##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526042_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 44775 Sequences flagged as poor quality 0 Sequence length 56 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.2206365159129 38.0 38.0 39.0 34.0 40.0 2 37.19361250697934 38.0 38.0 39.0 34.0 40.0 3 37.28752652149637 38.0 38.0 39.0 34.0 40.0 4 37.182043551088775 38.0 38.0 39.0 34.0 40.0 5 36.7706085985483 38.0 37.0 39.0 33.0 40.0 6 35.47807928531547 38.0 35.0 39.0 29.0 40.0 7 36.86898939140145 38.0 38.0 39.0 33.0 40.0 8 36.55379117811279 38.0 37.0 39.0 32.0 40.0 9 35.38311557788945 38.0 35.0 39.0 29.0 40.0 10 34.9699162479062 38.0 35.0 39.0 28.0 40.0 11 33.58653266331658 37.0 32.0 39.0 23.0 40.0 12 33.18090452261306 37.0 31.0 39.0 21.0 40.0 13 32.856281407035176 37.0 31.0 39.0 19.0 40.0 14 33.48145170295924 38.0 32.0 39.0 21.0 40.0 15 32.47461753210497 36.0 31.0 38.0 17.0 40.0 16 32.676627582356225 37.0 30.0 39.0 19.0 40.0 17 31.72006700167504 36.0 28.0 38.0 16.0 40.0 18 31.081340033500837 35.0 27.0 38.0 14.0 40.0 19 32.04911222780569 36.0 29.0 38.0 18.0 40.0 20 32.61902847571189 36.0 30.0 38.0 20.0 40.0 21 32.79564489112228 37.0 31.0 39.0 19.0 40.0 22 32.161608040201 36.0 29.0 39.0 17.0 40.0 23 32.875734226689 37.0 31.0 39.0 19.0 40.0 24 32.222936906756004 36.0 29.0 39.0 17.0 40.0 25 32.60169737576773 36.0 30.0 39.0 19.0 40.0 26 33.046275823562254 38.0 32.0 39.0 19.0 40.0 27 33.36904522613065 38.0 33.0 39.0 21.0 40.0 28 33.07039642657733 37.0 32.0 39.0 21.0 40.0 29 32.69681742043551 37.0 31.0 39.0 18.0 40.0 30 32.30780569514238 36.0 31.0 38.0 17.0 39.0 31 33.09268565047459 37.0 33.0 39.0 19.0 40.0 32 32.949838079285314 37.0 32.0 39.0 19.0 40.0 33 32.744567280848685 37.0 32.0 38.0 18.0 40.0 34 32.60640982691234 36.0 32.0 38.0 18.0 39.0 35 32.45976549413735 36.0 31.0 38.0 17.0 40.0 36 31.99754327191513 36.0 31.0 38.0 13.0 39.0 37 31.764198771635957 36.0 30.0 38.0 11.0 39.0 38 30.96920156337242 35.0 29.0 38.0 4.0 39.0 39 29.898179787828028 35.0 27.0 38.0 4.0 39.0 40 28.065214963707426 34.0 20.0 38.0 4.0 39.0 41 26.12745951982133 31.0 15.0 37.0 4.0 39.0 42 26.958391959798995 32.0 19.0 37.0 4.0 39.0 43 27.058001116694584 32.0 20.0 37.0 4.0 39.0 44 25.996314907872698 31.0 16.0 37.0 4.0 39.0 45 26.910217755443885 32.0 20.0 37.0 4.0 39.0 46 24.6435734226689 29.0 13.0 36.0 4.0 39.0 47 23.938090452261306 28.0 10.0 35.0 4.0 38.0 48 24.616013400335007 29.0 11.0 36.0 4.0 39.0 49 23.550128419877165 28.0 7.0 35.0 4.0 38.0 50 24.041161362367394 29.0 4.0 35.0 4.0 38.0 51 24.296996091568957 30.0 4.0 36.0 4.0 38.0 52 24.165985482970406 30.0 4.0 36.0 4.0 39.0 53 23.923126744835287 30.0 4.0 36.0 4.0 38.0 54 23.531278615298717 30.0 4.0 35.0 4.0 38.0 55 23.284578447794527 30.0 4.0 35.0 4.0 38.0 56 22.473545505304298 28.0 4.0 35.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.18294400561561 1 2 1.2352201233184985 1 3 1.3729832593147648 1 4 1.6214260318161848 1 5 2.327490841657003 1 6 3.700879255049962 1 7 3.040301276455864 1 8 3.41229087666116 1 9 4.656122477431943 1 10 4.198375455261669 1 11 -2.0547767703462583 1 12 -10.077826859522979 1 13 -19.66932370012688 1 14 -8.663034157435131 1 15 -0.5849828927984397 1 16 -1.0180732486574833 1 17 -4.485133061070417 1 18 -3.9324286500904897 1 19 0.5251504319142306 1 20 -0.3546230856832082 1 21 6.77571006647732 1 22 7.34717782798435 1 23 6.740265884443794 1 24 7.635590694407952 1 25 6.4680715650966185 1 26 6.568381312667519 1 27 6.3195727279585014 1 28 7.142631175563277 1 29 7.144085399235532 1 30 7.469634202414916 1 31 5.657054204225496 1 32 6.27757286607201 1 33 5.738363491475155 1 34 5.729105895203993 1 35 6.735184439063573 1 36 4.697897621079285 1 37 4.454128238788357 1 38 5.582716338067943 1 39 4.30980694628084 1 40 -10.32857922446562 1 41 -0.4270990139313433 1 42 -2.2090774787147254 1 43 -9.877282454872093 1 44 -14.700488846621992 1 45 -2.8595755141107944 1 46 -5.891197794953332 1 47 -9.980110034221475 1 48 -5.85663590843178 1 49 -13.650501950017073 1 50 -4.874863448004074 1 51 1.0847126851852522 1 52 2.087766813889779 1 53 1.5724547531495503 1 54 2.1875882339095085 1 55 1.8930353982170054 1 56 4.995837018209567 2 1 2.189793320684103 2 2 1.4857724874369254 2 3 1.5033411912635017 2 4 1.5615497613787142 2 5 1.7099477572557689 2 6 2.8378655564198283 2 7 2.8197975203798578 2 8 3.38842874674512 2 9 4.324041169433713 2 10 3.611322870197597 