##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526040_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1390 Sequences flagged as poor quality 0 Sequence length 40 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.094244604316547 4.0 4.0 26.0 4.0 31.0 2 13.83453237410072 4.0 4.0 26.0 4.0 30.0 3 8.253237410071943 4.0 4.0 11.0 4.0 13.0 4 12.392805755395683 4.0 4.0 22.0 4.0 29.0 5 11.517985611510792 4.0 4.0 21.0 4.0 27.0 6 11.188489208633094 4.0 4.0 20.0 4.0 27.0 7 11.692805755395684 4.0 4.0 22.0 4.0 29.0 8 11.71726618705036 4.0 4.0 23.0 4.0 28.0 9 9.85179856115108 4.0 4.0 17.0 4.0 23.0 10 8.716546762589928 4.0 4.0 15.0 4.0 21.0 11 10.405755395683453 4.0 4.0 16.0 4.0 29.0 12 10.748920863309353 4.0 4.0 16.0 4.0 31.0 13 10.377697841726619 4.0 4.0 16.0 4.0 30.0 14 10.444604316546762 4.0 4.0 15.0 4.0 31.0 15 10.047482014388489 4.0 4.0 12.0 4.0 31.0 16 8.551079136690648 4.0 4.0 6.0 4.0 25.0 17 8.279136690647482 4.0 4.0 4.0 4.0 24.0 18 7.9762589928057555 4.0 4.0 4.0 4.0 22.0 19 7.902158273381295 4.0 4.0 4.0 4.0 22.0 20 7.89136690647482 4.0 4.0 4.0 4.0 22.0 21 7.7870503597122305 4.0 4.0 4.0 4.0 22.0 22 7.787769784172662 4.0 4.0 4.0 4.0 23.0 23 7.854676258992805 4.0 4.0 4.0 4.0 23.0 24 7.560431654676259 4.0 4.0 4.0 4.0 22.0 25 7.6431654676258995 4.0 4.0 4.0 4.0 22.0 26 8.18705035971223 4.0 4.0 4.0 4.0 28.0 27 8.283453237410072 4.0 4.0 4.0 4.0 29.0 28 8.174820143884892 4.0 4.0 4.0 4.0 28.0 29 8.164748201438849 4.0 4.0 4.0 4.0 27.0 30 8.132374100719424 4.0 4.0 4.0 4.0 27.0 31 8.05611510791367 4.0 4.0 4.0 4.0 30.0 32 7.982733812949641 4.0 4.0 4.0 4.0 30.0 33 7.950359712230216 4.0 4.0 4.0 4.0 30.0 34 7.874820143884892 4.0 4.0 4.0 4.0 28.0 35 7.794244604316547 4.0 4.0 4.0 4.0 28.0 36 7.398561151079137 4.0 4.0 4.0 4.0 28.0 37 7.148920863309352 4.0 4.0 4.0 4.0 23.0 38 7.300719424460432 4.0 4.0 4.0 4.0 27.0 39 7.286330935251798 4.0 4.0 4.0 4.0 27.0 40 7.443165467625899 4.0 4.0 4.0 4.0 29.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 17.78972868217054 1 2 -9.586046511627908 1 3 -4.605813953488372 1 4 -8.578294573643413 1 5 -7.758139534883721 1 6 -7.830038759689922 1 7 -7.623643410852713 1 8 -7.006007751937986 1 9 -5.063565891472869 1 10 -3.9217054263565903 1 11 -4.797286821705425 1 12 -4.933333333333334 1 13 -4.608914728682171 1 14 -4.476744186046512 1 15 -4.98953488372093 1 16 -3.876744186046511 1 17 -3.3666666666666663 1 18 -3.3418604651162784 1 19 -3.464341085271318 1 20 -2.703488372093023 1 21 -3.084496124031009 1 22 -3.1697674418604658 1 23 -3.1589147286821717 1 24 -3.253488372093023 1 25 -3.1755813953488374 1 26 -4.197286821705427 1 27 -4.243798449612404 1 28 -4.144961240310078 1 29 -4.274806201550387 1 30 -4.036434108527132 1 31 -4.503875968992249 1 32 -4.3391472868217065 1 33 -4.635658914728683 1 34 -4.565891472868218 1 35 -4.538759689922481 1 36 -3.7422480620155048 1 37 -2.7984496124031004 1 38 -3.550387596899225 1 39 -3.3643410852713185 1 40 -3.4980620155038764 2 1 -5.460271317829459 2 2 0.7472868217054263 2 3 5.727519379844962 2 4 1.7550387596899206 2 5 0.2418604651162788 2 6 -2.8300387596899217 2 7 -3.956976744186046 2 8 -6.339341085271319 2 9 4.603100775193797 2 10 -3.588372093023257 2 11 4.202713178294575 2 12 4.066666666666666 2 13 -4.608914728682171 2 14 -4.476744186046512 2 15 -4.98953488372093 2 16 -3.876744186046511 2 17 -3.3666666666666663 2 18 -3.3418604651162784 2 19 -3.464341085271318 2 20 -2.703488372093023 2 21 -3.084496124031009 2 22 -3.1697674418604658 2 23 -3.1589147286821717 2 24 -3.253488372093023 2 25 -3.1755813953488374 2 26 -4.197286821705427 2 27 -4.243798449612404 2 28 -4.144961240310078 2 29 -4.274806201550387 2 30 -4.036434108527132 2 31 -4.503875968992249 2 32 -4.3391472868217065 2 33 -4.635658914728683 2 34 -4.565891472868218 2 35 -4.538759689922481 2 36 -3.7422480620155048 2 37 -2.7984496124031004 2 38 -3.550387596899225 2 39 -3.3643410852713185 2 40 -3.4980620155038764 6 1 -10.46027131782946 6 2 -9.586046511627908 6 3 -4.605813953488372 6 4 -8.578294573643413 6 5 -7.758139534883721 6 6 -7.830038759689922 6 7 -7.623643410852713 6 8 -7.006007751937986 6 9 -5.063565891472869 6 10 -3.9217054263565903 6 11 -4.797286821705425 6 12 -4.933333333333334 6 13 -4.608914728682171 6 14 -4.476744186046512 6 15 -4.98953488372093 6 16 -3.876744186046511 6 17 -3.3666666666666663 6 18 -3.3418604651162784 6 19 -3.464341085271318 6 20 -2.703488372093023 6 21 -3.084496124031009 6 22 -3.1697674418604658 6 23 -3.1589147286821717 6 24 -3.253488372093023 6 25 -3.1755813953488374 6 26 -4.197286821705427 6 27 -4.243798449612404 6 28 -4.144961240310078 6 29 -4.274806201550387 6 30 -4.036434108527132 6 31 -4.503875968992249 6 32 -4.3391472868217065 6 33 -4.635658914728683 6 34 -4.565891472868218 6 35 -4.538759689922481 6 36 -3.7422480620155048 6 37 -2.7984496124031004 6 38 -3.550387596899225 6 39 -3.3643410852713185 6 40 -3.4980620155038764 8 1 17.03972868217054 8 2 4.413953488372092 8 3 9.394186046511628 8 4 5.921705426356587 8 5 3.241860465116279 8 6 3.6699612403100783 8 7 5.376356589147287 8 8 7.493992248062014 8 9 -5.063565891472869 8 10 -3.9217054263565903 8 11 -4.797286821705425 8 12 -4.933333333333334 8 13 -4.608914728682171 8 14 -4.476744186046512 8 15 -4.98953488372093 8 16 -3.876744186046511 8 17 -3.3666666666666663 8 18 -3.3418604651162784 8 19 -3.464341085271318 8 20 -2.703488372093023 8 21 -3.084496124031009 8 22 -3.1697674418604658 8 23 -3.1589147286821717 8 24 -3.253488372093023 8 25 -3.1755813953488374 8 26 -4.197286821705427 8 27 -4.243798449612404 8 28 -4.144961240310078 8 29 -4.274806201550387 8 30 -4.036434108527132 8 31 -4.503875968992249 8 32 -4.3391472868217065 8 33 -4.635658914728683 8 34 -4.565891472868218 8 35 -4.538759689922481 8 36 -3.7422480620155048 8 37 -2.7984496124031004 8 38 -3.550387596899225 8 39 -3.3643410852713185 8 40 -3.4980620155038764 9 1 14.53972868217054 9 2 11.413953488372092 9 3 2.394186046511628 9 4 2.4217054263565867 9 5 9.241860465116279 9 6 17.16996124031008 9 7 11.376356589147287 9 8 11.993992248062014 9 9 12.93643410852713 9 10 7.07829457364341 9 11 6.202713178294575 9 12 7.066666666666666 9 13 6.391085271317829 9 14 8.523255813953488 9 15 11.01046511627907 9 16 4.123255813953489 9 17 9.633333333333333 9 18 19.658139534883723 9 19 9.535658914728682 9 20 8.296511627906977 9 21 17.91550387596899 9 22 23.830232558139535 9 23 23.84108527131783 9 24 23.746511627906976 9 25 23.824418604651164 9 26 26.802713178294574 9 27 26.756201550387594 9 28 26.855038759689922 9 29 26.725193798449613 9 30 26.963565891472868 9 31 23.496124031007753 9 32 26.660852713178294 9 33 26.364341085271317 9 34 26.434108527131784 9 35 27.46124031007752 9 36 23.257751937984494 9 37 6.2015503875969 9 38 21.449612403100776 9 39 23.63565891472868 9 40 23.501937984496124 10 1 -1.4602713178294593 10 2 0.41395348837209234 10 3 -2.605813953488372 10 4 -1.0782945736434133 10 5 -1.2581395348837212 10 6 0.6699612403100783 10 7 0.37635658914728687 10 8 2.493992248062014 10 9 2.9364341085271306 10 10 1.5782945736434097 10 11 3.702713178294575 10 12 -0.9333333333333336 10 13 1.891085271317829 10 14 -1.9767441860465116 10 15 3.01046511627907 10 16 5.123255813953489 10 17 4.633333333333334 10 18 6.158139534883722 10 19 8.035658914728682 10 20 2.796511627906977 10 21 12.41550387596899 10 22 12.330232558139535 10 23 12.841085271317828 10 24 12.246511627906976 10 25 12.324418604651163 10 26 11.802713178294573 10 27 11.256201550387596 10 28 11.355038759689922 10 29 11.725193798449613 10 30 11.963565891472868 10 31 11.496124031007751 10 32 11.160852713178294 10 33 10.864341085271317 10 34 10.934108527131782 10 35 10.96124031007752 10 36 9.257751937984494 10 37 9.2015503875969 10 38 7.449612403100775 10 39 -0.3643410852713185 10 40 9.501937984496124 11 1 -10.46027131782946 11 2 -9.586046511627908 11 3 -4.605813953488372 11 4 -8.578294573643413 11 5 -7.758139534883721 11 6 -7.830038759689922 11 7 -7.623643410852713 11 8 -7.006007751937986 11 9 -5.063565891472869 11 10 -3.9217054263565903 11 11 -4.797286821705425 11 12 -4.933333333333334 11 13 -4.608914728682171 11 14 -4.476744186046512 11 15 -4.98953488372093 11 16 -3.876744186046511 11 17 -3.3666666666666663 11 18 -3.3418604651162784 11 19 -3.464341085271318 11 20 -2.703488372093023 11 21 -3.084496124031009 11 22 -3.1697674418604658 11 23 -3.1589147286821717 11 24 -3.253488372093023 11 25 -3.1755813953488374 11 26 -4.197286821705427 11 27 -4.243798449612404 11 28 -4.144961240310078 11 29 -4.274806201550387 11 30 -4.036434108527132 11 31 -4.503875968992249 11 32 -4.3391472868217065 11 33 -4.635658914728683 11 34 -4.565891472868218 11 35 -4.538759689922481 11 36 -3.7422480620155048 11 37 -2.7984496124031004 11 38 -3.550387596899225 11 39 -3.3643410852713185 11 40 -3.4980620155038764 12 1 2.5397286821705407 12 2 3.7472868217054245 12 3 0.7275193798449617 12 4 4.755038759689919 12 5 2.241860465116279 12 6 6.169961240310078 12 7 4.709689922480619 12 8 7.660658914728682 12 9 4.603100775193797 12 10 7.07829457364341 12 11 9.536046511627907 12 12 11.733333333333334 12 13 14.724418604651161 12 14 3.8565891472868223 12 15 4.01046511627907 12 16 3.789922480620155 12 17 3.3 12 18 4.658139534883722 12 19 4.535658914728682 12 20 5.963178294573643 12 21 -3.084496124031009 12 22 -3.1697674418604658 12 23 -3.1589147286821717 12 24 -3.253488372093023 12 25 -3.1755813953488374 12 26 -4.197286821705427 12 27 -4.243798449612404 12 28 -4.144961240310078 12 29 -4.274806201550387 12 30 -4.036434108527132 12 31 -4.503875968992249 12 32 -4.3391472868217065 12 33 -4.635658914728683 12 34 -4.565891472868218 12 35 -4.538759689922481 12 36 -3.7422480620155048 12 37 -2.7984496124031004 12 38 -3.550387596899225 12 39 -3.3643410852713185 12 40 -3.4980620155038764 13 1 1.0397286821705407 13 2 1.4139534883720923 13 3 -0.10581395348837219 13 4 0.4217054263565867 13 5 -4.258139534883721 13 6 -0.8300387596899217 13 7 -0.12364341085271313 13 8 2.493992248062014 13 9 0.4364341085271306 13 10 2.0782945736434097 13 11 10.202713178294575 13 12 6.566666666666666 13 13 6.891085271317829 13 14 7.523255813953488 13 15 8.01046511627907 13 16 -3.876744186046511 13 17 -3.3666666666666663 13 18 -3.3418604651162784 13 19 -3.464341085271318 13 20 -2.703488372093023 13 21 -3.084496124031009 13 22 -3.1697674418604658 13 23 -3.1589147286821717 13 24 -3.253488372093023 13 25 -3.1755813953488374 13 26 -4.197286821705427 13 27 -4.243798449612404 13 28 -4.144961240310078 13 29 -4.274806201550387 13 30 -4.036434108527132 13 31 -4.503875968992249 13 32 -4.3391472868217065 13 33 -4.635658914728683 13 34 -4.565891472868218 13 35 -4.538759689922481 13 36 -3.7422480620155048 13 37 -2.7984496124031004 13 38 -3.550387596899225 13 39 -3.3643410852713185 13 40 -3.4980620155038764 14 1 0.03972868217054071 14 2 1.4139534883720923 14 3 -1.1058139534883722 14 4 0.4217054263565867 14 5 -0.2581395348837212 14 6 -1.8300387596899217 14 7 1.8763565891472869 14 8 4.493992248062014 14 9 3.9364341085271306 14 10 4.07829457364341 14 11 9.202713178294575 14 12 9.066666666666666 14 13 7.891085271317829 14 14 11.523255813953488 14 15 4.51046511627907 14 16 12.123255813953488 14 17 13.133333333333333 14 18 12.658139534883722 14 19 12.035658914728682 14 20 13.796511627906977 14 21 5.415503875968991 14 22 -0.6697674418604658 14 23 2.3410852713178283 14 24 10.246511627906976 14 25 3.3244186046511626 14 26 4.802713178294573 14 27 3.256201550387596 14 28 3.855038759689922 14 29 5.725193798449613 14 30 3.963565891472868 14 31 9.996124031007751 14 32 10.160852713178294 14 33 8.364341085271317 14 34 8.434108527131782 14 35 -4.538759689922481 14 36 -3.7422480620155048 14 37 -2.7984496124031004 14 38 -3.550387596899225 14 39 -3.3643410852713185 14 40 -3.4980620155038764 15 1 -10.46027131782946 15 2 -9.586046511627908 15 3 -4.605813953488372 15 4 -8.578294573643413 15 5 -7.758139534883721 15 6 -7.830038759689922 15 7 -7.623643410852713 15 8 -7.006007751937986 15 9 -5.063565891472869 15 10 -3.9217054263565903 15 11 -4.797286821705425 15 12 -4.933333333333334 15 13 -4.608914728682171 15 14 -4.476744186046512 15 15 -4.98953488372093 15 16 -3.876744186046511 15 17 -3.3666666666666663 15 18 -3.3418604651162784 15 19 -3.464341085271318 15 20 -2.703488372093023 15 21 -3.084496124031009 15 22 -3.1697674418604658 15 23 -3.1589147286821717 15 24 -3.253488372093023 15 25 -3.1755813953488374 15 26 -4.197286821705427 15 27 -4.243798449612404 15 28 -4.144961240310078 15 29 -4.274806201550387 15 30 -4.036434108527132 15 31 -4.503875968992249 15 32 -4.3391472868217065 15 33 -4.635658914728683 15 34 -4.565891472868218 15 35 -4.538759689922481 15 36 -3.7422480620155048 15 37 -2.7984496124031004 15 38 -3.550387596899225 15 39 -3.3643410852713185 15 40 -3.4980620155038764 16 1 -10.46027131782946 16 2 -9.586046511627908 16 3 -4.605813953488372 16 4 -8.578294573643413 16 5 -7.758139534883721 16 6 -7.830038759689922 16 7 -7.623643410852713 16 8 -7.006007751937986 16 9 -5.063565891472869 16 10 -3.9217054263565903 16 11 -4.797286821705425 16 12 -4.933333333333334 16 13 -4.608914728682171 16 14 -4.476744186046512 16 15 -4.98953488372093 16 16 -3.876744186046511 16 17 -3.3666666666666663 16 18 -3.3418604651162784 16 19 -3.464341085271318 16 20 -2.703488372093023 16 21 -3.084496124031009 16 22 -3.1697674418604658 16 23 -3.1589147286821717 16 24 -3.253488372093023 16 25 -3.1755813953488374 16 26 -4.197286821705427 16 27 -4.243798449612404 16 28 -4.144961240310078 16 29 -4.274806201550387 16 30 -4.036434108527132 16 31 -4.503875968992249 16 32 -4.3391472868217065 16 33 -4.635658914728683 16 34 -4.565891472868218 16 35 -4.538759689922481 16 36 -3.7422480620155048 16 37 -2.7984496124031004 16 38 -3.550387596899225 16 39 -3.3643410852713185 16 40 -3.4980620155038764 17 1 2.5397286821705407 17 2 -0.08604651162790766 17 3 -2.605813953488372 17 4 3.4217054263565867 17 5 0.7418604651162788 17 6 -0.3300387596899217 17 7 5.876356589147287 17 8 6.493992248062014 17 9 -5.063565891472869 17 10 -3.9217054263565903 17 11 -4.797286821705425 17 12 -4.933333333333334 17 13 -4.608914728682171 17 14 -4.476744186046512 17 15 -4.98953488372093 17 16 -3.876744186046511 17 17 -3.3666666666666663 17 18 -3.3418604651162784 17 19 -3.464341085271318 17 20 -2.703488372093023 17 21 -3.084496124031009 17 22 -3.1697674418604658 17 23 -3.1589147286821717 17 24 -3.253488372093023 17 25 -3.1755813953488374 17 26 -4.197286821705427 17 27 -4.243798449612404 17 28 -4.144961240310078 17 29 -4.274806201550387 17 30 -4.036434108527132 17 31 -4.503875968992249 17 32 -4.3391472868217065 17 33 -4.635658914728683 17 34 -4.565891472868218 17 35 -4.538759689922481 17 36 -3.7422480620155048 17 37 -2.7984496124031004 17 38 -3.550387596899225 17 39 -3.3643410852713185 17 40 -3.4980620155038764 18 1 12.53972868217054 18 2 15.913953488372092 18 3 -0.10581395348837219 18 4 13.421705426356587 18 5 16.74186046511628 18 6 16.16996124031008 18 7 18.876356589147285 18 8 6.493992248062014 18 9 -5.063565891472869 18 10 -3.9217054263565903 18 11 -4.797286821705425 18 12 -4.933333333333334 18 13 -4.608914728682171 18 14 -4.476744186046512 18 15 -4.98953488372093 18 16 -3.876744186046511 18 17 -3.3666666666666663 18 18 -3.3418604651162784 18 19 -3.464341085271318 18 20 -2.703488372093023 18 21 -3.084496124031009 18 22 -3.1697674418604658 18 23 -3.1589147286821717 18 24 -3.253488372093023 18 25 -3.1755813953488374 18 26 -4.197286821705427 18 27 -4.243798449612404 18 28 -4.144961240310078 18 29 -4.274806201550387 18 30 -4.036434108527132 18 31 -4.503875968992249 18 32 -4.3391472868217065 18 33 -4.635658914728683 18 34 -4.565891472868218 18 35 -4.538759689922481 18 36 -3.7422480620155048 18 37 -2.7984496124031004 18 38 -3.550387596899225 18 39 -3.3643410852713185 18 40 -3.4980620155038764 19 1 20.53972868217054 19 2 21.413953488372094 19 3 26.394186046511628 19 4 22.42170542635659 19 5 15.241860465116279 19 6 21.16996124031008 19 7 23.376356589147285 19 8 23.993992248062014 19 9 21.93643410852713 19 10 -3.9217054263565903 19 11 -4.797286821705425 19 12 -4.933333333333334 19 13 -4.608914728682171 19 14 -4.476744186046512 19 15 -4.98953488372093 