##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526046_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 66467 Sequences flagged as poor quality 0 Sequence length 56 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.91607865557344 39.0 38.0 40.0 33.0 40.0 2 36.05447816209548 39.0 38.0 40.0 28.0 40.0 3 35.56367821625769 39.0 35.0 40.0 27.0 40.0 4 35.75282471000647 39.0 36.0 40.0 27.0 40.0 5 36.030451201348036 39.0 38.0 40.0 28.0 40.0 6 36.08844990747288 39.0 38.0 40.0 29.0 40.0 7 36.32303248228444 39.0 38.0 40.0 30.0 40.0 8 36.117637323784734 39.0 37.0 40.0 30.0 40.0 9 36.13772247882408 39.0 37.0 40.0 30.0 40.0 10 35.50960627078099 39.0 36.0 40.0 27.0 40.0 11 34.943806701069704 38.0 35.0 40.0 26.0 40.0 12 33.777483563272 38.0 33.0 40.0 20.0 40.0 13 33.16156889885206 38.0 32.0 39.0 17.0 40.0 14 33.89545187837574 38.0 33.0 40.0 21.0 40.0 15 32.64379316051574 38.0 31.0 39.0 14.0 40.0 16 32.167150616095206 37.0 29.0 39.0 16.0 40.0 17 31.117682458964598 36.0 27.0 39.0 13.0 40.0 18 30.477259391878675 35.0 25.0 39.0 12.0 40.0 19 31.727323333383485 36.0 28.0 39.0 15.0 40.0 20 32.32640257571426 37.0 30.0 39.0 18.0 40.0 21 32.650081995576755 38.0 30.0 39.0 17.0 40.0 22 31.863541306212106 37.0 29.0 39.0 14.0 40.0 23 32.57442038906525 38.0 30.0 39.0 17.0 40.0 24 31.975566822633787 37.0 29.0 39.0 14.0 40.0 25 32.394436336828804 37.0 30.0 39.0 16.0 40.0 26 32.85114417680955 38.0 32.0 39.0 16.0 40.0 27 33.048008786315016 38.0 32.0 39.0 17.0 40.0 28 33.07010997938827 38.0 32.0 39.0 18.0 40.0 29 32.58878842132186 38.0 31.0 39.0 15.0 40.0 30 32.39380444431071 37.0 31.0 39.0 14.0 40.0 31 33.14339446642695 38.0 33.0 39.0 16.0 40.0 32 33.00689063745919 38.0 33.0 39.0 15.0 40.0 33 32.798621872508164 38.0 32.0 39.0 14.0 40.0 34 32.63645117125792 37.0 32.0 39.0 11.0 40.0 35 32.579114447771076 38.0 32.0 39.0 10.0 40.0 36 31.652263529270165 36.0 30.0 39.0 4.0 40.0 37 31.42666285525148 36.0 30.0 39.0 4.0 40.0 38 30.872628522424662 36.0 29.0 38.0 4.0 40.0 39 29.945266071885296 35.0 27.0 38.0 4.0 40.0 40 27.601110325424646 34.0 18.0 38.0 4.0 40.0 41 25.668015707042592 31.0 12.0 38.0 4.0 39.0 42 26.61230385003084 32.0 18.0 38.0 4.0 39.0 43 26.635051980682142 32.0 18.0 38.0 4.0 39.0 44 25.76007642890457 31.0 13.0 37.0 4.0 39.0 45 26.68349707373584 32.0 18.0 38.0 4.0 39.0 46 24.733461717844946 30.0 11.0 36.0 4.0 39.0 47 23.744068485112912 28.0 7.0 36.0 4.0 39.0 48 24.388147501767794 29.0 8.0 36.0 4.0 39.0 49 23.34382475514165 28.0 4.0 35.0 4.0 39.0 50 24.032482284441905 29.0 4.0 36.0 4.0 39.0 51 24.149096544149728 30.0 4.0 36.0 4.0 39.0 52 24.01104307400665 30.0 4.0 36.0 4.0 39.0 53 23.819985857643644 30.0 4.0 36.0 4.0 39.0 54 23.48657228399055 29.0 4.0 36.0 4.0 39.0 55 23.26193449380896 29.0 4.0 36.0 4.0 39.0 56 22.819970812583687 29.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.81137915603113 1 2 3.7169087043109386 1 3 4.3783009073944115 1 4 4.095449991717906 1 5 4.077923918309729 1 6 4.083004025177651 1 7 3.826776880324836 1 8 3.9110941030190887 1 9 3.7621879134691767 1 10 4.250566054715129 1 11 1.3288209366131127 1 12 -5.598432313149317 1 13 -17.492427416233756 1 14 -4.484501745388165 1 15 1.6564024057146902 1 16 -0.8676463732439643 1 17 -4.209526031103891 1 18 -2.932097928903623 1 19 0.4526994116406122 1 20 -0.10461192218719262 1 21 6.575479141551327 1 22 7.553673547133794 1 23 6.378896195227895 1 24 7.2450135429698435 1 25 6.389392466064063 1 26 6.639148683211172 1 27 6.164505630636246 1 28 5.927893631151054 1 29 6.814348283671261 1 30 6.069944244268104 1 31 4.477786931224191 1 32 4.5965391941713705 1 33 4.88443923283776 1 34 4.611906302746984 1 35 4.61944316085841 1 36 3.081317149757062 1 37 3.351331698025831 1 38 3.9282090955692155 1 39 4.252404863085626 1 40 -6.743616265609017 1 41 1.5504536080010318 1 42 0.8328889434062781 1 43 -6.760901428765081 1 44 -12.586535829126127 1 45 -0.31321678052780655 1 46 -2.299738479021123 1 47 -6.556231524414315 1 48 -1.7779359665387169 1 49 -11.718366018414224 1 50 -1.1791681678078945 1 51 5.702258181308711 1 52 5.546282409253902 1 53 5.3619751679199545 1 54 5.51364795204103 1 55 5.194136587525591 1 56 7.557108273283582 2 1 3.3765365004130956 2 2 4.3598010787807056 2 3 4.842442172068381 2 4 4.82722467850887 2 5 4.2900182262903925 2 6 4.531756121458599 2 7 4.105656233190572 2 8 4.172216723123661 2 9 4.299131782463945 2 10 4.768766705795329 2 11 4.645975913430718 2 12 1.8361143969226958 2 13 3.3057377213809254 2 