2 11 3.3064693631049096 2 12 -0.9735405139639894 2 13 -0.2822269259333332 2 14 3.9540670356713647 2 15 4.342325989216587 2 16 4.7388865392346915 2 17 6.218357880158042 2 18 4.792935910227669 2 19 6.201686004163456 2 20 5.671006609411798 2 21 6.586138701033217 2 22 7.517085030812449 2 23 6.837481969286927 2 24 6.665860248097744 2 25 6.640630115693174 2 26 6.2230432658270445 2 27 5.664689829151605 2 28 5.560660340388733 2 29 6.315981112889968 2 30 5.118551568742795 2 31 4.653960965162305 2 32 5.101700130764893 2 33 4.812822174639102 2 34 4.400780663211059 2 35 3.801026241980054 2 36 4.696571947195061 2 37 4.846527708518806 2 38 5.808964680975588 2 39 5.633934210973724 2 40 3.592764125070392 2 41 7.459113977672722 2 42 5.980935778024115 2 43 4.746668053302894 2 44 -3.9954512858619395 2 45 5.576792139446429 2 46 5.489712501113836 2 47 2.9935974337414066 2 48 5.480748095103351 2 49 -0.30361728364499996 2 50 6.324429525924341 2 51 8.061513392211324 2 52 7.674819398953851 2 53 8.016555504364753 2 54 7.800712405363331 2 55 7.613760099940379 2 56 7.361723010255524 3 1 2.3267796220539694 3 2 1.554265638121862 3 3 1.6814233830443257 3 4 1.6574401723376155 3 5 2.2304957024612477 3 6 3.55019432354311 3 7 3.271852314900407 3 8 3.5391136782519723 3 9 4.762397333817276 3 10 4.611322870197597 3 11 5.457154294611762 3 12 4.7935827737072465 3 13 6.060238827491322 3 14 5.6663958027946535 3 15 -0.09603017516697676 3 16 5.821078320056614 3 17 7.533426373308725 3 18 8.409374266392057 3 19 6.845521620601815 3 20 6.451828527220016 3 21 7.599837331170203 3 22 8.366400099305604 3 23 7.412824435040349 3 24 7.8439424398785675 3 25 7.435150663638378 3 26 6.483317238429784 3 27 6.760580240110514 3 28 6.067509655457226 3 29 7.261186592342025 3 30 6.639099513948274 3 31 6.2840979514636786 3 32 6.320878212956675 3 33 5.908712585598003 3 34 6.058314909786404 3 35 6.19828651595266 3 36 7.628078796510124 3 37 7.435568804409215 3 38 7.302115365907095 3 39 9.401057498644956 3 40 10.250298371645737 3 41 12.637196169453542 3 42 10.501483723229597 3 43 11.185024217686458 3 44 11.52509665934354 3 45 11.49460035862451 3 46 12.48971250111384 3 47 13.760720721412635 3 48 13.11088508140472 3 49 13.189533401286504 3 50 12.502511717705161 3 51 12.034116131937353 3 52 12.085778303063442 3 53 12.605596600255165 3 54 12.184274049198944 3 55 12.846636812269146 3 56 10.882270955461006 4 1 2.4962773389489428 4 2 1.8107039942862428 4 3 1.973843474368529 4 4 1.7816410855796292 4 5 2.7431897663881912 4 6 3.9942125883832986 4 7 3.488473319466614 4 8 3.706054317521378 4 9 5.19655258495883 4 10 4.826117390745537 4 11 6.705008175890299 4 12 6.181527979186701 4 13 6.957773074066669 4 14 6.679546487726164 4 15 6.352006354513389 4 16 7.6356901922027305 4 17 8.880458336779046 4 18 8.604990704748218 4 19 8.252462259871223 4 20 7.3318285272200185 4 21 7.877645550348287 4 22 8.175349870995102 4 23 8.19876050809971 4 24 8.05344015677354 4 25 7.6932328554192 4 26 6.603865183635264 4 27 5.796561975270329 4 28 6.0901580572837055 4 29 6.679999377730152 4 30 5.936085815318137 4 31 6.138344526806144 4 32 5.979508349942979 4 33 6.4349226312601076 4 34 6.2108263253115155 4 35 6.444861858418413 4 36 6.972736330756703 4 37 7.995203507605559 4 38 7.703576553121703 4 39 7.671742430151806 4 40 3.111850883061262 4 41 12.083223566713816 4 42 11.424040800855167 4 43 11.298631523622529 4 44 11.424457389937146 4 45 12.15268255040533 4 46 12.951812957734841 4 47 13.950857707714011 4 48 13.676912478664999 4 49 13.620035684391532 4 50 14.394383863823883 4 51 13.014390104540091 4 52 14.540024878405909 4 53 14.008153677880731 4 54 14.16801834143639 4 55 13.939056903593347 4 56 12.620353147241829 5 1 1.4716763999818099 5 2 0.9030983604834262 5 3 1.006331737279325 5 4 0.9206082217298643 5 5 1.1818752123975784 5 6 2.5459496775851704 5 7 2.451478014302296 5 8 2.6162890593054158 5 9 3.7263178431747903 5 10 3.792877954125821 5 11 5.223505828472462 5 12 4.271105443975433 5 13 4.816364623362443 5 14 4.849687332796584 5 15 5.8391425047481285 5 16 6.256347469198033 5 17 7.0541672569668386 5 18 7.794286479396106 5 19 6.1057955932045545 5 20 5.691265146938328 5 21 5.785251184151104 5 22 6.497791185549087 5 23 5.0614835127945454 5 24 5.65081104879232 5 25 5.021683559644554 5 26 4.34189335264935 5 27 3.356374181843101 5 28 2.1985148647954453 5 29 3.9596237908756926 5 