##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526039_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3865 Sequences flagged as poor quality 0 Sequence length 40 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.2137128072445 33.0 30.0 34.0 4.0 36.0 2 20.917205692108666 24.0 4.0 33.0 4.0 36.0 3 19.446571798188874 23.0 4.0 30.0 4.0 35.0 4 19.415523932729624 23.0 4.0 31.0 4.0 35.0 5 18.084346701164296 18.0 4.0 31.0 4.0 35.0 6 14.667011642949547 4.0 4.0 28.0 4.0 33.0 7 14.26908150064683 4.0 4.0 29.0 4.0 33.0 8 13.354721862871928 4.0 4.0 28.0 4.0 33.0 9 10.518499353169469 4.0 4.0 20.0 4.0 28.0 10 10.172833117723156 4.0 4.0 17.0 4.0 28.0 11 9.454851228978008 4.0 4.0 11.0 4.0 27.0 12 9.685640362225097 4.0 4.0 14.0 4.0 28.0 13 9.221733505821474 4.0 4.0 10.0 4.0 27.0 14 9.385510996119017 4.0 4.0 10.0 4.0 29.0 15 9.210608020698578 4.0 4.0 4.0 4.0 28.0 16 8.229754204398448 4.0 4.0 4.0 4.0 26.0 17 7.842173350582147 4.0 4.0 4.0 4.0 23.0 18 7.788357050452781 4.0 4.0 4.0 4.0 24.0 19 7.366106080206986 4.0 4.0 4.0 4.0 21.0 20 7.21216041397154 4.0 4.0 4.0 4.0 20.0 21 7.115653298835705 4.0 4.0 4.0 4.0 20.0 22 7.005692108667529 4.0 4.0 4.0 4.0 20.0 23 7.057179818887452 4.0 4.0 4.0 4.0 20.0 24 6.947994825355757 4.0 4.0 4.0 4.0 19.0 25 7.19353169469599 4.0 4.0 4.0 4.0 22.0 26 7.292367399741268 4.0 4.0 4.0 4.0 22.0 27 7.3203104786545925 4.0 4.0 4.0 4.0 22.0 28 7.390426908150065 4.0 4.0 4.0 4.0 23.0 29 7.03104786545925 4.0 4.0 4.0 4.0 20.0 30 7.333247089262613 4.0 4.0 4.0 4.0 23.0 31 7.630271668822768 4.0 4.0 4.0 4.0 25.0 32 7.665200517464425 4.0 4.0 4.0 4.0 26.0 33 7.4297542043984475 4.0 4.0 4.0 4.0 24.0 34 7.560155239327297 4.0 4.0 4.0 4.0 26.0 35 7.344890038809832 4.0 4.0 4.0 4.0 23.0 36 7.477360931435964 4.0 4.0 4.0 4.0 26.0 37 7.1640362225097025 4.0 4.0 4.0 4.0 22.0 38 7.200776196636482 4.0 4.0 4.0 4.0 23.0 39 6.978525226390686 4.0 4.0 4.0 4.0 20.0 40 6.964553686934023 4.0 4.0 4.0 4.0 19.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 5.83580246913581 2 2 -3.0205246913580197 2 3 2.666203703703701 2 4 0.8282407407407426 2 5 4.795987654320989 2 6 -2.757716049382715 2 7 -1.6493827160493844 2 8 -0.7192901234567906 2 9 -5.528086419753086 2 10 -1.6450617283950617 2 11 -1.398919753086421 2 12 -1.1884259259259258 2 13 -0.41450617283950475 2 14 -0.6574074074074074 2 15 0.37453703703703844 2 16 -0.47700617283950475 2 17 -0.8162037037037031 2 18 1.7919753086419767 2 19 -0.1702160493827174 2 20 -1.1370370370370386 2 21 0.7472222222222218 2 22 -0.27484567901234414 2 23 1.2186728395061754 2 24 -1.261111111111111 2 25 0.7929012345679025 2 26 1.5293209876543212 2 27 1.7969135802469136 2 28 1.160956790123456 2 29 0.4861111111111107 2 30 0.187808641975308 2 31 -1.457716049382717 2 32 0.5118827160493815 2 33 0.2851851851851839 2 34 2.1984567901234566 2 35 -0.44135802469135754 2 36 2.44861111111111 2 37 -2.4373456790123447 2 38 -2.5891975308641975 2 39 -2.5220679012345677 2 40 -2.3875 3 1 6.83580246913581 3 2 2.0461419753086467 3 3 11.43287037037037 3 4 9.19490740740741 3 5 6.462654320987657 3 6 -2.524382716049381 3 7 -3.3493827160493836 3 8 4.347376543209878 3 9 2.405246913580248 3 10 0.9216049382716047 3 11 4.5344135802469125 3 12 2.1782407407407405 3 13 3.9521604938271615 3 14 -0.6240740740740742 3 15 5.407870370370372 