FastQCFastQC Report
Fri 17 Jun 2016
SRR1526040_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526040_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390
Sequences flagged as poor quality0
Sequence length40
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT70.5035971223021583No Hit
GTCGGTGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.4316546762589928No Hit
GTCGGTGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.3597122302158274No Hit
GTCGGTGGGTACCTGGTTGATCCCCCCCGTACCATATGCT40.28776978417266186No Hit
GTCGGTGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT40.28776978417266186No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG30.2158273381294964No Hit
GTCGGTGGGTCCCTGGTTGATCCTCCCCGTACCCTCTGCT30.2158273381294964No Hit
GTCGGTGGGGGGCTGGTGCGATGGCCCAGTGGGTAAGAGC30.2158273381294964No Hit
GTCGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT30.2158273381294964No Hit
GTCGGTGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.14388489208633093No Hit
GTCGGTGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTT20.14388489208633093No Hit
GTCGGTGGGTACCTGGTTGATCCTCCCCGTCGCCTCTGCT20.14388489208633093No Hit
GTCGGTGGGTCCCTGGTTGATCCTCCCCGTCCCCTCTGCT20.14388489208633093No Hit
GTCGGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT20.14388489208633093No Hit
GTCGGTGGGTACGCAGTTTTTTTTTTTTTTTTTTTTTTTT20.14388489208633093No Hit
GTCGGTGGGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTT20.14388489208633093No Hit
GTCGGTGGGCACGTTTTTTTTTTTTTTTTTTTTTTTTTTT20.14388489208633093No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCCCAGTGGTAGAGCG20.14388489208633093No Hit
GTCGGTGGGGGGGTTGGGGATTTACCTCAGTGGTCGAGCC20.14388489208633093No Hit
GTCGGTGGGTACCTGGTTGACCCCCCCCGTACCCTCTGCT20.14388489208633093No Hit
GTCGGTGGGCTTTCTTTTTTTCCCCCCGGCCTCCTCGCGC20.14388489208633093No Hit
GTCGGTGGGCGTCCCCTTTTTTTTTTTTTTTTTTTTTTTT20.14388489208633093No Hit
GTCGGTGGGTCCCCGGTTGATCCCCCCAGTAGCATATGCT20.14388489208633093No Hit
GTCGGTGGGCTGGTTTGTTTTGTTTTTTTCCTGGGTTGTG20.14388489208633093No Hit
GTCGGTGGGGCGGTTGTGTGTGTATGTGTGTGTGTGTGTG20.14388489208633093No Hit
GTCGGTGGGAATGGTCACCCCCGAGCCGCCTTTTTTTTTT20.14388489208633093No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGGAT251.1477695E-434.05
GGTGGGT200.001917888834.04
GGTGGGG251.1477695E-434.04
GGTGGGC251.1477695E-434.04
TCGGTGG1250.032.642
CGGTGGG1250.032.643
GTCGGTG1250.032.641
GGTGGGA551.3096724E-1030.9090924
Produced by FastQC (version 0.11.5)