30 3.5366491955998285 5 31 2.423978329623047 5 32 1.9772548288162177 5 33 2.775392114828179 5 34 2.7422817243725532 5 35 2.4730308725029175 5 36 3.8696377392074055 5 37 3.4782082024412446 5 38 4.6104310132155994 5 39 5.340474824518008 5 40 6.255700648319479 5 41 8.8843503272772 5 42 8.498782584892723 5 43 7.881917908599053 5 44 8.251124056603814 5 45 6.883950156039138 5 46 9.536789483556436 5 47 10.1514210879957 5 48 9.991842056129787 5 49 9.805200003640355 5 50 9.475886211241725 5 51 8.942277428483752 5 52 8.78903896291295 5 53 9.504303912622518 5 54 8.849332895427 5 55 9.706756434109781 5 56 8.35612779512915 6 1 1.4746106722822745 6 2 1.0457039942862423 6 3 1.147176807701861 6 4 1.5788633078018464 6 5 1.914856433054858 6 6 2.631434810605519 6 7 3.034028875022166 6 8 3.0771654286324903 6 9 4.293219251625494 6 10 3.9855618351899835 6 11 5.710008175890302 6 12 5.91097242363114 6 13 6.03721751851111 6 14 5.506768709948382 6 15 6.497006354513385 6 16 7.021801303313843 6 17 7.936013892334596 6 18 9.078324038081554 6 19 7.397462259871219 6 20 6.910161860553352 6 21 6.295423328126066 6 22 7.702016537661766 6 23 6.260427174766377 6 24 6.866773490106873 6 25 7.252121744308088 6 26 5.2121985169685985 6 27 4.568228641936997 6 28 4.986269168394816 6 29 5.508332711063485 6 30 6.6371969264292545 6 31 4.860011193472808 6 32 4.991730572165199 6 33 5.099922631260107 6 34 5.180270769755964 6 35 4.542084080640635 6 36 5.608291886312259 6 37 4.810759063161115 6 38 6.106909886455039 6 39 5.954520207929587 6 40 4.12796199417237 6 41 10.224334677824931 6 42 9.389040800855163 6 43 9.528631523622533 6 44 10.2650129454927 6 45 9.63101588373867 6 46 10.41570184662373 6 47 10.390857707714009 6 48 10.809134700887217 6 49 11.305591239947084 6 50 10.14216164160166 6 51 8.738834548984535 6 52 10.56446932285035 6 53 10.632042566769623 6 54 10.574685008103057 6 55 10.659612459148903 6 56 10.449242036130716 7 1 2.2435500662216725 7 2 1.1858555094377579 7 3 1.283540444065494 7 4 1.587701691640234 7 5 2.289856433054858 7 6 3.413000467171173 7 7 3.122412713406007 7 8 3.329690681157743 7 9 4.7856434940497365 7 10 4.075208299836447 7 11 5.91834150922363 7 12 5.826376464035182 7 13 6.2632276195212135 7 14 5.76560709378677 7 15 6.993218475725509 7 16 7.338720495233034 7 17 7.547125003445711 7 18 8.563172522930039 7 19 6.908825896234859 7 20 6.1487982241897114 7 21 6.396433429136163 7 22 6.717168052813282 7 23 5.976336265675471 7 24 6.791015914349302 7 25 6.3859601281464755 7 26 4.882653062423138 7 27 5.200804399512755 7 28 5.295612602738249 7 29 5.5727266504574295 7 30 5.533661572893898 7 31 3.7728899813515966 7 32 3.9285992590338843 7 33 4.24764990398738 7 34 4.368402082887279 7 35 3.8539527675093197 7 36 4.946675724696092 7 37 4.785506537908596 7 38 6.20539473493989 7 39 6.748712127121504 7 40 6.361547852758232 7 41 10.42382962731988 7 42 9.248889285703648 7 43 8.990752735743744 7 44 9.675366480846236 7 45 9.475712853435638 7 46 11.697267503189384 7 47 11.390857707714009 7 48 10.82176096351348 7 49 11.2727629571188 7 50 10.644686894126913 7 51 9.945905256055244 7 52 9.95335821173924 7 53 9.625729435456492 7 54 9.02923046264851 7 55 9.312390236926682 7 56 8.257322844211522 8 1 2.285885182086197 8 2 1.4648216413450683 8 3 1.544235631231274 8 4 1.6826214777364896 8 5 1.9418172173685804 8 6 3.362643960932317 8 7 3.1451399861332803 8 8 3.475858239090009 8 9 4.793219251625494 8 10 4.136705626039657 8 11 6.134027783733437 8 12 6.253292685069052 8 13 6.203067191713725 8 14 5.9095464877261605 8 15 6.925927923140836 8 16 6.633729407889007 8 17 7.66477206226924 8 18 7.6567554106305735 8 19 7.32638382849867 8 20 6.863593233102371 8 21 7.03235143270123 8 22 6.893193008249998 8 23 6.091309527707551 8 24 7.155989176381382 8 25 6.069115208360376 8 26 5.886218124811734 8 27 5.506954132133075 8 28 4.8285894298327285 8 29 5.7607836914556465 8 30 6.143732874141669 8 31 5.17128570327673 8 32 4.769508349942978 8 33 5.568059886162068 8 34 5.380434168448772 8 35 4.988979505477239 8 36 4.6115598601684695 8 37 5.745399586036932 8 38 6.253968709984452 8 39 7.257624783092982 8 40 1.6173410791396918 8 41 10.06910591965499 8 42 9.86208001654144 8 43 9.710004072642139 8 44 10.662888762486169 8 45 9.760917844522979 8 46 12.847891389107392 8 47 12.626151825361067 8 48 13.057500713959111 8 49 12.201016076548392 8 50 12.109481903039573 8 51 