##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526051_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1513 Sequences flagged as poor quality 0 Sequence length 40 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.66424322538004 29.0 4.0 32.0 4.0 33.0 2 12.341705221414408 7.0 4.0 20.0 4.0 28.0 3 12.515532055518836 6.0 4.0 21.0 4.0 27.0 4 12.277594183740913 4.0 4.0 22.0 4.0 30.0 5 11.971579643093193 4.0 4.0 20.0 4.0 28.0 6 12.29676140118969 4.0 4.0 21.0 4.0 29.0 7 13.300066093853271 4.0 4.0 24.0 4.0 31.0 8 13.624586913417053 4.0 4.0 25.0 4.0 31.0 9 13.655651024454725 4.0 4.0 25.0 4.0 31.0 10 11.668208856576339 4.0 4.0 20.0 4.0 28.0 11 12.995373430270984 4.0 4.0 25.0 4.0 32.0 12 13.313284864507601 4.0 4.0 26.0 4.0 33.0 13 12.727032385988103 4.0 4.0 23.0 4.0 32.0 14 12.958360872438863 4.0 4.0 25.0 4.0 33.0 15 12.3694646397885 4.0 4.0 23.0 4.0 33.0 16 10.810971579643093 4.0 4.0 19.0 4.0 30.0 17 10.130204890945143 4.0 4.0 16.0 4.0 28.0 18 9.5783212161269 4.0 4.0 13.0 4.0 26.0 19 9.573694646397884 4.0 4.0 12.0 4.0 28.0 20 10.064111037673497 4.0 4.0 15.0 4.0 29.0 21 8.652346331791144 4.0 4.0 10.0 4.0 24.0 22 8.793787177792465 4.0 4.0 11.0 4.0 25.0 23 8.87111698612029 4.0 4.0 11.0 4.0 26.0 24 8.390614672835426 4.0 4.0 10.0 4.0 23.0 25 9.015862524785195 4.0 4.0 11.0 4.0 26.0 26 9.196959682749505 4.0 4.0 11.0 4.0 27.0 27 9.344348975545275 4.0 4.0 11.0 4.0 27.0 28 9.441506939854593 4.0 4.0 11.0 4.0 28.0 29 8.644415069398546 4.0 4.0 10.0 4.0 25.0 30 9.049570389953734 4.0 4.0 10.0 4.0 27.0 31 10.145406477197621 4.0 4.0 11.0 4.0 31.0 32 9.857237276933246 4.0 4.0 8.0 4.0 31.0 33 9.810971579643093 4.0 4.0 4.0 4.0 31.0 34 9.8473231989425 4.0 4.0 4.0 4.0 31.0 35 9.651685393258427 4.0 4.0 4.0 4.0 31.0 36 9.922009253139459 4.0 4.0 4.0 4.0 32.0 37 9.838070059484467 4.0 4.0 4.0 4.0 32.0 38 9.696629213483146 4.0 4.0 4.0 4.0 32.0 39 9.5743555849306 4.0 4.0 4.0 4.0 32.0 40 9.413747521480502 4.0 4.0 4.0 4.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 11.015151515151512 1 2 18.433982683982684 1 3 -3.8733766233766236 1 4 19.575757575757574 1 5 -6.414502164502165 1 6 -6.481601731601732 1 7 -7.548701298701298 1 8 -8.18939393939394 1 9 -7.770562770562769 1 10 -6.178571428571427 1 11 -7.2813852813852815 1 12 -7.024891774891774 1 13 -6.982683982683982 1 14 -7.107142857142858 1 15 -6.5692640692640705 1 16 -4.624458874458876 1 17 -4.0887445887445875 1 18 -3.7597402597402594 1 19 -4.04978354978355 1 20 -4.262987012987013 1 21 -3.593073593073594 1 22 -3.6071428571428568 1 23 -3.5227272727272725 1 24 -3.1991341991341997 1 25 -3.725108225108226 1 26 -3.266233766233766 1 27 -3.1731601731601717 1 28 -3.075757575757576 1 29 -2.275974025974026 1 30 -3.037878787878787 1 31 -3.742424242424242 1 32 -3.4199134199134207 1 33 -3.6861471861471866 1 34 -3.858225108225109 1 35 -3.6504329004328993 1 36 -3.983766233766234 1 37 -4.123376623376624 1 38 -4.053030303030303 1 39 -3.870129870129869 1 40 -3.8495670995671007 2 1 11.015151515151512 2 2 -7.566017316017316 2 3 -6.873376623376624 2 4 -6.424242424242426 2 5 -6.414502164502165 2 6 -6.481601731601732 2 7 -7.548701298701298 2 8 -8.18939393939394 2 9 -7.770562770562769 2 10 -6.178571428571427 2 11 -7.2813852813852815 2 12 -7.024891774891774 2 13 -6.982683982683982 2 14 -7.107142857142858 2 15 -6.5692640692640705 2 16 -4.624458874458876 2 17 -4.0887445887445875 2 18 -3.7597402597402594 2 19 -4.04978354978355 2 20 -4.262987012987013 2 21 -3.593073593073594 2 22 -3.6071428571428568 2 23 -3.5227272727272725 2 24 -3.1991341991341997 2 25 -3.725108225108226 2 26 -3.266233766233766 2 27 -3.1731601731601717 2 28 -3.075757575757576 2 29 -2.275974025974026 2 30 -3.037878787878787 2 31 -3.742424242424242 2 32 -3.4199134199134207 2 33 -3.6861471861471866 2 34 -3.858225108225109 2 35 -3.6504329004328993 2 36 -3.983766233766234 2 37 -4.123376623376624 2 38 -4.053030303030303 2 39 -3.870129870129869 2 40 -3.8495670995671007 3 1 10.015151515151512 3 2 -3.566017316017316 3 3 11.126623376623376 3 4 -2.4242424242424256 3 5 -2.4145021645021654 3 6 2.5183982683982684 3 7 -2.548701298701298 3 8 5.8106060606060606 3 9 12.22943722943723 3 10 -0.17857142857142705 3 11 21.718614718614717 3 12 16.975108225108226 3 13 17.01731601731602 3 14 2.8928571428571423 3 15 22.430735930735928 3 16 18.375541125541126 3 17 7.9112554112554125 3 18 6.240259740259741 3 19 21.950216450216452 3 20 21.73701298701299 3 21 4.406926406926406 3 22 3.3928571428571432 3 23 16.477272727272727 3 24 9.8008658008658 3 25 12.274891774891774 3 26 13.733766233766234 3 27 21.82683982683983 3 28 10.924242424242424 3 29 3.724025974025974 3 30 7.962121212121213 3 31 23.257575757575758 3 32 21.58008658008658 3 33 23.31385281385281 3 34 6.141774891774891 3 35 23.3495670995671 3 36 16.016233766233768 3 37 20.876623376623378 3 38 24.946969696969695 3 39 27.12987012987013 3 40 27.1504329004329 4 1 -0.48484848484848797 4 2 -0.8160173160173159 4 3 -4.873376623376624 4 4 -1.6742424242424256 4 5 -0.41450216450216537 4 6 0.018398268398268414 4 7 -5.298701298701298 4 8 -0.9393939393939394 4 9 -0.5205627705627691 4 10 -5.178571428571427 4 11 -5.5313852813852815 4 12 -1.274891774891774 4 13 0.26731601731601806 4 14 -1.1071428571428577 4 15 0.6807359307359295 4 16 0.6255411255411243 4 17 0.16125541125541254 4 18 -2.0097402597402594 4 19 0.45021645021645007 4 20 -1.762987012987013 4 21 0.9069264069264058 4 22 2.6428571428571432 4 23 -1.2727272727272725 4 24 -0.4491341991341997 4 25 2.774891774891774 4 26 3.233766233766234 4 27 1.3268398268398283 4 28 -2.325757575757576 4 29 -0.2759740259740262 4 30 2.712121212121213 4 31 3.507575757575758 4 32 -0.16991341991342068 4 33 -0.18614718614718662 4 34 3.391774891774891 4 35 1.0995670995671007 4 36 3.016233766233766 4 37 -0.3733766233766236 4 38 1.4469696969696972 4 39 -1.370129870129869 4 40 3.1504329004328993 5 1 11.015151515151512 5 2 -7.566017316017316 5 3 -6.873376623376624 5 4 -6.424242424242426 5 5 -6.414502164502165 5 6 -6.481601731601732 5 7 -7.548701298701298 5 8 -8.18939393939394 5 9 -7.770562770562769 5 10 -6.178571428571427 5 11 -7.2813852813852815 5 12 -7.024891774891774 5 13 -6.982683982683982 5 14 -7.107142857142858 5 15 -6.5692640692640705 5 16 -4.624458874458876 5 17 -4.0887445887445875 5 18 -3.7597402597402594 5 19 -4.04978354978355 5 20 -4.262987012987013 5 21 -3.593073593073594 5 22 -3.6071428571428568 5 23 -3.5227272727272725 5 24 -3.1991341991341997 5 25 -3.725108225108226 5 26 -3.266233766233766 5 27 -3.1731601731601717 5 28 -3.075757575757576 5 29 -2.275974025974026 5 