##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526053_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2220 Sequences flagged as poor quality 0 Sequence length 40 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.7990990990991 30.0 27.0 33.0 4.0 37.0 2 21.186936936936938 23.0 10.0 31.0 4.0 35.0 3 17.607207207207207 18.0 7.0 26.0 4.0 32.0 4 20.82702702702703 23.0 7.0 30.0 4.0 35.0 5 20.513513513513512 22.0 4.0 30.0 4.0 35.0 6 17.183783783783785 13.0 4.0 29.0 4.0 34.0 7 21.60945945945946 25.0 4.0 31.0 4.0 35.0 8 20.648198198198198 25.0 4.0 31.0 4.0 35.0 9 21.967117117117116 27.0 4.0 31.0 4.0 35.0 10 16.102702702702704 15.0 4.0 25.0 4.0 31.0 11 18.56261261261261 20.0 4.0 29.0 4.0 34.0 12 20.072522522522522 23.0 4.0 30.0 4.0 35.0 13 20.436486486486487 24.0 4.0 31.0 4.0 35.0 14 20.415315315315315 25.0 4.0 31.0 4.0 35.0 15 18.865765765765765 21.0 4.0 30.0 4.0 35.0 16 18.552702702702703 20.0 4.0 31.0 4.0 35.0 17 19.311711711711713 23.0 4.0 32.0 4.0 35.0 18 18.738288288288288 21.0 4.0 31.0 4.0 35.0 19 16.21936936936937 14.0 4.0 28.0 4.0 33.0 20 17.158558558558557 16.0 4.0 30.0 4.0 34.0 21 14.787387387387387 10.0 4.0 26.0 4.0 33.0 22 14.854954954954955 7.0 4.0 27.0 4.0 33.0 23 15.187387387387387 6.0 4.0 28.0 4.0 33.0 24 14.628378378378379 4.0 4.0 27.0 4.0 33.0 25 14.258108108108107 4.0 4.0 27.0 4.0 33.0 26 12.123423423423423 4.0 4.0 21.0 4.0 32.0 27 12.257207207207207 4.0 4.0 22.0 4.0 32.0 28 12.093693693693695 4.0 4.0 23.0 4.0 32.0 29 11.458108108108108 4.0 4.0 20.0 4.0 31.0 30 10.663963963963964 4.0 4.0 18.0 4.0 30.0 31 11.073873873873874 4.0 4.0 18.0 4.0 32.0 32 10.756306306306307 4.0 4.0 17.0 4.0 31.0 33 10.772522522522523 4.0 4.0 18.0 4.0 31.0 34 10.526576576576577 4.0 4.0 16.0 4.0 31.0 35 10.443243243243243 4.0 4.0 15.0 4.0 31.0 36 10.7490990990991 4.0 4.0 15.0 4.0 34.0 37 10.866666666666667 4.0 4.0 15.0 4.0 33.0 38 10.524324324324324 4.0 4.0 10.0 4.0 33.0 39 10.286036036036036 4.0 4.0 4.0 4.0 32.0 40 10.180630630630631 4.0 4.0 4.0 4.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -14.145760743321715 1 2 -15.48292682926829 1 3 -12.60752032520325 1 4 -14.963211382113823 1 5 -14.383536585365857 1 6 -11.358536585365853 1 7 -16.309146341463414 1 8 -15.09857723577236 1 9 -16.82682926829268 1 10 -11.495731707317074 1 11 -12.926219512195118 1 12 -14.7729674796748 1 13 -15.463617886178863 1 14 -15.566666666666674 1 15 -12.998170731707315 1 16 -13.618902439024389 1 17 -13.593089430894306 1 18 -13.034756097560976 1 19 -10.949593495934957 1 20 -11.58739837398374 1 21 -10.53841463414634 1 22 -9.865650406504065 1 23 -10.36869918699187 1 24 -10.169105691056911 1 25 -9.864024390243898 1 26 -7.9109756097561 1 27 -7.678861788617885 1 28 -7.490040650406504 1 29 -6.850203252032522 1 30 -6.293495934959353 1 31 -6.367073170731711 1 32 -6.175813008130083 1 33 -5.831504065040653 1 34 -5.768699186991869 1 35 -5.1089430894308965 1 36 -5.294918699186992 1 37 -5.620934959349594 1 38 -5.277439024390242 1 39 -4.955284552845528 1 40 -5.283333333333333 6 1 -11.374332171893144 6 2 -8.282926829268291 