12.10321363395186 8 52 12.51708370193532 8 53 12.52501642297877 8 54 11.64821441986776 8 55 11.827096119279624 8 56 11.001529617830062 9 1 2.016277338948946 9 2 0.6404009639832111 9 3 1.0259646864897363 9 4 1.375580479519023 9 5 1.683795826994249 9 6 2.488758042928751 9 7 2.8951399861332803 9 8 3.2084785599456183 9 9 4.300795009201252 9 10 3.908541633169783 9 11 6.160765751647872 9 12 5.7203158579745725 9 13 6.3541367104303 9 14 5.401970730150403 9 15 6.826551809058845 9 16 6.869023525536065 9 17 8.01682197314268 9 18 8.30559676535428 9 19 6.954280441689406 9 20 7.330616406007891 9 21 7.305524338227077 9 22 7.520198355843586 9 23 6.597548386887588 9 24 7.397076520409904 9 25 6.3859601281464755 9 26 6.215986395756474 9 27 5.306865005573357 9 28 5.977430784556436 9 29 6.057575135305914 9 30 5.59426763349996 9 31 5.333496041957659 9 32 4.383144713579345 9 33 5.671892328229802 9 34 5.974462688947881 9 35 4.884255797812351 9 36 6.613342391362764 9 37 6.376415628817682 9 38 5.584182613727766 9 39 6.945681824091199 9 40 2.876699367909744 9 41 9.772314475804723 9 42 9.294343831158194 9 43 9.430146675137681 9 44 10.08445738993715 9 45 8.430258307981092 9 46 10.394237200159086 9 47 10.845403162259462 9 48 9.655094296846812 9 49 11.287914472270316 9 50 10.523474772914795 9 51 9.900450710600698 9 52 9.30184306022409 9 53 10.777244586971644 9 54 9.317109250527299 9 55 9.25178417632062 9 56 8.151262238150917 10 1 1.9248794894865782 10 2 1.0094136717055946 10 3 1.2116929367341172 10 4 1.3633615156871528 10 5 2.0371682610118427 10 6 3.15249215827577 10 7 2.7338496635526326 10 8 3.347774747628904 10 9 4.0916063483996865 10 10 4.1338593262294125 10 11 5.235545810298902 10 12 5.6641086243479855 10 13 6.814547267615051 10 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3.9372472473972344 114 52 4.310501068882097 114 53 5.0110108207378765 114 54 4.812780246198294 114 55 4.098104522640966 114 56 7.441305528194206 115 1 1.796580369251977 115 2 1.2616130851953358 115 3 1.215358625883681 115 4 1.4058835098220541 115 5 2.0247049179033425 115 6 3.3599701641408686 115 7 2.3951399861332803 115 8 2.9130240144910786 115 9 3.9295828879891275 115 10 3.1661173907455407 115 11 0.6380384789205991 115 12 -0.9084720208133028 115 13 -1.7367723804787865 115 14 -4.522271694092023 115 15 -9.158296675789643 115 16 2.5281144346269713 115 17 3.0471250034457107 115 18 4.199536159293672 115 19 3.651250138659101 115 20 3.451828527220016 115 21 5.926736459439194 115 22 6.770198355843586 115 23 5.999063538402744 115 24 7.154652277985662 115 25 5.999596491782835 115 26 5.435683365453443 115 27 4.632622581330935 115 28 3.8486429057685534 115 29 4.625756953487727 115 30 5.253358542590867 115 31 3.29561725407887 115 32 3.474053804488431 115 33 4.255225661563138 115 34 4.413856628341819 115 35 4.35395276750932 115 36 5.257281785302155 115 37 6.444597446999502 115 38 7.038728068273219 115 39 7.506287884697262 115 40 8.853972095182474 115 41 9.840496293986543 115 42 9.120101406915772 115 43 6.46802546301647 115 44 0.2283967838765406 115 45 9.013591641314427 115 46 2.886661442583325 115 47 -7.0182332013768995 115 48 2.768730660483179 115 49 -0.6893582550024107 115 50 3.6977171971572176 115 51 5.044390104540092 115 52 5.279115787496817 115 53 6.22421428394134 115 54 5.370139553557603 115 55 5.448753873290318 115 56 9.196716783605464 116 1 2.071832894504496 116 2 0.9855188091010589 116 3 1.2953249558500062 116 4 1.426085530024075 116 5 1.5861527293511486 116 6 2.4971755513462597 116 7 2.5247696157629065 116 8 2.929017280484338 116 9 4.543219251625494 116 10 3.9809322055603573 116 11 3.4183415092236302 116 12 2.248935386594102 116 13 4.088143444437041 116 14 3.6965835247631986 116 15 -5.183549201042169 116 16 3.2579124144249505 116 17 4.158236114556825 116 18 3.46721292697044 116 19 4.679869667278631 116 20 3.5629396383311303 116 21 5.665793698496433 116 22 6.535349870995102 116 23 5.903945693284896 116 24 6.718625341958727 116 25 5.131751373937721 116 26 4.80016147993156 116 27 4.350636049344402 116 28 3.176083983209633 116 29 3.9851845629153395 116 30 4.609419148651476 116 31 4.781307489769105 116 32 4.260249090683718 116 33 4.845293001630473 116 34 4.499715214200407 116 35 3.5930100065665584 116 36 5.904588182608556 116 37 5.940388692790748 116 38 6.542095071640222 116 39 8.01470539311477 116 40 9.02148051269089 116 41 