30 -3.037878787878787 5 31 -3.742424242424242 5 32 -3.4199134199134207 5 33 -3.6861471861471866 5 34 -3.858225108225109 5 35 -3.6504329004328993 5 36 -3.983766233766234 5 37 -4.123376623376624 5 38 -4.053030303030303 5 39 -3.870129870129869 5 40 -3.8495670995671007 6 1 15.015151515151512 6 2 15.683982683982684 6 3 10.876623376623376 6 4 18.075757575757574 6 5 15.085497835497835 6 6 17.01839826839827 6 7 9.951298701298702 6 8 17.060606060606062 6 9 18.22943722943723 6 10 8.821428571428573 6 11 7.9686147186147185 6 12 18.975108225108226 6 13 15.517316017316018 6 14 14.392857142857142 6 15 19.430735930735928 6 16 11.625541125541124 6 17 23.91125541125541 6 18 8.240259740259742 6 19 7.95021645021645 6 20 16.73701298701299 6 21 14.156926406926406 6 22 12.142857142857142 6 23 15.977272727272727 6 24 9.5508658008658 6 25 11.524891774891774 6 26 8.483766233766234 6 27 14.576839826839828 6 28 13.424242424242424 6 29 7.724025974025974 6 30 15.462121212121213 6 31 7.757575757575758 6 32 12.08008658008658 6 33 19.06385281385281 6 34 17.39177489177489 6 35 16.8495670995671 6 36 16.266233766233768 6 37 18.876623376623378 6 38 12.946969696969697 6 39 13.629870129870131 6 40 12.9004329004329 7 1 -8.484848484848488 7 2 -7.566017316017316 7 3 -6.873376623376624 7 4 -6.424242424242426 7 5 -6.414502164502165 7 6 -6.481601731601732 7 7 -7.548701298701298 7 8 -8.18939393939394 7 9 -7.770562770562769 7 10 -6.178571428571427 7 11 -7.2813852813852815 7 12 -7.024891774891774 7 13 -6.982683982683982 7 14 -7.107142857142858 7 15 -6.5692640692640705 7 16 -4.624458874458876 7 17 -4.0887445887445875 7 18 -3.7597402597402594 7 19 -4.04978354978355 7 20 -4.262987012987013 7 21 -3.593073593073594 7 22 -3.6071428571428568 7 23 -3.5227272727272725 7 24 -3.1991341991341997 7 25 -3.725108225108226 7 26 -3.266233766233766 7 27 -3.1731601731601717 7 28 -3.075757575757576 7 29 -2.275974025974026 7 30 -3.037878787878787 7 31 -3.742424242424242 7 32 -3.4199134199134207 7 33 -3.6861471861471866 7 34 -3.858225108225109 7 35 -3.6504329004328993 7 36 -3.983766233766234 7 37 -4.123376623376624 7 38 -4.053030303030303 7 39 -3.870129870129869 7 40 -3.8495670995671007 8 1 -14.984848484848488 8 2 -7.566017316017316 8 3 -6.873376623376624 8 4 -6.424242424242426 8 5 -6.414502164502165 8 6 -6.481601731601732 8 7 -7.548701298701298 8 8 -8.18939393939394 8 9 -7.770562770562769 8 10 -6.178571428571427 8 11 -7.2813852813852815 8 12 -7.024891774891774 8 13 -6.982683982683982 8 14 -7.107142857142858 8 15 -6.5692640692640705 8 16 -4.624458874458876 8 17 -4.0887445887445875 8 18 -3.7597402597402594 8 19 -4.04978354978355 8 20 -4.262987012987013 8 21 -3.593073593073594 8 22 -3.6071428571428568 8 23 -3.5227272727272725 8 24 -3.1991341991341997 8 25 -3.725108225108226 8 26 -3.266233766233766 8 27 -3.1731601731601717 8 28 -3.075757575757576 8 29 -2.275974025974026 8 30 -3.037878787878787 8 31 -3.742424242424242 8 32 -3.4199134199134207 8 33 -3.6861471861471866 8 34 -3.858225108225109 8 35 -3.6504329004328993 8 36 -3.983766233766234 8 37 -4.123376623376624 8 38 -4.053030303030303 8 39 -3.870129870129869 8 40 -3.8495670995671007 9 1 10.765151515151512 9 2 6.183982683982684 9 3 6.376623376623376 9 4 -3.4242424242424256 9 5 9.085497835497835 9 6 1.2683982683982684 