FastQCFastQC Report
Fri 17 Jun 2016
SRR1526047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8949
Sequences flagged as poor quality0
Sequence length40
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT2572.8718292546653257No Hit
AAAGTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT1181.318583081908593No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC1001.117443289753045No Hit
AAAGTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG850.9498267962900884No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG360.4022795843110962No Hit
AAAGTTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC210.23466309084813944No Hit
AAAGTTGGGACTAATCCTAGCCCTAGCCCTACACAAATAT200.223488657950609No Hit
AAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.2011397921555481No Hit
AAAGTTGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG170.18996535925801766No Hit
AAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.16761649346295676No Hit
AAAGTTGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT150.16761649346295676No Hit
AAAGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA140.1564420605654263No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA130.14526762766789586No Hit
AAAGTTGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT120.1340931947703654No Hit
AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.1340931947703654No Hit
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG100.1117443289753045No Hit
AAAGTTGGGTACATGGTTGATCCTGCCAGTAGCATATGCT100.1117443289753045No Hit
AAAGTTGGGAGTGCAGTGCTAAAGTTGGGTCACAGATCCT100.1117443289753045No Hit
AAAGTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGC100.1117443289753045No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAG100.1117443289753045No Hit
AAAGTTGGGGTTGGGCGGATTGAACAAAAACTCATCTCAG90.10056989607777406No Hit
AAAGTTGGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCC90.10056989607777406No Hit
AAAGTTGGGGGGGAGGTAGTGACGAAAAATAACAATACAG90.10056989607777406No Hit
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG90.10056989607777406No Hit
AAAGTTGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT90.10056989607777406No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTACCATATGCT90.10056989607777406No Hit
AAAGTTGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT90.10056989607777406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTAG358.524421E-734.4114326
GATGAGT301.267777E-534.2159128
CTTCTGA503.092282E-1034.2159121
CTGAGAT358.9211244E-734.21590824
CCTGCCA358.9211244E-734.21590822
TTCTGAG358.9211244E-734.21590822
GCCAGTA358.9211244E-734.21590825
TCTGAGA358.9211244E-734.21590823
CTGCCAG358.9211244E-734.21590823
TCCTGCC358.9211244E-734.21590821
ATGAGTT358.9211244E-734.21590829
ATCCTGC359.3337985E-734.022620
GTACCTG301.3188317E-534.02269
GGGTACC359.3337985E-734.02267
GAGTTTT359.3337985E-734.022631
GGGAGGA200.00263421634.02267
TTGGGTG251.8634034E-434.02265
TGGGGTG251.8634034E-434.02266
TTGGGGT552.3646862E-1134.02265
TTGGGGC359.3337985E-734.02265
Produced by FastQC (version 0.11.5)