##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526050_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 173921 Sequences flagged as poor quality 0 Sequence length 56 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.578481034492675 38.0 36.0 40.0 33.0 40.0 2 35.998430321812776 39.0 36.0 40.0 30.0 40.0 3 36.01100499652141 38.0 36.0 40.0 31.0 40.0 4 35.069226832872396 38.0 35.0 40.0 25.0 40.0 5 34.744826674179656 39.0 35.0 40.0 23.0 40.0 6 35.767572633552014 39.0 36.0 40.0 28.0 40.0 7 35.9993962776203 39.0 37.0 40.0 29.0 40.0 8 35.82850834574318 39.0 36.0 40.0 29.0 40.0 9 35.8363452372054 39.0 36.0 40.0 29.0 40.0 10 35.22809206478804 38.0 35.0 40.0 27.0 40.0 11 35.05026995014978 38.0 35.0 40.0 27.0 40.0 12 33.95109848724421 38.0 33.0 39.0 21.0 40.0 13 33.28870579171003 38.0 32.0 39.0 18.0 40.0 14 33.9852116765658 38.0 33.0 39.0 22.0 40.0 15 32.715319024154645 38.0 31.0 39.0 15.0 40.0 16 32.33154133198406 37.0 30.0 39.0 17.0 40.0 17 31.30991657131686 36.0 27.0 39.0 13.0 40.0 18 30.742526779399842 36.0 26.0 39.0 12.0 40.0 19 31.940829457052338 36.0 29.0 39.0 16.0 40.0 20 32.579349244772054 37.0 30.0 39.0 19.0 40.0 21 32.75392275803382 38.0 31.0 39.0 17.0 40.0 22 32.041990328942454 37.0 29.0 39.0 14.0 40.0 23 32.78478734597892 38.0 31.0 39.0 17.0 40.0 24 32.13828692337326 37.0 29.0 39.0 15.0 40.0 25 32.5565457880302 38.0 30.0 39.0 17.0 40.0 26 33.02620155127903 38.0 32.0 39.0 17.0 40.0 27 33.29493850656333 38.0 33.0 39.0 18.0 40.0 28 33.189154846165785 38.0 33.0 39.0 18.0 40.0 29 32.78163649013057 38.0 32.0 39.0 16.0 40.0 30 32.512704043789995 37.0 31.0 39.0 15.0 40.0 31 33.28439923873483 38.0 33.0 39.0 18.0 40.0 32 33.133612387233285 38.0 33.0 39.0 17.0 40.0 33 32.93349279270473 38.0 33.0 39.0 16.0 40.0 34 32.832757401348886 38.0 33.0 39.0 15.0 40.0 35 32.74142282990553 38.0 32.0 39.0 11.0 40.0 36 31.90991312147469 36.0 31.0 39.0 4.0 40.0 37 31.644125781245506 36.0 30.0 39.0 4.0 40.0 38 31.043657752657815 36.0 30.0 38.0 4.0 40.0 39 30.054743245496518 35.0 27.0 38.0 4.0 40.0 40 27.87681763559317 34.0 18.0 38.0 4.0 40.0 41 25.886132209451418 31.0 13.0 38.0 4.0 39.0 42 26.90352516372376 32.0 19.0 38.0 4.0 39.0 43 26.95466907388987 32.0 19.0 38.0 4.0 39.0 44 25.9851541791963 31.0 15.0 38.0 4.0 39.0 45 27.063557592240155 32.0 20.0 38.0 4.0 39.0 46 24.977317287734085 30.0 14.0 36.0 4.0 39.0 47 24.04435347082871 28.0 9.0 36.0 4.0 39.0 48 24.752393328005244 29.0 11.0 36.0 4.0 39.0 49 23.692343075304304 28.0 6.0 36.0 4.0 39.0 50 24.382909481891204 29.0 4.0 36.0 4.0 39.0 51 24.69501670298584 30.0 4.0 36.0 4.0 39.0 52 24.59504602664428 30.0 4.0 36.0 4.0 39.0 53 24.41394656194479 30.0 4.0 36.0 4.0 39.0 54 24.030939334525446 30.0 4.0 36.0 4.0 39.0 55 23.785632557310503 30.0 4.0 36.0 4.0 39.0 56 23.057008641854637 29.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.971474441662295 1 2 3.8656199748835647 1 3 3.7084317988098263 1 4 4.930484880471177 1 5 5.207254308038074 1 6 4.403652093938092 1 7 4.2074729816693335 1 8 4.32866160257808 1 9 4.350772803707727 1 10 4.512673327778906 1 11 1.214873377962867 1 12 -6.285295507841809 1 13 -17.715002433493922 1 14 -5.348397034654564 1 15 1.8419228341407887 1 16 0.15512268418350317 1 17 -4.427924093036491 1 18 -3.2309841116442044 1 19 1.1326212932402484 1 20 0.757406645226343 1 21 6.816763411230291 1 22 7.534098010772588 1 23 6.60986385064081 1 24 7.035748851056514 1 25 6.547779821582292 1 26 6.577206758880319 1 27 6.104007590954996 1 28 6.152225994079338 1 29 6.625152423429039 1 30 6.552700460194121 1 31 5.332692234321225 1 32 5.316311225151722 1 33 5.371189233914009 1 34 5.698828014126047 1 35 5.5913115645096525 1 36 4.8156314609850845 1 37 4.654664420771336 1 38 5.521326982893978 1 39 5.9454695924810075 1 40 -8.02204349386647 1 41 2.32246186856273 1 42 1.449773961200794 1 43 -7.212330287697082 1 44 -13.569323144490635 1 45 0.31298391723107954 1 46 -3.598925157023416 1 47 -8.727219099485175 1 48 -3.1211419450320363 1 49 -12.595458579655423 1 50 -2.1374814599848158 1 51 4.553789629011714 1 52 4.242514563830692 1 53 4.195381641736169 1 54 4.486641077856909 1 55 4.58215867453627 1 56 7.7904183844968315 2 1 3.6296050090486602 2 2 4.561316544768957 2 3 4.5498780092923 2 4 5.553964975595385 2 5 5.8893808984799705 2 6 5.168716786463854 2 7 4.788913011765132 2 8 5.011957092239371 2 9 4.856852423210874 2 10 5.233897581765923 2 11 4.486971619239789 2 12 1.0708156853758872 2 13 2.7478413505149994 2 14 5.336336066690343 2 15 5.896793919336076 2 16 