##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526044_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3549 Sequences flagged as poor quality 0 Sequence length 40 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 12.0538179768949 4.0 4.0 22.0 4.0 29.0 2 11.653423499577345 4.0 4.0 22.0 4.0 27.0 3 10.07410538179769 4.0 4.0 17.0 4.0 24.0 4 7.9061707523245985 4.0 4.0 11.0 4.0 15.0 5 10.810650887573965 4.0 4.0 20.0 4.0 26.0 6 11.549168779938011 4.0 4.0 22.0 4.0 29.0 7 11.533953226260918 4.0 4.0 22.0 4.0 27.0 8 12.122287968441814 4.0 4.0 26.0 4.0 29.0 9 10.957171034094111 4.0 4.0 23.0 4.0 27.0 10 8.853198083967314 4.0 4.0 14.0 4.0 22.0 11 10.41730064806988 4.0 4.0 16.0 4.0 30.0 12 10.327979712595097 4.0 4.0 15.0 4.0 30.0 13 10.161735700197239 4.0 4.0 15.0 4.0 30.0 14 10.083403775711467 4.0 4.0 12.0 4.0 31.0 15 9.523809523809524 4.0 4.0 9.0 4.0 30.0 16 7.56720202874049 4.0 4.0 4.0 4.0 22.0 17 7.005353620738236 4.0 4.0 4.0 4.0 19.0 18 6.819385742462665 4.0 4.0 4.0 4.0 17.0 19 6.9442096365173285 4.0 4.0 4.0 4.0 18.0 20 7.001408847562693 4.0 4.0 4.0 4.0 19.0 21 7.251901944209637 4.0 4.0 4.0 4.0 21.0 22 6.916596224288532 4.0 4.0 4.0 4.0 19.0 23 7.158918005071851 4.0 4.0 4.0 4.0 20.0 24 6.860524091293322 4.0 4.0 4.0 4.0 18.0 25 6.9723865877712035 4.0 4.0 4.0 4.0 19.0 26 7.554804170188786 4.0 4.0 4.0 4.0 22.0 27 7.740772048464356 4.0 4.0 4.0 4.0 24.0 28 7.53592561284869 4.0 4.0 4.0 4.0 22.0 29 7.422936038320653 4.0 4.0 4.0 4.0 22.0 30 7.507185122569738 4.0 4.0 4.0 4.0 22.0 31 8.081149619611159 4.0 4.0 4.0 4.0 31.0 32 7.963933502395041 4.0 4.0 4.0 4.0 29.0 33 7.9061707523245985 4.0 4.0 4.0 4.0 29.0 34 7.968441814595661 4.0 4.0 4.0 4.0 30.0 35 7.762468300929839 4.0 4.0 4.0 4.0 29.0 36 7.430825584671738 4.0 4.0 4.0 4.0 28.0 37 7.2735981966751195 4.0 4.0 4.0 4.0 26.0 38 7.236968160045083 4.0 4.0 4.0 4.0 26.0 39 7.217807833192449 4.0 4.0 4.0 4.0 25.0 40 7.2234432234432235 4.0 4.0 4.0 4.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -7.575595631725285 1 2 -8.088697816233118 1 3 -6.30468192839823 1 4 -5.0888193089893985 1 5 -7.368290020135333 1 6 -7.517563078057302 1 7 -7.426475811665155 1 8 -8.059055019292503 1 9 -7.318167670654832 1 10 -5.055039761265693 1 11 -6.3650753824694775 1 12 -6.257676920497845 1 13 -5.459790019209148 1 14 -5.624123987907685 1 15 -4.464218102536458 1 16 -2.5679379990997457 1 17 -2.3048392414887937 1 18 -2.217089888431353 1 19 -2.499976266423637 1 20 -2.152385004454966 1 21 -2.5211400174493894 1 22 -2.499956885932497 1 23 -2.961507380794929 1 24 -2.5590400845054253 1 25 -2.6043026079611433 1 26 -3.1306209676074905 1 27 -3.6126493247160765 1 28 -3.239627593558917 1 29 -3.486542946427414 1 30 -3.004630802801535 1 31 -3.4767560531039363 1 32 -3.532569575268548 1 33 -3.3773081076675435 1 34 -3.560397575609386 1 35 -3.584804143581424 1 36 -2.6385894521966433 1 37 -2.5509478379985433 1 38 -2.4694864470859335 1 39 -2.432485523676154 1 40 -2.43843179178545 7 1 -1.5755956317252853 7 2 -4.088697816233118 7 3 -2.971348595064897 7 4 -2.7554859756560655 7 5 -3.0349566868019995 7 6 -1.517563078057302 7 7 -2.7598091449984885 7 8 -3.3923883526258365 7 9 -0.6515010039881659 7 10 -3.388373094599026 7 11 3.9682579508638565 