FastQCFastQC Report
Fri 17 Jun 2016
SRR1526044_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526044_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3549
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT120.33812341504649196No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAA60.16906170752324598No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAA60.16906170752324598No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAA60.16906170752324598No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAA50.14088475626937164No Hit
ATGCTTGGGGCGTGCAGTGCTATGCTTGGGGGTAAAAAAA50.14088475626937164No Hit
ATGCTTGGGGCGTGCAGTGCTATGCTTGGGGAAAAAAAAA50.14088475626937164No Hit
ATGCTTGGGGAAAAAAACCAAAAAAAAAAAAAAAAAAAAA40.11270780501549732No Hit
ATGCTTGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT40.11270780501549732No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGAAAAAAAAAAAA40.11270780501549732No Hit
ATGCTTGGGAGTGCCGTGCTATGCTTGGGGAAAAAAAAAA40.11270780501549732No Hit
ATGCTTGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT40.11270780501549732No Hit
ATGCTTGGGGCGTGCCGTGCTATGCTTGGGGAAAAAAAAA40.11270780501549732No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGGTAAAAAAAAA40.11270780501549732No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAA40.11270780501549732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGAGT301.0181269E-534.3913046
TGGGAAG200.002290441234.3913046
TGGGGAG850.030.3452686
TGGGGTG401.9038089E-630.0923926
GGGGAGT750.029.8057967
GGGGTGC352.9275237E-529.4782627
CTTGGGC503.098903E-727.5130444
TGGGGAA250.00683472727.5130446
TTGGGGA1200.027.226455
TTGGGGT454.7743324E-626.748795
TTGGGAG454.7743324E-626.748795
TTGGGAC407.286434E-525.793485
GGTGCAG408.072227E-525.4259
CTTGGGA1300.025.1321074
ATGCTTG4800.024.718751
TTGGGGC350.001103587324.5652185
GGGTGCA350.00120400724.2142858
TGCTTGG4950.023.2749232
GCTTGGG4900.023.1614913
GGAGTGC1001.8189894E-1122.0350029
Produced by FastQC (version 0.11.5)