19 16 -3.876744186046511 19 17 -3.3666666666666663 19 18 -3.3418604651162784 19 19 -3.464341085271318 19 20 -2.703488372093023 19 21 -3.084496124031009 19 22 -3.1697674418604658 19 23 -3.1589147286821717 19 24 -3.253488372093023 19 25 -3.1755813953488374 19 26 -4.197286821705427 19 27 -4.243798449612404 19 28 -4.144961240310078 19 29 -4.274806201550387 19 30 -4.036434108527132 19 31 -4.503875968992249 19 32 -4.3391472868217065 19 33 -4.635658914728683 19 34 -4.565891472868218 19 35 -4.538759689922481 19 36 -3.7422480620155048 19 37 -2.7984496124031004 19 38 -3.550387596899225 19 39 -3.3643410852713185 19 40 -3.4980620155038764 20 1 2.3397286821705414 20 2 2.013953488372092 20 3 -0.4058139534883729 20 4 3.2217054263565874 20 5 4.0418604651162795 20 6 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-3.3643410852713185 109 40 -3.4980620155038764 110 1 8.03972868217054 110 2 12.413953488372092 110 3 3.894186046511628 110 4 13.921705426356587 110 5 3.741860465116279 110 6 0.6699612403100783 110 7 3.876356589147287 110 8 0.993992248062014 110 9 0.9364341085271306 110 10 -3.4217054263565903 110 11 0.7027131782945748 110 12 1.0666666666666664 110 13 4.391085271317829 110 14 6.523255813953488 110 15 4.01046511627907 110 16 3.623255813953489 110 17 -2.3666666666666663 110 18 -1.8418604651162784 110 19 0.5356589147286819 110 20 2.796511627906977 110 21 2.415503875968991 110 22 4.330232558139534 110 23 2.3410852713178283 110 24 6.746511627906977 110 25 0.8244186046511626 110 26 11.302713178294573 110 27 7.256201550387596 110 28 11.355038759689922 110 29 11.225193798449613 110 30 5.963565891472868 110 31 10.996124031007751 110 32 8.160852713178294 110 33 8.864341085271317 110 34 10.934108527131782 110 35 1.9612403100775193 110 36 1.7577519379844952 110 37 2.7015503875968996 110 38 6.449612403100775 110 39 2.1356589147286815 110 40 7.501937984496124 111 1 -10.46027131782946 111 2 -9.586046511627908 111 3 -4.605813953488372 111 4 -8.578294573643413 111 5 -7.758139534883721 111 6 -7.830038759689922 111 7 -7.623643410852713 111 8 -7.006007751937986 111 9 -5.063565891472869 111 10 -3.9217054263565903 111 11 -4.797286821705425 111 12 -4.933333333333334 111 13 -4.608914728682171 111 14 -4.476744186046512 111 15 -4.98953488372093 111 16 -3.876744186046511 111 17 -3.3666666666666663 111 18 -3.3418604651162784 111 19 -3.464341085271318 111 20 -2.703488372093023 111 21 -3.084496124031009 111 22 -3.1697674418604658 111 23 -3.1589147286821717 111 24 -3.253488372093023 111 25 -3.1755813953488374 111 26 -4.197286821705427 111 27 -4.243798449612404 111 28 -4.144961240310078 111 29 -4.274806201550387 111 30 -4.036434108527132 111 31 -4.503875968992249 111 32 -4.3391472868217065 111 33 -4.635658914728683 111 34 -4.565891472868218 111 35 -4.538759689922481 111 36 -3.7422480620155048 111 37 -2.7984496124031004 111 38 -3.550387596899225 111 39 -3.3643410852713185 111 40 -3.4980620155038764 112 1 -10.46027131782946 112 2 -9.586046511627908 112 3 -4.605813953488372 112 4 -8.578294573643413 112 5 -7.758139534883721 112 6 -7.830038759689922 112 7 -7.623643410852713 112 8 -7.006007751937986 112 9 -5.063565891472869 112 10 -3.9217054263565903 112 11 -4.797286821705425 112 12 -4.933333333333334 112 13 -4.608914728682171 112 14 -4.476744186046512 112 15 -4.98953488372093 112 16 -3.876744186046511 112 17 -3.3666666666666663 112 18 -3.3418604651162784 112 19 -3.464341085271318 112 20 -2.703488372093023 112 21 -3.084496124031009 112 22 -3.1697674418604658 112 23 -3.1589147286821717 112 24 -3.253488372093023 112 25 -3.1755813953488374 112 26 -4.197286821705427 112 27 -4.243798449612404 112 28 -4.144961240310078 112 29 -4.274806201550387 112 30 -4.036434108527132 112 31 -4.503875968992249 112 32 -4.3391472868217065 112 33 -4.635658914728683 112 34 -4.565891472868218 112 35 -4.538759689922481 112 36 -3.7422480620155048 112 37 -2.7984496124031004 112 38 -3.550387596899225 112 39 -3.3643410852713185 112 40 -3.4980620155038764 113 1 4.539728682170541 113 2 12.413953488372092 