5.821744565439463 2 17 7.684048390807174 2 18 6.111342132158615 2 19 6.971635655921464 2 20 6.4937325397029575 2 21 7.756692739852198 2 22 8.553525921936249 2 23 7.571491710749104 2 24 8.052288103229724 2 25 7.607259325539079 2 26 6.952584635783136 2 27 6.513256673973711 2 28 6.705195938590208 2 29 6.938874225414825 2 30 7.485320809789034 2 31 5.800104130222014 2 32 6.34490223134555 2 33 5.724169926923452 2 34 5.888928646943899 2 35 5.945944839174096 2 36 7.10267011527306 2 37 7.322925448059085 2 38 7.849183060498138 2 39 8.059632082908127 2 40 6.849955323798689 2 41 10.930020750181455 2 42 9.469457149205475 2 43 8.474982453304406 2 44 -1.6475722140737261 2 45 8.79963562557057 2 46 8.4287119199224 2 47 4.782676237010008 2 48 7.88438009610784 2 49 1.1246773888514774 2 50 8.411619025041183 2 51 11.56655347106253 2 52 10.758644029495962 2 53 10.97609883530319 2 54 11.109032887501002 2 55 11.069388114673849 2 56 12.19977495250124 3 1 3.5752500403548453 3 2 4.478147491393472 3 3 4.435948308715773 3 4 5.1711492791103595 3 5 5.903205211913736 3 6 5.017913984405702 3 7 4.6032704601564305 3 8 4.612205061987062 3 9 4.686360823186078 3 10 5.051329855028591 3 11 4.9001512113295576 3 12 5.779116472679835 3 13 6.477219566372664 3 14 5.761628980954804 3 15 1.477997812461119 3 16 7.0579977425518585 3 17 8.337188307737303 3 18 8.629598904832346 3 19 7.280990314653092 3 20 6.593961911719571 3 21 7.813254639300467 3 22 8.561634532685737 3 23 7.516020878190677 3 24 8.107498567553076 3 25 7.613099013097202 3 26 6.715996699511361 3 27 6.463303168301405 3 28 6.2828910597854275 3 29 6.901158026791055 3 30 7.105418757839377 3 31 5.81060564903672 3 32 5.9910494013908036 3 33 5.916743232980117 3 34 6.063313372007446 3 35 6.064635558906289 3 36 7.410561752493319 3 37 7.480733395489505 3 38 7.95873609502792 3 39 8.84244777939315 3 40 10.304459074341118 3 41 12.96503407855539 3 42 11.381799843396777 3 43 11.97981166414069 3 44 12.253277082312113 3 45 11.652614362164709 3 46 13.014069306321257 3 47 13.493940262798858 3 48 13.071907217798994 3 49 13.744465374717198 3 50 12.815623736466392 3 51 11.437906765960552 3 52 11.429079836327524 3 53 11.768845720183236 3 54 11.447871149233066 3 55 11.01680612967602 3 56 10.772287505969715 4 1 3.4840219701794055 4 2 4.613297867333323 4 3 4.482752819993969 4 4 5.445585369335923 4 5 5.798631277076645 4 6 5.229317493177632 4 7 4.70984940752485 4 8 4.868721352713877 4 9 4.947513705391593 4 10 5.000139378838114 4 11 5.0722063491741665 4 12 6.39296359047432 4 13 6.3770942530894565 4 14 6.165576349375854 4 15 6.414777261082669 4 16 7.430679446812508 4 17 9.045772267637052 4 18 9.227217952451394 4 19 7.833245953750843 4 20 7.454989480641881 4 21 7.059620554087431 4 22 7.934002953738371 4 23 7.381183785458848 4 24 7.5947792693074625 4 25 7.150129088285173 4 26 6.520946574198081 4 27 6.33059640138411 4 28 5.716036423193948 4 29 6.192887350099323 4 30 6.445706978390749 4 31 4.857284847031703 4 32 5.342302534222881 4 33 5.4230088971405195 4 34 5.024466254212957 4 35 5.120086686725841 4 36 5.845085562017129 4 37 6.0427008140358645 4 38 6.392633338135692 4 39 6.5894402605961595 4 40 1.5345969189526514 4 41 11.112277186324818 4 42 9.653291071466953 4 43 9.852430711759737 4 44 10.698452520908607 4 45 9.24910559023488 4 46 11.552853767474144 4 47 12.441371340493099 4 48 11.731994937097241 4 49 12.373036803288631 4 50 12.385861831704489 4 51 11.852255136887866 4 52 11.62431793156562 4 53 11.456815644995267 4 54 11.222056612892217 4 55 11.27645525248304 4 56 11.318528107473472 5 1 3.505744816621352 5 2 4.4363181990615175 5 3 4.3579668381855114 5 4 5.568271297639832 5 5 5.763839944813398 5 6 4.93212648194168 5 7 4.509006710895633 5 8 4.624071807502538 5 9 4.676031629415135 5 10 4.751704386863793 5 11 5.28079382910996 5 12 6.172217202031302 5 13 6.5531509679102165 5 14 5.914359120911435 5 15 6.832580899391928 5 16 7.706923427550873 5 17 8.85199217132887 5 18 9.745703773746204 5 19 8.198869281733717 5 20 7.37192367004798 5 21 7.756838317597335 5 22 8.40188684886946 5 23 7.464650880161894 5 24 8.17258825807151 5 25 7.197841768863018 5 26 5.897979747017768 5 27 5.782522565108028 5 28 5.742106514151679 5 29 6.534353374711419 5 30 6.793098631681286 5 31 5.373349587534651 5 32 5.325368344816781 5 33 5.270734953855339 5 34 5.7331339909705825 5 35 5.464241314869234 5 36 6.567116059609429 5 37 6.739396908203869 5 38 6.823786361142648 5 39 7.63334074213709 5 40 9.107376480215358 5 41 12.17410169140775 5 