14 5.356279550853323 2 15 6.293671814810086 2 16 5.737956862427339 2 17 7.8507482117687495 2 18 6.3048558049426795 2 19 6.988558146966646 2 20 6.04415003894529 2 21 7.570349418753658 2 22 8.411225090983063 2 23 7.760961895139111 2 24 8.403739004151653 2 25 7.7489663044778005 2 26 6.76107824817122 2 27 7.145861061237017 2 28 6.468191549667377 2 29 7.239917978847348 2 30 7.31903174550121 2 31 5.551773317729076 2 32 5.687591773830043 2 33 6.116559189432412 2 34 5.1754852708835095 2 35 6.353190817956168 2 36 7.250499353564898 2 37 6.725505516709859 2 38 6.99390900678555 2 39 7.205152224237839 2 40 5.564660344827999 2 41 10.739464163392174 2 42 9.472427268354487 2 43 8.152386526251195 2 44 -2.8119476866776694 2 45 8.525295599860865 2 46 7.334768771644757 2 47 4.922608369045289 2 48 7.534286584511893 2 49 0.5135566411497479 2 50 7.919283050501271 2 51 10.08353469309721 2 52 10.809476648180606 2 53 11.009405374095351 2 54 10.217011866414119 2 55 10.391137672659262 2 56 10.24064383607336 3 1 3.089754305374022 3 2 4.4322611535343555 3 3 4.764800310022849 3 4 4.687146526962827 3 5 4.027446021057635 3 6 4.466516569980513 3 7 4.0298832124634245 3 8 4.196936396925899 3 9 4.204840243218278 3 10 4.588678360569375 3 11 5.27416143840523 3 12 5.466083815882939 3 13 6.5168318430255034 3 14 5.805820835256995 3 15 0.9422446996215008 3 16 6.9223775216186425 3 17 8.559888544762298 3 18 9.001593827368776 3 19 7.547681490493659 3 20 6.556977086176005 3 21 7.642384756844045 3 22 8.564724921088395 3 23 7.715515072169353 3 24 8.726793710233878 3 25 7.591304055072431 3 26 6.7475716222792705 3 27 6.711100612715104 3 28 6.527654682525007 3 29 6.755208498725022 3 30 7.241389883455678 3 31 5.87533770780723 3 32 5.807112670873877 3 33 5.588441622324016 3 34 5.564713949103012 3 35 5.576432408170241 3 36 6.694519061346071 3 37 7.347150097070035 3 38 7.63041937036013 3 39 8.766144409083235 3 40 10.64586999484499 3 41 13.106606535121703 3 42 11.807799337671511 3 43 11.752539431449971 3 44 12.181086632044721 3 45 11.815815477536706 3 46 12.569138462436467 3 47 13.312346731260714 3 48 13.129172755086138 3 49 13.76669969245794 3 50 12.033197423589954 3 51 12.00572293638637 3 52 11.200404273053188 3 53 11.686945299341701 3 54 12.032166470559552 3 55 12.038521294813528 3 56 11.36415725775192 4 1 3.2462098263155212 4 2 4.487384033423595 4 3 4.975426824387682 4 4 4.254730466042091 4 5 4.591741625072871 4 6 4.829964129689756 4 7 4.373254621255391 4 8 4.390342419771173 4 9 4.286356338753066 4 10 4.788401448142928 4 11 5.129042019921329 4 12 6.163903130524687 4 13 6.797292209934465 4 14 5.974045134322417 4 15 6.873968479476122 4 16 8.222394828645292 4 17 9.590695052204321 4 18 10.159953121242093 4 19 8.322863214273518 4 20 7.929856975411035 4 21 7.612962811534246 4 22 8.636981757363923 4 23 7.912209430078661 4 24 8.457755980915778 4 25 7.514806997266962 4 26 7.023532162258501 4 27 6.539241838052586 4 28 6.15832273375381 4 29 7.382934355422844 4 30 6.470707986605557 4 31 5.50401545097095 4 32 5.349428351040025 4 33 6.031761110036026 4 34 5.914488957756529 4 35 5.944033654276161 4 36 6.642165305721292 4 37 6.63461980977339 4 38 6.979761703347329 4 39 7.9450990646734105 4 40 4.74728917103052 4 41 11.732947829687294 4 42 10.588692380246798 4 43 11.102227904970224 4 44 12.145953367939494 4 45 11.018307689374712 4 46 12.555552446514003 4 47 14.027126932022224 4 48 12.72583249894214 4 49 13.44513513724852 4 50 13.224007392437308 4 51 12.48720441786785 4 52 11.654713722689735 4 53 13.124813073658068 4 54 12.479380039268442 4 55 11.155949470652917 4 56 11.05072700506933 5 1 3.2641079042559227 5 2 4.694490363876774 5 3 5.0862538390235486 5 4 4.814155611519084 5 5 4.77045790086197 5 6 4.747439001846345 5 7 4.4117839811566455 5 8 4.276076858141835 5 9 4.426014247411153 5 10 4.454157046802777 5 11 5.217033555805649 5 12 6.044700168084198 5 13 6.60191220640775 5 14 6.409594420162655 5 15 7.304404028761962 5 16 8.169582273540371 5 17 9.008875267333924 5 18 9.849602213113013 5 19 8.4522936498228 5 20 7.97014969274484 5 21 8.064117810652569 5 22 8.748337779229555 5 23 8.018275379646639 5 24 8.268547728402993 5 25 8.01233829686139 5 26 6.974510825633569 5 27 6.816970633679464 5 28 6.838626031232206 5 29 6.814956926397979 5 30 7.10057926151298 5 31 4.927573906269846 5 32 5.977448100643265 5 33 5.51174700317657 5 34 5.591618211856332 5 35 6.054419829553886 5 36 7.286936950933772 5 37 7.550154988754173 5 38 