113 3 13.394186046511628 113 4 17.42170542635659 113 5 -7.758139534883721 113 6 -7.830038759689922 113 7 -7.623643410852713 113 8 -7.006007751937986 113 9 -5.063565891472869 113 10 -3.9217054263565903 113 11 -4.797286821705425 113 12 -4.933333333333334 113 13 -4.608914728682171 113 14 -4.476744186046512 113 15 -4.98953488372093 113 16 -3.876744186046511 113 17 -3.3666666666666663 113 18 -3.3418604651162784 113 19 -3.464341085271318 113 20 -2.703488372093023 113 21 -3.084496124031009 113 22 -3.1697674418604658 113 23 -3.1589147286821717 113 24 -3.253488372093023 113 25 -3.1755813953488374 113 26 -4.197286821705427 113 27 -4.243798449612404 113 28 -4.144961240310078 113 29 -4.274806201550387 113 30 -4.036434108527132 113 31 -4.503875968992249 113 32 -4.3391472868217065 113 33 -4.635658914728683 113 34 -4.565891472868218 113 35 -4.538759689922481 113 36 -3.7422480620155048 113 37 -2.7984496124031004 113 38 -3.550387596899225 113 39 -3.3643410852713185 113 40 -3.4980620155038764 114 1 9.03972868217054 114 2 20.413953488372094 114 3 10.894186046511628 114 4 6.921705426356587 114 5 5.741860465116279 114 6 5.169961240310078 114 7 7.876356589147287 114 8 8.493992248062014 114 9 -5.063565891472869 114 10 -3.9217054263565903 114 11 -4.797286821705425 114 12 -4.933333333333334 114 13 -4.608914728682171 114 14 -4.476744186046512 114 15 -4.98953488372093 114 16 -3.876744186046511 114 17 -3.3666666666666663 114 18 -3.3418604651162784 114 19 -3.464341085271318 114 20 -2.703488372093023 114 21 -3.084496124031009 114 22 -3.1697674418604658 114 23 -3.1589147286821717 114 24 -3.253488372093023 114 25 -3.1755813953488374 114 26 -4.197286821705427 114 27 -4.243798449612404 114 28 -4.144961240310078 114 29 -4.274806201550387 114 30 -4.036434108527132 114 31 -4.503875968992249 114 32 -4.3391472868217065 114 33 -4.635658914728683 114 34 -4.565891472868218 114 35 -4.538759689922481 114 36 -3.7422480620155048 114 37 -2.7984496124031004 114 38 -3.550387596899225 114 39 -3.3643410852713185 114 40 -3.4980620155038764 117 1 -10.46027131782946 117 2 -9.586046511627908 117 3 -4.605813953488372 117 4 -8.578294573643413 117 5 -7.758139534883721 117 6 -7.830038759689922 117 7 -7.623643410852713 117 8 -7.006007751937986 117 9 -5.063565891472869 117 10 -3.9217054263565903 117 11 -4.797286821705425 117 12 -4.933333333333334 117 13 -4.608914728682171 117 14 -4.476744186046512 117 15 -4.98953488372093 117 16 -3.876744186046511 117 17 -3.3666666666666663 117 18 -3.3418604651162784 117 19 -3.464341085271318 117 20 -2.703488372093023 117 21 -3.084496124031009 117 22 -3.1697674418604658 117 23 -3.1589147286821717 117 24 -3.253488372093023 117 25 -3.1755813953488374 117 26 -4.197286821705427 117 27 -4.243798449612404 117 28 -4.144961240310078 117 29 -4.274806201550387 117 30 -4.036434108527132 117 31 -4.503875968992249 117 32 -4.3391472868217065 117 33 -4.635658914728683 117 34 -4.565891472868218 117 35 -4.538759689922481 117 36 -3.7422480620155048 117 37 -2.7984496124031004 117 38 -3.550387596899225 117 39 -3.3643410852713185 117 40 -3.4980620155038764 118 1 16.53972868217054 118 2 -9.586046511627908 118 3 -4.605813953488372 118 4 -8.578294573643413 118 5 -7.758139534883721 118 6 -7.830038759689922 118 7 -7.623643410852713 118 8 -7.006007751937986 118 9 -5.063565891472869 118 10 -3.9217054263565903 118 11 -4.797286821705425 118 12 -4.933333333333334 118 13 -4.608914728682171 118 14 -4.476744186046512 118 15 -4.98953488372093 118 16 -3.876744186046511 118 17 -3.3666666666666663 118 18 -3.3418604651162784 118 19 -3.464341085271318 118 20 -2.703488372093023 118 21 -3.084496124031009 118 22 -3.1697674418604658 118 23 -3.1589147286821717 118 24 -3.253488372093023 118 25 -3.1755813953488374 118 26 -4.197286821705427 118 27 -4.243798449612404 118 28 -4.144961240310078 118 29 -4.274806201550387 118 30 -4.036434108527132 118 31 -4.503875968992249 118 32 -4.3391472868217065 118 33 -4.635658914728683 118 34 -4.565891472868218 118 35 -4.538759689922481 118 36 -3.7422480620155048 