##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526047_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8949 Sequences flagged as poor quality 0 Sequence length 40 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.723097552799196 30.0 19.0 33.0 4.0 36.0 2 20.005363727790815 23.0 4.0 31.0 4.0 35.0 3 18.464632919879318 20.0 4.0 30.0 4.0 35.0 4 16.104927924907813 12.0 4.0 28.0 4.0 35.0 5 16.087495809587665 14.0 4.0 26.0 4.0 34.0 6 16.890937534920102 16.0 4.0 29.0 4.0 35.0 7 18.565314560286065 21.0 4.0 31.0 4.0 35.0 8 18.785674377025366 23.0 4.0 31.0 4.0 35.0 9 18.217901441501844 20.0 4.0 31.0 4.0 35.0 10 16.180914068611017 12.0 4.0 29.0 4.0 34.0 11 15.704883227176222 9.0 4.0 29.0 4.0 34.0 12 14.992066152642753 4.0 4.0 28.0 4.0 33.0 13 13.669572019220025 4.0 4.0 25.0 4.0 32.0 14 14.534473125488882 4.0 4.0 28.0 4.0 33.0 15 14.196670018996535 4.0 4.0 28.0 4.0 33.0 16 11.951726449882669 4.0 4.0 22.0 4.0 31.0 17 10.909151860543078 4.0 4.0 19.0 4.0 29.0 18 10.795619622304168 4.0 4.0 18.0 4.0 29.0 19 10.471114090959883 4.0 4.0 17.0 4.0 29.0 20 11.00167616493463 4.0 4.0 20.0 4.0 30.0 21 9.627444407196334 4.0 4.0 13.0 4.0 27.0 22 9.339814504413901 4.0 4.0 13.0 4.0 26.0 23 9.389205497820985 4.0 4.0 13.0 4.0 26.0 24 9.000558721644877 4.0 4.0 12.0 4.0 25.0 25 9.40574365850933 4.0 4.0 13.0 4.0 27.0 26 9.411554363616046 4.0 4.0 13.0 4.0 27.0 27 9.595262040451447 4.0 4.0 13.0 4.0 28.0 28 9.900659291540954 4.0 4.0 15.0 4.0 29.0 29 8.826349312772377 4.0 4.0 10.0 4.0 25.0 30 9.206391775617387 4.0 4.0 12.0 4.0 27.0 31 10.243043915521287 4.0 4.0 15.0 4.0 31.0 32 10.046597385182702 4.0 4.0 14.0 4.0 31.0 33 9.895407308079115 4.0 4.0 12.0 4.0 30.0 34 10.04950273773606 4.0 4.0 11.0 4.0 31.0 35 10.012291876187284 4.0 4.0 4.0 4.0 31.0 36 9.882333221589004 4.0 4.0 4.0 4.0 32.0 37 9.604648564085373 4.0 4.0 4.0 4.0 32.0 38 9.486758297016426 4.0 4.0 4.0 4.0 31.0 39 9.266621968935077 4.0 4.0 4.0 4.0 31.0 40 9.340037993071851 4.0 4.0 4.0 4.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 5.074965187520714 1 2 4.763467944461944 1 3 0.963877174740567 1 4 7.630534088088913 1 5 9.409880204796988 1 6 -2.1732759480272037 1 7 -5.981097598319234 1 8 -6.971060058173615 1 9 -6.190869335833522 1 10 -5.873198563659186 1 11 -7.768569506946079 1 12 -5.128979968444714 1 13 -4.217257733797963 1 14 -6.785512610517904 1 15 -6.909733348197625 1 16 -2.8022007229231196 1 17 -2.2753818453043975 1 18 -2.3318434589122123 1 19 -4.3155700659231755 1 20 -4.641827881930121 1 21 -3.560203312537169 1 22 -3.2985164487004823 1 23 -3.2853703221658144 1 24 -3.284914855477184 1 25 -3.1266273980796857 1 26 -3.307539179537809 1 27 -3.5116916714886193 1 28 -3.679637412261502 1 29 -2.932557053531106 1 30 -3.357656824724682 1 31 -3.6407015730831436 1 32 -3.5790642692277883 1 33 -3.2527522208846467 1 34 -3.4428667085037015 1 35 -3.2767437569518965 1 36 -3.409363299452144 1 37 -3.2189540839602593 1 38 -3.3871630149351803 1 39 -2.9927559629866263 1 40 -2.9899349185849067 2 1 7.574965187520714 2 2 -4.00925932826533 2 3 9.372968083831477 2 4 11.67598863354346 2 5 -2.135574340657559 2 6 -3.491457766209022 2 7 1.382538765317129 2 8 1.2107581236445668 2 9 -8.00905115401534 2 10 2.7177105272499045 2 11 -2.904933143309716 2 12 4.052838213373468 2 13 6.464560448020219 2 14 -4.012785337790632 2 15 5.272084833620557 