7.835695401107863 5 39 9.467052864708673 5 40 10.82232002978559 5 41 13.154125752452241 5 42 12.167338121738595 5 43 11.600023708179531 5 44 12.459213815832282 5 45 11.705752584454945 5 46 12.871898769913752 5 47 14.095016193175457 5 48 13.390353747399203 5 49 14.457125967789871 5 50 13.267474153149706 5 51 12.86456290843389 5 52 12.627029010998676 5 53 13.108678353150225 5 54 12.953899524368076 5 55 13.030310253583615 5 56 12.54270372875122 6 1 2.464398180017895 6 2 3.5244976207708234 6 3 3.9681478883704244 6 4 3.56143427625058 6 5 3.9074317760433956 6 6 3.962243065707014 6 7 3.3083732409534505 6 8 3.146722721712223 6 9 3.4153103286883635 6 10 3.8336926055836145 6 11 4.330785370029162 6 12 5.030186379985508 6 13 5.757572583766244 6 14 5.430820835256995 6 15 6.295877178253978 6 16 7.0416795159206345 6 17 8.148207633081388 6 18 8.643687844462793 6 19 7.824390892203063 6 20 6.053059707258626 6 21 6.348225212684497 6 22 7.429397285760761 6 23 5.996141852796136 6 24 5.946523054963222 6 25 5.324566163334534 6 26 4.008255382963032 6 27 5.099989501603989 6 28 4.6230962779666065 6 29 5.165464908981438 6 30 5.45435284641864 6 31 3.7798961123656305 6 32 4.192796431557639 6 33 3.5282564371388325 6 34 3.446480330869395 6 35 3.1180990748369055 6 36 3.9213709131979257 6 37 4.126352376272315 6 38 4.4238666638074235 6 39 4.550688568627397 6 40 0.28440275837775175 6 41 9.116934170449337 6 42 7.579166859039031 6 43 8.393565072475614 6 44 8.90188435284244 6 45 8.177994964716195 6 46 10.878611396909402 6 47 11.119326788240773 6 48 10.584657085570463 6 49 10.337568638326886 6 50 9.82806921846175 6 51 8.477589033252467 6 52 8.54121623886515 6 53 8.940862678259077 6 54 8.170699234092314 6 55 8.18524494153717 6 56 7.256963525558188 7 1 2.877859718479435 7 2 3.7744976207708234 7 3 4.121994042216578 7 4 4.18643427625058 7 5 3.974739468351089 7 6 4.096858450322394 7 7 3.664142471722684 7 8 3.723645798635303 7 9 3.4537718671499036 7 10 3.776000297891308 7 11 4.580785370029162 7 12 5.087878687677815 7 13 6.046034122227784 7 14 5.017359296795455 7 15 6.6804925628693645 7 16 6.714756438997554 7 17 8.18666917154292 7 18 9.143687844462793 7 19 6.853237046049216 7 20 7.28382893802786 7 21 5.9924559819152705 7 22 7.10247420883768 7 23 6.428834160488442 7 24 6.879215362655529 7 25 5.939950778719155 7 26 5.671716921424572 7 27 5.5519125785270695 7 28 4.767327047197373 7 29 5.5885418320583575 7 30 5.800506692572487 7 31 4.770280727750251 7 32 5.288950277711486 7 33 4.374410283292679 7 34 4.648403407792475 7 35 4.906560613298446 7 36 6.190601682428692 7 37 5.770583145503082 7 38 5.827712817653584 7 39 7.310303953242784 7 40 7.284402758377748 7 41 10.655395708910877 7 42 10.415705320577494 7 43 10.36471891862946 7 44 11.27688435284244 7 45 8.745302657023888 7 46 10.782457550755556 7 47 11.648172942086926 7 48 10.79619554710893 7 49 10.702953253711499 7 50 10.299223064615596 7 51 10.169896725560157 7 52 10.243139315788227 7 53 9.642785755182153 7 54 10.257237695630774 7 55 10.069860326152558 7 56 9.516578910173575 8 1 3.195952340143485 8 2 4.309623209467844 8 3 4.78898776278173 8 4 4.598522188338492 8 5 4.372306187346375 8 6 4.4959950280304 8 7 4.187101655396155 8 8 3.9922878708488057 8 9 4.084658837322586 8 10 4.544838602443896 8 11 4.965204522305456 8 12 5.921866128808112 8 13 6.7524705429499186 8 14 5.662963692399849 8 15 7.309024744972977 8 16 7.8307297513994385 8 17 8.977877962751716 8 18 9.781639178842699 8 19 7.766502352171663 8 20 7.230257509456429 8 21 7.412981884584028 8 22 8.618957725321199 8 23 7.607994286077137 8 24 7.728116461556631 8 25 7.358121893318842 8 26 6.658569354705573 8 27 6.875499705685623 8 28 6.046173201043526 8 29 6.670174485119585 8 30 7.132924274990071 8 31 5.341513066839735 8 32 5.105865505340994 8 33 6.010399294281687 8 34 5.087767615013821 8 35 5.181874585040987 8 36 6.15351377034078 8 37 6.41442145005567 8 38 6.39364688358765 8 39 6.68942483236366 8 40 3.3960589593196673 8 41 11.832985975786858 8 42 10.037958067830239 8 43 10.029750315803714 8 44 9.736264258650916 8 45 9.271597790461879 8 46 11.369780941650372 8 47 12.014538719166989 8 48 11.858008733922112 8 49 11.521045875375549 8 50 10.32061238957635 8 51 10.374959519908668 8 52 9.926027855819626 8 53 10.68203222300005 8 54 10.200722781973003 8 55 10.626767704488508 8 56 9.635888172339978 9 1 3.2720904877102015 9 2 4.61103608230929 9 3 5.172818218040753 9 4 4.2963243861406895 9 5 4.614849358460972 9 6 