118 37 -2.7984496124031004 118 38 -3.550387596899225 118 39 -3.3643410852713185 118 40 -3.4980620155038764 120 1 -10.46027131782946 120 2 -9.586046511627908 120 3 -4.605813953488372 120 4 -8.578294573643413 120 5 -7.758139534883721 120 6 -7.830038759689922 120 7 -7.623643410852713 120 8 -7.006007751937986 120 9 -5.063565891472869 120 10 -3.9217054263565903 120 11 -4.797286821705425 120 12 -4.933333333333334 120 13 -4.608914728682171 120 14 -4.476744186046512 120 15 -4.98953488372093 120 16 -3.876744186046511 120 17 -3.3666666666666663 120 18 -3.3418604651162784 120 19 -3.464341085271318 120 20 -2.703488372093023 120 21 -3.084496124031009 120 22 -3.1697674418604658 120 23 -3.1589147286821717 120 24 -3.253488372093023 120 25 -3.1755813953488374 120 26 -4.197286821705427 120 27 -4.243798449612404 120 28 -4.144961240310078 120 29 -4.274806201550387 120 30 -4.036434108527132 120 31 -4.503875968992249 120 32 -4.3391472868217065 120 33 -4.635658914728683 120 34 -4.565891472868218 120 35 -4.538759689922481 120 36 -3.7422480620155048 120 37 -2.7984496124031004 120 38 -3.550387596899225 120 39 -3.3643410852713185 120 40 -3.4980620155038764 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 779.0 5 30.0 6 39.0 7 23.0 8 23.0 9 29.0 10 44.0 11 58.0 12 30.0 13 24.0 14 27.0 15 20.0 16 10.0 17 14.0 18 14.0 19 22.0 20 31.0 21 38.0 22 38.0 23 23.0 24 29.0 25 11.0 26 15.0 27 10.0 28 6.0 29 2.0 30 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 98.18971759594497 0.724112961622013 0.724112961622013 0.3620564808110065 8 97.62589928057554 0.6474820143884892 0.7913669064748201 0.935251798561151 9 96.11231101511879 1.2239020878329734 1.2239020878329734 1.4398848092152627 10 37.84172661870503 26.834532374100718 16.618705035971225 18.705035971223023 11 30.935251798561154 25.68345323741007 20.431654676258994 22.949640287769785 12 37.05035971223021 9.928057553956835 23.52517985611511 29.496402877697843 13 27.41007194244604 13.741007194244602 25.179856115107913 33.669064748201436 14 19.150467962562995 16.05471562275018 37.79697624190065 26.997840172786177 15 32.51798561151079 12.733812949640289 33.45323741007194 21.294964028776977 16 34.53237410071942 11.942446043165468 29.856115107913666 23.66906474820144 17 26.258992805755394 14.388489208633093 34.53237410071942 24.820143884892087 18 22.014388489208635 17.050359712230215 34.10071942446043 26.834532374100718 19 21.949458483754512 16.750902527075812 26.209386281588447 35.09025270758123 20 20.359712230215827 23.52517985611511 28.561151079136692 27.55395683453237 21 20.215827338129497 17.769784172661872 34.388489208633096 27.62589928057554 22 17.050359712230215 18.633093525179856 30.35971223021583 33.9568345323741 23 20.014398848092153 12.958963282937367 28.293736501079913 38.73290136789057 24 18.77697841726619 18.27338129496403 29.928057553956833 33.02158273381295 25 21.77361211247296 13.987022350396538 25.018024513338137 39.221341023792355 26 17.435158501440924 14.048991354466859 27.737752161383284 40.778097982708935 27 18.596237337192477 13.603473227206948 32.63386396526773 35.166425470332854 28 16.930835734870318 14.697406340057636 29.178674351585016 39.19308357348703 29 21.645021645021643 14.285714285714285 32.25108225108225 31.818181818181817 30 18.070554355651545 12.8869690424766 40.8207343412527 28.22174226061915 31 14.316546762589926 16.258992805755394 37.697841726618705 31.726618705035968 32 18.848920863309353 10.359712230215827 35.39568345323741 35.39568345323741 33 16.65465032444124 7.7865897620764235 39.36553713049747 36.193222782984854 34 13.381294964028779 12.661870503597122 43.7410071942446 30.215827338129497 35 11.366906474820144 12.51798561151079 50.50359712230216 25.611510791366904 36 13.453237410071942 18.1294964028777 44.172661870503596 24.244604316546763 37 12.274368231046932 14.007220216606498 47.942238267148014 25.776173285198556 38 18.992805755395683 13.16546762589928 43.16546762589928 24.676258992805757 39 14.820143884892087 11.870503597122301 43.597122302158276 29.71223021582734 