FastQCFastQC Report
Fri 17 Jun 2016
SRR1526057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4133
Sequences flagged as poor quality0
Sequence length40
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC130.3145414952818776No Hit
TCGGGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT80.19356399709654004No Hit
TCGGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT80.19356399709654004No Hit
TCGGGTGGGAGAGTCGATTTTTTTTTTTTTTTTTTTTTTT60.14517299782240503No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG60.14517299782240503No Hit
TCGGGTGGGGTATCAGGTCGACCCTGATGCCTGCTTTTCG50.12097749818533754No Hit
TCGGGTGGGGTCGACTCTTTTTTTTTTTTTTTTTTTTTTT50.12097749818533754No Hit
TCGGGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT50.12097749818533754No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA50.12097749818533754No Hit
TCGGGTGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.12097749818533754No Hit
TCGGGTGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGAGC50.12097749818533754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGGGT251.6983891E-434.0000045
GTGGGGC251.6983891E-434.0000045
GTGGGCT251.6983891E-434.0000045
GGGAAGC251.6983891E-434.0000047
GGGAGCA200.002478746534.07
CGGGTGG3950.034.02
TGGGGGT200.002478746534.06
GTGGGGA405.2437827E-834.05
GTGGGAG453.4870027E-934.05
GTGGGAC405.2437827E-834.05
GTGGGAA357.8178346E-734.05
GGTGGGT301.1563492E-534.04
GGTGGGG1750.034.04
GGTGGGC301.1563492E-534.04
TGGGATC200.002478746534.06
TGGGAGC200.002478746534.06
TGGGAAG301.1563492E-534.06
GGGTGGG3750.034.03
TCGGGTG3950.034.01
GGTGGGA1450.032.8275834
Produced by FastQC (version 0.11.5)