2 16 3.0614356407132437 2 17 0.997345427422875 2 18 0.531792904724151 2 19 2.1844299340768245 2 20 5.858172118069879 2 21 8.939796687462831 2 22 -2.2985164487004823 2 23 6.714629677834186 2 24 -0.28491485547718387 2 25 2.3733726019203143 2 26 1.1924608204621912 2 27 4.488308328511381 2 28 5.320362587738498 2 29 4.067442946468894 2 30 4.642343175275318 2 31 2.3592984269168564 2 32 9.42093573077221 2 33 6.747247779115353 2 34 3.0571332914962985 2 35 3.2232562430481035 2 36 10.090636700547856 2 37 2.2810459160397407 2 38 9.11283698506482 2 39 3.5072440370133737 2 40 6.510065081415093 3 1 5.574965187520714 3 2 0.15740733840133814 3 3 -1.4603652495018569 3 4 7.17598863354346 3 5 9.36442565934244 3 6 11.175208900457646 3 7 6.382538765317129 3 8 4.877424790311235 3 9 1.8242821793179917 3 10 3.0510438605832366 3 11 8.761733523356952 3 12 7.3861715467068 3 13 9.464560448020219 3 14 4.987214662209368 3 15 6.938751500287225 3 16 -2.7718976926200893 3 17 -4.835987905910458 3 18 -2.468207095275849 3 19 0.1844299340768245 3 20 -4.641827881930121 3 21 -3.560203312537169 3 22 -3.2985164487004823 3 23 -3.2853703221658144 3 24 -3.284914855477184 3 25 -3.1266273980796857 3 26 -3.307539179537809 3 27 -3.5116916714886193 3 28 -3.679637412261502 3 29 -2.932557053531106 3 30 -3.357656824724682 3 31 -3.6407015730831436 3 32 -3.5790642692277883 3 33 -3.2527522208846467 3 34 -3.4428667085037015 3 35 -3.2767437569518965 3 36 -3.409363299452144 3 37 -3.2189540839602593 3 38 -3.3871630149351803 3 39 -2.9927559629866263 3 40 -2.9899349185849067 4 1 0.874965187520715 4 2 6.090740671734672 4 3 -0.32703191616852223 4 4 5.97598863354346 4 5 7.664425659342442 4 6 -0.3914577662090224 4 7 9.382538765317129 4 8 6.610758123644565 4 9 6.590948845984661 4 10 -2.982289472750095 4 11 11.195066856690286 4 12 12.252838213373467 4 13 8.56456044802022 4 14 9.887214662209367 4 15 9.472084833620556 4 16 17.161435640713243 4 17 11.197345427422874 4 18 13.43179290472415 4 19 12.084429934076823 4 20 18.158172118069878 4 21 18.43979668746283 4 22 20.901483551299517 4 23 20.914629677834185 4 24 17.515085144522818 4 25 20.673372601920313 4 26 14.692460820462191 4 27 17.68830832851138 4 28 12.720362587738496 4 29 6.267442946468893 4 30 11.042343175275317 4 31 14.359298426916856 4 32 12.62093573077221 4 33 9.147247779115352 4 34 13.757133291496299 4 35 13.723256243048104 4 36 17.39063670054786 4 37 18.98104591603974 4 38 21.81283698506482 4 39 20.207244037013375 4 40 17.010065081415092 5 1 -3.3694792569237286 5 2 -0.9537037727097726 5 3 1.4285236393870342 5 4 5.842655300210128 5 5 4.419981214897998 5 6 3.452986678235421 5 7 -1.1730167902384263 5 8 1.4329803458667882 5 9 3.1020599570957703 5 10 7.106599416138794 5 11 -2.73826647664305 5 12 -3.280495119959866 5 13 -2.146550663090892 5 14 1.9316591066538127 5 15 -3.9501373886016653 5 16 -5.438564359286756 5 17 -5.002654572577125 5 18 -4.968207095275849 5 19 -4.3155700659231755 5 20 -4.641827881930121 5 21 -3.560203312537169 5 22 -3.2985164487004823 5 23 -3.2853703221658144 5 24 -3.284914855477184 5 25 -3.1266273980796857 5 26 -3.307539179537809 5 27 -3.5116916714886193 5 28 -3.679637412261502 5 29 -2.932557053531106 5 30 -3.357656824724682 5 31 -3.6407015730831436 5 32 -3.5790642692277883 5 33 -3.2527522208846467 5 34 -3.4428667085037015 5 35 -3.2767437569518965 