FastQCFastQC Report
Fri 17 Jun 2016
SRR1526046_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526046_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66467
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT7951.1960822663878317No Hit
ATGCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG6330.9523522951238961No Hit
ATGCTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC6070.9132351392420298No Hit
ATGCTTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC2260.3400183549731446No Hit
ATGCTTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT2250.33851384897768816No Hit
ATGCTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1970.2963876811049092No Hit
ATGCTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1660.24974799524576105No Hit
ATGCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1510.22718040531391515No Hit
ATGCTTGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT1380.20762182737298207No Hit
ATGCTTGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT1370.2061173213775257No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1280.19257676741841817No Hit
ATGCTTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC1200.18054071945476702No Hit
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1180.17753170746385424No Hit
ATGCTTGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG1050.15797312952292114No Hit
ATGCTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA960.14443257556381361No Hit
ATGCTTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC930.13991905757744444No Hit
ATGCTTGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC920.13841455158198807No Hit
ATGCTTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA880.1323965276001625No Hit
ATGCTTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG830.12487399762288053No Hit
ATGCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG790.11885597364105496No Hit
ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT750.11283794965922939No Hit
ATGCTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC740.111333443663773No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.10982893766831661No Hit
ATGCTTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC720.10832443167286022No Hit
ATGCTTGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG700.10531541968194742No Hit
ATGCTTGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG680.10230640769103465No Hit
ATGCTTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC680.10230640769103465No Hit
ATGCTTGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG670.10080190169557826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGAA600.050.38537641
GACAAAT150.008144950.38537639
CGTCTCA204.0004496E-450.38537641
GCATTGA600.050.38537640
CGTAGAG251.9741117E-550.38537239
CGACAAA150.00816947450.3470338
GCGCATT650.050.3470338
GCGTAGA251.983039E-550.3470338
AGCGTAG252.000994E-550.27051537
GGCGCAT650.050.27051537
TGCTTGC402.5120244E-950.23234635
ATGCTCG252.0100219E-550.23234636
TATGCCT252.0100219E-550.23234635
GCTTGCA252.0100219E-550.23234636
GACGACA150.00824352850.23234636
GCGCCTA150.00829317350.15617846
ATATGCC355.0622475E-850.15617834
ACCCGCT150.00829317350.15617834
AAATTCT204.106951E-450.11818744
AGCCGCA150.0083180850.1181844
Produced by FastQC (version 0.11.5)