40 13.597122302158274 12.086330935251798 48.70503597122302 25.611510791366904 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 2.0 21 6.5 22 11.0 23 11.0 24 11.5 25 12.0 26 20.0 27 28.0 28 28.0 29 30.0 30 32.0 31 33.0 32 34.0 33 34.0 34 32.0 35 30.0 36 34.0 37 38.0 38 38.0 39 36.5 40 35.0 41 38.0 42 41.0 43 41.0 44 39.0 45 37.0 46 50.0 47 63.0 48 63.0 49 74.5 50 86.0 51 84.5 52 83.0 53 83.0 54 81.5 55 80.0 56 79.5 57 79.0 58 79.0 59 94.5 60 110.0 61 112.0 62 114.0 63 114.0 64 109.5 65 105.0 66 100.5 67 96.0 68 96.0 69 87.5 70 79.0 71 67.0 72 55.0 73 55.0 74 52.0 75 49.0 76 46.0 77 43.0 78 43.0 79 31.5 80 20.0 81 14.5 82 9.0 83 9.0 84 10.0 85 11.0 86 8.0 87 5.0 88 5.0 89 3.0 90 1.0 91 1.5 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.6474820143884892 8 0.0 9 0.07194244604316546 10 0.0 11 0.0 12 0.0 13 0.0 14 0.07194244604316546 15 0.0 16 0.0 17 0.0 18 0.0 19 0.3597122302158274 20 0.0 21 0.0 22 0.0 23 0.07194244604316546 24 0.0 25 0.2158273381294964 26 0.14388489208633093 27 0.5755395683453237 28 0.14388489208633093 29 0.28776978417266186 30 0.07194244604316546 31 0.0 32 0.0 33 0.2158273381294964 34 0.0 35 0.0 36 0.0 37 0.3597122302158274 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1390.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.69064748201438 #Duplication Level Percentage of deduplicated Percentage of total 1 98.06547619047619 94.82014388489208 2 1.2648809523809523 2.446043165467626 3 0.2976190476190476 0.8633093525179856 4 0.1488095238095238 0.5755395683453237 5 0.0744047619047619 0.3597122302158274 6 0.0744047619047619 0.4316546762589928 7 0.0744047619047619 0.5035971223021583 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCGGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.5035971223021583 No Hit GTCGGTGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.4316546762589928 No Hit GTCGGTGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.3597122302158274 No Hit GTCGGTGGGTACCTGGTTGATCCCCCCCGTACCATATGCT 4 0.28776978417266186 No Hit GTCGGTGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT 4 0.28776978417266186 No Hit GTCGGTGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG 3 0.2158273381294964 No Hit GTCGGTGGGTCCCTGGTTGATCCTCCCCGTACCCTCTGCT 3 0.2158273381294964 No Hit GTCGGTGGGGGGCTGGTGCGATGGCCCAGTGGGTAAGAGC 3 0.2158273381294964 No Hit GTCGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 3 0.2158273381294964 No Hit GTCGGTGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.14388489208633093 No Hit GTCGGTGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTT 2 0.14388489208633093 No Hit GTCGGTGGGTACCTGGTTGATCCTCCCCGTCGCCTCTGCT 2 0.14388489208633093 No Hit GTCGGTGGGTCCCTGGTTGATCCTCCCCGTCCCCTCTGCT 2 0.14388489208633093 No Hit GTCGGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT 2 0.14388489208633093 No Hit GTCGGTGGGTACGCAGTTTTTTTTTTTTTTTTTTTTTTTT 2 0.14388489208633093 No Hit GTCGGTGGGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTT 2 0.14388489208633093 No Hit GTCGGTGGGCACGTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.14388489208633093 No Hit GTCGGTGGGGGGGTTGGGGATTTAGCCCAGTGGTAGAGCG 2 0.14388489208633093 No Hit GTCGGTGGGGGGGTTGGGGATTTACCTCAGTGGTCGAGCC 2 0.14388489208633093 No Hit GTCGGTGGGTACCTGGTTGACCCCCCCCGTACCCTCTGCT 2 0.14388489208633093 No Hit GTCGGTGGGCTTTCTTTTTTTCCCCCCGGCCTCCTCGCGC 2 0.14388489208633093 No Hit GTCGGTGGGCGTCCCCTTTTTTTTTTTTTTTTTTTTTTTT 2 0.14388489208633093 No Hit GTCGGTGGGTCCCCGGTTGATCCCCCCAGTAGCATATGCT 2 0.14388489208633093 No Hit GTCGGTGGGCTGGTTTGTTTTGTTTTTTTCCTGGGTTGTG 2 0.14388489208633093 No Hit GTCGGTGGGGCGGTTGTGTGTGTATGTGTGTGTGTGTGTG 2 0.14388489208633093 No Hit GTCGGTGGGAATGGTCACCCCCGAGCCGCCTTTTTTTTTT 2 0.14388489208633093 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGGGAT 25 1.1477695E-4 34.0 5 GGTGGGT 20 0.0019178888 34.0 4 GGTGGGG 25 1.1477695E-4 34.0 4 GGTGGGC 25 1.1477695E-4 34.0 4 TCGGTGG 125 0.0 32.64 2 CGGTGGG 125 0.0 32.64 3 GTCGGTG 125 0.0 32.64 1 GGTGGGA 55 1.3096724E-10 30.909092 4 >>END_MODULE