3 16 5.156327160493829 3 17 3.917129629629631 3 18 3.2253086419753103 3 19 4.329783950617283 3 20 5.3962962962962955 3 21 7.813888888888888 3 22 6.125154320987656 3 23 6.552006172839509 3 24 4.805555555555555 3 25 3.4929012345679027 3 26 4.795987654320987 3 27 8.330246913580247 3 28 8.960956790123458 3 29 5.6194444444444445 3 30 7.521141975308642 3 31 10.308950617283951 3 32 10.07854938271605 3 33 11.118518518518517 3 34 6.831790123456791 3 35 5.025308641975308 3 36 8.54861111111111 3 37 11.729320987654322 3 38 7.4108024691358025 3 39 11.311265432098764 3 40 9.945833333333333 4 1 -0.6641975308641896 4 2 -3.370524691358021 4 3 6.766203703703702 4 4 2.528240740740742 4 5 -2.9540123456790113 4 6 4.392283950617285 4 7 -4.849382716049384 4 8 -1.3192901234567902 4 9 0.07191358024691397 4 10 1.2549382716049386 4 11 -4.798919753086421 4 12 -4.988425925925926 4 13 -4.214506172839505 4 14 -4.457407407407407 4 15 -4.425462962962962 4 16 -3.677006172839505 4 17 -3.4162037037037027 4 18 -3.6080246913580236 4 19 -3.1702160493827174 4 20 -2.9370370370370384 4 21 -2.852777777777778 4 22 -2.874845679012344 4 23 -2.7813271604938246 4 24 -2.8611111111111107 4 25 -3.0070987654320973 4 26 -2.870679012345679 4 27 -3.003086419753087 4 28 -3.039043209876543 4 29 -2.7138888888888895 4 30 -2.812191358024692 4 31 -2.8577160493827174 4 32 -3.088117283950618 4 33 -2.714814814814816 4 34 -3.0015432098765427 4 35 -2.6413580246913577 4 36 -2.95138888888889 4 37 -2.4373456790123447 4 38 -2.5891975308641975 4 39 -2.5220679012345677 4 40 -2.3875 5 1 4.33580246913581 5 2 6.046141975308647 5 3 0.2662037037037024 5 4 9.19490740740741 5 5 2.629320987654321 5 6 4.642283950617285 5 7 -4.01604938271605 5 8 -3.4859567901234563 5 9 -1.428086419753086 5 10 -5.245061728395061 5 11 -4.798919753086421 5 12 -4.988425925925926 5 13 -4.214506172839505 5 14 -4.457407407407407 5 15 -4.425462962962962 5 16 -3.677006172839505 5 17 -3.4162037037037027 5 18 -3.6080246913580236 5 19 -3.1702160493827174 5 20 -2.9370370370370384 5 21 -2.852777777777778 5 22 -2.874845679012344 5 23 -2.7813271604938246 5 24 -2.8611111111111107 5 25 -3.0070987654320973 5 26 -2.870679012345679 5 27 -3.003086419753087 5 28 -3.039043209876543 5 29 -2.7138888888888895 5 30 -2.812191358024692 5 31 -2.8577160493827174 5 32 -3.088117283950618 5 33 -2.714814814814816 5 34 -3.0015432098765427 5 35 -2.6413580246913577 5 36 -2.95138888888889 5 37 -2.4373456790123447 5 38 -2.5891975308641975 5 39 -2.5220679012345677 5 40 -2.3875 6 1 1.0858024691358104 6 2 2.629475308641979 6 3 -10.733796296296298 6 4 -0.2217592592592581 6 5 8.545987654320989 6 6 -2.8577160493827147 6 7 9.150617283950616 6 8 16.93070987654321 6 9 14.571913580246914 6 10 5.754938271604939 6 11 8.201080246913579 6 12 8.261574074074074 6 13 11.535493827160495 6 14 10.542592592592593 6 15 15.074537037037038 6 16 22.572993827160495 6 17 17.5837962962963 6 18 17.391975308641975 6 19 7.579783950617283 6 20 13.562962962962962 6 21 9.397222222222222 6 22 10.625154320987656 6 23 10.468672839506176 6 24 13.38888888888889 6 25 10.242901234567903 6 26 7.129320987654321 6 27 9.746913580246913 6 28 12.460956790123458 6 29 11.036111111111111 6 30 11.187808641975309 6 31 14.642283950617283 6 32 16.411882716049384 6 33 10.535185185185185 6 34 