FastQCFastQC Report
Fri 17 Jun 2016
SRR1526050_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526050_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173921
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG51212.9444402918566475No Hit
AAAGTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT15710.9032836747718792No Hit
AAAGTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC13440.7727646460174447No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG13190.7583903036436083No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG6080.3495840065317012No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5540.31853542700421456No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG5380.30933584788495927No Hit
AAAGTTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT4590.26391292598363625No Hit
AAAGTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC4440.2552883205593344No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG4240.2437888466602653No Hit
AAAGTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT3400.195491056284175No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3390.19491608258922155No Hit
AAAGTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC3060.17594195065575752No Hit
AAAGTTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC2870.16501745045164184No Hit
AAAGTTGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT2480.14259347634845704No Hit
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2480.14259347634845704No Hit
AAAGTTGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT2340.13454384461910868No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2230.12821913397462067No Hit
AAAGTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA2170.12476929180489993No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC2170.12476929180489993No Hit
AAAGTTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC2140.12304437072003956No Hit
AAAGTTGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT1950.11211987051592391No Hit
AAAGTTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA1950.11211987051592391No Hit
AAAGTTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC1940.11154489682097045No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1880.10809505465124972No Hit
AAAGTTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT1770.10177034400676169No Hit
AAAGTTGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG1740.10004542292190131No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAATC203.949551E-450.56286640
GTCGGTT150.00806375450.5480839
TATGCAT150.0081667750.3860735
ATATGCA150.00823283550.28351234
TTAAGAC150.00823283550.28351234
TTTATGT150.00823283550.28351234
TAATCCG301.0047825E-650.26889442
TTACTTG150.00827076450.22509446
CACGATT550.050.16681347
CGTTGAT204.135381E-450.09414715
TAGTGGT252.0570242E-550.09414715
CGATTAA550.050.00723349
ACAGGTC252.0818368E-549.9927712
GTACTTG252.0854015E-549.9783259
GATTACG252.0854015E-549.97832550
GCGGGGA150.00843341349.978329
GACTCAC150.00843341349.978329
CTAGTAT150.00851077149.86303329
TCTAGTA150.00852047849.8486628
TCGAGCG150.00852047849.8486632
Produced by FastQC (version 0.11.5)