7 12 4.075656412835489 7 13 4.873543314124186 7 14 -5.624123987907685 7 15 -4.464218102536458 7 16 -2.5679379990997457 7 17 -2.3048392414887937 7 18 -2.217089888431353 7 19 -2.499976266423637 7 20 -2.152385004454966 7 21 -2.5211400174493894 7 22 -2.499956885932497 7 23 -2.961507380794929 7 24 -2.5590400845054253 7 25 -2.6043026079611433 7 26 -3.1306209676074905 7 27 -3.6126493247160765 7 28 -3.239627593558917 7 29 -3.486542946427414 7 30 -3.004630802801535 7 31 -3.4767560531039363 7 32 -3.532569575268548 7 33 -3.3773081076675435 7 34 -3.560397575609386 7 35 -3.584804143581424 7 36 -2.6385894521966433 7 37 -2.5509478379985433 7 38 -2.4694864470859335 7 39 -2.432485523676154 7 40 -2.43843179178545 10 1 2.9244043682747147 10 2 8.911302183766882 10 3 5.19531807160177 10 4 10.411180691010602 10 5 9.631709979864667 10 6 8.482436921942698 10 7 5.573524188334845 10 8 7.440944980707497 10 9 5.681832329345168 10 10 10.944960238734307 10 11 10.634924617530523 10 12 7.742323079502155 10 13 10.040209980790852 10 14 7.375876012092315 10 15 11.035781897463542 10 16 13.432062000900254 10 17 13.695160758511207 10 18 13.282910111568647 10 19 12.000023733576363 10 20 13.847614995545033 10 21 9.97885998255061 10 22 14.500043114067502 10 23 12.538492619205071 10 24 14.440959915494574 10 25 14.395697392038857 10 26 12.369379032392509 10 27 13.387350675283923 10 28 8.260372406441082 10 29 13.513457053572587 10 30 12.995369197198464 10 31 13.523243946896063 10 32 9.467430424731452 10 33 10.622691892332456 10 34 9.439602424390614 10 35 13.415195856418576 10 36 12.861410547803356 10 37 13.449052162001458 10 38 9.030513552914066 10 39 13.567514476323847 10 40 11.561568208214549 11 1 -7.575595631725285 11 2 -8.088697816233118 11 3 -6.30468192839823 11 4 -5.0888193089893985 11 5 -7.368290020135333 11 6 -7.517563078057302 11 7 -7.426475811665155 11 8 -8.059055019292503 11 9 -7.318167670654832 11 10 -5.055039761265693 11 11 -6.3650753824694775 11 12 -6.257676920497845 11 13 -5.459790019209148 11 14 -5.624123987907685 11 15 -4.464218102536458 11 16 -2.5679379990997457 11 17 -2.3048392414887937 11 18 -2.217089888431353 11 19 -2.499976266423637 11 20 -2.152385004454966 11 21 -2.5211400174493894 11 22 -2.499956885932497 11 23 -2.961507380794929 11 24 -2.5590400845054253 11 25 -2.6043026079611433 11 26 -3.1306209676074905 11 27 -3.6126493247160765 11 28 -3.239627593558917 11 29 -3.486542946427414 11 30 -3.004630802801535 11 31 -3.4767560531039363 11 32 -3.532569575268548 11 33 -3.3773081076675435 11 34 -3.560397575609386 11 35 -3.584804143581424 11 36 -2.6385894521966433 11 37 -2.5509478379985433 11 38 -2.4694864470859335 11 39 -2.432485523676154 11 40 -2.43843179178545 12 1 1.9244043682747147 12 2 2.9113021837668818 12 3 5.69531807160177 12 4 0.4111806910106015 12 5 3.6317099798646666 12 6 -1.017563078057302 12 7 3.0735241883348454 12 8 2.4409449807074974 12 9 6.681832329345168 12 10 6.944960238734307 12 11 10.634924617530523 12 12 9.242323079502155 12 13 1.040209980790852 12 14 11.375876012092315 12 15 10.535781897463542 12 16 -2.5679379990997457 12 17 -2.3048392414887937 12 18 -2.217089888431353 12 19 -2.499976266423637 12 20 -2.152385004454966 12 21 -2.5211400174493894 12 22 -2.499956885932497 12 23 -2.961507380794929 12 24 -2.5590400845054253 12 25 -2.6043026079611433 12 26 -3.1306209676074905 12 27 -3.6126493247160765 12 28 -3.239627593558917 12 29 -3.486542946427414 12 30 -3.004630802801535 12 31 -3.4767560531039363 12 32 -3.532569575268548 12 33 -3.3773081076675435 12 34 -3.560397575609386 12 35 -3.584804143581424 12 36 -2.6385894521966433 12 37 -2.5509478379985433 12 38 -2.4694864470859335 12 39 -2.432485523676154 12 40 -2.43843179178545 13 1 17.424404368274715 13 2 8.911302183766882 13 3 5.69531807160177 13 4 1.9111806910106015 13 5 7.631709979864667 13 6 9.482436921942698 13 7 10.573524188334845 13 8 8.940944980707497 13 9 -0.318167670654832 13 10 10.944960238734307 13 11 12.634924617530523 13 12 20.742323079502157 13 13 16.540209980790852 13 14 21.375876012092313 13 15 1.5357818974635418 13 16 10.432062000900254 13 17 15.695160758511207 13 18 20.782910111568647 13 19 10.500023733576363 13 20 5.847614995545034 13 21 3.4788599825506106 13 22 8.500043114067502 13 23 13.038492619205071 13 24 8.440959915494574 13 25 7.395697392038857 13 26 10.869379032392509 13 27 22.387350675283923 13 28 21.760372406441082 13 29 22.513457053572587 13 30 21.995369197198464 13 31 25.523243946896063 13 32 24.467430424731454 13 33 21.622691892332455 13 34 23.439602424390614 13 35 24.415195856418578 13 36 -2.6385894521966433 13 37 -2.5509478379985433 13 38 -2.4694864470859335 13 39 -2.432485523676154 13 40 -2.43843179178545 15 1 7.424404368274715 15 2 9.911302183766882 15 3 6.69531807160177 15 4 1.9111806910106015 15 5 6.631709979864667 15 6 13.482436921942698 15 7 2.5735241883348454 15 8 14.940944980707497 15 9 15.681832329345168 15 10 5.944960238734307 15 11 22.634924617530523 15 12 24.742323079502157 15 13 25.540209980790852 15 14 25.375876012092313 15 15 -4.464218102536458 15 16 -2.5679379990997457 15 17 -2.3048392414887937 15 18 -2.217089888431353 15 19 -2.499976266423637 15 20 -2.152385004454966 15 21 -2.5211400174493894 15 22 -2.499956885932497 15 23 -2.961507380794929 15 24 -2.5590400845054253 15 25 -2.6043026079611433 15 26 -3.1306209676074905 15 27 -3.6126493247160765 15 28 -3.239627593558917 15 29 -3.486542946427414 15 30 -3.004630802801535 15 31 -3.4767560531039363 15 32 -3.532569575268548 15 33 -3.3773081076675435 15 34 -3.560397575609386 15 35 -3.584804143581424 15 36 -2.6385894521966433 15 37 -2.5509478379985433 15 38 -2.4694864470859335 15 39 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1.1389430477524174 16 40 -0.7241460774997357 17 1 6.567261511131857 17 2 5.482730755195455 17 3 5.8381752144589125 17 4 1.3397521195820303 17 5 8.060281408436094 17 6 9.196722636228413 17 7 8.573524188334845 17 8 8.798087837850355 17 9 4.681832329345168 17 10 3.944960238734307 17 11 3.349210331816236 17 12 2.0280373652164414 17 13 3.968781409362281 17 14 5.518733154949457 17 15 4.535781897463542 17 16 3.5749191437573966 17 17 3.552303615654064 17 18 5.640052968711505 17 19 3.7857380192906493 17 20 4.1333292812593205 17 21 6.336002839693468 17 22 5.35718597121036 17 23 7.7527783334907845 17 24 6.298102772637432 17 25 5.538554534895999 17 26 7.8693790323925095 17 27 5.815922103855352 17 28 