9 7 13.201298701298702 9 8 -5.6893939393939394 9 9 -1.5205627705627691 9 10 -1.178571428571427 9 11 -1.2813852813852815 9 12 3.475108225108226 9 13 0.7673160173160181 9 14 4.642857142857142 9 15 -1.8192640692640705 9 16 3.3755411255411243 9 17 -1.0887445887445875 9 18 8.490259740259742 9 19 1.45021645021645 9 20 4.737012987012987 9 21 8.156926406926406 9 22 5.392857142857143 9 23 3.4772727272727275 9 24 6.0508658008658 9 25 6.024891774891774 9 26 0.9837662337662341 9 27 5.576839826839828 9 28 1.9242424242424239 9 29 2.224025974025974 9 30 9.712121212121213 9 31 -1.9924242424242422 9 32 1.5800865800865793 9 33 0.8138528138528134 9 34 2.641774891774891 9 35 3.5995670995671007 9 36 3.266233766233766 9 37 2.8766233766233764 9 38 2.6969696969696972 9 39 0.12987012987013102 9 40 1.1504329004328993 10 1 12.515151515151512 10 2 5.433982683982684 10 3 14.126623376623376 10 4 8.575757575757574 10 5 2.5854978354978346 10 6 20.01839826839827 10 7 17.951298701298704 10 8 17.310606060606062 10 9 19.72943722943723 10 10 14.821428571428573 10 11 16.718614718614717 10 12 8.475108225108226 10 13 10.517316017316018 10 14 3.8928571428571423 10 15 13.93073593073593 10 16 9.875541125541124 10 17 11.911255411255413 10 18 20.24025974025974 10 19 15.45021645021645 10 20 22.73701298701299 10 21 19.406926406926406 10 22 16.892857142857142 10 23 19.477272727272727 10 24 14.3008658008658 10 25 24.774891774891774 10 26 23.733766233766232 10 27 18.82683982683983 10 28 20.924242424242422 10 29 7.224025974025974 10 30 12.462121212121213 10 31 23.757575757575758 10 32 9.58008658008658 10 33 16.31385281385281 10 34 24.14177489177489 10 35 16.3495670995671 10 36 23.516233766233768 10 37 21.376623376623378 10 38 15.946969696969697 10 39 19.12987012987013 10 40 19.6504329004329 11 1 13.015151515151512 11 2 21.433982683982684 11 3 6.126623376623376 11 4 11.575757575757574 11 5 15.585497835497835 11 6 23.51839826839827 11 7 22.451298701298704 11 8 13.81060606060606 11 9 9.22943722943723 11 10 16.821428571428573 11 11 19.718614718614717 11 12 8.975108225108226 11 13 20.01731601731602 11 14 25.892857142857142 11 15 20.430735930735928 11 16 -4.624458874458876 11 17 -4.0887445887445875 11 18 -3.7597402597402594 11 19 -4.04978354978355 11 20 -4.262987012987013 11 21 -3.593073593073594 11 22 -3.6071428571428568 11 23 -3.5227272727272725 11 24 -3.1991341991341997 11 25 -3.725108225108226 11 26 -3.266233766233766 11 27 -3.1731601731601717 11 28 -3.075757575757576 11 29 -2.275974025974026 11 30 -3.037878787878787 11 31 -3.742424242424242 11 32 -3.4199134199134207 11 33 -3.6861471861471866 11 34 -3.858225108225109 11 35 -3.6504329004328993 11 36 -3.983766233766234 11 37 -4.123376623376624 11 38 -4.053030303030303 11 39 -3.870129870129869 11 40 -3.8495670995671007 12 1 19.015151515151512 12 2 26.433982683982684 12 3 27.126623376623378 12 4 22.575757575757574 12 5 24.585497835497833 12 6 21.51839826839827 12 7 7.451298701298702 12 8 20.810606060606062 12 9 21.22943722943723 12 10 13.821428571428573 12 11 24.718614718614717 12 12 19.975108225108226 12 13 20.01731601731602 12 14 19.892857142857142 12 15 25.430735930735928 12 16 7.375541125541124 12 17 8.911255411255413 12 18 14.240259740259742 12 19 17.950216450216452 12 20 9.737012987012987 