5.9745748443586315 115 53 6.3642756966967475 115 54 7.193037702019097 115 55 6.111108316245431 115 56 8.966212752772545 116 1 0.9018615496993121 116 2 1.6339724720023128 116 3 1.7810120614085463 116 4 1.9505360449298834 116 5 2.281787135876513 116 6 3.7220410560747155 116 7 2.328130705926391 116 8 3.3975022471649368 116 9 3.447060271230967 116 10 4.008184624971285 116 11 3.48266380480419 116 12 3.250683944589415 116 13 4.515958576828655 116 14 4.305169887161561 116 15 -4.0747192507365 116 16 4.191963456452001 116 17 6.289701632934598 116 18 6.438656885144045 116 19 6.253988711315415 116 20 5.38369380282116 116 21 6.379954751459877 116 22 6.458755860965997 116 23 6.330512617380506 116 24 7.078654218889689 116 25 6.273141002855404 116 26 4.713168054520718 116 27 4.5616204507188165 116 28 4.076182901183092 116 29 5.475801975346069 116 30 5.633866937806371 116 31 4.274951640315447 116 32 3.7762292849616585 116 33 4.60574728226554 116 34 3.9405929469069214 116 35 4.670878641397092 116 36 4.4157823309486695 116 37 5.498540049171016 116 38 5.778061375851788 116 39 7.712800425251967 116 40 9.181613861609758 116 41 7.6419161835408005 116 42 5.841885881796358 116 43 2.1758915829135006 116 44 0.8050275374935261 116 45 5.206666632794612 116 46 0.1817260679517254 116 47 -4.873092522496709 116 48 1.6586904026879772 116 49 -4.479998152647024 116 50 1.5541749195051757 116 51 5.237620087483172 116 52 5.1764658343475105 116 53 5.8509275320693845 116 54 5.8293002159234355 116 55 5.704545468636976 116 56 8.19313155143773 117 1 -0.04121245409574925 117 2 1.2399180760503867 117 3 1.2898672163737572 117 4 1.4929142865491087 117 5 2.0028497544723365 117 6 3.2381700883327795 117 7 1.3787315914418912 117 8 2.2845990213584884 117 9 1.8879204862847274 117 10 2.51577475779861 117 11 -3.5293539055057366 117 12 -13.85304787071735 117 13 -14.858804231520487 117 14 -5.222344344337493 117 15 -11.255617416454399 117 16 -6.718852482826936 117 17 0.009499229392538666 117 18 -3.7131457714024485 117 19 -0.5572067346048897 117 20 -0.26051872089800554 117 21 5.870467085425723 117 22 6.756668574438482 117 23 5.760936399796698 117 24 6.432860417498169 117 25 5.733292805511951 117 26 5.3915361759628375 117 27 5.438597047809271 117 28 5.082824267407002 117 29 5.895156814055749 117 30 6.189211656338944 117 31 4.196836523300899 117 32 4.974205249541036 117 33 5.449200792701973 117 34 5.567411416230133 117 35 5.068728103762684 117 36 6.374352855932859 117 37 6.946990397052108 117 38 7.0554174795836 117 39 8.724185624492957 117 40 10.999766929288445 117 41 9.198842179745736 117 42 8.744795432713499 117 43 -0.8263222058278039 117 44 0.4773868037806892 117 45 8.20097403317412 117 46 2.6833073456240832 117 47 -1.0473493219906977 117 48 2.164066746774001 117 49 -2.710548437277005 117 50 3.736021851826493 117 51 7.716747222208031 117 52 8.795061659774454 117 53 8.059339929286335 117 54 8.751185098908888 117 55 9.059384178314396 117 56 10.620709034043674 118 1 -0.00297715997810144 118 2 0.6541337623248964 118 3 0.828592706569836 118 4 1.2836005610589183 118 5 1.270496813295864 118 6 2.888170088332778 118 7 1.2571629639909077 118 8 1.4595990213584855 118 9 1.5962538196180631 118 10 2.0275394636809665 118 11 -2.8479813564861303 118 12 -11.113832184442842 118 13 -9.114686584461666 118 14 -12.214991403161022 118 15 -11.286009573317145 118 16 -11.705617188709287 118 17 -2.2498144960976596 118 18 -9.323439889049506 118 19 -1.8645596757813614 118 20 -2.1840481326627135 118 21 4.405761203072778 