6 3 -5.407520325203251 6 4 -10.563211382113822 6 5 -7.3835365853658566 6 6 -7.358536585365853 6 7 -9.709146341463414 6 8 -11.09857723577236 6 9 -7.42682926829268 6 10 -5.895731707317074 6 11 -2.3262195121951184 6 12 -3.5729674796748014 6 13 -5.863617886178863 6 14 -4.5666666666666735 6 15 -4.798170731707316 6 16 -3.818902439024388 6 17 -2.7930894308943053 6 18 -1.4347560975609763 6 19 0.05040650406504277 6 20 -1.5873983739837403 6 21 0.8615853658536601 6 22 -2.4656504065040643 6 23 2.231300813008131 6 24 2.43089430894309 6 25 2.735975609756103 6 26 -1.1109756097560997 6 27 -2.478861788617886 6 28 -0.6900406504065035 6 29 -1.6502032520325223 6 30 0.506504065040648 6 31 0.03292682926828938 6 32 0.2241869918699173 6 33 -0.23150406504065302 6 34 0.8313008130081307 6 35 0.6910569105691042 6 36 -0.49491869918699116 6 37 -0.42093495934959435 6 38 -2.077439024390242 6 39 -0.755284552845529 6 40 0.5166666666666675 7 1 11.592334494773521 7 2 16.01707317073171 7 3 15.89247967479675 7 4 16.370121951219513 7 5 16.94979674796748 7 6 19.141463414634146 7 7 11.85752032520325 7 8 8.568089430894307 7 9 11.506504065040655 7 10 14.504268292682926 7 11 10.74044715447155 7 12 9.2270325203252 7 13 15.703048780487801 7 14 8.266666666666659 7 15 9.835162601626017 7 16 8.881097560975611 7 17 7.240243902439026 7 18 8.798577235772356 7 19 8.71707317073171 7 20 13.079268292682928 7 21 12.96158536585366 7 22 16.46768292682927 7 23 13.797967479674798 7 24 11.830894308943089 7 25 16.135975609756102 7 26 6.922357723577232 7 27 11.821138211382115 7 28 12.009959349593496 7 29 11.316463414634146 7 30 13.53983739837398 7 31 7.466260162601621 7 32 8.657520325203249 7 33 7.5018292682926795 7 34 4.897967479674797 7 35 7.557723577235771 7 36 7.53841463414634 7 37 6.045731707317072 7 38 6.055894308943092 7 39 4.044715447154472 7 40 7.049999999999999 9 1 5.5923344947735245 9 2 9.850406504065042 9 3 5.39247967479675 9 4 9.203455284552845 9 5 9.783130081300811 9 6 1.1414634146341474 9 7 9.524186991869918 9 8 10.234756097560972 9 9 8.506504065040652 9 10 8.504268292682926 9 11 9.74044715447155 9 12 12.393699186991867 9 13 8.203048780487805 9 14 4.599999999999994 9 15 13.168495934959353 9 16 4.381097560975611 9 17 9.740243902439026 9 18 14.965243902439024 9 19 6.883739837398375 9 20 14.41260162601626 9 21 16.794918699186994 9 22 5.301016260162603 9 23 11.964634146341462 9 24 14.16422764227642 9 25 13.135975609756102 9 26 10.922357723577232 9 27 11.987804878048783 9 28 8.843292682926828 9 29 7.983130081300811 9 30 12.206504065040647 9 31 10.299593495934957 9 32 10.490853658536585 9 33 -0.1648373983739866 9 34 8.064634146341463 9 35 9.391056910569104 9 36 9.871747967479676 9 37 13.879065040650406 9 38 4.722560975609758 9 39 5.711382113821138 9 40 2.883333333333333 12 1 4.425667828106857 12 2 10.51707317073171 12 3 -5.60752032520325 12 4 0.03678861788617738 12 5 12.616463414634143 12 6 -3.3585365853658526 12 7 5.690853658536586 12 8 9.90142276422764 12 9 2.1731707317073194 12 10 7.5042682926829265 12 11 4.073780487804882 12 