FastQCFastQC Report
Fri 17 Jun 2016
SRR1526052_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526052_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT21.2987012987012987No Hit
TGAAGCGGGTGTCACCCCAGCGCCGCCCTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGATCATGTCTGGTGGCCCCCCGGATTACAACCG10.6493506493506493No Hit
TGAAGCGGGGTACGAAAAAAAAAAACAAAACACCCAACAC10.6493506493506493No Hit
TGAAGCCGGCCCCGAGGACTTTTTTTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCAGGCCTTCTGTATTTTCCGACACTTGCCCCGCCT10.6493506493506493No Hit
TGAAGCGGGATTCTTAAAGGGCCCCCAGAGACCGCACGGC10.6493506493506493No Hit
TGAAGCGGGATCTTGAATCGGGCGTGGTGGTCCCCCCCTT10.6493506493506493No Hit
TGAAGCGGGAAATGGTATCCCCCGAGCCCACCTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGCGTGGTGGGTTTTTCCCCTACTTATTTTTTG10.6493506493506493No Hit
TGAAGCGGGCCCGCAGAGCGATTTTTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGCATGGTACGCAGAGCGCTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGCTGCTTGTCCCAAAGATTCAGCCATCCATGT10.6493506493506493No Hit
TGAAGCGGGTGTTCAGTACAAACAGCAAACTCTCACACGC10.6493506493506493No Hit
TGAAGCGGGTGTATCACAGAGCCCCACCACAACCCAACAA10.6493506493506493No Hit
TGAAGCGGGGGGGCTGGACAGATGGCTCCGCCGTTAAGAG10.6493506493506493No Hit
TGAAGCGGGATGCTTGTCTCAAAGATTCAGCCATCCCTGT10.6493506493506493No Hit
TGAAGCGGGACCTACCCAGAGCCGACTTTTTTTTTTTTTC10.6493506493506493No Hit
TGAAGCGGGAGTCGACTTTTTTTTTTTTTTTTTTAAAGGT10.6493506493506493No Hit
TGAAGCGGGCTGTCCCAAACCATTTTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGCTCATGGCGGCCCCCCGAGCTAAGGCGCTTC10.6493506493506493No Hit
TGAAGCGGGCAGAGTTAACCCACACCCTCTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.6493506493506493No Hit
TGAAGCGGGAAGCAGTGTATCAACCCACAGTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGAACGCAGAGTTCAACGCAGAGTCGCCTTTTTC10.6493506493506493No Hit
TGAAGCGGGCGCAGAGTCGACTTTTTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCCTTAGCCTCCAAACGATCCGCCAAAGAGGACTAT10.6493506493506493No Hit
TGAAGCGGGATGGCTCCACGCAGAGTCGACTCTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGACTTATAAAAGCCTCTGTTTTCACTCTTGTC10.6493506493506493No Hit
TGAAGCGGGAGTACAGAGTCCTTTTTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGCAGGCCATCATTACAACATTAAAAACCTCTT10.6493506493506493No Hit
TGAAGCGGGAAGCTGGTCAACGCAGCGTCCTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGGGGGAAAAAAAAAAAAACAAAAAACCCAAAA10.6493506493506493No Hit
TGAAGCGGGACCCATGGTACAACCCCGAGTCCCCTTTTTT10.6493506493506493No Hit
TGAAGCGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGAAAAAAAAACAAAAAAAAAAAAAAACAAAAA10.6493506493506493No Hit
TGAAGCGGGGGGCTGGAGCGATGGCTCAGGGGTTACGAGC10.6493506493506493No Hit
