FastQCFastQC Report
Fri 17 Jun 2016
SRR1526042_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526042_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44775
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG26205.8514796203238415No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG6371.4226689000558348No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG3140.701284198771636No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG2770.6186487995533222No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG1890.4221105527638191No Hit
GTCGGTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT1520.33947515354550534No Hit
GTCGGTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC1190.2657733109994417No Hit
GTCGGTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC890.1987716359575656No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC860.192071468453378No Hit
GTCGGTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC760.16973757677275267No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC720.16080402010050251No Hit
GTCGGTGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT710.15857063093243998No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC700.15633724176437744No Hit
GTCGGTGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT690.1541038525963149No Hit
GTCGGTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA670.14963707426018982No Hit
GTCGGTGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT670.14963707426018982No Hit
GTCGGTGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG620.13847012841987716No Hit
GTCGGTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC610.13623673925181462No Hit
GTCGGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT590.13176996091568957No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTACG570.1273031825795645No Hit
GTCGGTGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC560.12506979341150196No Hit
GTCGGTGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG530.11836962590731435No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG510.11390284757118929No Hit
GTCGGTGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG500.11166945840312675No Hit
GTCGGTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC470.10496929089893914No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTAGTCTCAAAGATTAAG470.10496929089893914No Hit
GTCGGTGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG450.10050251256281408No Hit
GTCGGTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA450.10050251256281408No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCC150.0079413550.6807339
TCGTCTC800.050.6807340
GTAGCCC150.0079413550.6807340
CGTCTCA950.050.6807341
TGCTCGT850.050.5654237
GTAGTAG150.00801330150.56541437
ATGCCCG150.00804945750.50795436
TGTAGTA150.00804945750.50795436
TATGCCC150.00808573550.45062335
ATGTAGT150.00808573550.45062335
TCAACGA251.9881329E-550.27941545
TCCACGA150.00819529950.2794145
TTAAAGA150.00819529950.2794145
TAAAGAT150.00823206550.222646
CAGCATT150.00826895250.16591643
CAAGATT700.050.16591347
AACGATT252.0149637E-550.16591347
CGCCCAA355.003858E-850.16591347
TTGAACA204.073022E-450.16591343
CTTAAAG252.0284911E-550.1093644
Produced by FastQC (version 0.11.5)