FastQCFastQC Report
Fri 17 Jun 2016
SRR1526053_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526053_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220
Sequences flagged as poor quality0
Sequence length40
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT100.45045045045045046No Hit
TGAAGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTA100.45045045045045046No Hit
TGAAGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTA100.45045045045045046No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT80.36036036036036034No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT60.2702702702702703No Hit
TGAAGCGGGAGATGCAGCCATTGAAGGAGCTTCAGATGAC50.22522522522522523No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATTTGCT50.22522522522522523No Hit
TGAAGCGGGGATGAGATGGACTTAAATGAATTGACGGGTA50.22522522522522523No Hit
TGAAGCGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT40.18018018018018017No Hit
TGAAGCGGGCTCGTCTTAATCAGCAACATCTTTTGATACA40.18018018018018017No Hit
TGAAGCGGGATGCCACAACTAGATACATCAACATGATTTA40.18018018018018017No Hit
TGAAGCGGGATGAGATGGACTTAAATGAATTGACGGGTAC40.18018018018018017No Hit
TGAAGCGGGGATGAGATGGACTCAAATGAATTGACGGGTA40.18018018018018017No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATCTGCT30.13513513513513514No Hit
TGAAGCGGGCTCGTCTTACTCAGCAACATCTTTTGATACA30.13513513513513514No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT30.13513513513513514No Hit
TGAAGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT30.13513513513513514No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCC30.13513513513513514No Hit
TGAAGCAGGGATGAGATGGACTTAAATGAATTGACGGGTA30.13513513513513514No Hit
TGAAGCGGGGATGAGATGGACTCAAAGGAATTGCCGGGTA30.13513513513513514No Hit
TGAAGCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCCGA30.13513513513513514No Hit
TGAAGCGGGAAGACTGTCTAATTCATTTTAAAGTGTGGAA30.13513513513513514No Hit
TGAAGCGGGATGAGATGGACTTAAAGGAATTGACGGGTAC30.13513513513513514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT309.203564E-634.0000046
CGGGAAG251.4457849E-434.0000046
ATGAGAT251.4457849E-434.00000411
GCGGGGG403.5090125E-834.05
GCGGGGA403.5090125E-834.05
GCGGGAT200.002229253834.05
CGGGGGG200.002229253834.06
AGCGGGG1050.034.04
TGAAGCG2200.033.2272721
GAAGCGG2150.033.20932
AAGCGGG2050.033.1707343
AGCGGGA750.031.7333344
GCGGGAA352.6495376E-529.1428575
GATGAGA304.22405E-428.33333610
GGGGATG304.22405E-428.3333367
GGGATGA406.6023415E-525.58
GGATGAG350.001042189524.2857139
TGAGATG350.001042189524.28571312
Produced by FastQC (version 0.11.5)