4.760372406441083 17 29 6.370599910715444 17 30 5.138226340055607 17 31 7.80895823261035 17 32 8.038858996160023 17 33 9.765549035189599 17 34 10.15388813867633 17 35 7.843767284990005 17 36 8.789981976374786 17 37 8.449052162001458 17 38 8.101942124342639 17 39 7.567514476323846 17 40 6.847282493928836 18 1 7.757737701608047 18 2 5.911302183766882 18 3 4.361984738268436 18 4 0.24451402434393543 18 5 8.965043313197999 18 6 8.482436921942698 18 7 8.573524188334845 18 8 9.607611647374165 18 9 -7.318167670654832 18 10 -5.055039761265693 18 11 -6.3650753824694775 18 12 -6.257676920497845 18 13 -5.459790019209148 18 14 -5.624123987907685 18 15 -4.464218102536458 18 16 -2.5679379990997457 18 17 -2.3048392414887937 18 18 -2.217089888431353 18 19 -2.499976266423637 18 20 -2.152385004454966 18 21 -2.5211400174493894 18 22 -2.499956885932497 18 23 -2.961507380794929 18 24 -2.5590400845054253 18 25 -2.6043026079611433 18 26 -3.1306209676074905 18 27 -3.6126493247160765 18 28 -3.239627593558917 18 29 -3.486542946427414 18 30 -3.004630802801535 18 31 -3.4767560531039363 18 32 -3.532569575268548 18 33 -3.3773081076675435 18 34 -3.560397575609386 18 35 -3.584804143581424 18 36 -2.6385894521966433 18 37 -2.5509478379985433 18 38 -2.4694864470859335 18 39 -2.432485523676154 18 40 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23 2.6384926192050706 110 24 3.0409599154945743 110 25 2.9956973920388563 110 26 3.0693790323925088 110 27 2.587350675283923 110 28 0.3603724064410825 110 29 3.313457053572587 110 30 3.195369197198464 110 31 2.1232439468960633 110 32 3.2674304247314527 110 33 2.8226918923324558 110 34 3.2396024243906147 110 35 2.615195856418575 110 36 2.561410547803356 110 37 -0.7509478379985435 110 38 2.930513552914067 110 39 2.9675144763238466 110 40 2.9615682082145502 111 1 0.7101186539890012 111 2 4.911302183766882 111 3 1.4096037858874837 111 4 -1.374533594703684 111 5 3.917424265578953 111 6 4.911008350514125 111 7 1.287809902620559 111 8 1.798087837850355 111 9 2.110403757916597 111 10 -0.7693254755514065 111 11 0.34921033181623606 111 12 2.1708945080735838 111 13 0.25449569507656555 111 14 3.518733154949457 111 15 4.250067611749255 111 16 4.003490572328825 111 17 2.9808750442254928 111 18 4.782910111568647 111 19 1.9285951621477917 111 20 3.847614995545034 111 21 -0.806854303163675 111 22 0.07147168549607397 111 23 -0.10436452365207227 111 24 -0.7018972273625685 111 25 -1.3185883222468577 111 26 0.2979504609639383 111 27 0.10163638956963794 111 28 0.18894383501251166 111 29 -1.057971517855985 111 30 -0.29034508851582075 111 31 0.666101089753206 111 32 0.3245732818743088 111 33 1.0512633209038853 111 34 0.8681738529620429 111 35 -1.1562327150099954 111 36 -1.0671608807680721 111 37 -0.2652335522842577 111 38 0.5305135529140665 111 39 -0.28962838081901054 111 40 -0.009860363214021284 112 1 14.424404368274715 112 2 4.911302183766882 112 3 -0.30468192839822983 112 4 5.9111806910106015 112 5 10.631709979864667 112 6 2.482436921942698 112 7 17.573524188334844 112 8 14.940944980707497 112 9 15.681832329345168 112 10 -0.05503976126569299 112 11 12.634924617530523 112 12 11.742323079502155 112 13 3.540209980790852 112 14 17.375876012092313 