9.622482825973076 116 42 7.541818578632942 116 43 1.7786315236225292 116 44 3.714087019566776 116 45 7.431941809664593 116 46 1.5221833281052106 116 47 -4.423957107100804 116 48 2.2998754416279574 116 49 0.6111467955026413 116 50 2.7995690490090688 116 51 5.9980938082437945 116 52 5.675580433961464 116 53 6.627412937139994 116 54 6.741351674769724 116 55 6.34942727396372 116 56 8.171464258352938 117 1 1.3329440056156088 117 2 0.5957039942862394 117 3 0.8721768077018623 117 4 0.664974418912962 117 5 1.4315230997215238 117 6 2.325879255049962 117 7 2.095139986133283 117 8 2.774387650854713 117 9 2.7682192516254958 117 10 1.8161173907455392 117 11 -0.7983251574430348 117 12 -5.633472020813304 117 13 -7.7322269259333325 117 14 -2.76545351227384 117 15 -6.54466031215328 117 16 -7.180976474463936 117 17 1.647125003445712 117 18 -2.3800092952517815 117 19 1.3557955932045545 117 20 1.4018285272200153 117 21 3.367645550348289 117 22 4.752016537661763 117 23 3.1354271747663773 117 24 4.42510682344021 117 25 4.688232855419205 117 26 3.353865183635264 117 27 4.209895308603663 117 28 2.691824723950372 117 29 2.6416660443968176 117 30 4.076085815318137 117 31 4.643344526806146 117 32 4.744508349942979 117 33 3.7415892979267724 117 34 4.902492991978185 117 35 3.2948618584184146 117 36 5.7027363307567 117 37 5.046870174272229 117 38 5.856909886455039 117 39 7.301742430151808 117 40 8.058517549727927 117 41 6.338223566713818 117 42 5.6973741341885 117 43 -2.9547018097108015 117 44 -0.0738759433961853 117 45 6.122682550405333 117 46 -0.33152037559849035 117 47 -0.909142292285992 117 48 1.436912478664997 117 49 0.6833690177248641 117 50 3.0727171971572176 117 51 6.569390104540091 117 52 7.420024878405908 117 53 6.226487011214065 117 54 5.824685008103057 117 55 5.287390236926683 117 56 6.385353147241826 118 1 1.4641940056156102 118 2 0.7644539942862423 118 3 0.9284268077018609 118 4 1.0649744189129606 118 5 1.6127730997215224 118 6 2.357129255049962 118 7 2.3951399861332803 118 8 2.7368876508547118 118 9 3.0432192516254943 118 10 2.2911173907455407 118 11 -0.9670751574430341 118 12 -8.720972020813303 118 13 -10.782226925933333 118 14 -8.65295351227384 118 15 -11.79466031215328 118 16 -8.130976474463935 118 17 -0.4528749965542893 118 18 -7.6925092952517815 118 19 -1.8629544067954455 118 20 -1.798171472779984 118 21 4.830145550348288 118 22 6.233266537661766 118 23 4.916677174766377 118 24 5.731356823440208 118 25 5.706982855419202 118 26 5.691365183635263 118 27 5.547395308603662 118 28 5.8543247239503735 118 29 5.3729160443968205 118 30 6.65108581531814 118 31 5.255844526806143 118 32 5.613258349942978 118 33 5.985339297926771 118 34 5.433742991978185 118 35 5.538611858418413 118 36 6.1464863307567015 118 37 6.953120174272229 118 38 7.825659886455039 118 39 8.957992430151808 118 40 9.339767549727927 118 41 8.519473566713817 118 42 8.628624134188499 118 43 -2.273451809710803 118 44 2.282374056603814 118 45 7.328932550405334 118 46 0.8497296244015082 118 47 0.14085770771400874 118 48 0.9306624786649955 118 49 0.5833690177248627 118 50 3.1039671971572176 118 51 7.106890104540092 118 52 7.463774878405907 118 53 7.388987011214066 118 54 8.480935008103057 118 55 8.249890236926682 118 56 9.935353147241827 119 1 1.3019916246632306 119 2 0.456418280000527 119 3 0.6471768077018609 119 4 0.934022037960581 119 5 1.0755707187691428 119 6 2.296117350288057 119 7 1.9903780813713752 119 8 2.3946257460928067 119 9 3.2098859182921586 119 10 3.2137364383645917 119 11 0.10881769969982358 119 12 -5.134662497003777 119 13 -2.9965126402190485 119 14 -7.745215417035745 119 15 -11.056565074058042 119 16 -10.750024093511556 119 17 -1.143351187030479 119 18 -6.612152152394639 119 19 -0.39420440679544555 119 20 -1.3338857584942687 119 21 3.422407455110193 119 22 4.654397490042715 119 23 3.718760508099713 119 24 1.7953449186783033 119 25 4.494185236371582 119 26 4.718150897920978 119 27 3.633704832413187 119 28 4.631110438236089 119 29 5.0142850920158715 119 30 5.204657243889571 119 31 4.556439764901384 119 32 4.281413111847737 119 33 4.1653988217362965 119 34 5.087016801501996 119 35 3.5639094774660336 119 36 5.743212521232891 119 37 4.540917793319849 119 38 5.023576553121703 119 39 4.718409096818473 119 40 6.987088978299358 119 41 4.2203664238566745 119 42 3.7878503246646886 119 43 -6.173749428758423 119 44 -3.248875943396186 