42 10.513871595811523 5 43 10.564830390732446 5 44 11.742179112668904 5 45 10.350978249410911 5 46 11.385210642808541 5 47 11.697725005554624 5 48 11.30175149140436 5 49 12.202892341009335 5 50 11.017870927477624 5 51 10.968480391034472 5 52 11.269529274529773 5 53 11.011818320222666 5 54 10.616331626268355 5 55 10.862972106415622 5 56 9.947607829249826 6 1 3.535537121694553 6 2 4.289271893307344 6 3 4.208835071076216 6 4 5.4686373173878735 6 5 5.832830411275779 6 6 5.164165978026119 6 7 4.668182740858185 6 8 4.687444296436823 6 9 4.633769116647002 6 10 4.740723794422529 6 11 5.259002885970702 6 12 6.212011209521943 6 13 6.744843170838376 6 14 6.118606652406157 6 15 7.078197174502584 6 16 7.745398061531123 6 17 9.146638068502856 6 18 9.451568601802048 6 19 8.08118967669456 6 20 7.359751385403786 6 21 7.627369471836353 6 22 8.436275681011097 6 23 7.663110192385258 6 24 7.9122035117317076 6 25 7.265172378328462 6 26 6.40925826250977 6 27 6.008518479306193 6 28 5.8714476786052 6 29 6.465831073043045 6 30 6.735642043325814 6 31 5.137479652226514 6 32 5.723904265824615 6 33 5.684805433937051 6 34 5.515267120013821 6 35 5.571493613132766 6 36 6.503743570675137 6 37 6.905254926589979 6 38 7.596854117356468 6 39 8.072990044145943 6 40 6.147042806398538 6 41 12.756541255588886 6 42 11.735866829042713 6 43 11.598751058080083 6 44 12.20830100575709 6 45 10.902135893265186 6 46 13.304260693881066 6 47 13.815830214951973 6 48 13.03881311891542 6 49 13.868707798959623 6 50 13.281532827375479 6 51 12.628337387970117 6 52 12.791958624206316 6 53 12.590257203436828 6 54 12.56772760856321 6 55 12.682515858543645 6 56 10.294177458122824 7 1 3.768637354794791 7 2 4.648187977223429 7 3 4.711049523290669 7 4 5.695035918786473 7 5 6.128576332021694 7 6 5.298548262408403 7 7 4.831003253678695 7 8 4.870369704362233 7 9 5.054107111984997 7 10 5.341073444772178 7 11 5.499678876646698 7 12 6.288293260803993 7 13 6.99192941792462 7 14 6.459807118606619 7 15 7.287579458884871 7 16 7.987822303955365 7 17 8.798794245659035 7 18 9.86293223816568 7 19 8.035443755948645 7 20 7.6610334366858375 7 21 8.024455718922596 7 22 8.520541415276831 7 23 7.55151345578852 7 24 8.20439465392285 7 25 7.7317224948785785 7 26 6.631111409362912 7 27 6.560266731054444 7 28 6.3690584012159235 7 29 6.856623613835591 7 30 6.695432253116028 7 31 5.419647484394343 7 32 5.532412424332769 7 33 5.842789116920734 7 34 5.950290430037132 7 35 5.990141631780787 7 36 7.357997649929221 7 37 7.8220964184314745 7 38 8.018182788685142 7 39 9.022407293563194 7 40 8.98981669917243 7 41 12.334804658852292 7 42 11.420598763774649 7 43 12.013144997474022 7 44 12.384990982447068 7 45 11.726028667157959 7 46 13.265216404836774 7 47 13.795492219613976 7 48 13.29545647555878 7 49 14.106003836255663 7 50 12.85536732620998 7 51 12.165167224799955 7 52 12.472669579917266 7 53 12.738683776863404 7 54 12.797331338166941 7 55 12.618762944790731 7 56 11.528967667913033 8 1 3.4981951985258632 8 2 4.512876045061105 8 3 4.495998039341551 8 4 5.368869958762247 8 5 5.843597531294861 8 6 4.840734816012279 8 7 4.496266730359494 8 8 4.63784396238767 8 9 4.54605138818124 8 10 4.989650065002344 8 11 5.1721501062045405 8 12 6.074544017920889 8 13 6.585811913381921 8 14 5.924828317879786 8 15 6.651419555795833 8 16 7.420893170097099 8 17 8.504518049414333 8 18 9.364507041315289 8 19 8.160973737777834 8 20 6.985079469393288 8 21 7.588416954537379 8 22 8.35073523720294 8 23 6.777134291645577 8 24 8.002456434661799 8 25 7.085084093909469 8 26 6.230929701307193 8 27 5.67047266685093 8 28 5.6448609451287055 8 29 6.072189937275926 8 30 6.727231162867692 8 31 4.922554813285924 8 32 4.8263857738179325 8 33 5.098936906139393 8 34 4.569460629915998 8 35 5.0207897238462 8 36 6.473291411285974 8 37 6.066463468704033 8 38 6.491986543984961 8 39 7.075435761158591 8 40 2.7742200910561365 8 41 10.974538153703897 8 42 9.600338315561444 8 43 9.995822162828354 8 44 10.61321630043616 8 45 9.790050472124644 8 46 11.810643418767729 8 47 12.444886526094898 8 48 11.142428007963382 8 49 12.550764587315623 8 50 11.395676229904709 8 51 11.191090907416555 8 52 10.842990597482384 8 53 10.911033867717428 8 54 10.928918255186929 8 55 11.096140291853118 8 56 9.896987050105643 9 1 3.537565277266019 9 2 4.445946911202839 9 3 4.50387205508958 9 4 5.3728069666362614 9 5 5.863282570664943 9 6 4.935223004988657 9 7 4.555321848469731 9 8 4.586662860025463 9 9 4.794082884244233 9 10 4.761303608309426 9 11 5.215457192818711 