11.498456790123457 6 35 16.358641975308643 6 36 15.79861111111111 6 37 16.312654320987654 6 38 17.160802469135803 6 39 19.727932098765432 6 40 21.3625 7 1 3.8358024691358104 7 2 8.379475308641979 7 3 7.516203703703702 7 4 11.778240740740742 7 5 9.545987654320989 7 6 0.6422839506172853 7 7 11.150617283950616 7 8 19.68070987654321 7 9 15.071913580246914 7 10 15.754938271604939 7 11 9.201080246913579 7 12 17.261574074074076 7 13 17.785493827160494 7 14 8.542592592592593 7 15 14.574537037037038 7 16 16.072993827160495 7 17 13.333796296296297 7 18 16.641975308641975 7 19 17.829783950617283 7 20 8.062962962962962 7 21 16.147222222222222 7 22 13.875154320987656 7 23 11.218672839506176 7 24 8.63888888888889 7 25 12.242901234567903 7 26 13.62932098765432 7 27 12.996913580246913 7 28 12.710956790123458 7 29 8.786111111111111 7 30 12.937808641975309 7 31 19.142283950617283 7 32 19.161882716049384 7 33 19.035185185185185 7 34 19.998456790123456 7 35 20.108641975308643 7 36 17.29861111111111 7 37 15.812654320987654 7 38 16.410802469135803 7 39 17.477932098765432 7 40 16.6125 8 1 5.83580246913581 8 2 0.712808641975311 8 3 11.93287037037037 8 4 10.861574074074074 8 5 4.795987654320989 8 6 19.47561728395062 8 7 14.150617283950616 8 8 12.18070987654321 8 9 12.738580246913582 8 10 11.921604938271606 8 11 5.701080246913579 8 12 5.011574074074074 8 13 6.118827160493829 8 14 3.3759259259259267 8 15 4.407870370370372 8 16 1.156327160493829 8 17 -0.9162037037037027 8 18 0.3919753086419764 8 19 -0.33688271604938436 8 20 -1.7703703703703715 8 21 -2.186111111111111 8 22 -0.8748456790123438 8 23 -1.6146604938271576 8 24 -2.6944444444444438 8 25 -0.6737654320987643 8 26 -1.8706790123456791 8 27 0.33024691358024594 8 28 -1.8723765432098762 8 29 -0.0472222222222225 8 30 0.854475308641975 8 31 -1.5243827160493844 8 32 1.7452160493827158 8 33 0.45185185185185084 8 34 0.1651234567901243 8 35 -1.3080246913580247 8 36 2.3819444444444438 8 37 2.2293209876543214 8 38 2.5774691358024686 8 39 2.8112654320987662 8 40 2.279166666666666 9 1 5.83580246913581 9 2 10.629475308641979 9 3 8.516203703703702 9 4 3.778240740740742 9 5 -1.2040123456790113 9 6 13.392283950617285 9 7 -1.8493827160493836 9 8 -5.06929012345679 9 9 -1.928086419753086 9 10 1.5049382716049386 9 11 -2.5489197530864214 9 12 3.2615740740740744 9 13 2.2854938271604954 9 14 2.7925925925925927 9 15 -0.42546296296296227 9 16 2.322993827160495 9 17 -1.1662037037037027 9 18 3.1419753086419764 9 19 -0.9202160493827174 9 20 3.5629629629629616 9 21 0.8972222222222221 9 22 -0.12484567901234378 9 23 -0.7813271604938246 9 24 2.3888888888888893 9 25 1.4929012345679027 9 26 -1.3706790123456791 9 27 3.246913580246913 9 28 -0.7890432098765432 9 29 2.2861111111111105 9 30 3.687808641975308 9 31 4.392283950617283 9 32 4.161882716049382 9 33 4.535185185185184 9 34 4.248456790123457 9 35 4.608641975308642 9 36 5.54861111111111 9 37 6.062654320987655 9 38 5.9108024691358025 9 39 5.477932098765432 9 40 5.6125 10 1 0.23580246913580893 10 2 -2.620524691358021 10 3 1.066203703703703 10 4 -0.3717592592592567 10 5 -0.004012345679011986 10 6 -3.557716049382714 10 7 0.750617283950616 10 8 1.4807098765432105 10 9 0.6719135802469136 10 10 3.7549382716049386 10 11 2.0010802469135793 10 12 3.811574074074075 10 13 