12 14.2270325203252 12 13 8.536382113821137 12 14 8.433333333333326 12 15 16.001829268292685 12 16 13.381097560975611 12 17 17.406910569105694 12 18 17.965243902439024 12 19 9.050406504065043 12 20 8.41260162601626 12 21 20.461585365853658 12 22 21.134349593495934 12 23 18.63130081300813 12 24 18.830894308943087 12 25 19.135975609756102 12 26 23.0890243902439 12 27 6.321138211382115 12 28 23.509959349593494 12 29 14.149796747967478 12 30 24.706504065040647 12 31 22.63292682926829 12 32 16.824186991869915 12 33 19.168495934959346 12 34 25.231300813008133 12 35 -0.10894308943089648 12 36 20.705081300813006 12 37 17.379065040650406 12 38 23.72256097560976 12 39 18.044715447154474 12 40 23.71666666666667 13 1 -11.774332171893143 13 2 -12.48292682926829 13 3 -10.00752032520325 13 4 -13.163211382113822 13 5 -12.583536585365856 13 6 -2.758536585365853 13 7 -9.909146341463414 13 8 -10.09857723577236 13 9 -12.02682926829268 13 10 -8.695731707317073 13 11 -3.7262195121951187 13 12 -4.3729674796748 13 13 -4.0636178861788625 13 14 -4.766666666666673 13 15 -4.598170731707315 13 16 -4.218902439024388 13 17 -4.993089430894306 13 18 -2.2347560975609753 13 19 -0.14959349593495652 13 20 -1.9873983739837406 13 21 -0.7384146341463396 13 22 -3.8656504065040647 13 23 -4.36869918699187 13 24 -4.769105691056911 13 25 -6.064024390243898 13 26 -4.9109756097561 13 27 -1.8788617886178844 13 28 -7.490040650406504 13 29 -6.850203252032522 13 30 -6.293495934959353 13 31 -6.367073170731711 13 32 -6.175813008130083 13 33 -5.831504065040653 13 34 -5.768699186991869 13 35 -5.1089430894308965 13 36 -5.294918699186992 13 37 -5.620934959349594 13 38 -5.277439024390242 13 39 -4.955284552845528 13 40 -5.283333333333333 15 1 5.925667828106857 15 2 6.5170731707317096 15 3 6.89247967479675 15 4 3.0367886178861774 15 5 7.6164634146341434 15 6 11.141463414634147 15 7 14.190853658536586 15 8 14.40142276422764 15 9 12.67317073170732 15 10 17.004268292682926 15 11 15.073780487804882 15 12 6.727032520325199 15 13 12.536382113821137 15 14 14.433333333333326 15 15 9.501829268292685 15 16 16.88109756097561 15 17 13.906910569105694 15 18 8.965243902439024 15 19 6.550406504065043 15 20 13.41260162601626 15 21 17.961585365853658 15 22 -1.3656504065040647 15 23 14.13130081300813 15 24 4.330894308943089 15 25 -9.864024390243898 15 26 -7.9109756097561 15 27 -7.678861788617885 15 28 -7.490040650406504 15 29 -6.850203252032522 15 30 -6.293495934959353 15 31 -6.367073170731711 15 32 -6.175813008130083 15 33 -5.831504065040653 15 34 -5.768699186991869 15 35 -5.1089430894308965 15 36 -5.294918699186992 15 37 -5.620934959349594 15 38 -5.277439024390242 15 39 -4.955284552845528 15 40 -5.283333333333333 16 1 5.259001161440189 16 2 8.850406504065042 16 3 6.225813008130082 16 4 10.87012195121951 16 5 8.783130081300811 16 6 4.4747967479674795 16 7 9.690853658536586 16 8 11.40142276422764 16 9 7.6731707317073194 16 10 9.837601626016259 16 11 9.907113821138214 16 12 7.893699186991867 16 13 6.869715447154469 16 14 14.433333333333326 16 15 6.668495934959353 