119 45 2.982206359929144 119 46 -4.371996566074682 119 47 -5.323428006571707 119 48 -7.996420854668338 119 49 -3.4761547917989475 119 50 -2.528473279033257 119 51 2.6753424854924717 119 52 2.7628820212630494 119 53 3.4157727254997816 119 54 1.3603992938173413 119 55 3.3123902369266816 119 56 3.5841626710513523 120 1 1.0550370288714248 120 2 -0.061854145248638304 120 3 0.24020006351581458 120 4 0.7800906979827289 120 5 1.2123370532098932 120 6 1.700879255049962 120 7 1.348628358226307 120 8 1.6365969531802946 120 9 2.2641494841836334 120 10 1.852163902373448 120 11 -1.2832088783732658 120 12 -6.425913881278419 120 13 -9.235715298026356 120 14 -8.23580234948314 120 15 -1.1202417075021174 120 16 -9.86353461399882 120 17 -0.8947354616705674 120 18 -10.987567434786666 120 19 -2.475599755632654 120 20 -2.385380775105567 120 21 2.261831596859917 120 22 4.411318863243164 120 23 3.2924039189524237 120 24 3.269874265300672 120 25 3.427186343791295 120 26 3.748051230146892 120 27 4.191290657440874 120 28 4.033103793717817 120 29 5.071317207187516 120 30 4.9621323269460476 120 31 3.632298015178236 120 32 3.461368815059256 120 33 3.118333483973281 120 34 2.8821441547688806 120 35 2.2355595328370157 120 36 4.448085167966006 120 37 4.026521337062924 120 38 4.380165700408529 120 39 5.947091267361113 120 40 4.87247103810002 120 41 -6.68328806119316 120 42 -3.890997958834756 120 43 -8.88028320505964 120 44 -16.004689896884557 120 45 -4.676154658896991 120 46 -9.158264561645003 120 47 -6.725421362053433 120 48 -6.349134032962912 120 49 -14.98058447064723 120 50 -4.3429804772613885 120 51 0.1548552208191616 120 52 0.48048999468497655 120 53 0.6084637554001127 120 54 0.5863129150798017 120 55 0.8705297718104035 120 56 3.9440740774743865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 156.0 5 65.0 6 468.0 7 234.0 8 273.0 9 396.0 10 141.0 11 192.0 12 187.0 13 101.0 14 155.0 15 144.0 16 201.0 17 230.0 18 294.0 19 395.0 20 507.0 21 629.0 22 864.0 23 1057.0 24 1243.0 25 1346.0 26 1538.0 27 1640.0 28 1701.0 29 1879.0 30 2035.0 31 2224.0 32 2518.0 33 2769.0 34 2898.0 35 3156.0 36 3481.0 37 3758.0 38 3414.0 39 2486.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.41261864879955 0.5203796761585706 0.04020100502512563 0.026800670016750423 8 99.43718592964824 0.33500837520938026 0.18313791178112787 0.0446677833612507 9 98.7962032384143 0.5896147403685092 0.3082077051926298 0.30597431602456726 10 51.600223338916805 22.085985482970408 19.200446677833614 7.113344500279174 11 33.45616973757677 35.146845337800116 16.81965382467895 14.577331099944166 12 26.619765494137354 15.271915131211614 19.316582914572862 38.79173646007817 13 21.820212171970965 13.978782802903405 22.635399218313793 41.56560580681184 14 22.005583472920158 18.22892238972641 36.7928531546622 22.972640982691235 15 35.53935860058309 16.225611123570307 23.06122448979592 25.173805786050686 16 40.52556310332499 18.296388988201645 20.296299606721487 20.881748301751877 17 25.892916973798837 16.991668341933032 36.69280082199736 20.422613862270765 18 23.155778894472363 18.13288665549972 35.65159128978224 23.05974316024567 19 38.38453804044241 20.451346218299634 18.855993743715786 22.308121997542173 20 24.53416843753495 33.6450061514372 20.498825634716475 21.321999776311372 21 23.956402296026624 19.36434904965046 34.45603377035267 22.22321488397025 22 21.817978782802903 18.08821887213847 23.2250139586823 36.86878838637633 23 21.882747068676718 19.144611948632047 21.587939698492463 37.384701284198776 24 21.653675206039356 19.20353784646997 34.458267259285726 24.684519688204944 25 37.187849006030824 20.011168192986375 20.817511726602635 21.983471074380166 26 24.65588130139435 19.764479799785484 18.801394351090455 36.77824454772971 27 21.698808911930993 19.725580459898545 19.227245301570985 39.34836532659948 28 22.973667165479196 33.379492104616624 19.656936099881626 23.989904630022558 29 38.63600778401592 20.860267966985035 18.31704207394815 22.186682175050887 30 25.245234956315777 19.587513686233322 34.779791298907334 20.38746005854356 31 25.96282727191493 33.63640425341792 20.317665981592352 20.083102493074794 32 40.974890537038696 19.96023590385131 18.724868197658832 20.340005361451166 33 26.4087656529517 19.226296958855098 19.96422182468694 34.40071556350626 34 22.88073025035237 33.968722733069335 22.840459091214175 20.31008792536412 