FastQCFastQC Report
Fri 17 Jun 2016
SRR1526054_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526054_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33777
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT1710.5062616573407941No Hit
TGAAGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC1500.44408917310595974No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1460.4322467951564674No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1000.29605944873730644No Hit
TGAAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.21020220860348757No Hit
TGAAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.15987210231814547No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC540.15987210231814547No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG500.14802972436865322No Hit
TGAAGCGGGGATCCCCTCGACCACTCTTTTGGCGCTTCGCTGTCGACAGTGCGCTT460.13618734641916097No Hit
TGAAGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA450.1332267519317879No Hit
TGAAGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT450.1332267519317879No Hit
TGAAGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC410.12138437398229564No Hit
TGAAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA370.10954199603280339No Hit
TGAAGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT360.10658140154543032No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC350.10362080705805726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT204.05637E-450.1716415
TTGAGAC150.00829658150.0968747
ACCTGGT150.00834566650.02232411
ATCCTGC150.00839496749.94799420
GTACCTG150.00839496749.9479949
TGATCCT150.00839496749.94799418
GATCCTG150.00839496749.94799419
GGCTTTT150.00839496749.9479949
CATATGC150.00839496749.94799433
TAGCATA150.00844448449.8738930
GCATATG150.00844448449.8738932
GGGTCCT150.00849421949.8000038
CGGGGTG900.049.8000036
CGGGGTC800.049.8000036
GGATCCT252.0933516E-549.8000038
GCCAGTA150.00849421949.80000325
CGGGCTA150.00849421949.8000036
CGGGCGT150.00849421949.8000036
CGGGTGC150.00849421949.8000036
CGGGTCC451.3278623E-1049.8000036
Produced by FastQC (version 0.11.5)