112 15 1.5357818974635418 112 16 15.432062000900254 112 17 13.695160758511207 112 18 19.782910111568647 112 19 22.500023733576363 112 20 12.847614995545033 112 21 18.47885998255061 112 22 24.500043114067502 112 23 26.038492619205073 112 24 18.440959915494574 112 25 25.395697392038855 112 26 28.86937903239251 112 27 28.387350675283923 112 28 28.760372406441082 112 29 28.513457053572587 112 30 -3.004630802801535 112 31 -3.4767560531039363 112 32 -3.532569575268548 112 33 -3.3773081076675435 112 34 -3.560397575609386 112 35 -3.584804143581424 112 36 -2.6385894521966433 112 37 -2.5509478379985433 112 38 -2.4694864470859335 112 39 -2.432485523676154 112 40 -2.43843179178545 116 1 26.424404368274715 116 2 25.91130218376688 116 3 24.69531807160177 116 4 28.9111806910106 116 5 26.631709979864667 116 6 19.4824369219427 116 7 9.573524188334845 116 8 -1.0590550192925026 116 9 7.681832329345168 116 10 21.944960238734307 116 11 -6.3650753824694775 116 12 -6.257676920497845 116 13 -5.459790019209148 116 14 -5.624123987907685 116 15 -4.464218102536458 116 16 -2.5679379990997457 116 17 -2.3048392414887937 116 18 -2.217089888431353 116 19 -2.499976266423637 116 20 -2.152385004454966 116 21 -2.5211400174493894 116 22 -2.499956885932497 116 23 -2.961507380794929 116 24 -2.5590400845054253 116 25 -2.6043026079611433 116 26 -3.1306209676074905 116 27 -3.6126493247160765 116 28 -3.239627593558917 116 29 -3.486542946427414 116 30 -3.004630802801535 116 31 -3.4767560531039363 116 32 -3.532569575268548 116 33 -3.3773081076675435 116 34 -3.560397575609386 116 35 -3.584804143581424 116 36 -2.6385894521966433 116 37 -2.5509478379985433 116 38 -2.4694864470859335 116 39 -2.432485523676154 116 40 -2.43843179178545 119 1 -0.5755956317252853 119 2 20.91130218376688 119 3 -5.30468192839823 119 4 12.911180691010602 119 5 21.631709979864667 119 6 21.4824369219427 119 7 21.573524188334844 119 8 15.940944980707497 119 9 21.68183232934517 119 10 -5.055039761265693 119 11 -6.3650753824694775 119 12 -6.257676920497845 119 13 -5.459790019209148 119 14 -5.624123987907685 119 15 -4.464218102536458 119 16 -2.5679379990997457 119 17 -2.3048392414887937 119 18 -2.217089888431353 119 19 -2.499976266423637 119 20 -2.152385004454966 119 21 -2.5211400174493894 119 22 -2.499956885932497 119 23 -2.961507380794929 119 24 -2.5590400845054253 119 25 -2.6043026079611433 119 26 -3.1306209676074905 119 27 -3.6126493247160765 119 28 -3.239627593558917 119 29 -3.486542946427414 119 30 -3.004630802801535 119 31 -3.4767560531039363 119 32 -3.532569575268548 119 33 -3.3773081076675435 119 34 -3.560397575609386 119 35 -3.584804143581424 119 36 -2.6385894521966433 119 37 -2.5509478379985433 119 38 -2.4694864470859335 119 39 -2.432485523676154 119 40 -2.43843179178545 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 2170.0 5 33.0 6 32.0 7 76.0 8 74.0 9 78.0 10 130.0 11 143.0 12 105.0 13 30.0 14 32.0 15 29.0 16 21.0 17 27.0 18 33.0 19 49.0 20 75.0 21 105.0 22 85.0 23 84.0 24 45.0 25 42.0 26 13.0 27 16.0 28 12.0 29 7.0 30 1.0 31 1.0 32 0.0 33 0.0 34 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.11931818181819 0.2556818181818182 0.2840909090909091 0.3409090909090909 8 98.14032121724429 