9 12 5.795016458865771 9 13 6.715733173224439 9 14 5.8578991840215195 9 15 6.8443329416226035 9 16 7.814593957498669 9 17 9.036014112406459 9 18 9.325136962575133 9 19 7.924753265336893 9 20 7.473268445771236 9 21 7.533298844301157 9 22 8.610577756887977 9 23 7.249575236527466 9 24 7.833165096079117 9 25 6.9157927553267875 9 26 6.124630488708764 9 27 5.812204950315493 9 28 6.1448609451287055 9 29 6.631245055386163 9 30 6.6957350998755665 9 31 4.6390902463567905 9 32 5.07048026200691 9 33 4.96507863842286 9 34 5.041901574797887 9 35 5.316065314397385 9 36 6.323685112073374 9 37 6.440479216735529 9 38 6.5904117408353535 9 39 7.04787670604048 9 40 3.9080783587726735 9 41 9.813120830869252 9 42 9.958606032096874 9 43 10.054877280938591 9 44 10.688019450042464 9 45 9.97115283432937 9 46 11.708281214043321 9 47 12.307091250504342 9 48 11.370774464656293 9 49 11.857851201488852 9 50 11.57284158423542 9 51 11.03754760032994 9 52 10.634329180159547 9 53 10.804734655119006 9 54 10.885611168572755 9 55 10.690628480829492 9 56 10.208010672152888 10 1 3.521521970179407 10 2 4.355559772095226 10 3 4.377395677136825 10 4 5.50689489314545 10 5 6.142678896124259 10 6 5.245984159844305 10 7 4.689016074191514 10 8 4.822292781285306 10 9 4.711799419677305 10 10 5.047163188361921 10 11 5.340063492031312 10 12 6.5120112095219405 10 13 6.792570443565648 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4.342087084613926 114 28 4.480166857976556 114 29 5.03636561096889 114 30 4.607042382117459 114 31 4.038806586162142 114 32 4.237644149129714 114 33 4.128598959252315 114 34 3.6859569374427714 114 35 4.06123575504882 114 36 5.943066928476757 114 37 5.959626279874374 114 38 6.317633338135689 114 39 7.269253925192434 114 40 8.67264039721352 114 41 9.987743024834131 114 42 8.0155581522123 114 43 8.217492823560974 114 44 -0.3448704604578552 114 45 8.029229813837365 114 46 2.6371705376604773 114 47 1.4514645081949595 114 48 2.6722123284015886 114 49 -6.0182675445374585 114 50 2.4077562416423746 114 51 4.284087435024514 114 52 4.3330135837395325 114 53 4.83383427853564 114 54 4.868640463823894 114 55 4.442914879812228 114 56 6.956726865237449 115 1 2.8275247950381583 115 2 3.5119863257675377 115 3 3.7245001969108387 115 4 4.076174554162392 115 5 3.963865336802229 115 6 4.038074555324528 115 7 3.7670527973553547 115 8 3.6913605778954732 115 9 3.5137768208072515 115 10 3.9383355047460995 115 11 1.6842725315793317 115 12 -2.109316474093877 115 13 -2.2592657146264443 115 14 -4.901231560228666 115 15 -6.2844761691029625 115 16 2.27030817966158 115 17 3.2451871183230914 115 18 4.200220373758899 115 19 2.9219061635974874 115 20 2.669073419302091 115 21 5.319588269987349 115 22 5.725952106280744 115 23 5.164073212416074 115 24 5.397180399250967 115 25 5.001561695226254 115 26 4.5099901577331885 115 27 4.301661776686775 115 28 4.292287432072072 115 29 4.666010836782132 115 30 4.532894226171219 115 31 4.24289824493324 115 32 4.079066053189791 115 33 4.216713497624781 115 34 3.702028804656866 115 35 3.901745282367486 115 36 6.025251018030044 115 37 6.206038182881709 115 38 6.3587955657386 115 39 6.373580696431514 115 40 9.204310397564434 115 41 9.399646032168242 115 42 7.927443613839838 115 43 6.195630873180235 115 44 -1.3443440892608862 115 45 6.323681861421321 115 46 1.3958117981440346 115 47 -8.25485545530997 115 48 0.3612322252328326 115 49 -1.9990374501415573 115 50 2.3297480302517 115 51 3.663776525104172 115 52 3.911565712033738 115 53 3.5611739985061632 115 54 3.424558630648473 115 55 4.127302710110154 115 56 5.308237550572748 116 1 3.2472295173492185 116 2 4.068217004799628 116 3 4.0413107714764465 116 4 4.507445207610857 116 5 4.713119147696588 116 6 4.3113929648757505 116 7 3.9952267660154135 116 8 3.9152173095871916 116 9 4.137271117790512 116 10 4.548185200940537 116 11 3.6433653788237663 116 12 3.777262781848986 116 13 4.77385975174175 116 14 4.5820857833381154 116 15 -3.12599093298741 116 16 4.0585770209365 116 17 5.79893938354002 116 18 5.467649219297758 116 19 5.1442971666888475 116 20 4.719814278485547 116 21 6.1422351093434955 116 22 6.760889746191204 116 23 6.223501844758037 116 24 6.698081156099921 116 25 5.878094047853903 116 26 5.177482962338242 116 27 4.956202870386807 116 28 4.838071463625212 116 29 5.172806487565634 116 30 4.9530519918678415 116 31 4.655061127355157 116 32 3.8595532080773296 116 33 4.609262266412756 116 34 4.238751968498672 116 35 4.109372401011555 116 36 5.529115211612822 