5 36 -3.409363299452144 5 37 -3.2189540839602593 5 38 -3.3871630149351803 5 39 -2.9927559629866263 5 40 -2.9899349185849067 6 1 1.8169006713916822 6 2 4.0391277685088625 6 3 -2.610902883910459 6 4 -1.5336887858113784 6 5 3.1870063045037327 6 6 -5.185006153305796 6 7 1.221248442736485 6 8 5.920435542999407 6 9 7.28127142662982 6 10 -1.4274507630726756 6 11 1.07893782443222 6 12 6.278644664986372 6 13 4.706495931891187 6 14 6.583988855757756 6 15 8.497891285233461 6 16 8.69046789877776 6 17 9.416700266132551 6 18 7.064050969240279 6 19 7.4908815469800505 6 20 9.616236634198911 6 21 6.472054751978961 6 22 6.63696742226726 6 23 10.166242581059993 6 24 5.715085144522816 6 25 7.260469376113862 6 26 10.821493078526707 6 27 10.230243812382348 6 28 9.642943232899789 6 29 8.260991333565668 6 30 9.868149626888222 6 31 13.681879072078148 6 32 13.517709924320599 6 33 13.87628003717987 6 34 14.105520388270492 6 35 14.91680463014488 6 36 12.17128186183818 6 37 12.813303980555869 6 38 12.290256339903529 6 39 11.523373069271438 6 40 12.493936049157028 7 1 3.4227912744772375 7 2 8.055958063039018 7 3 2.481663736005391 7 4 9.610771242239112 7 5 6.6470343549946165 7 6 -2.9479795053394575 7 7 -0.7478960172915663 7 8 6.732497254079348 7 9 6.25181841120205 7 10 7.6090148750759905 7 11 1.9428929436468056 7 12 8.661533865547382 7 13 4.573256100194133 7 14 1.4437364013398035 7 15 4.924258746664034 7 16 10.387522597234982 7 17 9.77995412307505 7 18 10.075271165593717 7 19 9.467038629729 7 20 8.792954726765531 7 21 11.831101035288917 7 22 7.918874855647344 7 23 8.58419489522549 7 24 4.280302535827164 7 25 10.525546514963791 7 26 8.51854777698393 7 27 10.48830832851138 7 28 9.537753892086322 7 29 4.850051642121068 7 30 8.25103882744923 7 31 12.663646253003813 7 32 10.464413991641777 7 33 11.225508648680572 7 34 12.861481117583256 7 35 12.331951895222018 7 36 14.242810613591333 7 37 14.694089394300612 7 38 11.395445680716996 7 39 14.702896210926417 7 40 14.531804211849874 8 1 12.074965187520714 8 2 -6.50925932826533 8 3 18.872968083831477 8 4 22.17598863354346 8 5 22.86442565934244 8 6 -10.991457766209022 8 7 -11.617461234682871 8 8 -11.789241876355433 8 9 -11.00905115401534 8 10 -9.782289472750096 8 11 -9.404933143309716 8 12 -8.947161786626532 8 13 -8.035439551979781 8 14 -8.512785337790632 8 15 -7.727915166379443 8 16 -5.438564359286756 8 17 -5.002654572577125 8 18 -4.968207095275849 8 19 -4.3155700659231755 8 20 -4.641827881930121 8 21 -3.560203312537169 8 22 -3.2985164487004823 8 23 -3.2853703221658144 8 24 -3.284914855477184 8 25 -3.1266273980796857 8 26 -3.307539179537809 8 27 -3.5116916714886193 8 28 -3.679637412261502 8 29 -2.932557053531106 8 30 -3.357656824724682 8 31 -3.6407015730831436 8 32 -3.5790642692277883 8 33 -3.2527522208846467 8 34 -3.4428667085037015 8 35 -3.2767437569518965 8 36 -3.409363299452144 8 37 -3.2189540839602593 8 38 -3.3871630149351803 8 39 -2.9927559629866263 8 40 -2.9899349185849067 9 1 1.741631854187382 9 2 3.8240740050680024 9 3 3.539634750498145 9 4 4.842655300210128 9 5 0.8644256593424409 9 6 10.675208900457646 9 7 12.715872098650461 9 8 14.210758123644567 9 9 13.657615512651327 9 10 15.884377193916572 9 11 16.595066856690284 9 12 17.05283821337347 9 13 16.631227114686887 9 14 6.487214662209368 9 15 10.60541816695389 9 16 3.5614356407132437 