4.258946362410306 9 7 4.377054559634772 9 8 4.095898545888048 9 9 4.372727911105947 9 10 4.405121177012191 9 11 4.911829326073118 9 12 5.689527039326165 9 13 6.353726429920091 9 14 5.706919736355893 9 15 6.5554925628693645 9 16 7.3260201752612915 9 17 8.494361479235234 9 18 9.178028503803457 9 19 7.361478804290975 9 20 6.845642124841049 9 21 7.482840597299884 9 22 8.195880802244279 9 23 6.845042951697231 9 24 7.810534043974215 9 25 7.062203525971899 9 26 6.3296839543916015 9 27 6.385703787318274 9 28 6.2219973768677015 9 29 6.445684689201212 9 30 6.528528670594465 9 31 5.266159848629368 9 32 4.955159068920274 9 33 5.790619074501468 9 34 5.567359451748519 9 35 5.825516657254489 9 36 6.637030253857269 9 37 6.291187541107476 9 38 7.492547982488745 9 39 7.936677579616411 9 40 3.277534626509617 9 41 10.560615489130658 9 42 11.417078946951122 9 43 11.70949913840968 9 44 12.1601261110842 9 45 10.841456503177735 9 46 13.23163337493138 9 47 14.171524590438576 9 48 13.418448294361674 9 49 13.734546660304908 9 50 14.012135152527684 9 51 13.827863758527194 9 52 13.303578876227789 9 53 13.52740113979754 9 54 12.773721212114289 9 55 12.539640545932777 9 56 11.208886602481265 10 1 3.2571768299394037 10 2 4.595337495182129 10 3 4.799191844414381 10 4 4.649542596501753 10 5 4.8927143506116835 10 6 4.577627681091627 10 7 3.9013873696818706 10 8 4.247389911665131 10 9 3.747924143445033 10 10 4.075450847341855 10 11 4.985612685570764 10 12 5.707580414522397 10 13 6.589205236827471 10 14 5.8262289985223035 10 15 7.849841071503587 10 16 7.442974649358625 10 17 9.426857554588448 10 18 9.506128974761065 10 19 8.715481944008403 10 20 6.873114652313575 10 21 7.239512496828922 10 22 7.874059766137524 10 23 6.863096326893462 10 24 7.809749114617851 10 25 6.53159128107394 10 26 6.097344864909655 10 27 6.018356848542762 10 28 5.770662996961892 10 29 6.108949995323666 10 30 5.888026315806396 10 31 4.4333498015336055 10 32 4.503824689014465 10 33 4.6124401106082225 10 34 4.240828839503617 10 35 4.039017442183841 10 36 5.857595402993844 10 37 5.853196960259751 10 38 6.526299944812138 10 39 8.097588097669782 10 40 9.447079367482932 10 41 11.047271690072574 10 42 11.344080516809832 10 43 10.856280928048616 10 44 11.307692830079493 10 45 10.302210035359838 10 46 12.328964615119762 10 47 12.300253004881274 10 48 11.674335264534356 10 49 12.123086691702078 10 50 11.963469532433493 10 51 11.456592172969891 10 52 10.987252345615545 10 53 11.477950590346992 10 54 10.231335026870962 10 55 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-7.961154968703539 115 16 1.6115704458861906 115 17 2.860607173839135 115 18 3.878044905312393 115 19 2.7823415236611595 115 20 2.0319632663860716 115 21 6.133816487081745 115 22 6.967571797815868 115 23 5.699069522141713 115 24 6.603382986076888 115 25 5.880966852197911 115 26 5.219793844501492 115 27 5.353720844887572 115 28 4.553635887610692 115 29 5.110929891759849 115 30 4.584949861344011 115 31 3.890401278840951 115 32 3.6450065348871448 115 33 3.362211661019426 115 34 3.691026829147237 115 35 3.3923528061801917 115 36 4.893385838571064 115 37 5.364010240795849 115 38 5.444819591476772 115 39 6.707548499741062 115 40 7.674615157918506 115 41 9.222416374812138 115 42 7.759706468683117 115 43 6.514977242395247 115 44 -0.388012317651242 115 45 5.596910004899893 115 46 3.410901867632706 115 47 -6.915403406937186 115 48 1.3015055356278715 115 49 -2.915445138940626 115 50 1.944888965878512 115 51 3.860338746226059 115 52 3.1713827141808792 115 53 3.6709143430122353 115 54 4.15046387243904 115 55 3.727437823282294 115 56 7.080298772400898 116 1 3.3233725389922526 116 2 4.046933518206721 116 3 4.458532503755045 116 4 4.391562481378784 116 5 4.19909844271006 116 6 4.000704604168547 116 7 3.686578369158582 116 8 3.5665945165840185 116 9 3.8672334056114437 116 10 4.077282349173359 116 11 3.8916828059265995 116 12 4.312237662036786 116 13 4.763982840176499 116 14 4.318641348077506 116 15 -2.8163023089255077 116 16 4.897448746689868 116 17 5.866156351030103 116 18 5.980226306001253 116 19 5.449390892203063 116 20 5.325495604694524 116 21 7.123866238325526 116 22 7.987089593453067 116 23 6.37434698100126 116 24 7.8599845934247625 116 25 6.2893097530781255 116 26 6.4890246137322585 116 27 5.6897330913475805 116 28 6.007711662581986 116 29 6.562900806417328 116 30 6.755634897700691 116 31 6.414511496981017 116 32 5.574206687967894 116 33 6.05069233457473 116 34 