FastQCFastQC Report
Fri 17 Jun 2016
SRR1526051_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526051_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1513
Sequences flagged as poor quality0
Sequence length40
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC181.1896893588896233No Hit
TGAAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.9253139458030404No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG100.6609385327164574No Hit
TGAAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.4626569729015202No Hit
TGAAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.39656311962987445No Hit
TGAAGCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT60.39656311962987445No Hit
TGAAGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG50.3304692663582287No Hit
TGAAGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT50.3304692663582287No Hit
TGAAGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG40.2643754130865829No Hit
TGAAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA40.2643754130865829No Hit
TGAAGCGGGAGATGGGGGTTCACTCTAGGTCATAAATACA30.19828155981493722No Hit
TGAAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.19828155981493722No Hit
TGAAGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.19828155981493722No Hit
TGAAGCGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAGC30.19828155981493722No Hit
TGAAGCGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.19828155981493722No Hit
TGAAGCGGGCAATTATTAATGTAGAGAATGTTGAATGTAA30.19828155981493722No Hit
TGAAGCGGGACCTGTGGGTCGTGTGGGAGAGACATGAGTG30.19828155981493722No Hit
TGAAGCGGGGATCCCCTCGACCACTCTTTTGGCGCTTCGC30.19828155981493722No Hit
TGAAGCGGGGAAAAAAAACAAAAAAAAAAAAAAAAAAAAA20.13218770654329146No Hit
TGAAGCGGGGATGTAGGACCGTATAGGTAGATCCAGACCG20.13218770654329146No Hit
TGAAGCGGGACGGTCAAAAAAAAAAAAAAAAAAAAAAAAA20.13218770654329146No Hit
TGAAGCGGGACTGGCCATGTTAGCTGGGTTGGAAATACAT20.13218770654329146No Hit
TGAAGCGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCA20.13218770654329146No Hit
TGAAGCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC20.13218770654329146No Hit
TGAAGCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.13218770654329146No Hit
TGAAGCGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTC20.13218770654329146No Hit
TGAAGCGGGAACTTTGGAGTAATTGTCTGAGCCCTCAGCC20.13218770654329146No Hit
TGAAGCGGGAGAGTTGGTGTTGAGAGTGTGAGTTGCCTTA20.13218770654329146No Hit
TGAAGCGGGCTCGGAGTTCGAGGCCAACCTGGTCTATAGA20.13218770654329146No Hit
TGAAGCGGGTCAGGTGGCCATTCTTCTGTTCAAATCTGGT20.13218770654329146No Hit
TGAAGCGGGGGGCTGGAGCGATGGCTCAGTGGTTAAAAGC20.13218770654329146No Hit
TGAAGCGGGGTTTACACTTGCCCCTGACGGAGCAGCAAAG20.13218770654329146No Hit
TGAAGCGGGATTTTTGTACATATTGTTAGGGGTCAGCCAT20.13218770654329146No Hit
TGAAGCGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.13218770654329146No Hit
TGAAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA20.13218770654329146No Hit
TGAAGCGGGGGGGGCTGGAGAGATGGCTTAGCGGTTAAGA20.13218770654329146No Hit
TGAAGCGGGGAAAAAAAAAAACAAAAAAAAAAAAAAAAAA20.13218770654329146No Hit
TGAAGCGGGATACTATTGAAATAATTACCGATAGGCAGTC20.13218770654329146No Hit
TGAAGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC20.13218770654329146No Hit
TGAAGCGGGGGCGAGATGGCTTAGTGGTTAAGAGCACCGA20.13218770654329146No Hit
TGAAGCGGGGTTTAATTTAACCTTAATACCAAAACTATCT20.13218770654329146No Hit
TGAAGCGGTGTGTGTATTTACTAACCCTCCCTTCCCTGAG20.13218770654329146No Hit
TGAAGCGGGATGATAGAGAAATGTAAATATGTTAATCCAA20.13218770654329146No Hit
TGAAGCGGGGGCTGTGGGCTATCTTCCTGTTAGCCTTGGA20.13218770654329146No Hit
TGAAGCGGGGTGTGGATGTTGCCTCTTACACCATACTTAC20.13218770654329146No Hit
TGAAGCGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCA20.13218770654329146No Hit
TGAAGCGGGAGCATGTGTTTATTTTCCTTATGTTCTCCAG20.13218770654329146No Hit
TGAAGCGGGGAGGACCATGTGCATGGTTGACATTTTGCAG20.13218770654329146No Hit
TGAAGCGGGGATCTGATTTCTCAACCTCATTTGAACTAAA20.13218770654329146No Hit
TGAAGCGGGACCTCAAGGAATGAGATGTTTTGAGTGGGTT20.13218770654329146No Hit
TGAAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG20.13218770654329146No Hit
TGAAGCGGGAGATAAATTGTCATAAACATCTCTTCCACAG20.13218770654329146No Hit
TGAAGCGGGATTTAAAAAAAAAAAAAAAAAAAAAAAAAAA20.13218770654329146No Hit
TGAAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT20.13218770654329146No Hit
TGAAGCGGGAACTATATACTTGTTTAGGCCATTCTGCCTG20.13218770654329146No Hit
TGAAGCGGGAGTGCAGTGCTTGAAGCGGGTCACAGATCCT20.13218770654329146No Hit
TGAAGCGGGTCACAGATCCTTTCCAATCCTGGTTTCCATT20.13218770654329146No Hit
TGAAGCGGGTCACAAATACTCTTCTGAGATGAGTTTTTGT20.13218770654329146No Hit
TGAAGCGGGCTGAACCTACCTGGTTCCACTTTCAGTCTCC20.13218770654329146No Hit
TGAAGCGGGCAACAAAAAATATCACTTGCTATAGAACTAT20.13218770654329146No Hit
TGAAGCGGGTCCGGTTTAAGCGGATATCAGGGACATCCAT20.13218770654329146No Hit
TGAAGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAG20.13218770654329146No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC20.13218770654329146No Hit
TGAAGCGGGGCGTTTACTCGCCTTCCCTGAAGATGGCACT20.13218770654329146No Hit
TGAAGCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCG20.13218770654329146No Hit
TGAAGCGGGATTTTGAAGACATTAAAATATTGCACTTGTA20.13218770654329146No Hit
TGAAGCGGGATTTTAAAAAATAATAAAAACAAAACATTTT20.13218770654329146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGG307.2196162E-634.05
AGCGGGG750.034.04
GCGGGGT200.00199550533.9999965
GCGGGGA200.00199550533.9999965
CGGGGGG200.00199550533.9999966
AGCGGGC200.00199550533.9999964
CGGGATT200.00199550533.9999966
TGAAGCG1600.031.8749981
GAAGCGG1550.031.8064522
AAGCGGG1550.031.8064523
GCGGGAT303.570481E-428.3333325
AGCGGGA553.769146E-724.7272724
Produced by FastQC (version 0.11.5)