4.585493827160496 10 14 5.742592592592592 10 15 4.574537037037038 10 16 5.722993827160495 10 17 5.183796296296297 10 18 4.191975308641977 10 19 4.829783950617283 10 20 8.862962962962962 10 21 5.947222222222223 10 22 7.525154320987657 10 23 4.418672839506175 10 24 8.53888888888889 10 25 6.792901234567903 10 26 7.32932098765432 10 27 7.996913580246913 10 28 7.960956790123457 10 29 2.0861111111111112 10 30 0.3878086419753082 10 31 2.1422839506172826 10 32 0.5118827160493815 10 33 2.6851851851851842 10 34 -3.0015432098765427 10 35 -2.6413580246913577 10 36 -2.95138888888889 10 37 -2.4373456790123447 10 38 -2.5891975308641975 10 39 -2.5220679012345677 10 40 -2.3875 11 1 5.435802469135812 11 2 -6.620524691358021 11 3 10.466203703703702 11 4 12.828240740740743 11 5 13.995987654320988 11 6 1.242283950617285 11 7 4.950617283950617 11 8 -5.91929012345679 11 9 -3.128086419753086 11 10 -2.4450617283950615 11 11 1.601080246913579 11 12 2.811574074074075 11 13 0.5854938271604961 11 14 3.3425925925925934 11 15 -1.225462962962962 11 16 -1.0770061728395053 11 17 0.9837962962962976 11 18 -2.808024691358024 11 19 -1.770216049382717 11 20 -2.1370370370370386 11 21 1.1472222222222221 11 22 -0.4748456790123434 11 23 1.2186728395061754 11 24 1.5388888888888896 11 25 0.5929012345679023 11 26 2.32932098765432 11 27 2.196913580246912 11 28 1.9609567901234568 11 29 3.0861111111111112 11 30 3.187808641975308 11 31 2.1422839506172826 11 32 3.111882716049381 11 33 3.0851851851851846 11 34 2.3984567901234577 11 35 -2.6413580246913577 11 36 -2.95138888888889 11 37 -2.4373456790123447 11 38 -2.5891975308641975 11 39 -2.5220679012345677 11 40 -2.3875 12 1 5.08580246913581 12 2 8.879475308641979 12 3 8.516203703703702 12 4 3.028240740740742 12 5 10.295987654320989 12 6 1.6422839506172853 12 7 15.150617283950616 12 8 11.18070987654321 12 9 6.321913580246914 12 10 7.004938271604939 12 11 1.2010802469135786 12 12 0.2615740740740744 12 13 7.785493827160495 12 14 9.292592592592593 12 15 7.324537037037038 12 16 10.072993827160495 12 17 9.083796296296297 12 18 9.391975308641976 12 19 6.579783950617283 12 20 0.31296296296296156 12 21 10.897222222222222 12 22 7.875154320987656 12 23 8.218672839506176 12 24 11.13888888888889 12 25 10.992901234567903 12 26 16.87932098765432 12 27 13.496913580246913 12 28 11.710956790123458 12 29 15.786111111111111 12 30 18.93780864197531 12 31 13.892283950617283 12 32 17.661882716049384 12 33 12.035185185185185 12 34 11.248456790123457 12 35 9.358641975308643 12 36 19.04861111111111 12 37 6.062654320987655 12 38 8.910802469135803 12 39 6.977932098765432 12 40 6.8625 13 1 5.235802469135809 13 2 9.379475308641979 13 3 4.666203703703701 13 4 7.028240740740742 13 5 0.7959876543209887 13 6 0.6422839506172853 13 7 13.150617283950616 13 8 14.080709876543208 13 9 14.071913580246914 13 10 7.35493827160494 13 11 10.601080246913577 13 12 6.611574074074074 13 13 5.185493827160496 13 14 8.942592592592591 13 15 5.774537037037037 13 16 9.322993827160495 13 17 7.383796296296298 13 18 8.191975308641977 13 19 10.229783950617282 13 20 11.46296296296296 13 21 6.147222222222222 13 22 5.725154320987656 13 23 11.218672839506176 13 24 6.338888888888889 13 25 9.192901234567902 13 26 10.52932098765432 13 27 3.7969135802469136 13 28 8.160956790123457 