FastQCFastQC Report
Fri 17 Jun 2016
SRR1526039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3865
Sequences flagged as poor quality0
Sequence length40
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT2867.399741267787839No Hit
GTCGGTGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT350.9055627425614489No Hit
GTCGGTGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT330.8538163001293662No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC320.8279430789133247No Hit
GTCGGTGGGTACCTGGTTGATACTGCCAGTAGCATATGCT140.36222509702457956No Hit
GTCGGTGGGTACATGGTTGATCCTGCCAGTAGCATATGCT130.33635187580853815No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG110.28460543337645533No Hit
GTCGGTGGGTACCTGGTTGACCCTGCCAGTAGCATATGCT110.28460543337645533No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC90.2328589909443726No Hit
GTCGGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT80.2069857697283312No Hit
GTCGGTGGGGGGGAGGTAGTGACGAAAAATAACAATACAG80.2069857697283312No Hit
GTCGGTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC80.2069857697283312No Hit
GTCGGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.18111254851228978No Hit
GTCGGTGGGTAAATGGTTGATCCTGCCAGTAGCATATGCT60.15523932729624837No Hit
GTCGGTGGGCAGTGCCTTCCCAACCATTCCCATTGGCCTC60.15523932729624837No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC50.129366106080207No Hit
GTCGGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.129366106080207No Hit
GTCGGTGGGAACTGTGGTAATTCTAGAGCTAATACATGCC50.129366106080207No Hit
GTCGGTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGC50.129366106080207No Hit
GTCGGTGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT50.129366106080207No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATTGCTT50.129366106080207No Hit
GTCGGTGGGGGGGTGGCTGAAGGCGAAGATGACTTTATTG40.1034928848641656No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG40.1034928848641656No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGC40.1034928848641656No Hit
GTCGGTGGGTCCCTGGCTGATCCTGCCAGTAGCATATGCT40.1034928848641656No Hit
GTCGGTGGGACGGCCGGTACAGTGAAACTGCGAATGGCTC40.1034928848641656No Hit
GTCGGTGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT40.1034928848641656No Hit
GTCGGTGGGATTCGTAGGAGTAAACATAACATTCTTCCCT40.1034928848641656No Hit
GTCGGTGGGTCCCTGGTTGCTCCTGCCAGTAGCATATGCT40.1034928848641656No Hit
GTCGGTGGGATTTACAGTTGGTGGTCTAACCGGAATTGTT40.1034928848641656No Hit
GTCGGTGGGCCTAATCCTAGCCCTAGCCCTACACAAATAT40.1034928848641656No Hit
GTCGGTGGGTAACTGGTTGATACTGCCAGTAGCATATGCT40.1034928848641656No Hit
GTCGGTGGGCTGCCGTGTCTCTGCAACAAAGCATTCTGGG40.1034928848641656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGGGG1000.034.0000045
GTGGGCG251.6783574E-434.0000045
GTGGGAG251.6783574E-434.0000045
GGTGGGA900.034.0000044
TGGGGGG600.034.06
GGGGGGG200.00245937134.07
GTGGGCT200.00245937134.05
GTGGGAT405.0926246E-834.05
GGGGGAA200.00245937134.08
ATATGCT357.6436845E-734.034
TGGGTAC301.1371412E-534.06
GGTGGGT551.4551915E-1134.04
GGTGGGC600.034.04
TGGGATT200.00245937134.06
GTCGGTG3950.033.139241
TCGGTGG3900.033.1282042
CGGTGGG3900.033.1282043
GGTGGGG1850.032.162164
GCCAGTA451.4360012E-730.22222325
TGCCAGT451.4360012E-730.22222324
Produced by FastQC (version 0.11.5)