118 22 5.49343328032084 118 23 4.628093262541796 118 24 5.706880025341306 118 25 3.9215280996295974 118 26 4.418006764198136 118 27 3.4979107732994628 118 28 4.69311838505406 118 29 4.895156814055749 118 30 5.524995970064435 118 31 5.025758091928349 118 32 4.231067994639076 118 33 4.031553733878447 118 34 4.413980043681111 118 35 4.910394770429345 118 36 5.635137169658346 118 37 6.7670884362677945 118 38 7.060319440367916 118 39 8.116832683316485 118 40 9.417904184190409 118 41 9.16207747386338 118 42 9.71930523663507 118 43 2.420246421623176 118 44 2.0945436665257873 118 45 8.517150503762352 118 46 6.08575832601624 118 47 -0.4174473612063849 118 48 4.647400080107332 118 49 -2.8679013784534746 118 50 5.617884596924533 118 51 9.934394281031562 118 52 9.57888518918622 118 53 8.782379144972609 118 54 10.15510666753634 118 55 10.134384178314395 118 56 12.258454132082889 119 1 0.367022840021896 119 2 1.2191337623248941 119 3 1.2835927065698343 119 4 1.3086005610589169 119 5 1.760496813295866 119 6 2.4981700883327775 119 7 1.617162963990907 119 8 1.924599021358489 119 9 1.8412538196180606 119 10 2.467539463680964 119 11 -2.1379813564861294 119 12 -12.273832184442842 119 13 -5.924686584461664 119 14 -8.199991403161022 119 15 -11.216009573317145 119 16 -10.410617188709288 119 17 -2.394814496097659 119 18 -8.968439889049506 119 19 -1.7395596757813614 119 20 -2.304048132662711 119 21 4.605761203072781 119 22 5.128433280320838 119 23 3.8130932625417913 119 24 3.341880025341304 119 25 4.281528099629597 119 26 5.568006764198135 119 27 5.442910773299463 119 28 5.45811838505406 119 29 5.335156814055747 119 30 5.569995970064436 119 31 4.020758091928354 119 32 4.0310679946390735 119 33 4.2715537338784415 119 34 4.508980043681117 119 35 4.5553947704293485 119 36 4.875137169658348 119 37 5.292088436267793 119 38 4.130319440367916 119 39 6.701832683316486 119 40 8.482904184190406 119 41 6.447077473863381 119 42 4.5593052366350655 119 43 -1.2247535783768235 119 44 0.2095436665257857 119 45 4.082150503762353 119 46 1.8707583260162401 119 47 -1.2124473612063866 119 48 -2.4225999198926687 119 49 -5.572901378453476 119 50 1.8528845969245324 119 51 6.074394281031559 119 52 5.993885189186219 119 53 6.357379144972608 119 54 6.135106667536338 119 55 6.799384178314394 119 56 9.603454132082891 120 1 0.3021248808382211 120 2 0.7485215174269371 120 3 1.0729804616718752 120 4 1.4432944386099393 120 5 1.4266192622754588 120 6 2.952455802618495 120 7 1.0489996986847885 120 8 1.6315377968686917 120 9 1.9151313706384698 120 10 2.437743545313616 120 11 -3.2204303360779676 120 12 -10.589342388524475 120 13 -11.74836005384942 120 14 -9.23795058683449 120 15 -1.7462136549497984 120 16 -9.028576372382755 120 17 -1.1013451083425565 120 18 -9.193337848233181 120 19 -2.378335185985442 120 20 -1.7983338469484274 120 21 3.146577529603391 120 22 4.841902668075942 120 23 4.091868772745876 120 24 3.4308596171780366 120 25 3.0766301404459213 120 26 4.588414927463443 120 27 4.671890365136196 120 28 4.481383691176507 120 29 4.262503752831261 120 30 5.007138827207289 120 31 4.021166255193659 120 32 3.355557790557441 120 33 4.351961897143752 120 34 4.570612696742341 120 35 3.204782525531389 120 36 5.0494228839440645 120 37 5.24310884443106 120 38 4.8817480117964855 120 39 6.5561183976022015 120 40 6.557189898476121 120 41 -4.049657220014172 120 42 -3.123551906222076 120 43 -6.231284190621722 120 44 -15.640660415106867 120 45 -4.102747455421323 120 46 -9.383119225004167 120 