FastQCFastQC Report
Fri 17 Jun 2016
SRR1526041_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526041_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6022
Sequences flagged as poor quality0
Sequence length40
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC490.8136831617402857No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC260.4317502490866822No Hit
GTCGGTGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC210.34872135503155094No Hit
GTCGGTGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGAGC200.33211557622052473No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG120.19926934573231483No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG100.16605778811026237No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTCAGAGC90.14945200929923613No Hit
GTCGGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT80.1328462304882099No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC80.1328462304882099No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG80.1328462304882099No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG70.11624045167718366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGC251.76315E-434.1092456
TGGGGAG301.22458005E-534.1092456
GTGGGGT502.746674E-1034.1092455
GTGGGGC502.746674E-1034.1092455
GGTGGGC502.746674E-1034.1092454
TGGGATC251.76315E-434.1092456
TGGGGTC200.002532756734.109246
TGGGGCC200.002532756734.109246
GTGGGGA700.034.109245
GTGGGAT700.034.109245
GTGGGAC200.002532756734.109245
TGGGATT200.002532756734.109246
TGGGATG200.002532756734.109246
GGTGGGG3100.033.5590934
GTCGGTG6050.032.9816671
TCGGTGG5850.032.9431152
CGGTGGG5750.032.9228363
GTGGGGG1400.032.8910565
TGGGGGG1050.032.4849936
GGTGGGA1700.032.1028184