12 21 2.406926406926406 12 22 2.3928571428571432 12 23 2.4772727272727275 12 24 4.8008658008658 12 25 4.274891774891774 12 26 13.733766233766234 12 27 13.826839826839828 12 28 10.924242424242424 12 29 2.724025974025974 12 30 14.962121212121213 12 31 25.257575757575758 12 32 19.58008658008658 12 33 25.31385281385281 12 34 25.14177489177489 12 35 20.3495670995671 12 36 21.016233766233768 12 37 17.876623376623378 12 38 27.946969696969695 12 39 28.12987012987013 12 40 28.1504329004329 13 1 -6.651515151515154 13 2 -5.566017316017316 13 3 -1.8733766233766236 13 4 -4.090909090909093 13 5 -4.414502164502165 13 6 -1.8149350649350655 13 7 -2.8820346320346317 13 8 -3.5227272727272734 13 9 -2.770562770562769 13 10 -2.51190476190476 13 11 1.3852813852813846 13 12 -6.024891774891774 13 13 1.6839826839826841 13 14 -3.1071428571428577 13 15 2.0974025974025956 13 16 4.708874458874458 13 17 3.2445887445887465 13 18 -0.7597402597402594 13 19 5.283549783549784 13 20 3.070346320346321 13 21 4.74025974025974 13 22 4.726190476190477 13 23 7.143939393939394 13 24 7.467532467532466 13 25 4.94155844155844 13 26 -3.266233766233766 13 27 -3.1731601731601717 13 28 -3.075757575757576 13 29 -2.275974025974026 13 30 -3.037878787878787 13 31 -3.742424242424242 13 32 -3.4199134199134207 13 33 -3.6861471861471866 13 34 -3.858225108225109 13 35 -3.6504329004328993 13 36 -3.983766233766234 13 37 -4.123376623376624 13 38 -4.053030303030303 13 39 -3.870129870129869 13 40 -3.8495670995671007 14 1 -6.651515151515154 14 2 -1.8993506493506498 14 3 -2.8733766233766236 14 4 -4.090909090909093 14 5 -4.081168831168832 14 6 0.8517316017316023 14 7 -2.548701298701298 14 8 -0.5227272727272734 14 9 -2.770562770562769 14 10 0.821428571428573 14 11 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-4.053030303030303 117 39 -3.870129870129869 117 40 -3.8495670995671007 119 1 13.015151515151512 119 2 14.433982683982684 119 3 18.126623376623378 119 4 0.5757575757575744 119 5 15.585497835497835 119 6 2.5183982683982684 119 7 21.451298701298704 119 8 17.810606060606062 119 9 18.22943722943723 119 10 16.821428571428573 119 11 10.718614718614718 119 12 17.975108225108226 119 13 7.017316017316018 119 14 4.892857142857142 119 15 19.430735930735928 119 16 -4.624458874458876 119 17 -4.0887445887445875 119 18 -3.7597402597402594 119 19 -4.04978354978355 119 20 -4.262987012987013 119 21 -3.593073593073594 119 22 -3.6071428571428568 119 23 -3.5227272727272725 119 24 -3.1991341991341997 119 25 -3.725108225108226 119 26 -3.266233766233766 119 27 -3.1731601731601717 119 28 -3.075757575757576 119 29 -2.275974025974026 119 30 -3.037878787878787 119 31 -3.742424242424242 119 32 -3.4199134199134207 119 33 -3.6861471861471866 119 34 -3.858225108225109 119 35 -3.6504329004328993 119 36 -3.983766233766234 119 37 -4.123376623376624 119 38 -4.053030303030303 119 39 -3.870129870129869 119 40 -3.8495670995671007 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 735.0 5 32.0 6 31.0 7 18.0 8 33.0 9 37.0 10 59.0 11 38.0 12 45.0 13 25.0 14 23.0 15 19.0 16 11.0 17 9.0 18 9.0 19 18.0 20 44.0 21 61.0 22 53.0 23 54.0 24 44.0 25 31.0 26 37.0 27 20.0 28 9.0 29 8.0 30 3.0 31 5.0 32 0.0 33 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 98.87566137566137 0.4629629629629629 