16 16 16.88109756097561 16 17 16.906910569105694 16 18 16.965243902439024 16 19 19.88373983739838 16 20 14.91260162601626 16 21 7.628252032520328 16 22 -0.1989837398373986 16 23 12.63130081300813 16 24 5.164227642276421 16 25 5.969308943089434 16 26 4.5890243902439 16 27 8.154471544715447 16 28 1.1766260162601618 16 29 -0.6835365853658555 16 30 3.2065040650406473 16 31 3.466260162601623 16 32 3.490853658536583 16 33 3.1684959349593473 16 34 5.564634146341465 16 35 8.891056910569104 16 36 0.7050813008130081 16 37 -0.7876016260162597 16 38 -1.444105691056909 16 39 -0.12195121951219434 16 40 -1.2833333333333332 17 1 2.4256678281068567 17 2 0.3504065040650417 17 3 1.3924796747967498 17 4 1.8701219512195095 17 5 1.4497967479674756 17 6 9.808130081300815 17 7 3.024186991869918 17 8 2.5680894308943074 17 9 2.5065040650406516 17 10 3.8376016260162586 17 11 0.40711382113821415 17 12 4.393699186991867 17 13 2.036382113821137 17 14 2.5999999999999943 17 15 2.501829268292685 17 16 -1.4522357723577208 17 17 -0.09308943089430599 17 18 1.465243902439024 17 19 4.717073170731711 17 20 3.0792682926829276 17 21 3.4615853658536597 17 22 5.634349593495935 17 23 6.7979674796747975 17 24 5.664227642276421 17 25 3.135975609756102 17 26 6.5890243902439 17 27 8.487804878048783 17 28 7.009959349593496 17 29 8.48313008130081 17 30 1.7065040650406473 17 31 8.299593495934957 17 32 7.490853658536585 17 33 7.168495934959347 17 34 8.231300813008131 17 35 10.224390243902436 17 36 5.538414634146342 17 37 4.545731707317072 17 38 5.222560975609758 17 39 4.711382113821138 17 40 4.716666666666667 18 1 9.259001161440192 18 2 12.850406504065045 18 3 0.725813008130082 18 4 14.203455284552842 18 5 15.44979674796748 18 6 10.141463414634147 18 7 13.35752032520325 18 8 13.40142276422764 18 9 13.839837398373984 18 10 13.337601626016259 18 11 10.573780487804882 18 12 9.060365853658531 18 13 14.203048780487801 18 14 12.099999999999994 18 15 14.001829268292685 18 16 6.881097560975611 18 17 15.74024390243903 18 18 17.13191056910569 18 19 17.050406504065045 18 20 5.579268292682928 18 21 13.628252032520328 18 22 16.46768292682927 18 23 17.297967479674796 18 24 11.497560975609757 18 25 9.30264227642277 18 26 4.5890243902439 18 27 13.487804878048783 18 28 16.34329268292683 18 29 -1.5168699186991876 18 30 3.2065040650406473 18 31 -1.533739837398377 18 32 -1.8424796747967491 18 33 -1.4981707317073187 18 34 -1.9353658536585359 18 35 0.22439024390243745 18 36 -1.2949186991869919 18 37 -1.6209349593495936 18 38 -2.444105691056909 18 39 -0.9552845528455283 18 40 -1.1166666666666671 19 1 6.425667828106857 19 2 4.850406504065042 19 3 4.39247967479675 19 4 4.036788617886177 19 5 8.283130081300811 19 6 10.97479674796748 19 7 11.024186991869918 19 8 7.90142276422764 19 9 9.17317073170732 19 10 12.170934959349594 19 11 10.407113821138214 19 12 16.893699186991864 19 13 6.203048780487805 19 14 6.099999999999994 19 15 6.001829268292685 19 16 7.381097560975611 19 17 5.073577235772362 19 18 6.965243902439024 19 19 2.383739837398375 19 20 