5.843916228305293 116 35 5.332842664580497 116 36 7.322011938838955 116 37 7.151993401913337 116 38 8.225148715089475 116 39 9.130816773755601 116 40 10.319659168634164 116 41 9.89578032429549 116 42 8.50865403852621 116 43 3.0281804570910005 116 44 1.7993202502783383 116 45 6.238892400613633 116 46 3.5100216533196544 116 47 -5.540929622015639 116 48 2.1455545214679006 116 49 -0.5310211052628588 116 50 2.924223064615596 116 51 6.525665956329391 116 52 5.93544700809592 116 53 4.655606268002668 116 54 5.8469812853743655 116 55 5.307039813332043 116 56 8.465296858891524 117 1 2.243750406738542 117 2 3.2534112510002444 117 3 3.262850992284058 117 4 3.288998378814682 117 5 3.419746215989413 117 6 3.332013646003908 117 7 2.885633699792862 117 8 3.3972287945867166 117 9 3.3948987227504404 117 10 3.674448341076193 117 11 1.837026935481255 117 12 -5.779530219204776 117 13 -7.545058995181126 117 14 -0.2818984629886181 117 15 -6.155708516752767 117 16 -7.547895382864791 117 17 1.0355220730274048 117 18 -3.1358330732160162 117 19 0.21727213376851395 117 20 0.4307587625892637 117 21 5.492287290957101 117 22 5.903418878203407 117 23 5.002552109206391 117 24 6.057690396393724 117 25 5.285261170082176 117 26 4.570671037483951 117 27 4.626305291077674 117 28 4.7654714466575605 117 29 5.95696288468994 117 30 4.792072144664257 117 31 4.150678838411519 117 32 4.6389840159031195 117 33 4.381326612577428 117 34 3.858086268791123 117 35 3.558888548521118 117 36 4.419177930741789 117 37 4.7808732939511245 117 38 5.092895003888401 117 39 5.705715559180707 117 40 7.583491827203662 117 41 7.263526613094413 117 42 6.4452262382563035 117 43 -3.4711447790763437 117 44 -1.4982505999240878 117 45 4.8158154775367095 117 46 -1.889438535614218 117 47 -0.555099662501469 117 48 0.8830713905637175 117 49 -2.5802788650469353 117 50 0.9849518095548682 117 51 5.259134242429255 117 52 5.887538775977163 117 53 4.5017601141565144 117 54 4.987332162567348 117 55 4.543881918595201 117 56 6.760843417595975 118 1 2.637475103094822 118 2 3.8225745438477503 118 3 4.246994042216578 118 4 4.013357353173653 118 5 3.6285856221972423 118 6 3.712243065707014 118 7 3.077604010184224 118 8 3.1178765678660696 118 9 3.444156482534517 118 10 4.199077220968235 118 11 1.8788622931060885 118 12 -4.815967466168342 118 13 -4.492427416233756 118 14 -6.174948395512235 118 15 -6.367584360207559 118 16 -7.410243561002442 118 17 0.5712845561583073 118 18 -5.36592754015259 118 19 0.353237046049216 118 20 -0.4565556773567572 118 21 5.482840597299884 118 22 5.929397285760761 118 23 5.784603391257669 118 24 5.744599978040149 118 25 5.872643086411461 118 26 6.2005630752707255 118 27 5.792297193911683 118 28 5.892327047197373 118 29 5.703926447442971 118 30 6.03127592334172 118 31 4.952973035442557 118 32 5.990873354634559 118 33 5.076333360215752 118 34 6.081095715484782 118 35 5.531560613298446 118 36 7.2194478362748455 118 37 7.837890837810775 118 38 8.116174356115117 118 39 9.733380876319703 118 40 11.697864296839288 118 41 10.126549555064724 118 42 9.77147455134672 118 43 2.605103534014077 118 44 3.5557305066885974 118 45 9.360687272408502 118 46 2.272842166140169 118 47 -0.41913475022076696 118 48 1.9211955471089261 118 49 1.1933378690961156 118 50 2.424223064615596 118 51 8.141050571714004 118 52 8.012370085018997 118 53 7.662016524412923 118 54 9.738006926400004 118 55 9.877552633844864 118 56 12.651194294788958 119 1 2.3042374452302994 119 2 3.3400831546399417 119 3 3.8994050640305886 119 4 3.045217284287318 119 5 3.304532809338454 119 6 3.54777693482297 119 7 3.169739486648055 119 8 3.0099546390486225 119 9 3.3035135433841134 119 10 3.083979631990047 119 11 1.1004466788695737 119 12 -5.603567925410591 119 13 -2.1342184610098762 119 14 -4.761343343847482 119 15 -5.8119012373602565 119 16 -7.746638509337689 119 17 1.0546370245854035 119 18 -3.9279252439413384 119 19 0.4987594341089192 119 20 0.897634908177114 119 21 4.297995591559356 119 22 5.937721051547211 119 23 4.430412805723805 119 24 4.633233732345545 119 25 4.701862374585975 119 26 4.503375934053732 119 27 5.040288009066675 119 28 4.598412007013675 119 29 4.857198548476266 119 30 5.256591652388789 119 31 4.338162472870803 119 32 4.077842355782664 119 33 4.392062407288083 119 34 3.467146232132311 119 35 3.556531910657803 119 36 4.759057480362102 119 37 5.57296546467645 119 38 5.817953919834984 119 39 7.513518648994793 119 40 9.301480829560301 119 41 