116 37 5.758671164440177 116 38 6.116150858351329 116 39 6.242944303722847 116 40 8.880890719491731 116 41 8.216589854788428 116 42 6.2282910714669555 116 43 1.2810695257759086 116 44 1.874395917135022 116 45 5.67457728834809 116 46 1.0166677836466462 116 47 -5.984841597512293 116 48 0.6919005974746 116 49 -2.432893115848838 116 50 1.1237998370953193 116 51 4.696662144974123 116 52 4.088199333182871 116 53 4.470831817502006 116 54 4.228390844698147 116 55 4.529285441162283 116 56 6.641304387796925 117 1 2.490271970179407 117 2 3.165976438761888 117 3 3.1398956771368276 117 4 3.740228226478777 117 5 3.7718455627909293 117 6 3.3793174931776377 117 7 3.1348494075248468 117 8 3.2097927812853086 117 9 3.1992994196773097 117 10 3.4117465216952567 117 11 0.6338134920313081 117 12 -7.815072123811394 117 13 -7.7220128897676865 117 14 -1.3406736506241472 117 15 -7.9379013103459 117 16 -7.437177696044635 117 17 1.031486553351339 117 18 -2.393317761834318 117 19 0.6796745251794114 117 20 -0.01108194792955075 117 21 4.898013411230288 117 22 5.690252953738373 117 23 4.9106480711731315 117 24 5.319779269307464 117 25 4.914414802570885 117 26 4.895053717055223 117 27 4.376132115669829 117 28 4.701750708908229 117 29 4.851815921527898 117 30 5.59302840696218 117 31 4.01532056131742 117 32 4.258373962794309 117 33 4.653366039997657 117 34 4.8387519684986735 117 35 4.8031224010115565 117 36 6.13972841915999 117 37 5.723057956893012 117 38 6.004240480992838 117 39 7.535868832024729 117 40 9.154239776095508 117 41 7.475670043467673 117 42 5.9782910714669555 117 43 -3.643105002525978 117 44 0.017202520908604413 117 45 6.09285559023488 117 46 0.04838948175985536 117 47 -1.343450088078331 117 48 0.606994937097241 117 49 -1.299284625282798 117 50 0.7260404031330552 117 51 5.453147994030726 117 52 5.464496502994191 117 53 5.0800299307095536 117 54 6.099735184320789 117 55 5.401455252483036 117 56 7.782813821759188 118 1 2.3332973324982476 118 2 3.05030614890682 118 3 3.0830116191658092 118 4 3.9592499656092173 118 5 4.2651426642402015 118 6 3.425694304771838 118 7 2.9870233205683263 118 8 3.2067130711403777 118 9 3.2212197095323774 118 10 3.4861124637242398 118 11 0.26361421666899076 118 12 -8.532554007869365 118 13 -7.830255643390874 118 14 -11.030438143377772 118 15 -9.759912179911119 118 16 -11.896235667059127 118 17 -0.9681511278080812 118 18 -8.044314138645912 118 19 -1.271412431342327 118 20 -1.238074701552737 118 21 3.918212686592611 118 22 5.419147881274604 118 23 4.065448795810813 118 24 4.883547385249496 118 25 4.475646686628853 118 26 4.441702267779863 118 27 4.489085014220553 118 28 4.584721723400982 118 29 5.346924617180068 118 30 5.356524783773775 118 31 4.528454619288436 118 32 4.565801499026193 118 33 4.26731531535998 118 34 4.970636026469688 118 35 4.649227473475321 118 36 5.8395472597397 118 37 5.788818826458229 118 38 6.054693379543561 118 39 7.1905789769522706 118 40 8.942826732617249 118 41 8.209275115931444 118 42 7.202928752626377 118 43 2.188507316314599 118 44 -0.4649532761928441 118 45 6.77664182211894 118 46 0.07402353973087017 118 47 0.3479448394578988 118 48 0.4910529081117332 118 49 -0.9903172339784518 118 50 1.612725185741752 118 51 6.60396321142203 118 52 5.940674039226074 118 53 5.67441398868057 118 54 6.784789532146874 118 55 6.996745107555501 118 56 8.869770343498317 119 1 2.7494065855640173 119 2 3.5174187464541973 119 3 3.6482290104701605 119 4 4.688625662376218 119 5 4.513191716637081 119 6 3.980599544459686 119 7 3.539977612653054 119 8 3.617805601798125 119 9 3.5938507017285914 119 10 3.948765752464489 119 11 1.0124993894672087 119 12 -6.771963149452418 119 13 -2.3670449410497376 119 14 -5.617115958316454 119 15 -7.929087207781798 119 16 -8.839100772967711 119 17 0.18597373283851937 119 18 -5.562708787475344 119 19 -0.0991716286667419 119 20 -0.4607614351090348 119 21 5.188558283025159 119 22 5.817977312712728 119 23 5.045103199378264 119 24 4.689010038538232 119 25 4.830440443596526 119 26 5.514444742696252 119 27 5.138471859259571 119 28 5.199827631985151 119 29 5.519444126656101 119 30 5.79799635568013 119 31 4.713237227984084 119 32 4.856771398691748 119 33 5.0633019374335575 119 34 4.937469917216621 119 35 5.013218554857708 119 36 6.631074573006142 119 37 7.092609238944295 119 38 6.7040802245825795 119 39 7.809586780742681 119 40 10.238534647890376 119 41 6.90659953064716 119 42 6.023162866338751 119 43 -2.3753165409875194 119 44 -0.4624449149888292 119 45 