FastQCFastQC Report
Fri 17 Jun 2016
SRR1526043_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526043_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6104
Sequences flagged as poor quality0
Sequence length40
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG811.3269986893840104No Hit
ATGCTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT781.2778505897771952No Hit
ATGCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT590.9665792922673657No Hit
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC480.7863695937090431No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.49148099606815204No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.27850589777195284No Hit
ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA170.27850589777195284No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG170.27850589777195284No Hit
ATGCTTGGGGGGGAGGTAGTGACGAAAAATAACAATACAG150.24574049803407602No Hit
ATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.22935779816513763No Hit
ATGCTTGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT140.22935779816513763No Hit
ATGCTTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC130.21297509829619923No Hit
ATGCTTGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA120.19659239842726078No Hit
ATGCTTGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG120.19659239842726078No Hit
ATGCTTGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT120.19659239842726078No Hit
ATGCTTGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG120.19659239842726078No Hit
ATGCTTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT120.19659239842726078No Hit
ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGA110.18020969855832242No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG100.163826998689384No Hit
ATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.163826998689384No Hit
ATGCTTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT100.163826998689384No Hit
ATGCTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCG100.163826998689384No Hit
ATGCTTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC100.163826998689384No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAA100.163826998689384No Hit
ATGCTTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC90.1474442988204456No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAA90.1474442988204456No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAA90.1474442988204456No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAA80.1310615989515072No Hit
ATGCTTGGGAAGAAAATGTTTGAAACAACAAGATTGCTTG80.1310615989515072No Hit
ATGCTTGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGT80.1310615989515072No Hit
ATGCTTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAG70.11467889908256881No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAA70.11467889908256881No Hit
ATGCTTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTG70.11467889908256881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTTTT301.2003624E-534.2231431
GATGAGT301.2003624E-534.2231428
AGATGAG301.2003624E-534.2231427
AGTTTTT301.2003624E-534.2231432
TGAGTTT301.2003624E-534.2231430
ATGAGTT301.2003624E-534.2231429
GAGATGA301.2003624E-534.2231426
ATCCTGC200.00259958833.94262320
CTGAGAT301.2718172E-533.94262324
GTACCTG301.2718172E-533.9426239
CCTGCCA200.00259958833.94262322
GGGTACC301.2718172E-533.9426237
TGATCCT251.8201908E-433.94262318
TTTTTGT301.2718172E-533.94262334
GTTTTTG301.2718172E-533.94262333
TTCTGAG301.2718172E-533.94262322
TTGGGTG200.00259958833.9426235
TTGGGTA406.1547325E-833.9426235
TTGGGGC454.256435E-933.9426235
TGGGGGC358.8627894E-733.9426236
Produced by FastQC (version 0.11.5)