5.745934959349592 19 21 5.128252032520328 19 22 4.801016260162603 19 23 -2.0353658536585364 19 24 4.4975609756097565 19 25 5.80264227642277 19 26 -5.577642276422767 19 27 0.9878048780487809 19 28 -7.490040650406504 19 29 -6.850203252032522 19 30 -6.293495934959353 19 31 -6.367073170731711 19 32 -6.175813008130083 19 33 -5.831504065040653 19 34 -5.768699186991869 19 35 -5.1089430894308965 19 36 -5.294918699186992 19 37 -5.620934959349594 19 38 -5.277439024390242 19 39 -4.955284552845528 19 40 -5.283333333333333 25 1 7.425667828106857 25 2 5.5170731707317096 25 3 2.64247967479675 25 4 6.786788617886177 25 5 5.3664634146341434 25 6 13.141463414634147 25 7 4.940853658536586 25 8 10.15142276422764 25 9 8.42317073170732 25 10 10.504268292682926 25 11 12.323780487804882 25 12 9.2270325203252 25 13 12.536382113821137 25 14 12.683333333333326 25 15 14.751829268292685 25 16 11.881097560975611 25 17 10.156910569105694 25 18 7.215243902439024 25 19 7.800406504065043 25 20 6.91260162601626 25 21 1.4615853658536597 25 22 8.384349593495935 25 23 5.38130081300813 25 24 3.0808943089430887 25 25 -9.864024390243898 25 26 -7.9109756097561 25 27 -7.678861788617885 25 28 -7.490040650406504 25 29 -6.850203252032522 25 30 -6.293495934959353 25 31 -6.367073170731711 25 32 -6.175813008130083 25 33 -5.831504065040653 25 34 -5.768699186991869 25 35 -5.1089430894308965 25 36 -5.294918699186992 25 37 -5.620934959349594 25 38 -5.277439024390242 25 39 -4.955284552845528 25 40 -5.283333333333333 26 1 6.6256678281068595 26 2 4.117073170731711 26 3 -3.60752032520325 26 4 5.636788617886179 26 5 5.816463414634143 26 6 9.441463414634148 26 7 0.8908536585365852 26 8 1.7014227642276403 26 9 0.573170731707318 26 10 -1.6957317073170728 26 11 1.4737804878048806 26 12 0.027032520325199982 26 13 1.1363821138211385 26 14 0.6333333333333258 26 15 3.201829268292684 26 16 -2.018902439024389 26 17 1.2069105691056947 26 18 3.5652439024390254 26 19 -1.9495934959349572 26 20 4.21260162601626 26 21 -1.338414634146341 26 22 1.3343495934959346 26 23 -5.168699186991871 26 24 -5.369105691056911 26 25 -5.0640243902438975 26 26 -1.1109756097560997 26 27 -0.8788617886178844 26 28 -0.6900406504065035 26 29 -0.05020325203252085 26 30 -6.293495934959353 26 31 -6.367073170731711 26 32 -6.175813008130083 26 33 -5.831504065040653 26 34 -5.768699186991869 26 35 -5.1089430894308965 26 36 -5.294918699186992 26 37 -5.620934959349594 26 38 -5.277439024390242 26 39 -4.955284552845528 26 40 -5.283333333333333 27 1 7.6256678281068595 27 2 9.717073170731709 27 3 4.1924796747967505 27 4 8.436788617886176 27 5 11.416463414634144 27 6 8.241463414634149 27 7 5.690853658536586 27 8 7.301422764227638 27 9 5.1731707317073194 27 10 5.904268292682925 27 11 1.4737804878048806 27 12 6.0270325203252 27 13 3.7363821138211364 27 14 0.033333333333327886 27 15 -4.598170731707315 27 16 0.5810975609756106 27 17 3.406910569105694 27 18 -0.8347560975609767 27 19 -0.3495934959349576 27 20 -4.987398373983741 27 21 -5.13841463414634 27 22 -0.265650406504065 27 23 -4.96869918699187 