6.195259436388728 119 46 0.015216404836777997 119 47 -2.0865590624373063 119 48 -5.48274865264635 119 49 -2.8568166765648506 119 50 1.2643416851843376 119 51 6.279269788902521 119 52 6.385490092737783 119 53 5.904068392248014 119 54 5.97681851765412 119 55 6.371327047354832 119 56 8.103326642272009 120 1 2.2790836985744676 120 2 3.129479525181644 120 3 3.1235376524454637 120 4 4.0335924240096475 120 5 4.303790007235371 120 6 3.7842557647825714 120 7 3.3163308890063306 120 8 3.300379201038396 120 9 3.382787073998294 120 10 3.4587372624359958 120 11 0.8218536154881022 120 12 -6.286445580601516 120 13 -4.963911037915835 120 14 -4.923621181488343 120 15 0.19365733162941012 120 16 -8.036869054069324 120 17 0.8243877879192425 120 18 -5.907438132204689 120 19 0.4435634140683007 120 20 0.2640723730581058 120 21 4.193306621106835 120 22 5.455916533985281 120 23 4.191589429197823 120 24 4.604964454492649 120 25 4.359167888990637 120 26 5.55361853187004 120 27 5.147968535422915 120 28 4.983540832365023 120 29 5.589007279552586 120 30 5.003599394616504 120 31 4.228669326749522 120 32 3.6060591479794937 120 33 3.9806808548124764 120 34 3.973319869733242 120 35 3.6371501787893337 120 36 5.285870394468628 120 37 5.421669068004125 120 38 5.3925892464249365 120 39 6.4133379678272 120 40 6.443900269922668 120 41 -4.315147857766892 120 42 -2.4723262124836616 120 43 -6.204138953143264 120 44 -13.227164763042012 120 45 -1.7681783603824037 120 46 -7.133169160215454 120 47 -5.195533421411664 120 48 -3.7201655567299206 120 49 -13.549361785776627 120 50 -2.9065984857558327 120 51 0.987484413783811 120 52 0.8142650215127105 120 53 1.4301842516972094 120 54 1.010769134938073 120 55 0.872751548779334 120 56 4.0482459205246215 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 6432.0 5 1003.0 6 69.0 7 659.0 8 1026.0 9 922.0 10 682.0 11 510.0 12 446.0 13 405.0 14 472.0 15 520.0 16 703.0 17 902.0 18 1185.0 19 1426.0 20 1961.0 21 2446.0 22 2836.0 23 3281.0 24 4010.0 25 4455.0 26 5018.0 27 5313.0 28 5717.0 29 5973.0 30 6647.0 31 7639.0 32 8885.0 33 10025.0 34 11062.0 35 11867.0 36 13013.0 37 14460.0 38 15658.0 39 16287.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.70618844187878 0.19089126672454734 0.07934636990357692 0.023573921493091694 8 99.57796930790417 0.32543511134365605 0.05749736949534559 0.039098211256835 9 98.03816719277366 0.5726803856967898 0.8078473312289055 0.5813050903006572 10 45.9035999103041 25.762271376084545 19.87971550301574 8.454413210595616 11 31.801795067875645 29.025822068640363 18.19159273463239 20.980790128851606 12 30.31261319794619 21.168806527101385 18.101896838219652 30.41668343673277 13 22.556793026719028 14.559483903611408 22.030692095836617 40.85303097383295 14 22.83738018985631 26.30734643890042 29.361606706493177 21.493666664750087 15 37.0969605917023 17.75800300473824 21.573442736623136 23.571593666936323 16 34.408441304397854 25.91132948242929 21.114192345752883 18.56603686741997 17 26.976805709717677 18.92292916338373 35.24002323454816 18.860241892350427 18 24.025850817325107 18.954582827835626 29.099418701594402 27.920147653244864 19 30.570606844981306 22.392867414437735 20.14667817083693 26.88984756974403 20 23.548914419911128 29.086178712039235 27.620703151981214 19.74420371606843 21 22.210914276186042 21.641683772330797 29.739706415055284 26.407695536427877 22 20.77034975649864 20.647880359473554 30.39828427849426 28.183485605533548 23 21.975494621121083 21.847850460841418 27.945446495822818 28.23120842221468 24 22.17264044071559 22.0001265102157 28.472274136136495 27.35495891293222 25 30.83986935621133 20.996733905283254 26.288612369758724 21.874784368746695 26 30.46672304391097 20.952775428725932 21.092567983478016 27.487933543885084 27 21.48146443346126 26.499272156086555 20.48607315262858 31.533190257823602 28 25.181841704280828 32.634907857286606 20.303021591007102 21.880228847425467 29 36.58787966028502 24.58730387217504 19.089103210018713 19.73571325752123 30 28.936211825748813 20.803492846911833 31.18711926415518 19.073176063184174 31 29.201315804607614 31.06863117214727 21.196070989038795 18.53398203420632 32 36.95367132867133 23.980953257269046 20.334353146853147 18.73102226720648 33 29.29717407447917 24.37871661879076 20.705992067375497 25.618117239354564 34 22.61852653888684 28.43024327312938 30.327359012163658 18.623871175820124 35 21.51354962051849 24.901818516854966 36.14287853136624 17.441753331260294 36 25.937239336152096 30.937958408835964 