8.177640255409155 119 42 7.610452737339834 119 43 -0.5745749964107212 119 44 1.6418384286174117 119 45 7.596910004899893 119 46 -0.9174563413225165 119 47 -0.4079407203700214 119 48 -3.6835690912378 119 49 -2.1244003628212234 119 50 0.7956352345352293 119 51 6.293174567121582 119 52 6.843024505225657 119 53 5.90972031316149 119 54 5.090762379901726 119 55 7.040870659103188 119 56 8.274328623147166 120 1 2.043928818870569 120 2 3.178180801996376 120 3 3.414203950951915 120 4 3.498037926837277 120 5 3.4783248660042787 120 6 3.187797172617053 120 7 2.7806678954515007 120 8 3.163508901633996 120 9 3.177533275233344 120 10 3.2691554478261224 120 11 0.3273629449965654 120 12 -6.031091325359995 120 13 -9.3398850433524 120 14 -6.228077469827753 120 15 -0.9555895753314161 120 16 -7.535406533623043 120 17 0.8568125874490526 120 18 -5.197087905211259 120 19 -0.2950680386965452 120 20 -0.1547303840060401 120 21 4.2481600236362596 120 22 5.731548524352679 120 23 4.22854080977136 120 24 4.90366125574549 120 25 4.3019129690711715 120 26 4.931658251281153 120 27 4.845752213468398 120 28 4.876681675619796 120 29 4.469897764261749 120 30 5.653505388791519 120 31 4.878005629966676 120 32 3.948826418519829 120 33 5.000714064257476 120 34 3.9523473452109847 120 35 4.565132973141992 120 36 5.347218370825047 120 37 5.130263719166706 120 38 4.860959232255929 120 39 6.4824030405961075 120 40 6.488933397230422 120 41 -4.4034374071256295 120 42 -2.324027404324724 120 43 -7.596330625047202 120 44 -14.602026990051952 120 45 -3.9694952569265674 120 46 -7.468683257588644 120 47 -6.118287292593648 120 48 -5.645623227336966 120 49 -13.91063866284652 120 50 -3.7902489040936587 120 51 0.9278823839695463 120 52 0.7587846873658037 120 53 1.5469578542695075 120 54 0.06167055091108864 120 55 0.23641834440548948 120 56 3.223228193094041 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2718.0 5 11.0 6 47.0 7 280.0 8 478.0 9 394.0 10 273.0 11 230.0 12 188.0 13 175.0 14 219.0 15 238.0 16 284.0 17 372.0 18 465.0 19 570.0 20 776.0 21 945.0 22 1124.0 23 1217.0 24 1572.0 25 1692.0 26 1983.0 27 2169.0 28 2127.0 29 2239.0 30 2529.0 31 2928.0 32 3407.0 33 3718.0 34 4154.0 35 4515.0 36 4940.0 37 5417.0 38 5878.0 39 6192.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.67502670498142 0.22567589931845877 0.0737207937773632 0.025576601922758662 8 99.48846796154483 0.32045977703221146 0.10230640769103465 0.08876585373192712 9 96.96842041915536 1.677524184933877 0.8891630433147276 0.4648923525960251 10 48.36084071795026 26.834368934960207 16.320881038710937 8.483909308378594 11 33.65429461236403 25.86697157988175 17.580152556907937 22.898581250846284 12 32.49432048986715 22.772202747227947 19.791776370228835 24.941700392676065 13 25.350926023440202 15.868024734078565 22.394571742368395 36.38647750011284 14 23.89606872583387 30.896535122692466 22.733085591346082 22.474310560127584 15 33.391309608433275 21.090760598315157 21.429543701507885 24.088386091743676 16 30.92395477333976 29.201607925204375 21.547402177022327 18.32703512443354 17 27.603280909022498 21.097148017157046 32.6345097449018 18.665061328918657 18 25.147817714053588 20.229587614906645 24.737087577293995 29.885507093745765 19 25.685215009256613 23.070786736705852 20.837158897635426 30.406839356402116 20 23.089090416076647 24.645235154108043 31.13795908529421 21.127715344521107 21 22.226067070877278 22.567589931845877 26.592143469691727 28.614199527585114 22 20.471813080175124 22.218544540899995 33.76412355003235 23.545518828892533 23 20.906615312862023 22.973806550619106 31.89402259768005 24.225555538838822 24 20.843552974103556 24.03509035917962 25.07034623892141 30.051010427795415 25 24.492160462246833 22.65340515814499 30.550121881489062 22.304312498119113 26 33.95959413482672 22.700448619516457 21.720410682564058 21.619546563092765 27 22.21536144578313 29.521084337349397 21.048192771084338 27.21536144578313 28 27.64612954186414 28.8783570300158 21.569246972090575 21.90626645602949 29 34.18395031954661 26.01290244784758 19.29639454962016 20.506752682985653 30 31.098194521826862 22.42165971479769 27.729675194627234 18.750470568748213 31 31.4168346925878 29.248547955099465 20.745433205934575 18.589184146378162 32 32.77696672335686 27.22935448429481 21.881913820869016 18.111764971479314 33 30.353806280513528 27.805738050750406 21.01289855945995 20.827557109276114 34 22.880193018170853 28.25152680389052 30.56322099072608 18.305059187212546 