0.9861932938856016 0.4508312200619893 0.422654268808115 9 95.88152327221438 1.7489421720733427 0.9026798307475317 1.466854724964739 10 47.81628627782474 28.120597351366584 7.269653423499578 16.793462947309102 11 31.25704622322435 29.650507328072152 17.925591882750847 21.16685456595265 12 50.01408847562694 12.200619892927584 22.682445759368836 15.102845872076642 13 27.331642716258102 20.23105100028177 31.248238940546635 21.189067342913496 14 34.715172024816695 21.23519458544839 18.527918781725887 25.52171460800902 15 23.41504649196957 21.301775147928996 18.258664412510566 37.02451394759087 16 28.62778247393632 26.232741617357004 23.330515638207945 21.80896027049873 17 36.799098337559876 17.10340941110172 25.331079177233022 20.766413074105383 18 23.837700760777683 16.229923922231613 32.74161735700197 27.19075795998873 19 26.602654617339734 10.98559728890144 24.230443377576954 38.18130471618187 20 14.285714285714285 20.42828965905889 31.924485770639617 33.36151028458721 21 16.483516483516482 25.359256128486894 39.785855170470555 18.371372217526062 22 17.18794026486334 27.810650887573964 29.33220625528318 25.669202592279518 23 23.978585517047055 16.9061707523246 36.63003663003663 22.485207100591715 24 28.430543815159197 21.66807551422936 25.725556494787266 24.175824175824175 25 16.35131318836487 14.402711098559728 35.554927986444504 33.6910477266309 26 21.341979137299123 14.491119255709052 43.27600789399493 20.8908937129969 27 30.20039514535704 15.523567598080723 35.90177815410669 18.374259102455547 28 44.315937940761636 15.373765867418902 23.1311706629055 17.179125528913964 29 44.68265162200282 17.376586741889984 21.7771509167842 16.16361071932299 30 44.604113834883066 19.38574246266554 18.512256973795434 17.49788672865596 31 35.108481262327416 24.28853198083967 20.174697097774022 20.42828965905889 32 26.458157227387996 29.980276134122285 19.949281487743026 23.61228515074669 33 18.212574006202427 32.11164364251481 21.341979137299123 28.33380321398365 34 13.52493660185968 39.81403212172443 21.442659904198365 25.218371372217526 35 10.622710622710622 38.9687235841082 20.794590025359255 29.61397576782192 36 10.033821871476889 41.88275084554679 21.25140924464487 26.832018038331455 37 9.73401245048104 43.01075268817204 21.137521222410864 26.117713638936053 38 8.086785009861932 40.40574809805579 20.597351366582135 30.910115525500142 39 8.875739644970414 40.60298675683291 21.611721611721613 28.909551986475062 40 9.326570865032403 42.80078895463511 21.639898562975485 26.232741617357004 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 2.0 12 4.0 13 4.0 14 6.0 15 8.0 16 13.5 17 19.0 18 19.0 19 29.5 20 40.0 21 45.0 22 50.0 23 50.0 24 56.0 25 62.0 26 62.0 27 62.0 28 62.0 29 57.5 30 53.0 31 52.0 32 51.0 33 51.0 34 62.0 35 73.0 36 82.0 37 91.0 38 91.0 39 111.0 40 131.0 41 147.0 42 163.0 43 163.0 44 202.5 45 242.0 46 266.0 47 290.0 48 290.0 49 291.0 50 292.0 51 304.5 52 317.0 53 317.0 54 323.0 55 329.0 56 322.5 57 316.0 58 316.0 59 318.0 60 320.0 61 277.0 62 234.0 63 234.0 64 196.5 65 159.0 66 133.0 67 107.0 68 107.0 69 85.0 70 63.0 71 48.0 72 33.0 73 33.0 74 25.5 75 18.0 76 14.0 77 10.0 78 10.0 79 8.5 80 7.0 81 4.5 82 2.0 83 2.0 84 1.5 85 