9 17 4.330678760756209 9 18 4.031792904724151 9 19 -2.3155700659231755 9 20 5.358172118069879 9 21 1.7731300207961649 9 22 2.3681502179661837 9 23 1.3812963445008517 9 24 5.715085144522816 9 25 -3.1266273980796857 9 26 -3.307539179537809 9 27 -3.5116916714886193 9 28 -3.679637412261502 9 29 -2.932557053531106 9 30 -3.357656824724682 9 31 -3.6407015730831436 9 32 -3.5790642692277883 9 33 -3.2527522208846467 9 34 -3.4428667085037015 9 35 -3.2767437569518965 9 36 -3.409363299452144 9 37 -3.2189540839602593 9 38 -3.3871630149351803 9 39 -2.9927559629866263 9 40 -2.9899349185849067 10 1 -5.407793433168941 10 2 -4.7851213972308475 10 3 -0.8166870885823165 10 4 -2.4791837802496435 10 5 -6.101091582036869 10 6 -1.6466301800021252 10 7 6.037711179110232 10 8 5.003861571920428 10 9 5.094397121846727 10 10 4.010813975525766 10 11 7.181273753242007 10 12 5.363183040959674 10 13 4.930077689399528 10 14 2.3148008691059196 10 15 4.720360695689521 10 16 2.9752287441615195 10 17 2.3766557722504604 10 18 5.066275663344841 10 19 4.891326485800963 10 20 4.220241083587121 10 21 6.1294518598766246 10 22 5.46010424095469 10 23 8.576698643351428 10 24 5.542671351419368 10 25 6.769924326058245 10 26 5.589012544600122 10 27 8.350377294028622 10 28 8.25139707049712 10 29 5.653649843020618 10 30 6.228550071827042 10 31 7.01447084070996 10 32 6.59334952387566 10 33 7.298971917046387 10 34 8.039891912185954 10 35 8.274980380979137 10 36 10.107878079858201 10 37 10.125873502246638 10 38 10.647319743685511 10 39 7.5589681749444075 10 40 8.285927150380612 11 1 3.0749651875207142 11 2 3.9907406717346703 11 3 -1.127031916168523 11 4 -4.82401136645654 11 5 -8.13557434065756 11 6 -4.741457766209022 11 7 -1.867461234682871 11 8 -0.2892418763554332 11 9 -1.0090511540153404 11 10 -1.0322894727500955 11 11 0.5950668566902841 11 12 1.8028382133734677 11 13 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5.312386534976742 12 14 6.443736401339802 12 15 5.61991092057708 12 16 6.300566075495853 12 17 5.910388905683744 12 18 6.901358122115456 12 19 8.162690803642043 12 20 7.8364329876350975 12 21 8.3528401657237 12 22 9.13626615999517 12 23 9.453760112616795 12 24 6.975954709740207 12 25 11.960329123659443 12 26 11.562026037853496 12 27 12.40135180677225 12 28 12.102971283390673 12 29 10.676138598642808 12 30 11.729299697014447 12 31 11.489733209525552 12 32 14.073109643815688 12 33 12.225508648680572 12 34 13.339741987148475 12 35 11.810212764787233 12 36 12.851506265765247 12 37 11.085393742126698 12 38 10.482402202456125 12 39 9.31159186310033 12 40 11.010065081415092 13 1 1.690349802905331 13 2 -7.432336251342253 13 3 -0.2039549930915996 13 4 -1.0547805972257702 13 5 1.7105795054962876 13 6 2.777773003021748 13 7 12.074846457624822 13 8 11.672296585183027 13 9 7.99094884598466 13 10 8.679248988788364 13 11 9.90275916438259 13 12 9.514376674911928 13 13 7.5799450634048355 13 14 6.025676200670908 13 15 7.42593098746671 13 16 5.946051025328629 13 17 8.228114658192105 13 18 4.493331366262613 13 19 2.607506857153748 13 20 6.0504798103775705 13 21 4.824412072078216 13 22 11.009175858991824 13 23 8.022321985526494 13 24 4.6381620675997395 13 25 10.488757217304931 13 26 9.923230051231421 13 27 8.565231405434456 13 28 9.781901049276957 13 29 10.836673715699664 13 30 11.642343175275318 13 