35 21.62345492915285 28.112752487187215 33.387096774193544 16.876695809466387 36 27.196085811065114 29.323296951449002 25.24952954459917 18.231087692886714 37 27.13213349009677 26.964818666907842 27.13967019384402 18.763377649151366 38 29.242280249204267 28.157668461782144 24.38641746240063 18.213633826612963 39 27.45969564562302 26.95946963989754 20.87238210034654 24.70845261413289 40 22.796015670078045 21.452215372468036 25.369734884144673 30.38203407330924 41 19.34914304849463 19.50592103260373 22.215288136625162 38.929647782276476 42 21.762033417354687 31.335939504626204 22.210315004603565 24.69171207341554 43 17.979630328178047 27.295360241418333 27.767634854771785 26.95737457563184 44 20.553019079007047 28.26928310101896 24.352190608653583 26.825507211320414 45 24.99396718146718 26.29253137065637 27.040600868725868 21.67290057915058 46 20.075494488902308 31.92359957723086 22.15914238260607 25.84176355126076 47 16.819983659636275 28.642539413562503 22.988470965594455 31.549005961206767 48 24.016500805468148 28.00620285753 23.477514641453755 24.499781695548094 49 19.595919968615146 29.01288589793886 22.29682831879772 29.094365814648278 50 22.312354874702212 30.412231235487468 23.7206356864992 23.55477820331112 51 20.080327349046488 31.120808105210706 25.467695420435156 23.331169125307643 52 20.218892272439472 33.68408357704948 26.099737691078484 19.997286459432566 53 20.752154294251692 30.799993963448678 26.450658738662604 21.997193003637022 54 19.90345451802685 27.269573087946902 28.041936943732086 24.785035450294163 55 18.777450758433325 27.626594219304202 28.96988906497623 24.62606595728624 56 20.725763862693324 26.31459826480573 29.167861184458694 23.791776688042248 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 2.0 5 3.0 6 4.0 7 5.0 8 49.5 9 94.0 10 152.5 11 211.0 12 230.0 13 236.0 14 223.0 15 219.0 16 215.0 17 204.0 18 193.0 19 191.5 20 190.0 21 209.5 22 240.5 23 252.0 24 297.0 25 342.0 26 393.0 27 444.0 28 496.0 29 632.0 30 716.0 31 912.5 32 1109.0 33 1228.0 34 1347.0 35 1393.5 36 1440.0 37 1422.5 38 1504.5 39 1604.0 40 1808.0 41 2012.0 42 2454.5 43 2897.0 44 4147.0 45 5397.0 46 5420.0 47 4725.5 48 4008.0 49 3946.0 50 3884.0 51 3909.5 52 3935.0 53 4133.0 54 4071.0 55 3811.0 56 3775.0 57 3739.0 58 3898.5 59 4058.0 60 3632.0 61 3206.0 62 2807.0 63 2156.0 64 1904.0 65 1605.5 66 1307.0 67 1180.0 68 1053.0 69 892.0 70 731.0 71 664.0 72 439.0 73 281.0 74 217.0 75 153.0 76 157.5 77 162.0 78 199.5 79 159.5 80 82.0 81 46.0 82 10.0 83 5.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5235680864188244 16 0.06920727579099403 17 0.03309913190004062 18 0.0 19 0.04212616787277897 20 0.1278830096137933 21 0.0 22 0.0 23 0.0 24 0.015045059954563919 25 0.013540553959107528 26 0.06168474581371207 27 0.10080190169557826 28 0.003009011990912784 29 0.18505423744113622 30 0.08726134773647073 31 0.013540553959107528 32 0.036108143890953404 33 0.15496411753200837 34 0.22868491130937155 35 0.19107226142296177 36 0.06318925180916846 37 0.188063249432049 38 0.264793055200325 39 0.14593708155927002 40 0.9162441512329428 41 1.1569651105059653 42 0.32196428302766783 43 0.28886515112762723 44 0.4829464245415018 45 0.2452344772593919 46 0.3565679209231649 47 0.5611807363052341 48 0.06920727579099403 49 0.29036965712308366 50 0.21815336934117685 51 0.3580724269186213 52 0.2000992973957001 53 0.30691922307310393 54 0.26629756119578135 55 0.3189552710367551 56 0.28886515112762723 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 66467.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.8020521461778 #Duplication Level Percentage of deduplicated Percentage of total 1 88.88440288992776 62.93198128394542 2 6.568210794730132 9.300856063911414 3 1.8657033574160646 3.962868792032136 4 0.8478538036549086 2.4011915687484016 5 0.40373990650233743 1.4292806956835724 6 0.27836804079898003 1.182541712428724 7 0.1827454313642159 0.9057126092647478 8 0.1274968125796855 0.7221628778190681 9 0.13812154696132595 0.8801360073419893 >10 0.6119847003824904 7.707584214723095 >50 0.06162345941351466 2.9398047151217894 >100 0.02337441563960901 2.5742097582258863 >500 0.006374840628984275 3.0616697007537574 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATGCTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 795 1.1960822663878317 No Hit ATGCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 633 0.9523522951238961 No Hit ATGCTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 