24.27014122587511 18.854661029136825 37 26.881974273639113 23.565415663829935 31.029975701601852 18.522634360929096 38 33.53922743615747 24.786807412187702 22.466465382133308 19.207499769521526 39 27.02839639805624 23.433937495681814 19.575781303977337 29.96188480228461 40 21.908991812974 18.572720736600413 27.954171208572028 31.56411624185356 41 19.187421895434333 16.78804963817606 24.912086953994596 39.112441512395016 42 26.041006007910607 26.59221162605657 22.040729251951706 25.326053114081116 43 17.93754036350217 22.25066888089307 31.654788264600054 28.157002491004707 44 20.547415112538335 23.575005342466547 24.661403133862 31.216176411133123 45 24.32113205155536 22.02395699494702 31.202862394201464 22.452048559296156 46 19.885314749861546 27.31504984308658 22.85628576702972 29.943349640022156 47 17.3444741069468 28.230997700193 22.857076818176562 31.567451374683632 48 23.79475613194249 28.021380528529427 24.190031242268546 23.993832097259542 49 20.535096619471474 28.991029839044412 21.92673984227275 28.547133699211365 50 26.457982902831862 25.308762874720614 23.76552455125694 24.46772967119058 51 20.01499466535944 30.628334150350355 26.204331151417282 23.15234003287292 52 20.014513704507888 28.065012181144855 30.575537496616352 21.34493661773091 53 20.180667327691562 26.153526875273826 31.793183757234765 21.872622039799847 54 20.50272631906579 28.3191349556757 27.735253092325962 23.44288563293255 55 19.62028965453623 27.876366403763665 27.501037775010378 25.00230616668973 56 25.68729611656081 22.026327619794127 27.741403755489724 24.54497250815534 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 6.0 5 12.0 6 72.5 7 133.0 8 214.0 9 295.0 10 347.0 11 399.0 12 387.0 13 371.5 14 368.0 15 357.5 16 347.0 17 354.0 18 361.0 19 396.5 20 432.0 21 478.0 22 645.5 23 767.0 24 824.0 25 881.0 26 959.5 27 1038.0 28 1179.0 29 1480.0 30 1640.0 31 1973.5 32 2307.0 33 2411.5 34 2516.0 35 2729.0 36 2942.0 37 3272.0 38 4113.0 39 4624.0 40 6019.5 41 7415.0 42 12222.0 43 17029.0 44 16304.0 45 15579.0 46 13528.5 47 10870.5 48 10263.0 49 10395.0 50 10527.0 51 10618.5 52 10710.0 53 10388.0 54 10239.0 55 10412.0 56 10757.5 57 11103.0 58 10305.0 59 9507.0 60 8228.5 61 6950.0 62 6243.0 63 4573.0 64 3610.0 65 3250.5 66 2891.0 67 2479.5 68 2068.0 69 1850.0 70 1632.0 71 1227.0 72 620.5 73 419.0 74 374.5 75 330.0 76 410.0 77 490.0 78 323.5 79 98.0 80 39.0 81 21.0 82 3.0 83 2.0 84 1.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0011499473899069118 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.49505235135492553 16 0.06267213274992668 17 0.024148895188045146 18 0.0 19 0.040823132341695365 20 0.10867002834620315 21 0.0011499473899069118 22 0.0 23 0.0 24 0.012649421288976028 25 0.0074746580343949265 26 0.05289757993571794 27 0.0689968433944147 28 0.003449842169720735 29 0.14144352895855014 30 0.04599789559627647 31 0.020124079323370955 32 0.018974131933464044 33 0.11959452855031882 34 0.16616739784154874 35 0.1512180817727589 36 0.04944773776599721 37 0.1420185026535036 38 0.21216529343782523 39 0.13626876570396904 40 0.8354367787673714 41 1.0786506517326833 42 0.2777122946625192 43 0.28576192639186754 44 0.44847948206369564 45 0.20699053018324415 46 0.331759821988144 47 0.49620229874483246 48 0.0678468960045078 49 0.2627629785937293 50 0.18686645085987316 51 0.3024361635455178 52 0.1678923189264091 53 0.2604630838139155 54 0.24551376774512565 55 0.2719625577129846 56 0.23861408340568419 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 173921.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.231969576970485 #Duplication Level Percentage of deduplicated Percentage of total 1 87.16239175600433 55.11449703771648 2 7.525516390306125 9.51706446885659 3 2.217822709506086 4.207118942838092 4 0.8663211526021551 2.191167710609019 5 0.5095366183641872 1.6109501975378353 6 0.30971409406380246 1.1750299304040799 7 0.254764439035407 1.1276480080866564 8 0.15285863855998413 0.7732442226401635 9 0.13087896272225372 0.7448161130207102 >10 0.7592977973800159 8.85377252040894 >50 0.06294179109645785 2.7290972021014315 >100 0.04096211801515511 4.971758097346821 >500 0.0029972281474503737 1.0739502878639893 >1k 0.0029972281474503737 2.6747679522447827 >5k 9.990760491501247E-4 3.2351173083244054 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 5121 2.9444402918566475 No Hit AAAGTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 1571 0.9032836747718792 No Hit AAAGTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 