27 24 -10.169105691056911 27 25 -9.864024390243898 27 26 -7.9109756097561 27 27 -7.678861788617885 27 28 -7.490040650406504 27 29 -6.850203252032522 27 30 -6.293495934959353 27 31 -6.367073170731711 27 32 -6.175813008130083 27 33 -5.831504065040653 27 34 -5.768699186991869 27 35 -5.1089430894308965 27 36 -5.294918699186992 27 37 -5.620934959349594 27 38 -5.277439024390242 27 39 -4.955284552845528 27 40 -5.283333333333333 32 1 -12.907665505226477 32 2 -7.816260162601624 32 3 -6.940853658536584 32 4 -6.2965447154471565 32 5 -5.7168699186991905 32 6 -9.025203252032519 32 7 -11.642479674796748 32 8 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15.690853658536586 120 8 10.90142276422764 120 9 15.17317073170732 120 10 20.504268292682926 120 11 21.073780487804882 120 12 7.227032520325199 120 13 18.536382113821137 120 14 18.433333333333326 120 15 21.001829268292685 120 16 14.381097560975611 120 17 -3.593089430894306 120 18 14.965243902439024 120 19 -1.9495934959349572 120 20 9.41260162601626 120 21 15.46158536585366 120 22 13.134349593495935 120 23 6.63130081300813 120 24 4.830894308943089 120 25 12.135975609756102 120 26 17.0890243902439 120 27 3.321138211382115 120 28 4.509959349593496 120 29 20.14979674796748 120 30 -6.293495934959353 120 31 -6.367073170731711 120 32 -6.175813008130083 120 33 -5.831504065040653 120 34 -5.768699186991869 120 35 -5.1089430894308965 120 36 -5.294918699186992 120 37 -5.620934959349594 120 38 -5.277439024390242 120 39 -4.955284552845528 120 40 -5.283333333333333 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 536.0 5 10.0 6 29.0 7 19.0 8 23.0 9 21.0 10 43.0 11 52.0 12 76.0 13 95.0 14 80.0 15 82.0 16 91.0 17 79.0 18 105.0 19 65.0 20 71.0 21 87.0 22 108.0 23 101.0 24 95.0 25 87.0 26 64.0 27 58.0 28 51.0 29 41.0 30 16.0 31 18.0 32 11.0 33 3.0 34 2.0 35 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 97.47406405051872 2.345511953089761 0.04510599909788002 0.13531799729364005 8 97.79080252479712 0.7213706041478809 0.49594229035166815 0.9918845807033363 9 97.96747967479675 1.084010840108401 0.6323396567299007 0.31616982836495033 10 41.91077061739522 32.447048219918884 14.420910319963948 11.221270842721946 11 25.855855855855857 40.090090090090094 19.54954954954955 14.504504504504503 12 34.06940063091483 14.465975664713834 25.68724650743578 25.777377196935557 13 32.207207207207205 19.684684684684683 20.225225225225223 27.882882882882882 14 20.135135135135133 29.72972972972973 30.585585585585584 19.54954954954955 15 35.85585585585586 22.74774774774775 22.387387387387385 19.00900900900901 16 29.72972972972973 23.513513513513516 25.09009009009009 21.666666666666668 17 24.27927927927928 19.324324324324323 34.81981981981982 21.576576576576574 18 31.98198198198198 21.126126126126128 24.234234234234233 22.657657657657655 19 26.681715575620768 16.388261851015802 14.89841986455982 42.03160270880361 20 21.08108108108108 34.86486486486486 20.09009009009009 23.963963963963963 21 20.405405405405403 17.56756756756757 29.72972972972973 32.2972972972973 22 