607 0.9132351392420298 No Hit ATGCTTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 226 0.3400183549731446 No Hit ATGCTTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 225 0.33851384897768816 No Hit ATGCTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 197 0.2963876811049092 No Hit ATGCTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 166 0.24974799524576105 No Hit ATGCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 151 0.22718040531391515 No Hit ATGCTTGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT 138 0.20762182737298207 No Hit ATGCTTGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 137 0.2061173213775257 No Hit ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 128 0.19257676741841817 No Hit ATGCTTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 120 0.18054071945476702 No Hit ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 118 0.17753170746385424 No Hit ATGCTTGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG 105 0.15797312952292114 No Hit ATGCTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 96 0.14443257556381361 No Hit ATGCTTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC 93 0.13991905757744444 No Hit ATGCTTGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 92 0.13841455158198807 No Hit ATGCTTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 88 0.1323965276001625 No Hit ATGCTTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG 83 0.12487399762288053 No Hit ATGCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 79 0.11885597364105496 No Hit ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 75 0.11283794965922939 No Hit ATGCTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 74 0.111333443663773 No Hit ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 73 0.10982893766831661 No Hit ATGCTTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC 72 0.10832443167286022 No Hit ATGCTTGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG 70 0.10531541968194742 No Hit ATGCTTGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG 68 0.10230640769103465 No Hit ATGCTTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 68 0.10230640769103465 No Hit ATGCTTGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG 67 0.10080190169557826 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.007522529977281959 0.0 0.0 0.0 0.0 11 0.012036047963651136 0.0 0.0 0.0 0.0 12 0.01654956595002031 0.0 0.0 0.0 0.0 13 0.021063083936389487 0.0 0.0 0.0 0.0 14 0.05265770984097372 0.0 0.0 0.0 0.0 15 0.05867573382279928 0.0 0.0 0.0 0.0 16 0.06770276979553763 0.0 0.0 0.0 0.0 17 0.0752252997728196 0.0 0.0 0.0 0.0 18 0.07672980576827598 0.0 0.0 0.0 0.0 19 0.07973881775918877 0.0 0.0 0.0 0.0 20 0.08876585373192712 0.0 0.0 0.0 0.0 21 0.09779288970466547 0.0 0.0 0.0 0.0 22 0.10230640769103465 0.0 0.0 0.0 0.0 23 0.11283794965922939 0.0 0.0 0.0 0.0 24 0.11584696165014217 0.0 0.0 0.0 0.0 25 0.12036047963651135 0.0 0.0 0.0 0.0 26 0.12487399762288053 0.0 0.0 0.0 0.0 27 0.13991905757744444 0.0 0.0 0.0 0.0 28 0.14744158755472642 0.0 0.0 0.0 0.0 29 0.15496411753200837 0.0 0.0 0.0 0.0 30 0.15797312952292114 0.0 0.0 0.0 0.0 31 0.17753170746385424 0.0 0.0 0.0 0.0 32 0.17903621345931064 0.0 0.0 0.0 0.0 33 0.18054071945476702 0.0 0.0 0.0 0.0 34 0.18204522545022342 0.0 0.0 0.0 0.0 35 0.18354973144567982 0.0 0.0 0.0 0.0 36 0.1850542374411362 0.0 0.0 0.0 0.0 37 0.1865587434365926 0.0 0.0 0.0 0.0 38 0.18956775542750537 0.0 0.0 0.0 0.0 39 0.19257676741841817 0.0 0.0 0.0 0.0 40 0.19859479140024372 0.0 0.0 0.0 0.0 41 0.20160380339115652 0.0 0.0 0.0 0.0 42 0.2046128153820693 0.0 0.0 0.0 0.0 43 0.222666887327546 0.0 0.0 0.0 0.0 44 0.24372997126393547 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTGAA 60 0.0 50.385376 41 GACAAAT 15 0.0081449 50.385376 39 CGTCTCA 20 4.0004496E-4 50.385376 41 GCATTGA 60 0.0 50.385376 40 CGTAGAG 25 1.9741117E-5 50.385372 39 CGACAAA 15 0.008169474 50.34703 38 GCGCATT 65 0.0 50.34703 38 GCGTAGA 25 1.983039E-5 50.34703 38 AGCGTAG 25 2.000994E-5 50.270515 37 GGCGCAT 65 0.0 50.270515 37 TGCTTGC 40 2.5120244E-9 50.232346 35 ATGCTCG 25 2.0100219E-5 50.232346 36 TATGCCT 25 2.0100219E-5 50.232346 35 GCTTGCA 25 2.0100219E-5 50.232346 36 GACGACA 15 0.008243528 50.232346 36 GCGCCTA 15 0.008293173 50.156178 46 ATATGCC 35 5.0622475E-8 50.156178 34 ACCCGCT 15 0.008293173 50.156178 34 AAATTCT 20 4.106951E-4 50.118187 44 AGCCGCA 15 0.00831808 50.11818 44 >>END_MODULE