35 22.02017490885504 21.7562459460064 36.67270571920642 19.55087342593214 36 23.977653631284916 35.27150837988827 20.301675977653634 20.449162011173183 37 24.14448669201521 21.187653768731828 34.14895996421382 20.51889957503914 38 38.05484051482932 22.880805819809737 19.06211527700056 20.00223838836038 39 25.436654366543664 20.758134854075813 18.975735211897575 34.829475567482945 40 20.641634232491167 16.486310153209295 30.04567031879232 32.82638529550721 41 18.407051715586817 14.890211461292214 27.886739708733487 38.815997114387486 42 34.24200340471284 20.49995520114685 21.590807275333752 23.66723411880656 43 19.737166140551192 16.74838247475765 34.72138267624869 28.793068708442476 44 21.576670477215163 19.16257593078251 21.856857866541144 37.40389572546119 45 23.528753636160214 19.480868203177444 34.71022600134258 22.28015215931976 46 21.457199794003717 22.319249457020668 21.893822350596718 34.3297283983789 47 17.80250347705146 29.299205886311636 23.206514424155415 29.691776212481493 48 22.659217877094974 32.00446927374302 23.001117318435753 22.335195530726256 49 20.182192578002596 30.831729262724384 21.760150409597564 27.225927749675456 50 34.09787643491687 19.678220591196936 23.858220143659516 22.365682830226678 51 21.232354918216444 31.922473672417656 25.9041003809097 20.941071028456197 52 21.126350882688563 20.846665025842974 35.68344035978789 22.343543731680576 53 21.793752099428957 19.42447654238047 36.228865748516405 22.552905609674166 54 22.994089728664814 31.279663293633025 23.86719799408973 21.859048983612432 55 20.35667876506699 32.76650087377336 24.29538020343236 22.581440157727293 56 33.38183934807916 19.55986388466016 24.832094564341364 22.226202202919314 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.5 11 3.0 12 6.5 13 23.5 14 37.0 15 48.0 16 59.0 17 61.0 18 63.0 19 51.0 20 39.0 21 36.0 22 38.0 23 43.0 24 45.0 25 47.0 26 58.0 27 69.0 28 71.5 29 99.5 30 125.0 31 139.5 32 154.0 33 179.0 34 204.0 35 250.5 36 297.0 37 351.5 38 454.5 39 503.0 40 545.5 41 588.0 42 671.5 43 755.0 44 913.0 45 1071.0 46 1415.5 47 3340.5 48 4921.0 49 4294.0 50 3667.0 51 3078.5 52 2490.0 53 2390.0 54 2413.5 55 2537.0 56 2622.5 57 2708.0 58 2614.5 59 2521.0 60 2782.0 61 3043.0 62 3120.5 63 2947.0 64 2696.0 65 2402.5 66 2109.0 67 1946.0 68 1783.0 69 1586.0 70 1389.0 71 1244.5 72 885.0 73 670.0 74 626.5 75 583.0 76 429.5 77 276.0 78 208.0 79 129.5 80 119.0 81 127.5 82 136.0 83 89.5 84 43.0 85 27.0 86 11.0 87 7.0 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.41317699609156894 16 0.0513679508654383 17 0.013400335008375211 18 0.0 19 0.0446677833612507 20 0.15633724176437744 21 0.00446677833612507 22 0.0 23 0.0 24 0.00446677833612507 25 0.011166945840312675 26 0.0513679508654383 27 0.0580681183696259 28 0.00446677833612507 29 0.15187046342825236 30 0.04913456169737577 31 0.024567280848687884 32 0.024567280848687884 33 0.12283640424343942 34 0.17420435510887772 35 0.1474036850921273 36 0.05583472920156338 37 0.14517029592406477 38 0.2233389168062535 39 0.1340033500837521 40 0.7280848687883864 41 0.9313232830820771 42 0.2925739810161921 43 0.2412060301507538 44 0.36180904522613067 45 0.1898380792853155 46 0.254606365159129 47 0.43997766610831934 48 0.05583472920156338 49 0.21663874930206586 50 0.192071468453378 51 0.32384142936906757 52 0.18313791178112787 53 0.2680067001675042 54 0.23897264098269125 55 0.3149078726968174 56 0.23897264098269125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 44775.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.07537688442211 #Duplication Level Percentage of deduplicated Percentage of total 1 88.45270202776946 59.32998324958124 2 6.925715046781873 9.290898939140146 3 1.8612859188226285 3.7453936348408714 4 0.7891319548496654 2.117252931323283 5 0.42286817833716245 1.4182021217197096 6 0.2996703626011388 1.2060301507537687 7 0.20643958312522892 0.9692908989391402 8 0.14650551060500117 0.7861529871580123 9 0.09989012086704625 0.6030150753768844 >10 0.719208870242733 8.531546621998883 >50 0.05327473112909134 2.380792853154662 >100 0.016648353477841042 2.3472920156337245 >500 0.003329670695568209 1.4226689000558348 >1k 0.003329670695568209 5.8514796203238415 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 2620 5.8514796203238415 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 637 1.4226689000558348 