FastQCFastQC Report
Fri 17 Jun 2016
SRR1526049_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526049_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5979
Sequences flagged as poor quality0
Sequence length40
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGATGAGATGGACTTAAAGGAATTGACGGGTAC230.38467971232647596No Hit
AAAGTTGGGATGAGATGGACTTAAATGAATTGACGGGTAC210.35122930255895635No Hit
AAAGTTGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC180.3010536879076769No Hit
AAAGTTGGGATGAGATGGCCTTAAATGAATTGACGGGTAC160.2676032781401572No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGATGAGATGGA160.2676032781401572No Hit
AAAGTTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGC120.2007024586051179No Hit
AAAGTTGGGCTCGTCTTAATCAGCAACATCTTTTGATACA100.16725204883759828No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC100.16725204883759828No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGACACGCAGG100.16725204883759828No Hit
AAAGTTGGGATGAGATGGACTCAAATGAATTGACGGGTAC90.15052684395383845No Hit
AAAGTTGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC80.1338016390700786No Hit
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG80.1338016390700786No Hit
AAAGTTGGGGCCGAATAGCCTCTCCACCCCAGCGGCCGGA70.11707643418631879No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT70.11707643418631879No Hit
AAAGTTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGA60.10035122930255895No Hit
AAAGTTGGGATGAGATGGACTCAAAGGAATTGACGGGTAC60.10035122930255895No Hit
AAAGTTGGGCTCGTCTTACTCAGCAACATCTTTTGATACA60.10035122930255895No Hit
AAAGTTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCCGA60.10035122930255895No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAA60.10035122930255895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGTG406.071605E-833.966396
TGGGGGG406.071605E-833.966396
TGGGGCA200.002585451133.966396
GGGACTG200.002585451133.966397
GGAAGCA200.002585451133.966398
AGTTGGA200.002585451133.966393
GGGAAGC301.2603234E-533.966397
TGGGATT200.002585451133.966396
TGGGAGT406.071605E-833.966396
TGGGAGC200.002585451133.966396
TGGGACT200.002585451133.966396
TGGGAAG454.1873136E-933.966396
TTGGGGT552.0008883E-1133.9663855
TTGGGGC552.0008883E-1133.9663855
GGGGTGC251.8072085E-433.9663857
TGGGAGA251.8072085E-433.9663856
TTGGGAG900.032.0793655
TTGGGGG558.0035534E-1030.8785325
TTGGGAA501.1719749E-830.5697485
TGGGGAG501.1719749E-830.5697486
Produced by FastQC (version 0.11.5)