31 9.28237534999378 13 32 8.497858807695287 13 33 7.131863163730738 13 34 9.480210214573223 13 35 8.415563935355795 13 36 8.667559777470933 13 37 7.011815146808971 13 38 8.766683138910974 13 39 8.545705575474912 13 40 9.702372773722786 14 1 -7.925034812479286 14 2 -5.00925932826533 14 3 -5.127031916168523 14 4 1.6759886335434597 14 5 -6.135574340657559 14 6 -4.991457766209022 14 7 -0.617461234682871 14 8 1.2107581236445668 14 9 0.4909488459846596 14 10 3.7177105272499045 14 11 -4.404933143309716 14 12 -1.9471617866265323 14 13 3.964560448020219 14 14 -3.012785337790632 14 15 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-7.727915166379443 120 16 -5.438564359286756 120 17 -5.002654572577125 120 18 -4.968207095275849 120 19 -4.3155700659231755 120 20 -4.641827881930121 120 21 -3.560203312537169 120 22 -3.2985164487004823 120 23 -3.2853703221658144 120 24 -3.284914855477184 120 25 -3.1266273980796857 120 26 -3.307539179537809 120 27 -3.5116916714886193 120 28 -3.679637412261502 120 29 -2.932557053531106 120 30 -3.357656824724682 120 31 -3.6407015730831436 120 32 -3.5790642692277883 120 33 -3.2527522208846467 120 34 -3.4428667085037015 120 35 -3.2767437569518965 120 36 -3.409363299452144 120 37 -3.2189540839602593 120 38 -3.3871630149351803 120 39 -2.9927559629866263 120 40 -2.9899349185849067 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 2660.0 5 458.0 6 194.0 7 256.0 8 288.0 9 384.0 10 402.0 11 417.0 12 338.0 13 296.0 14 253.0 15 191.0 16 144.0 17 97.0 18 95.0 19 144.0 20 182.0 21 276.0 22 316.0 23 314.0 24 275.0 25 264.0 26 218.0 27 177.0 28 102.0 29 78.0 30 68.0 31 34.0 32 19.0 33 8.0 34 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 97.06967900682251 1.2973940275137008 1.219103008612012 0.4138239570517839 8 95.67210914784164 1.934690225900246 1.8899575039141132 0.5032431223439947 9 94.59671104150353 1.823470186821792 2.5618078084796956 1.0180109631949883 10 42.32620620172394 30.359341766483823 18.817866338296206 8.496585693496025 11 30.971275287805966 31.65306806750866 20.90086062367274 16.47479602101263 12 31.600715403532302 26.648781578359042 20.69081153588196 21.059691482226693 13 20.52543320290665 25.56735606484069 23.1078814980436 30.799329234209054 14 20.382165605095544 32.62934406078892 30.986702424851938 16.001787909263605 15 35.28885909040117 25.868812157782994 24.06972846128059 14.772600290535257 16 32.58464632919879 31.735389428986476 23.231645993965806 12.448318247848922 17 24.564148413053196 27.11220384443451 34.711667411712114 13.611980330800177 18 24.53905464297687 27.99195440831378 30.684992736618614 16.783998212090737 19 25.441933318415757 31.33810695905124 22.555381517117922 20.664578205415083 20 23.019331768912725 33.9814504413901 29.154095429656945 13.845122360040227 21 19.376466644317798 31.657168398703767 30.66264387082356 18.30372108615488 22 18.516035311207958 28.349536261034753 30.964353559056878 22.17007486870041 23 21.0979427549195 28.67844364937388 28.24239713774597 21.981216457960645 24 21.09732931053749 30.36093418259023 28.483629455805122 20.058107051067157 25 26.820266189464263 27.178167990157696 25.780114081198974 20.221451739179063 26 25.215734618401882 27.30023534685644 25.12607867309201 22.35795136164967 27 19.573991031390133 30.15695067264574 24.753363228699552 25.515695067264577 28 