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 314 0.701284198771636 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 277 0.6186487995533222 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 189 0.4221105527638191 No Hit GTCGGTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 152 0.33947515354550534 No Hit GTCGGTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 119 0.2657733109994417 No Hit GTCGGTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 89 0.1987716359575656 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 86 0.192071468453378 No Hit GTCGGTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 76 0.16973757677275267 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 72 0.16080402010050251 No Hit GTCGGTGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT 71 0.15857063093243998 No Hit GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 70 0.15633724176437744 No Hit GTCGGTGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT 69 0.1541038525963149 No Hit GTCGGTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 67 0.14963707426018982 No Hit GTCGGTGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 67 0.14963707426018982 No Hit GTCGGTGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG 62 0.13847012841987716 No Hit GTCGGTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 61 0.13623673925181462 No Hit GTCGGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 59 0.13176996091568957 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTACG 57 0.1273031825795645 No Hit GTCGGTGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 56 0.12506979341150196 No Hit GTCGGTGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG 53 0.11836962590731435 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 51 0.11390284757118929 No Hit GTCGGTGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG 50 0.11166945840312675 No Hit GTCGGTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC 47 0.10496929089893914 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTAGTCTCAAAGATTAAG 47 0.10496929089893914 No Hit GTCGGTGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG 45 0.10050251256281408 No Hit GTCGGTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA 45 0.10050251256281408 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.00446677833612507 0.0 0.0 0.0 0.0 12 0.00446677833612507 0.0 0.0 0.0 0.0 13 0.00446677833612507 0.0 0.0 0.0 0.0 14 0.00893355667225014 0.0 0.0 0.0 0.0 15 0.00893355667225014 0.0 0.0 0.0 0.0 16 0.011166945840312675 0.0 0.0 0.0 0.0 17 0.011166945840312675 0.0 0.0 0.0 0.0 18 0.011166945840312675 0.0 0.0 0.0 0.0 19 0.011166945840312675 0.0 0.0 0.0 0.0 20 0.011166945840312675 0.0 0.0 0.0 0.0 21 0.011166945840312675 0.0 0.0 0.0 0.0 22 0.011166945840312675 0.0 0.0 0.0 0.0 23 0.011166945840312675 0.0 0.0 0.0 0.0 24 0.011166945840312675 0.0 0.0 0.0 0.0 25 0.015633724176437745 0.0 0.0 0.0 0.0 26 0.02233389168062535 0.0 0.0 0.0 0.0 27 0.024567280848687884 0.0 0.0 0.0 0.0 28 0.02680067001675042 0.0 0.0 0.0 0.0 29 0.03126744835287549 0.0 0.0 0.0 0.0 30 0.03126744835287549 0.0 0.0 0.0 0.0 31 0.03126744835287549 0.0 0.0 0.0 0.0 32 0.03126744835287549 0.0 0.0 0.0 0.0 33 0.03126744835287549 0.0 0.0 0.0 0.0 34 0.03126744835287549 0.0 0.0 0.0 0.0 35 0.03126744835287549 0.0 0.0 0.0 0.0 36 0.03126744835287549 0.0 0.0 0.0 0.0 37 0.03126744835287549 0.0 0.0 0.0 0.0 38 0.03126744835287549 0.0 0.0 0.0 0.0 39 0.03126744835287549 0.0 0.0 0.0 0.0 40 0.03350083752093802 0.0 0.0 0.0 0.0 41 0.03796761585706309 0.0 0.0 0.0 0.0 42 0.03796761585706309 0.0 0.0 0.0 0.0 43 0.0446677833612507 0.0 0.0 0.0 0.0 44 0.04690117252931323 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTAGCC 15 0.00794135 50.68073 39 TCGTCTC 80 0.0 50.68073 40 GTAGCCC 15 0.00794135 50.68073 40 CGTCTCA 95 0.0 50.68073 41 TGCTCGT 85 0.0 50.56542 37 GTAGTAG 15 0.008013301 50.565414 37 ATGCCCG 15 0.008049457 50.507954 36 TGTAGTA 15 0.008049457 50.507954 36 TATGCCC 15 0.008085735 50.450623 35 ATGTAGT 15 0.008085735 50.450623 35 TCAACGA 25 1.9881329E-5 50.279415 45 TCCACGA 15 0.008195299 50.27941 45 TTAAAGA 15 0.008195299 50.27941 45 TAAAGAT 15 0.008232065 50.2226 46 CAGCATT 15 0.008268952 50.165916 43 CAAGATT 70 0.0 50.165913 47 AACGATT 25 2.0149637E-5 50.165913 47 CGCCCAA 35 5.003858E-8 50.165913 47 TTGAACA 20 4.073022E-4 50.165913 43 CTTAAAG 25 2.0284911E-5 50.10936 44 >>END_MODULE