TGAAGCGGGAGTCTGGTCCTTGTTCCCATCTTGTGTCTGC10.6493506493506493No Hit
TGAAGCGGGGAGAAGGCCGGGGACCCCGGCTACGTCTTGC10.6493506493506493No Hit
TGAAGCGGGATACAGTGCTTGAAGCCAACCAAAACAACAC10.6493506493506493No Hit
TGAAGCGGGGTTCTTCCGGTGGCGGATCGGTGGTTTACCC10.6493506493506493No Hit
TGAAGCGGGATGTTGATATCAGCATAGTGCTTTGGCTTGA10.6493506493506493No Hit
TGAAGCGGGCTCCATCTTTTTTTTTTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGAGTACAGCGTCCTTTTTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGTACCTGGTTGATCCTCCCAGACGCCTATCCT10.6493506493506493No Hit
TGAAGCGGGGAGATTCTTGACGTTTCCACCGCCGGTCCGC10.6493506493506493No Hit
TGAAGCGGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGAAGCAGTGGTATCAACCCACCTGGTCTCTTG10.6493506493506493No Hit
TGAAGCGGGCAGAGTCGCCTTTTTTTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGGGAACTCTGACCCCTGGCTGGAGGGATCGCT10.6493506493506493No Hit
TGAAGCGGGAGCTGGGAACCCCCCCTACCTGATCCTGGCT10.6493506493506493No Hit
TGAAGCGGGGGGGGCCGGTGAGCTGGCTCCGCGGGTAAGC10.6493506493506493No Hit
TGAAGCGGGGATGGCCCCCTGGTTAAGACCCCCCCCTGCT10.6493506493506493No Hit
TGAAGCGGGATCAAACTTTCCCCTAAACTCCACACCAACC10.6493506493506493No Hit
TGAAGCGGGAAAGTTATCCACCCCGCGTCGCCTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGGGCTTCCCGGAAGGGTTATGGTGCCAGTCCT10.6493506493506493No Hit
TGAAGCGGGGTTTATGTTCTCTGTTTCGCCCCCCGCCGGC10.6493506493506493No Hit
TGAAGCGGGTCCCTGGTTGATCCCGCCAGTACCATATGCT10.6493506493506493No Hit
TGAAGCGGGAGACGGTAGGATTTAACCGGACGGAGGCCAG10.6493506493506493No Hit
TGAAGCGGGGTTTATGTTCTCTGTTTAGGCCCCCGCCGGC10.6493506493506493No Hit
TGAAGCGGGTACCTGGTTGATCCTCCCAGTCCCCTATGCT10.6493506493506493No Hit
TGAAGCGGGAGGAAGCCCCGAAGCCACCTGGCTTTGCCCG10.6493506493506493No Hit
TGAAGCGGGGACTTTCTTGGCAAGATCCCGCCTTCTCCTT10.6493506493506493No Hit
TGAAGCGGGACTGCTGAATATAATGACTCCCATTGTAGTC10.6493506493506493No Hit
TGAAGCCCGTCGATCAACACCACTACCAACATATTAAACT10.6493506493506493No Hit
TGAAGCGGGGAGTACCTCCCCCCCCCCGGCTCCCTCGCCC10.6493506493506493No Hit
TGAAGCGCCACCAGCCCCACAACCGCCAACACCCTTTTCC10.6493506493506493No Hit
TGAAGCGGGCAGAGTCGATTTTTTNTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGATCAACAAATCCCCCCCACCCCTACCCCTCC10.6493506493506493No Hit
TGAAGCGGGTGTCTACGCAGACTCGCCTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGCCAGTCACAGAGCCACTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGCCCGTGGTATCACCAGAGCCGACTTCTTTTT10.6493506493506493No Hit
TGAAGCGGGGATGCGATGGCCTCAACGGACTTGACGGGTA10.6493506493506493No Hit
TGAAGCGGGAGCGTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGGACAACAAAAAAAAAAAAAAAAAAAAAAAAA10.6493506493506493No Hit
TGAAGCGGGGAAAAAAAAAAAAAAAACAAACCAAAAACAA10.6493506493506493No Hit