22.202327663384064 35.10519247985676 23.936884512085946 18.75559534467323 29 30.686406460296094 29.62090623598026 22.24091520861373 17.451772095109916 30 24.102449390448495 27.245274577787722 31.60720277373896 17.04507325802483 31 26.156941649899395 32.84149340487368 23.429465682986812 17.57209926224011 32 30.61819813755189 27.723549870974978 24.683047234376755 16.975204757096378 33 26.28226617499162 28.640071516370547 24.997206391775617 20.08045591686222 34 20.214549111632586 31.545424069728462 32.964577047714826 15.275449770924126 35 17.566208514917868 31.210191082802545 35.98167393004805 15.241926472231535 36 20.096336955304135 35.43183600313655 27.086367200627308 17.385459840932004 37 22.02480724103252 29.366409654710026 32.21589004358029 16.39289306067717 38 26.326963906581742 29.936305732484076 27.60084925690021 16.13588110403397 39 23.835065370432453 29.802212537713714 23.64510001117443 22.717622080679405 40 20.56095653145603 27.7796401832607 30.986702424851938 20.672700860431334 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 1.0 3 1.0 4 1.5 5 2.0 6 3.5 7 5.0 8 5.0 9 17.5 10 30.0 11 49.0 12 68.0 13 68.0 14 72.0 15 76.0 16 87.0 17 98.0 18 98.0 19 102.0 20 106.0 21 138.5 22 171.0 23 171.0 24 174.5 25 178.0 26 205.5 27 233.0 28 233.0 29 282.0 30 331.0 31 360.5 32 390.0 33 390.0 34 413.0 35 436.0 36 504.5 37 573.0 38 573.0 39 669.0 40 765.0 41 728.0 42 691.0 43 691.0 44 729.0 45 767.0 46 890.5 47 1014.0 48 1014.0 49 919.5 50 825.0 51 729.5 52 634.0 53 634.0 54 639.5 55 645.0 56 592.5 57 540.0 58 540.0 59 370.0 60 200.0 61 157.5 62 115.0 63 115.0 64 74.5 65 34.0 66 25.5 67 17.0 68 17.0 69 10.0 70 3.0 71 2.0 72 1.0 73 1.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0893954631802436 8 0.07822103028271316 9 0.1117443289753045 10 0.1787909263604872 11 0.0223488657950609 12 0.03352329869259135 13 0.0446977315901218 14 0.0 15 0.0 16 0.0 17 0.01117443289753045 18 0.0 19 0.12291876187283494 20 0.0 21 0.0 22 0.0 23 0.05587216448765225 24 0.0 25 0.0893954631802436 26 0.2905352553357917 27 0.3240585540283831 28 0.14526762766789586 29 0.3687562856185049 30 0.0893954631802436 31 0.03352329869259135 32 0.4022795843110962 33 0.0 34 0.0 35 0.0 36 0.2458375237456699 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 8949.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.83607106939323 #Duplication Level Percentage of deduplicated Percentage of total 1 94.0779711317955 77.93049502737736 2 3.844597329016593 6.369426751592357 3 0.822878726561446 2.0449212202480727 4 0.33724537973829755 1.117443289753045 5 0.18885741265344666 0.7822103028271314 6 0.12140833670578711 0.6034193764666443 7 0.16187778227438285 0.9386523633925578 8 0.08093889113719142 0.5363727790814616 9 0.09442870632672333 0.7039892725444183 >10 0.21583704303251047 2.7153871940998995 >50 0.02697963037906381 2.0672700860431332 >100 0.02697963037906381 4.190412336573919 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 257 2.8718292546653257 No Hit AAAGTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 118 1.318583081908593 No Hit AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 100 1.117443289753045 No Hit AAAGTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 85 0.9498267962900884 No Hit AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 36 0.4022795843110962 No Hit AAAGTTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC 21 0.23466309084813944 No Hit AAAGTTGGGACTAATCCTAGCCCTAGCCCTACACAAATAT 20 0.223488657950609 No Hit AAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.2011397921555481 No Hit AAAGTTGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG 17 0.18996535925801766 No Hit AAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.16761649346295676 No Hit AAAGTTGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT 15 0.16761649346295676 No Hit AAAGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA 14 0.1564420605654263 No Hit AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA 13 0.14526762766789586 No Hit AAAGTTGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 12 0.1340931947703654 No Hit AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.1340931947703654 No Hit AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG 10 0.1117443289753045 No Hit AAAGTTGGGTACATGGTTGATCCTGCCAGTAGCATATGCT 10 0.1117443289753045 No Hit AAAGTTGGGAGTGCAGTGCTAAAGTTGGGTCACAGATCCT 10 0.1117443289753045 No Hit AAAGTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGC 10 0.1117443289753045 No Hit AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAG 10 0.1117443289753045 No Hit AAAGTTGGGGTTGGGCGGATTGAACAAAAACTCATCTCAG 9 0.10056989607777406 No Hit AAAGTTGGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCC 9 0.10056989607777406 No Hit AAAGTTGGGGGGGAGGTAGTGACGAAAAATAACAATACAG 9 0.10056989607777406 No Hit AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG 9 0.10056989607777406 No Hit AAAGTTGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT 9 0.10056989607777406 No Hit AAAGTTGGGTACCTGGTTGATCCTGCCAGTACCATATGCT 9 0.10056989607777406 No Hit AAAGTTGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT 9 0.10056989607777406 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.01117443289753045 0.0 0.0 0.0 0.0 12 0.01117443289753045 0.0 0.0 0.0 0.0 13 0.01117443289753045 0.0 0.0 0.0 0.0 14 0.0223488657950609 0.0 0.0 0.0 0.0 15 0.0223488657950609 0.0 0.0 0.0 0.0 16 0.0223488657950609 0.0 0.0 0.0 0.0 17 0.0223488657950609 0.0 0.0 0.0 0.0 18 0.0223488657950609 0.0 0.0 0.0 0.0 19 0.0223488657950609 0.0 0.0 0.0 0.0 20 0.03352329869259135 0.0 0.0 0.0 0.0 21 0.03352329869259135 0.0 0.0 0.0 0.0 22 0.03352329869259135 0.0 0.0 0.0 0.0 23 0.0446977315901218 0.0 0.0 0.0 0.0 24 0.055872164487652254 0.0 0.0 0.0 0.0 25 0.055872164487652254 0.0 0.0 0.0 0.0 26 0.0670465973851827 0.0 0.0 0.0 0.0 27 0.07822103028271316 0.0 0.0 0.0 0.0 28 0.0893954631802436 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAGTAG 35 8.524421E-7 34.41143 26 GATGAGT 30 1.267777E-5 34.21591 28 CTTCTGA 50 3.092282E-10 34.21591 21 CTGAGAT 35 8.9211244E-7 34.215908 24 CCTGCCA 35 8.9211244E-7 34.215908 22 TTCTGAG 35 8.9211244E-7 34.215908 22 GCCAGTA 35 8.9211244E-7 34.215908 25 TCTGAGA 35 8.9211244E-7 34.215908 23 CTGCCAG 35 8.9211244E-7 34.215908 23 TCCTGCC 35 8.9211244E-7 34.215908 21 ATGAGTT 35 8.9211244E-7 34.215908 29 ATCCTGC 35 9.3337985E-7 34.0226 20 GTACCTG 30 1.3188317E-5 34.0226 9 GGGTACC 35 9.3337985E-7 34.0226 7 GAGTTTT 35 9.3337985E-7 34.0226 31 GGGAGGA 20 0.002634216 34.0226 7 TTGGGTG 25 1.8634034E-4 34.0226 5 TGGGGTG 25 1.8634034E-4 34.0226 6 TTGGGGT 55 2.3646862E-11 34.0226 5 TTGGGGC 35 9.3337985E-7 34.0226 5 >>END_MODULE