1.0 86 1.0 87 1.0 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.8171315863623556 8 0.0 9 0.11270780501549732 10 0.0 11 0.02817695125387433 12 0.0 13 0.0 14 0.08453085376162299 15 0.0 16 0.0 17 0.0 18 0.0 19 0.22541561003099464 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.22541561003099464 26 0.05635390250774866 27 0.16906170752324598 28 0.11270780501549732 29 0.11270780501549732 30 0.0 31 0.0 32 0.0 33 0.05635390250774866 34 0.0 35 0.0 36 0.02817695125387433 37 0.422654268808115 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 3549.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.57621865314174 #Duplication Level Percentage of deduplicated Percentage of total 1 97.25825471698113 92.95576218653142 2 1.7983490566037736 3.4375880529726683 3 0.5011792452830188 1.4370245139475908 4 0.2358490566037736 0.9016624401239786 5 0.0884433962264151 0.422654268808115 6 0.0884433962264151 0.5071851225697379 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0294811320754717 0.33812341504649196 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGCTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.33812341504649196 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAA 6 0.16906170752324598 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAA 6 0.16906170752324598 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAA 6 0.16906170752324598 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAA 5 0.14088475626937164 No Hit ATGCTTGGGGCGTGCAGTGCTATGCTTGGGGGTAAAAAAA 5 0.14088475626937164 No Hit ATGCTTGGGGCGTGCAGTGCTATGCTTGGGGAAAAAAAAA 5 0.14088475626937164 No Hit ATGCTTGGGGAAAAAAACCAAAAAAAAAAAAAAAAAAAAA 4 0.11270780501549732 No Hit ATGCTTGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.11270780501549732 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGAAAAAAAAAAAA 4 0.11270780501549732 No Hit ATGCTTGGGAGTGCCGTGCTATGCTTGGGGAAAAAAAAAA 4 0.11270780501549732 No Hit ATGCTTGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT 4 0.11270780501549732 No Hit ATGCTTGGGGCGTGCCGTGCTATGCTTGGGGAAAAAAAAA 4 0.11270780501549732 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGGGTAAAAAAAAA 4 0.11270780501549732 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAA 4 0.11270780501549732 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.02817695125387433 0.0 0.0 0.0 0.0 25 0.02817695125387433 0.0 0.0 0.0 0.0 26 0.02817695125387433 0.0 0.0 0.0 0.0 27 0.02817695125387433 0.0 0.0 0.0 0.0 28 0.02817695125387433 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGAGT 30 1.0181269E-5 34.391304 6 TGGGAAG 20 0.0022904412 34.391304 6 TGGGGAG 85 0.0 30.345268 6 TGGGGTG 40 1.9038089E-6 30.092392 6 GGGGAGT 75 0.0 29.805796 7 GGGGTGC 35 2.9275237E-5 29.478262 7 CTTGGGC 50 3.098903E-7 27.513044 4 TGGGGAA 25 0.006834727 27.513044 6 TTGGGGA 120 0.0 27.22645 5 TTGGGGT 45 4.7743324E-6 26.74879 5 TTGGGAG 45 4.7743324E-6 26.74879 5 TTGGGAC 40 7.286434E-5 25.79348 5 GGTGCAG 40 8.072227E-5 25.425 9 CTTGGGA 130 0.0 25.132107 4 ATGCTTG 480 0.0 24.71875 1 TTGGGGC 35 0.0011035873 24.565218 5 GGGTGCA 35 0.001204007 24.214285 8 TGCTTGG 495 0.0 23.274923 2 GCTTGGG 490 0.0 23.161491 3 GGAGTGC 100 1.8189894E-11 22.035002 9 >>END_MODULE