TGAAGCGGGGAGTCGACTTTTTTTTTTTTTAAAAAAAAAA10.6493506493506493No Hit
TGAAGCGGTGGTTTATTAATAAATCTGTATTTGCTGTATT10.6493506493506493No Hit
TGAAGCGGGGACTCCCCCCTTAAGGAACCTAACCTTACTG10.6493506493506493No Hit
TGAAGCGGGTACCTGGTTCCTCCCCCCCGTACCCTCTCCT10.6493506493506493No Hit
TGAAGCGGGGGGAGTGGTATCCTCCCGCGTCGCCTTTTTT10.6493506493506493No Hit
TGAAGCGGGCCCCGAGTAGTTTTTTTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGTCTGTCAATGGGAGGGTGAGCCTTGCCCCGC10.6493506493506493No Hit
TGAAGCGGGCGTCAGAAATGGCAGACCCCCCCCCGCCCTC10.6493506493506493No Hit
TGAAGCGGGCCATGGTATTTAAATACCACCACTCCACATG10.6493506493506493No Hit
TGAAGCGGGAAGTGGTCACGCAGAGCCGCCTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGGTACCTGGTTGCTCCTCCCAGTACCATATGC10.6493506493506493No Hit
TGAAGCGGGAAAAAAAAAAAAAAAAAACAAAAAAAAAAAA10.6493506493506493No Hit
TGAAGCGGGACTATGGAGGCGGAGGTCGATCAGCTGGAAC10.6493506493506493No Hit
TGAAGCGGGGATTAAACTGACTTTCTTCTTAAAAAAAAAA10.6493506493506493No Hit
TGAAGCGGGAAATTACCCCCCCCCCCCCCGGCGCCGTAGT10.6493506493506493No Hit
TGAAGCGGGCCGTCCTTGAAACGGGACCCCGTGGCATCTT10.6493506493506493No Hit
TGAAGCGGGGTTTCCGGGTAAAGATGCCAGACCGCGGTTC10.6493506493506493No Hit
TGAAGCGGGCGCAGGATCGCCCCCCGCCGCCTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGATTGCTTTCATAATATTAAAAATTAAAAAAC10.6493506493506493No Hit
TGAAGCGGGCCAATAAAGTTTTAACCNCTGAAAACAAACC10.6493506493506493No Hit
TGAAGCGGGAACCCGTTGAACCGGGACACCCACCCACAAC10.6493506493506493No Hit
TGAAGCGGGATCCCTGTGGTGGTGCCAGGGGGCCGGCCCC10.6493506493506493No Hit
TGAAGCGGGAGTCGACTTTTTTTCTTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGGCTCCTCGTTGGGCGTCTGCCGCCCCGCTCG10.6493506493506493No Hit
TGAAGCGTCATGTTGATCCCATCCACCACCACCTTCCATC10.6493506493506493No Hit
TGAAGCGGGCTGGTTCACCCCCGAGCCCCCTCTTTCTTTC10.6493506493506493No Hit
TGAAGCGGGGTTTATGTTCCCTGTTTAGCCCCAAGAAGGA10.6493506493506493No Hit
TGAAGCGGGCTTTAGTATAAGTTTGAAATTTCGGTTGGGG10.6493506493506493No Hit
TGAAGCGGGGGGGCTGGAGAGATGCCTCAGAGGTTAAGCG10.6493506493506493No Hit
TGAAGCGGGAGGGGCCTTTTTTTAAAAACAAAACTACAAG10.6493506493506493No Hit
TGAAGCGGGATTGGAAGTCCGTTTCCCACTTGCCCAATTT10.6493506493506493No Hit
TGAAGCGGGGCAACAACAAAAAAAAAAAAACACAAAAAAA10.6493506493506493No Hit
TGAAGCGGGGACTTTCTGGTTGTTTTTGGGTTTTGGTTTT10.6493506493506493No Hit
TGAAGCGGGGGGGGCCGGTGAGATGCCCCAGCGGGTAAGA10.6493506493506493No Hit
TGAAGCGGGAATGTAACGCCGATCGCCTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGGCCGAATTTTTTTTTTTCTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGACTCACATAGTCGACCTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGATTATCATCCCAGAGTTGCCTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGTAACGACGGTCTAAACCCGAGTTGAGTTTTCTT10.6493506493506493No Hit