47 -7.5067330754920985 120 48 -6.8087223688722585 120 49 -14.552085051922864 120 50 -5.964258260218326 120 51 -1.083973065907216 120 52 -1.7175433822423543 120 53 -0.4140494264559642 120 54 -0.9142810875657013 120 55 0.4675474436205178 120 56 3.156923519837992 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 131.0 5 64.0 6 482.0 7 259.0 8 200.0 9 543.0 10 211.0 11 309.0 12 298.0 13 162.0 14 192.0 15 178.0 16 226.0 17 277.0 18 380.0 19 507.0 20 605.0 21 763.0 22 1028.0 23 1221.0 24 1456.0 25 1616.0 26 1752.0 27 1899.0 28 2032.0 29 2186.0 30 2467.0 31 2783.0 32 3052.0 33 3399.0 34 3671.0 35 3980.0 36 4210.0 37 4823.0 38 4561.0 39 3565.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.4647491349481 0.38747116493656286 0.05947231833910035 0.08830738177623991 8 99.34760668973472 0.428921568627451 0.14777970011534025 0.07569204152249134 9 98.37980067403176 0.7623407284589183 0.26492691981905675 0.5929316776902698 10 68.37874855824683 20.22599480968858 4.876730103806228 6.518526528258361 11 43.4526384083045 22.941897347174166 18.097606689734718 15.50785755478662 12 35.96813725490196 15.13660611303345 22.754469434832757 26.140787197231834 13 26.594939446366784 15.509659746251442 26.11735870818916 31.778042099192618 14 22.34717416378316 18.771626297577853 30.446222606689734 28.43497693194925 15 26.394725402115636 18.734603680625998 28.40892624257354 26.46174467468483 16 34.51988098458209 19.808854025786673 22.793255792985303 22.87800919664593 17 31.05803893294881 21.00036049026676 25.675919250180247 22.26568132660418 18 30.00648788927336 21.051398500576703 24.807165513264128 24.134948096885815 19 29.639579539512827 25.16632710095018 21.574743522708832 23.619349836828153 20 31.159786374133947 24.171838914549653 23.86511258660508 20.803262124711317 21 29.79436624794997 23.738893794942957 24.515652314956657 21.951087642150412 22 25.49920703575548 21.810121107266436 28.912557670126876 23.77811418685121 23 28.982843137254903 21.34515570934256 26.5625 23.109501153402537 24 30.599113225911108 20.54359972603727 24.30337767203778 24.55390937601384 25 27.43755181487222 22.881447572360596 22.921097213711565 26.759903399055617 26 25.81244364292155 22.171325518485123 25.72407574391344 26.29215509467989 27 23.333273222368494 20.2640073575821 25.6577642327737 30.744955187275714 28 24.724264705882355 24.54584775086505 22.56523933102653 28.16464821222607 29 29.6613840655572 23.90166419984838 21.946861124147144 24.490090610447275 30 27.818748985737212 21.001099911646442 26.695396599289566 24.484754503326783 31 30.855052088965788 21.43397858765005 23.92487653653437 23.78609278684979 32 34.11868623138768 21.31809496340628 21.93640263907416 22.62681616613188 33 33.84093533487298 21.263351616628174 22.750072170900694 22.14564087759815 34 26.656555808327013 22.563463691185497 29.061495684830103 21.718484815657384 35 24.90884805602686 27.60730659542977 26.910580845456845 20.57326450308653 36 25.601774219721968 30.62332089215845 23.268603162582714 20.50630172553686 37 29.539177993177017 26.817205465605316 21.71440948719337 21.929207054024296 38 27.87871126200968 27.913024633388716 22.18088564617496 22.027378458426643 39 30.561019181839512 25.225111427901187 21.639569085298735 22.57430030496057 40 23.903883177739836 19.14163246031458 20.414835264630028 36.53964909731556 41 18.676135846307933 19.206045707001728 19.235181644359464 42.88263680233088 42 23.25875591860339 24.482235153793326 23.596703654172842 28.662305273430437 43 21.61815455218892 