0.26455026455026454 0.3968253968253968 8 98.6763732627399 0.5294506949040371 0.5956320317670417 0.1985440105890139 9 98.01455989410987 1.0589013898080741 0.5956320317670417 0.3309066843150232 10 49.23992068737608 34.1044282881692 7.270323859881031 9.385327164573695 11 31.52676801057502 22.934567085261072 28.684732319894252 16.853932584269664 12 32.16412971542025 24.950363997352746 22.70019854401059 20.185307743216413 13 24.00793650793651 25.396825396825395 28.240740740740737 22.354497354497354 14 20.383851753805427 31.30377233620119 31.039046988749174 17.27332892124421 15 27.891606080634503 31.98942498347654 26.834104428288168 13.284864507600794 16 30.469266358228687 32.650363516192996 25.512227362855256 11.368142762723068 17 24.52081956378057 29.874421678783875 32.18770654329147 13.417052214144084 18 26.305353602115005 30.006609385327167 27.891606080634503 15.79643093192333 19 22.405816259087903 34.1044282881692 26.23925974884336 17.25049570389954 20 25.5783212161269 31.59286186384666 28.354263053536023 14.474553866490417 21 22.273628552544615 31.46067415730337 27.825512227362854 18.440185062789162 22 19.299405155320557 27.75941837409121 33.37739590218109 19.56378056840714 23 20.48909451421018 33.443489755452745 30.53536021150033 15.53205551883675 24 20.48909451421018 33.57567746199604 26.04097818902842 19.89424983476537 25 21.414408460013217 31.59286186384666 26.371447455386647 20.62128222075347 26 23.560555923229646 32.95830575777631 23.89146260754467 19.589675711449374 27 18.43501326259947 33.02387267904509 25.53050397877984 23.010610079575596 28 20.62128222075347 38.33443489755452 22.20753469927297 18.836748182419036 29 26.591511936339522 33.88594164456234 22.214854111405835 17.307692307692307 30 22.868473231989427 33.04692663582287 28.288169200264374 15.79643093192333 31 25.710508922670193 31.92333113020489 24.38863185723728 17.97752808988764 32 27.27877578176979 33.79906852960745 25.149700598802394 13.77245508982036 33 27.03238598810311 30.138797091870455 26.305353602115005 16.523463317911435 34 19.629874421678785 32.71645736946464 31.13020489094514 16.523463317911435 35 17.448777263714472 35.6906807666887 30.66754791804362 16.192994051553207 36 20.11912640635341 35.671740569159496 27.465254798146923 16.743878226340172 37 22.47191011235955 34.434897554527424 26.63582286847323 16.457369464639786 38 21.943159286186383 35.36021150033047 25.710508922670193 16.986120290812952 39 24.52081956378057 33.37739590218109 23.992068737607404 18.10971579643093 40 20.48909451421018 31.98942498347654 27.29676140118969 20.224719101123593 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.5 12 3.0 13 3.0 14 7.0 15 11.0 16 20.5 17 30.0 18 30.0 19 31.0 20 32.0 21 27.0 22 22.0 23 22.0 24 23.5 25 25.0 26 25.0 27 25.0 28 25.0 29 33.0 30 41.0 31 42.0 32 43.0 33 43.0 34 43.5 35 44.0 36 52.5 37 61.0 38 61.0 39 70.5 40 80.0 41 96.5 42 113.0 43 113.0 44 119.0 45 125.0 46 112.5 47 100.0 48 100.0 49 109.0 50 118.0 51 115.5 52 113.0 53 113.0 54 123.5 55 134.0 56 120.0 57 106.0 58 106.0 59 110.0 60 114.0 61 114.0 62 114.0 63 114.0 64 75.0 65 36.0 66 26.0 67 16.0 68 16.0 69 10.0 70 4.0 71 2.5 72 1.0 73 1.0 74 1.0 75 1.0 76 0.5 77 0.0 78 0.0 79 0.