TGAAGCGGGGAGTGCACGTTGGGGTGACCCCGCGCCGGTG10.6493506493506493No Hit
TGAAGCGGGATTGCTTAGTCATCTAGCCCCGTGAGAACAC10.6493506493506493No Hit
TGAAGCGGGGGGTCGGGTTATAGACAGGTTGAGAGCTCTC10.6493506493506493No Hit
TGAAGCGGGCACGCAGAGCGCTTTTTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGGAGATTGGTGTGATTGATGGACATTGGAGGG10.6493506493506493No Hit
TGAAGCGGGAAGTGCAGTGCCTAATCGTCCCGACTCTTCC10.6493506493506493No Hit
TGAAGCGGGAGCTTGGAGTTTTTATGATGATGTCACAGTA10.6493506493506493No Hit
TGAAGCGGGGCGCCCTTGATTGTTTTCTGTTCTCACCGTT10.6493506493506493No Hit
TGAAGCGGGCCCCAGTGAGCCTTGGTCTCAATCCCCCCCA10.6493506493506493No Hit
TGAAGCGGGACACACTTCTTCACTTTGTGTGTATTTGCAT10.6493506493506493No Hit
TGAAGCGGGGAGAACTTAGACTGTAGCCGCTGGAATGCAG10.6493506493506493No Hit
TGAAGCGGGCAGTGGACCCCAGCCCCCTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGGAGTGACGACAGGCCTTGTCCGGCTCGCCGG10.6493506493506493No Hit
TGAAGCGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG10.6493506493506493No Hit
TGAAGCGGGAGTCCTCTGGTTTTCCCCCTCCCGGGCCTGT10.6493506493506493No Hit
TGAAGCGGGGAAAAAAAAAAAAAAAAAAAACAAAAACAAA10.6493506493506493No Hit
TGAAGCGGGGATAAATAGTCATGGTTAGTGCCATATGCTG10.6493506493506493No Hit
TGAAGCGGGATGGTATCCACGCCGCGCCTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGATGGCGGTTGGCGGAGCACCCGTCCCCCCCC10.6493506493506493No Hit
TGAAGCGGGGTTTCCTTTTGCGGCCCTCCGTGGACCCCAG10.6493506493506493No Hit
TGAAGCGGGGTCCAGTGCTTGAAGCGGGAAAACAAACAAC10.6493506493506493No Hit
TGAAGCGGGTACCTGGTTGATCCCCCCAGCACCATATGCT10.6493506493506493No Hit
TGAAGCGGGTAAAAAAAAAAAAAAAAAAAAAAAAACAAAA10.6493506493506493No Hit
TGAAGCGGGAATGTGGCTCTTTTCTTTTAGGTTGTGTGCA10.6493506493506493No Hit
TGAAGCGGGTGGAGACAGCATGAATGTTGACGAGTCAAGT10.6493506493506493No Hit
TGAAGCGGGCAGTCCTTCCAGCGGGCAGTGGTATCACCCA10.6493506493506493No Hit
TGAAGCGGGGTGCTTTTGTGCTCTCCCTCCCCCCCTGGCC10.6493506493506493No Hit
TGAAGCGGGAGTAGAGTCGACTTTTTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGAGTACAGAGTCATTTTTTTTTTTTTTTTTTT10.6493506493506493No Hit
TGAAGCGGGGAGTGGACAGGCACCCGNGCTGGGCCCCACC10.6493506493506493No Hit
TGAAGCGGGTCGAGCGCGTTTTTTTTTTTTTGCCCCATTG10.6493506493506493No Hit
TGAAGCGGGGGGGGCCGGCGAGTTCCTTAATTCTTTGGTG10.6493506493506493No Hit
TGAAGCGGGGGCCATTACTCCCCGAACAGGCCCACCACAG10.6493506493506493No Hit
TGAAGCGGGATGCCTTCANGTGTGCAATTCCCTCTTAGGA10.6493506493506493No Hit
TGAAGCGAGATGGCAGAAACCAAACCGAGCGGGCAGGACT10.6493506493506493No Hit
TGAAGCGGGGAAACATTACACCCACCACCCACACTGCCCA10.6493506493506493No Hit
TGAAGCGGTCCGCCCTTAGGTGTTTACAGTCGGGTCAGGC10.6493506493506493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCGG150.034.02
TGAAGCG150.034.01
AAGCGGG150.034.03