18.649610638336313 24.87939725730392 34.852837552170854 44 22.57369840207025 20.069129010658898 25.23751787039215 32.1196547168787 45 27.421335934395437 22.569632599978323 24.90516961092446 25.10386185470178 46 25.263424244067306 25.355600137360156 24.363353756619492 25.017621861953042 47 20.66411978780033 20.72386884177364 25.056580543535333 33.5554308268907 48 24.636635770187905 21.691852706747934 29.87340859090417 23.79810293215999 49 26.298196798323275 19.508184873342245 25.559209337621507 28.634408990712966 50 23.90091537725459 21.721703648870676 28.102262263708088 26.275118710166645 51 23.181777094820575 21.41733706951098 29.928590798156012 25.472295037512428 52 22.306470248411323 21.627671865973426 28.718948584633157 27.34690930098209 53 21.88228490883134 21.045593364295136 32.3544825342899 24.717639192583622 54 22.83242735629278 20.701424882162787 31.667057952431694 24.799089809112747 55 21.80678039612549 21.185123608500795 28.419112331935807 28.588983663437904 56 23.262157670351904 22.414914372425756 26.896813353566007 27.42611460365633 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 0.5 9 0.0 10 0.5 11 1.0 12 4.0 13 15.5 14 24.0 15 34.5 16 45.0 17 47.5 18 50.0 19 52.0 20 54.0 21 60.5 22 82.0 23 97.0 24 106.5 25 116.0 26 123.0 27 130.0 28 152.0 29 203.0 30 232.0 31 281.5 32 331.0 33 382.0 34 433.0 35 469.5 36 506.0 37 624.5 38 856.0 39 969.0 40 981.0 41 993.0 42 1093.5 43 1194.0 44 1293.0 45 1392.0 46 1558.0 47 1929.0 48 2134.0 49 2231.0 50 2328.0 51 2346.0 52 2364.0 53 2529.5 54 2943.0 55 3191.0 56 3311.5 57 3432.0 58 3694.5 59 3957.0 60 4216.5 61 4476.0 62 4805.0 63 5079.5 64 5025.0 65 4355.5 66 3686.0 67 3180.5 68 2675.0 69 2219.0 70 1763.0 71 1480.5 72 1044.0 73 890.0 74 696.5 75 503.0 76 448.5 77 394.0 78 299.0 79 149.0 80 94.0 81 71.0 82 48.0 83 28.0 84 8.0 85 5.5 86 3.0 87 2.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0018021914648212226 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5046136101499423 16 0.05947231833910035 17 0.01441753171856978 18 0.0 19 0.045054786620530564 20 0.11534025374855825 21 0.0018021914648212226 22 0.0 23 0.0 24 0.010813148788927337 25 0.003604382929642445 26 0.06848327566320646 27 0.06307670126874279 28 0.0 29 0.15498846597462515 30 0.05226355247981545 31 0.010813148788927337 32 0.025230680507497116 33 0.11534025374855825 34 0.1838235294117647 35 0.15498846597462515 36 0.048659169550173006 37 0.15679065743944637 38 0.2090542099192618 39 0.1279555940023068 40 0.7749423298731257 41 1.0326557093425606 42 0.2775374855824683 43 0.25410899653979235 44 0.41270184544405997 45 0.22707612456747406 46 0.2865484429065744 47 0.46316320645905423 48 0.061274509803921566 49 0.2559111880046136 50 0.18202133794694347 51 0.3117791234140715 52 0.17301038062283738 53 0.27213091118800464 54 0.20725201845444058 55 0.27393310265282583 56 0.2378892733564014 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 55488.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.11757497116494 #Duplication Level Percentage of deduplicated Percentage of total 1 88.72660138814237 68.42380334486737 2 6.926690191862775 10.683391003460208 3 1.9980837091911852 4.622621107266435 4 0.8389614638592228 2.5879469434832756 5 0.429996961977986 1.6580161476355246 6 0.22434624103199272 1.0380622837370241 7 0.17059662078474444 0.9209198385236447 8 0.11451006052674627 0.7064590542099193 9 0.08880372040849711 0.6163494809688581 >10 0.4533663620854853 6.10222029988466 >50 0.018695520085999395 0.8938869665513265 >100 