19.738621000450653 19.15277151870212 29.202343397926995 31.90626408292023 23 22.56756756756757 19.81981981981982 26.576576576576578 31.036036036036034 24 18.1981981981982 26.62162162162162 28.64864864864865 26.53153153153153 25 26.375112714156902 28.809738503155994 22.587917042380525 22.227231740306582 26 17.225747960108794 24.524025385312783 27.515865820489573 30.734360834088847 27 22.162162162162165 15.81081081081081 31.306306306306308 30.720720720720724 28 21.44144144144144 19.954954954954953 30.720720720720724 27.882882882882882 29 20.24346257889991 23.57980162308386 30.387736699729484 25.788999098286748 30 15.130984643179765 21.40921409214092 38.30171635049684 25.158084914182478 31 14.350180505415164 21.48014440433213 43.95306859205776 20.216606498194945 32 22.80306444344299 13.835060838215412 42.226228030644435 21.13564668769716 33 21.846846846846844 14.45945945945946 37.07207207207207 26.62162162162162 34 11.801801801801801 22.027027027027028 41.8018018018018 24.36936936936937 35 14.864864864864865 14.90990990990991 45.13513513513514 25.09009009009009 36 20.0 18.1981981981982 39.95495495495496 21.846846846846844 37 18.161333934204595 15.953132041460115 46.282109058134296 19.60342496620099 38 23.423423423423422 15.405405405405407 40.67567567567568 20.495495495495494 39 13.82882882882883 17.92792792792793 43.468468468468465 24.774774774774773 40 12.432432432432433 22.61261261261261 42.52252252252252 22.432432432432435 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 2.5 17 5.0 18 5.0 19 6.0 20 7.0 21 6.5 22 6.0 23 6.0 24 12.5 25 19.0 26 21.0 27 23.0 28 23.0 29 31.5 30 40.0 31 50.5 32 61.0 33 61.0 34 62.5 35 64.0 36 83.0 37 102.0 38 102.0 39 115.5 40 129.0 41 126.5 42 124.0 43 124.0 44 146.0 45 168.0 46 166.5 47 165.0 48 165.0 49 159.0 50 153.0 51 171.5 52 190.0 53 190.0 54 175.5 55 161.0 56 176.5 57 192.0 58 192.0 59 167.0 60 142.0 61 140.5 62 139.0 63 139.0 64 124.0 65 109.0 66 94.0 67 79.0 68 79.0 69 65.5 70 52.0 71 47.5 72 43.0 73 43.0 74 36.5 75 30.0 76 19.5 77 9.0 78 9.0 79 7.0 80 5.0 81 3.5 82 2.0 83 2.0 84 1.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.13513513513513514 8 0.09009009009009009 9 0.2702702702702703 10 0.04504504504504504 11 0.0 12 0.04504504504504504 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.22522522522522523 20 0.0 21 0.0 22 0.04504504504504504 23 0.0 24 0.0 25 0.09009009009009009 26 0.6306306306306306 27 0.0 28 0.0 29 0.09009009009009009 30 0.2702702702702703 31 0.18018018018018017 32 0.04504504504504504 33 0.0 34 0.0 35 0.0 36 0.0 37 0.04504504504504504 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 2220.