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.06609385327164573 8 0.13218770654329146 9 0.13218770654329146 10 0.0 11 0.0 12 0.13218770654329146 13 0.06609385327164573 14 0.13218770654329146 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.13218770654329146 27 0.3304692663582287 28 0.0 29 0.3304692663582287 30 0.0 31 0.0 32 0.6609385327164574 33 0.0 34 0.0 35 0.0 36 0.13218770654329146 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1513.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.14342366159947 #Duplication Level Percentage of deduplicated Percentage of total 1 95.14140681653373 86.71513549239921 2 3.5532994923857872 6.477197620621282 3 0.580130529369108 1.5862524785194978 4 0.145032632342277 0.5287508261731658 5 0.145032632342277 0.6609385327164574 6 0.145032632342277 0.7931262392597489 7 0.0725163161711385 0.4626569729015202 8 0.0 0.0 9 0.0 0.0 >10 0.21754894851341552 2.775941837409121 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 18 1.1896893588896233 No Hit TGAAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.9253139458030404 No Hit TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 10 0.6609385327164574 No Hit TGAAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.4626569729015202 No Hit TGAAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.39656311962987445 No Hit TGAAGCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 6 0.39656311962987445 No Hit TGAAGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 5 0.3304692663582287 No Hit TGAAGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 5 0.3304692663582287 No Hit TGAAGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG 4 0.2643754130865829 No Hit TGAAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA 4 0.2643754130865829 No Hit TGAAGCGGGAGATGGGGGTTCACTCTAGGTCATAAATACA 3 0.19828155981493722 No Hit TGAAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.19828155981493722 No Hit TGAAGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.19828155981493722 No Hit TGAAGCGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAGC 3 0.19828155981493722 No Hit TGAAGCGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.19828155981493722 No Hit TGAAGCGGGCAATTATTAATGTAGAGAATGTTGAATGTAA 3 0.19828155981493722 No Hit TGAAGCGGGACCTGTGGGTCGTGTGGGAGAGACATGAGTG 3 0.19828155981493722 No Hit TGAAGCGGGGATCCCCTCGACCACTCTTTTGGCGCTTCGC 3 0.19828155981493722 No Hit TGAAGCGGGGAAAAAAAACAAAAAAAAAAAAAAAAAAAAA 2 0.13218770654329146 No Hit TGAAGCGGGGATGTAGGACCGTATAGGTAGATCCAGACCG 2 0.13218770654329146 No Hit TGAAGCGGGACGGTCAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.13218770654329146 No Hit TGAAGCGGGACTGGCCATGTTAGCTGGGTTGGAAATACAT 2 0.13218770654329146 No Hit TGAAGCGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCA 2 0.13218770654329146 No Hit TGAAGCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC 2 0.13218770654329146 No Hit TGAAGCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.13218770654329146 No Hit TGAAGCGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTC 2 0.13218770654329146 No Hit TGAAGCGGGAACTTTGGAGTAATTGTCTGAGCCCTCAGCC 2 0.13218770654329146 No Hit TGAAGCGGGAGAGTTGGTGTTGAGAGTGTGAGTTGCCTTA 2 0.13218770654329146 No Hit TGAAGCGGGCTCGGAGTTCGAGGCCAACCTGGTCTATAGA 2 0.13218770654329146 No Hit TGAAGCGGGTCAGGTGGCCATTCTTCTGTTCAAATCTGGT 