0.009347760042999698 1.7463235294117647 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 413 0.7443050749711649 No Hit TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 260 0.46856978085351786 No Hit TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 156 0.28114186851211076 No Hit TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 140 0.25230680507497116 No Hit TCGGGTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 79 0.1423731257208766 No Hit TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 77 0.13876874279123416 No Hit TCGGGTGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCATCCTCTGCTCTTCC 66 0.1189446366782007 No Hit TCGGGTGGGGACGCTACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTC 59 0.10632929642445214 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0018021914648212226 0.0 0.0 0.0 0.0 11 0.0018021914648212226 0.0 0.0 0.0 0.0 12 0.003604382929642445 0.0 0.0 0.0 0.0 13 0.003604382929642445 0.0 0.0 0.0 0.0 14 0.003604382929642445 0.0 0.0 0.0 0.0 15 0.003604382929642445 0.0 0.0 0.0 0.005406574394463667 16 0.003604382929642445 0.0 0.0 0.0 0.005406574394463667 17 0.003604382929642445 0.0 0.0 0.0 0.005406574394463667 18 0.005406574394463667 0.0 0.0 0.0 0.005406574394463667 19 0.00720876585928489 0.0 0.0 0.0 0.005406574394463667 20 0.009010957324106113 0.0 0.0 0.0 0.005406574394463667 21 0.010813148788927335 0.0 0.0 0.0 0.005406574394463667 22 0.012615340253748558 0.0 0.0 0.0 0.005406574394463667 23 0.012615340253748558 0.0 0.0 0.0 0.005406574394463667 24 0.012615340253748558 0.0 0.0 0.0 0.005406574394463667 25 0.012615340253748558 0.0 0.0 0.0 0.005406574394463667 26 0.016219723183391002 0.0 0.0 0.0 0.005406574394463667 27 0.019824106113033448 0.0 0.0 0.0 0.005406574394463667 28 0.023428489042675894 0.0 0.0 0.0 0.005406574394463667 29 0.025230680507497116 0.0 0.0 0.0 0.005406574394463667 30 0.025230680507497116 0.0 0.0 0.0 0.005406574394463667 31 0.025230680507497116 0.0 0.0 0.0 0.005406574394463667 32 0.025230680507497116 0.0 0.0 0.0 0.005406574394463667 33 0.02703287197231834 0.0 0.0 0.0 0.005406574394463667 34 0.02703287197231834 0.0 0.0 0.0 0.005406574394463667 35 0.02703287197231834 0.0 0.0 0.0 0.005406574394463667 36 0.02703287197231834 0.0 0.0 0.0 0.005406574394463667 37 0.02883506343713956 0.0 0.0 0.0 0.005406574394463667 38 0.02883506343713956 0.0 0.0 0.0 0.005406574394463667 39 0.030637254901960783 0.0 0.0 0.0 0.005406574394463667 40 0.03424163783160323 0.0 0.0 0.0 0.005406574394463667 41 0.03424163783160323 0.0 0.0 0.0 0.005406574394463667 42 0.036043829296424454 0.0 0.0 0.0 0.005406574394463667 43 0.036043829296424454 0.0 0.0 0.0 0.005406574394463667 44 0.036043829296424454 0.0 0.0 0.0 0.005406574394463667 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATTATA 15 0.008046013 50.52979 41 TTCGTCA 15 0.008075243 50.483517 40 ACATGTG 15 0.008075243 50.483517 40 AACCCTT 20 3.9742474E-4 50.43733 37 ACCCTTG 20 3.9742474E-4 50.43733 38 GGCGCAT 15 0.0081045525 50.43733 37 TATAATT 15 0.0081045525 50.43733 38 AAATATA 20 4.0103245E-4 50.345207 35 AATATAA 20 4.0103245E-4 50.345207 36 TTCGCCC 20 4.0103245E-4 50.345207 35 TCGCCCC 15 0.008163407 50.345203 36 CCCGTGT 20 4.0284605E-4 50.29927 34 CAAATAT 25 1.9898192E-5 50.29927 34 ACTATTA 15 0.008192955 50.299267 47 ACCGCTC 15 0.00822258 50.253418 45 TTATACT 20 4.0832598E-4 50.161964 43 ATTATAC 30 1.0068907E-6 50.161964 42 GTCGGCG 15 0.008282071 50.161964 48 ATGGGAG 15 0.008402014 49.980053 30 TTGGCGC 15 0.008402014 49.980053 29 >>END_MODULE