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.73873873873873 #Duplication Level Percentage of deduplicated Percentage of total 1 96.34790965881788 90.31531531531532 2 2.5468524747717445 4.774774774774775 3 0.4805382027871216 1.3513513513513513 4 0.2402691013935608 0.9009009009009009 5 0.14416146083613646 0.6756756756756757 6 0.04805382027871216 0.2702702702702703 7 0.0 0.0 8 0.04805382027871216 0.36036036036036034 9 0.0 0.0 >10 0.14416146083613646 1.3513513513513513 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 10 0.45045045045045046 No Hit TGAAGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTA 10 0.45045045045045046 No Hit TGAAGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTA 10 0.45045045045045046 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT 8 0.36036036036036034 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT 6 0.2702702702702703 No Hit TGAAGCGGGAGATGCAGCCATTGAAGGAGCTTCAGATGAC 5 0.22522522522522523 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATTTGCT 5 0.22522522522522523 No Hit TGAAGCGGGGATGAGATGGACTTAAATGAATTGACGGGTA 5 0.22522522522522523 No Hit TGAAGCGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT 4 0.18018018018018017 No Hit TGAAGCGGGCTCGTCTTAATCAGCAACATCTTTTGATACA 4 0.18018018018018017 No Hit TGAAGCGGGATGCCACAACTAGATACATCAACATGATTTA 4 0.18018018018018017 No Hit TGAAGCGGGATGAGATGGACTTAAATGAATTGACGGGTAC 4 0.18018018018018017 No Hit TGAAGCGGGGATGAGATGGACTCAAATGAATTGACGGGTA 4 0.18018018018018017 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATCTGCT 3 0.13513513513513514 No Hit TGAAGCGGGCTCGTCTTACTCAGCAACATCTTTTGATACA 3 0.13513513513513514 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT 3 0.13513513513513514 No Hit TGAAGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.13513513513513514 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCC 3 0.13513513513513514 No Hit TGAAGCAGGGATGAGATGGACTTAAATGAATTGACGGGTA 3 0.13513513513513514 No Hit TGAAGCGGGGATGAGATGGACTCAAAGGAATTGCCGGGTA 3 0.13513513513513514 No Hit TGAAGCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCCGA 3 0.13513513513513514 No Hit TGAAGCGGGAAGACTGTCTAATTCATTTTAAAGTGTGGAA 3 0.13513513513513514 No Hit TGAAGCGGGATGAGATGGACTTAAAGGAATTGACGGGTAC 3 0.13513513513513514 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.09009009009009009 0.0 0.0 0.0 0.0 16 0.09009009009009009 0.0 0.0 0.0 0.0 17 0.09009009009009009 0.0 0.0 0.0 0.0 18 0.09009009009009009 0.0 0.0 0.0 0.0 19 0.09009009009009009 0.0 0.0 0.0 0.0 20 0.13513513513513514 0.0 0.0 0.0 0.0 21 0.13513513513513514 0.0 0.0 0.0 0.0 22 0.13513513513513514 0.0 0.0 0.0 0.0 23 0.13513513513513514 0.0 0.0 0.0 0.0 24 0.13513513513513514 0.0 0.0 0.0 0.0 25 0.13513513513513514 0.0 0.0 0.0 0.0 26 0.13513513513513514 0.0 0.0 0.0 0.0 27 0.13513513513513514 0.0 0.0 0.0 0.0 28 0.13513513513513514 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGGAT 30 9.203564E-6 34.000004 6 CGGGAAG 25 1.4457849E-4 34.000004 6 ATGAGAT 25 1.4457849E-4 34.000004 11 GCGGGGG 40 3.5090125E-8 34.0 5 GCGGGGA 40 3.5090125E-8 34.0 5 GCGGGAT 20 0.0022292538 34.0 5 CGGGGGG 20 0.0022292538 34.0 6 AGCGGGG 105 0.0 34.0 4 TGAAGCG 220 0.0 33.227272 1 GAAGCGG 215 0.0 33.2093 2 AAGCGGG 205 0.0 33.170734 3 AGCGGGA 75 0.0 31.733334 4 GCGGGAA 35 2.6495376E-5 29.142857 5 GATGAGA 30 4.22405E-4 28.333336 10 GGGGATG 30 4.22405E-4 28.333336 7 GGGATGA 40 6.6023415E-5 25.5 8 GGATGAG 35 0.0010421895 24.285713 9 TGAGATG 35 0.0010421895 24.285713 12 >>END_MODULE