FastQCFastQC Report
Fri 17 Jun 2016
SRR1526055_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526055_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6016
Sequences flagged as poor quality0
Sequence length40
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC1973.2746010638297873No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG510.8477393617021276No Hit
TCGGGTGGGACTAATCCTAGCCCTAGCCCTACACAAATAT220.36569148936170215No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA200.3324468085106383No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA160.26595744680851063No Hit
TCGGGTGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAGC120.19946808510638298No Hit
TCGGGTGGGGGGCTGGTGAGCTGGCTCAGTGGGTAAGAGC110.18284574468085107No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC100.16622340425531915No Hit
TCGGGTGGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAG100.16622340425531915No Hit
TCGGGTGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAG90.14960106382978725No Hit
TCGGGTGGGGATGGTGGTGGTGGTAGGGGTACGCTTAGGT90.14960106382978725No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC90.14960106382978725No Hit
TCGGGTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC90.14960106382978725No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGC80.13297872340425532No Hit
TCGGGTGGGATTCGTAGGAGTAAACATAACATTCTTCCCT80.13297872340425532No Hit
TCGGGTGGGATTTACAGTTGGTGGTCTAACCGGAATTGTT80.13297872340425532No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAG70.1163563829787234No Hit
TCGGGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT70.1163563829787234No Hit
TCGGGTGGGATCCAACTCATCCCTTGACATCGTGCTTCAC70.1163563829787234No Hit
TCGGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT70.1163563829787234No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGC70.1163563829787234No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG70.1163563829787234No Hit
TCGGGTGGGATGCCACAACTAGATACATCAACATGATTTA70.1163563829787234No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAG70.1163563829787234No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC70.1163563829787234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG1200.034.1092456
TGGGGAG251.76315E-434.1092456
GTGGGGG2050.034.1092455
GTGGGGA502.746674E-1034.1092455
GGTGGGT301.22458005E-534.1092454
TGGGGTA200.002532756734.109246
CGGGTGG5850.034.109242
TGGGGGC200.002532756734.109246
TGGGGGA405.851325E-834.109246
TGGGGCT200.002532756734.109246
GTGGGGT405.851325E-834.109245
GTGGGAC405.851325E-834.109245
GTGGGAA358.48062E-734.109245
TGGGACC200.002532756734.109246
TCGGGTG6000.033.8251
TGTGGGT200.00264071233.82499715
GGGTGGG5850.033.817713
GGTGGGG3350.033.600154
GGTGGGA1700.033.106034
GGTGGGC605.0931703E-1131.2668084
Produced by FastQC (version 0.11.5)