2 0.13218770654329146 No Hit TGAAGCGGGGGGCTGGAGCGATGGCTCAGTGGTTAAAAGC 2 0.13218770654329146 No Hit TGAAGCGGGGTTTACACTTGCCCCTGACGGAGCAGCAAAG 2 0.13218770654329146 No Hit TGAAGCGGGATTTTTGTACATATTGTTAGGGGTCAGCCAT 2 0.13218770654329146 No Hit TGAAGCGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.13218770654329146 No Hit TGAAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA 2 0.13218770654329146 No Hit TGAAGCGGGGGGGGCTGGAGAGATGGCTTAGCGGTTAAGA 2 0.13218770654329146 No Hit TGAAGCGGGGAAAAAAAAAAACAAAAAAAAAAAAAAAAAA 2 0.13218770654329146 No Hit TGAAGCGGGATACTATTGAAATAATTACCGATAGGCAGTC 2 0.13218770654329146 No Hit TGAAGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC 2 0.13218770654329146 No Hit TGAAGCGGGGGCGAGATGGCTTAGTGGTTAAGAGCACCGA 2 0.13218770654329146 No Hit TGAAGCGGGGTTTAATTTAACCTTAATACCAAAACTATCT 2 0.13218770654329146 No Hit TGAAGCGGTGTGTGTATTTACTAACCCTCCCTTCCCTGAG 2 0.13218770654329146 No Hit TGAAGCGGGATGATAGAGAAATGTAAATATGTTAATCCAA 2 0.13218770654329146 No Hit TGAAGCGGGGGCTGTGGGCTATCTTCCTGTTAGCCTTGGA 2 0.13218770654329146 No Hit TGAAGCGGGGTGTGGATGTTGCCTCTTACACCATACTTAC 2 0.13218770654329146 No Hit TGAAGCGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCA 2 0.13218770654329146 No Hit TGAAGCGGGAGCATGTGTTTATTTTCCTTATGTTCTCCAG 2 0.13218770654329146 No Hit TGAAGCGGGGAGGACCATGTGCATGGTTGACATTTTGCAG 2 0.13218770654329146 No Hit TGAAGCGGGGATCTGATTTCTCAACCTCATTTGAACTAAA 2 0.13218770654329146 No Hit TGAAGCGGGACCTCAAGGAATGAGATGTTTTGAGTGGGTT 2 0.13218770654329146 No Hit TGAAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG 2 0.13218770654329146 No Hit TGAAGCGGGAGATAAATTGTCATAAACATCTCTTCCACAG 2 0.13218770654329146 No Hit TGAAGCGGGATTTAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.13218770654329146 No Hit TGAAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT 2 0.13218770654329146 No Hit TGAAGCGGGAACTATATACTTGTTTAGGCCATTCTGCCTG 2 0.13218770654329146 No Hit TGAAGCGGGAGTGCAGTGCTTGAAGCGGGTCACAGATCCT 2 0.13218770654329146 No Hit TGAAGCGGGTCACAGATCCTTTCCAATCCTGGTTTCCATT 2 0.13218770654329146 No Hit TGAAGCGGGTCACAAATACTCTTCTGAGATGAGTTTTTGT 2 0.13218770654329146 No Hit TGAAGCGGGCTGAACCTACCTGGTTCCACTTTCAGTCTCC 2 0.13218770654329146 No Hit TGAAGCGGGCAACAAAAAATATCACTTGCTATAGAACTAT 2 0.13218770654329146 No Hit TGAAGCGGGTCCGGTTTAAGCGGATATCAGGGACATCCAT 2 0.13218770654329146 No Hit TGAAGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAG 2 0.13218770654329146 No Hit TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC 2 0.13218770654329146 No Hit TGAAGCGGGGCGTTTACTCGCCTTCCCTGAAGATGGCACT 2 0.13218770654329146 No Hit TGAAGCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCG 2 0.13218770654329146 No Hit TGAAGCGGGATTTTGAAGACATTAAAATATTGCACTTGTA 2 0.13218770654329146 No Hit TGAAGCGGGATTTTAAAAAATAATAAAAACAAAACATTTT 2 0.13218770654329146 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGGGGG 30 7.2196162E-6 34.0 5 AGCGGGG 75 0.0 34.0 4 GCGGGGT 20 0.001995505 33.999996 5 GCGGGGA 20 0.001995505 33.999996 5 CGGGGGG 20 0.001995505 33.999996 6 AGCGGGC 20 0.001995505 33.999996 4 CGGGATT 20 0.001995505 33.999996 6 TGAAGCG 160 0.0 31.874998 1 GAAGCGG 155 0.0 31.806452 2 AAGCGGG 155 0.0 31.806452 3 GCGGGAT 30 3.570481E-4 28.333332 5 AGCGGGA 55 3.769146E-7 24.727272 4 >>END_MODULE