FastQCFastQC Report
Fri 17 Jun 2016
SRR1526045_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526045_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43189
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAA2140.4954965384704439No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAA1740.4028803630554076No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAA1720.39824955428465586No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAA1460.33804904026488225No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAA1280.29637176132811593No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAA1120.25932529116210146No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAA1100.2546944823913497No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAA1080.25006367362059784No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGAAAAAAAAAAA1020.2361712473083424No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAA1000.23154043853759057No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGAAAAAAAA940.21764801222533517No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAA920.21301720345458333No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAAA910.21070179906920744No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAA880.2037555859130797No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAA780.18060154205932066No Hit
ATGCTTGGGGAGTGCAGTGCTCTGCTTGGGGAAAAAAAAA680.15744749820556161No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGAAAAAAAAAAAA640.14818588066405797No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGGTAAAAAAAAA620.14355507189330616No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAA600.13892426312255435No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGAAAAAA590.13660885873717846No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAA570.13197804996642665No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAA570.13197804996642665No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAA560.12966264558105073No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGTAAAAA550.12734724119567484No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAA540.12503183681029892No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGAAAAAAAA530.12271643242492301No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAA520.12040102803954711No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGGAAAAAAAAAA510.1180856236541712No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAA480.11113941049804349No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGAGTGCAGTGCT470.10882400611266758No Hit
ATGCTTGGGGAGTGCAGTGCTCTGCTTGGGGGTAAAAAAA440.10187779295653986No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGTAAAAAA440.10187779295653986No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGTAAAAA440.10187779295653986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGCA301.4420069E-534.0302714
CAGTGGC301.4420069E-534.0302713
GGGAAGA504.3837645E-1033.872547
CTTGAGG351.0909735E-633.872544
TGGGAAT351.0909735E-633.872546
GGATGGT200.00281077233.8725368
GAAGAGT200.00281077233.8725369
TGGAGAG301.4887748E-533.8725366
GGAAGAC200.00281077233.8725368
TGGGCTC200.00281077233.8725366
TGGGTGT200.00281077233.8725366
GGGAAGT301.4887748E-533.8725367
TTGGAGT408.024108E-833.8725365
CTTGGAG800.031.7555054
TGGGAAG2250.031.614376
GGGGCTA700.031.4530727
TTGGGTG2100.031.453075
TGGGCAG3850.031.233126
GGGAGAG2400.031.0498267
CTGCTAT551.1132215E-931.04502919
Produced by FastQC (version 0.11.5)