1344 0.7727646460174447 No Hit AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 1319 0.7583903036436083 No Hit AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 608 0.3495840065317012 No Hit AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 554 0.31853542700421456 No Hit AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 538 0.30933584788495927 No Hit AAAGTTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 459 0.26391292598363625 No Hit AAAGTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 444 0.2552883205593344 No Hit AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 424 0.2437888466602653 No Hit AAAGTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 340 0.195491056284175 No Hit AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 339 0.19491608258922155 No Hit AAAGTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 306 0.17594195065575752 No Hit AAAGTTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 287 0.16501745045164184 No Hit AAAGTTGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 248 0.14259347634845704 No Hit AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 248 0.14259347634845704 No Hit AAAGTTGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT 234 0.13454384461910868 No Hit AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 223 0.12821913397462067 No Hit AAAGTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 217 0.12476929180489993 No Hit AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 217 0.12476929180489993 No Hit AAAGTTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 214 0.12304437072003956 No Hit AAAGTTGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT 195 0.11211987051592391 No Hit AAAGTTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 195 0.11211987051592391 No Hit AAAGTTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC 194 0.11154489682097045 No Hit AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 188 0.10809505465124972 No Hit AAAGTTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 177 0.10177034400676169 No Hit AAAGTTGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG 174 0.10004542292190131 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.010349526509162205 0.0 0.0 0.0 0.0 11 0.0195491056284175 0.0 0.0 0.0 0.0 12 0.021849000408231324 0.0 0.0 0.0 0.0 13 0.021849000408231324 0.0 0.0 0.0 0.0 14 0.034498421697207356 0.0 0.0 0.0 0.0 15 0.035648369087114266 0.0 0.0 0.0 0.0 16 0.03852323756188154 0.0 0.0 0.0 0.0 17 0.04197307973160228 0.0 0.0 0.0 0.0 18 0.04197307973160228 0.0 0.0 0.0 0.0 19 0.048872764071043746 0.0 0.0 0.0 0.0 20 0.05577244841048522 0.0 0.0 0.0 0.0 21 0.058072343190299046 0.0 0.0 0.0 0.0 22 0.06439705383478705 0.0 0.0 0.0 0.0 23 0.06957181708936816 0.0 0.0 0.0 0.0 24 0.07589652773385618 0.0 0.0 0.0 0.0 25 0.07819642251367 0.0 0.0 0.0 0.0 26 0.08509610685311148 0.0 0.0 0.0 0.0 27 0.09429568597236676 0.0 0.0 0.0 0.0 28 0.09947044922694787 0.0 0.0 0.0 0.0 29 0.1029202913966686 0.0 0.0 0.0 0.0 30 0.10464521248152897 0.0 0.0 0.0 0.0 31 0.11671966007555154 0.0 0.0 0.0 0.0 32 0.12016950224527227 0.0 0.0 0.0 0.0 33 0.12361934441499302 0.0 0.0 0.0 0.0 34 0.1253442654998534 0.0 0.0 0.0 0.0 35 0.1276441602796672 0.0 0.0 0.0 0.0 36 0.13109400244938793 0.0 0.0 0.0 0.0 37 0.13339389722920175 0.0 0.0 0.0 0.0 38 0.1356937920090156 0.0 0.0 0.0 5.749736949534559E-4 39 0.13914363417873632 0.0 0.0 0.0 5.749736949534559E-4 40 0.14086855526359668 0.0 0.0 0.0 5.749736949534559E-4 41 0.14374342373836396 0.0 0.0 0.0 5.749736949534559E-4 42 0.1471932659080847 0.0 0.0 0.0 5.749736949534559E-4 43 0.15639284502734 0.0 0.0 0.0 5.749736949534559E-4 44 0.1667423715365022 0.0 0.0 0.0 5.749736949534559E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTAATC 20 3.949551E-4 50.562866 40 GTCGGTT 15 0.008063754 50.54808 39 TATGCAT 15 0.00816677 50.38607 35 ATATGCA 15 0.008232835 50.283512 34 TTAAGAC 15 0.008232835 50.283512 34 TTTATGT 15 0.008232835 50.283512 34 TAATCCG 30 1.0047825E-6 50.268894 42 TTACTTG 15 0.008270764 50.225094 46 CACGATT 55 0.0 50.166813 47 CGTTGAT 20 4.135381E-4 50.094147 15 TAGTGGT 25 2.0570242E-5 50.094147 15 CGATTAA 55 0.0 50.007233 49 ACAGGTC 25 2.0818368E-5 49.99277 12 GTACTTG 25 2.0854015E-5 49.978325 9 GATTACG 25 2.0854015E-5 49.978325 50 GCGGGGA 15 0.008433413 49.97832 9 GACTCAC 15 0.008433413 49.97832 9 CTAGTAT 15 0.008510771 49.863033 29 TCTAGTA 15 0.008520478 49.84866 28 TCGAGCG 15 0.008520478 49.84866 32 >>END_MODULE