13 29 7.686111111111111 13 30 6.387808641975307 13 31 5.542283950617283 13 32 2.111882716049381 13 33 4.8851851851851835 13 34 5.998456790123457 13 35 8.958641975308641 13 36 4.6486111111111095 13 37 2.162654320987655 13 38 5.210802469135803 13 39 2.6779320987654316 13 40 -2.3875 14 1 4.83580246913581 14 2 2.712808641975311 14 3 -2.4004629629629655 14 4 4.361574074074074 14 5 6.129320987654321 14 6 -9.357716049382715 14 7 -8.849382716049384 14 8 -8.31929012345679 14 9 -5.928086419753086 14 10 -5.245061728395061 14 11 -4.798919753086421 14 12 -4.988425925925926 14 13 -4.214506172839505 14 14 -4.457407407407407 14 15 -4.425462962962962 14 16 -3.677006172839505 14 17 -3.4162037037037027 14 18 -3.6080246913580236 14 19 -3.1702160493827174 14 20 -2.9370370370370384 14 21 -2.852777777777778 14 22 -2.874845679012344 14 23 -2.7813271604938246 14 24 -2.8611111111111107 14 25 -3.0070987654320973 14 26 -2.870679012345679 14 27 -3.003086419753087 14 28 -3.039043209876543 14 29 -2.7138888888888895 14 30 -2.812191358024692 14 31 -2.8577160493827174 14 32 -3.088117283950618 14 33 -2.714814814814816 14 34 -3.0015432098765427 14 35 -2.6413580246913577 14 36 -2.95138888888889 14 37 -2.4373456790123447 14 38 -2.5891975308641975 14 39 -2.5220679012345677 14 40 -2.3875 15 1 4.1691358024691425 15 2 4.712808641975311 15 3 -7.400462962962964 15 4 13.361574074074074 15 5 3.4626543209876566 15 6 -2.6910493827160487 15 7 1.4839506172839503 15 8 2.0140432098765437 15 9 2.73858024691358 15 10 3.4216049382716047 15 11 -4.798919753086421 15 12 -4.988425925925926 15 13 -4.214506172839505 15 14 -4.457407407407407 15 15 -4.425462962962962 15 16 -3.677006172839505 15 17 -3.4162037037037027 15 18 -3.6080246913580236 15 19 -3.1702160493827174 15 20 -2.9370370370370384 15 21 -2.852777777777778 15 22 -2.874845679012344 15 23 -2.7813271604938246 15 24 -2.8611111111111107 15 25 -3.0070987654320973 15 26 -2.870679012345679 15 27 -3.003086419753087 15 28 -3.039043209876543 15 29 -2.7138888888888895 15 30 -2.812191358024692 15 31 -2.8577160493827174 15 32 -3.088117283950618 15 33 -2.714814814814816 15 34 -3.0015432098765427 15 35 -2.6413580246913577 15 36 -2.95138888888889 15 37 -2.4373456790123447 15 38 -2.5891975308641975 15 39 -2.5220679012345677 15 40 -2.3875 16 1 -11.16419753086419 16 2 -4.620524691358021 16 3 -8.333796296296297 16 4 -2.3717592592592585 16 5 -2.2040123456790113 16 6 3.2422839506172867 16 7 -6.249382716049384 16 8 -1.9192901234567898 16 9 -1.1280864197530853 16 10 -2.045061728395061 16 11 1.401080246913578 16 12 -2.7884259259259254 16 13 -0.6145061728395049 16 14 -0.6574074074074074 16 15 1.3745370370370384 16 16 -0.47700617283950475 16 17 0.9837962962962976 16 18 1.7919753086419767 16 19 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111 29 3.2861111111111105 111 30 3.437808641975308 111 31 4.392283950617283 111 32 4.411882716049382 111 33 5.285185185185184 111 34 5.248456790123457 111 35 5.608641975308642 111 36 4.29861111111111 111 37 5.062654320987655 111 38 -0.5891975308641975 111 39 3.9779320987654323 111 40 1.8624999999999998 112 1 -7.16419753086419 112 2 -7.953858024691355 112 3 -6.0671296296296315 112 4 -13.971759259259258 112 5 -13.204012345679011 112 6 -9.357716049382715 112 7 -8.849382716049384 112 8 -8.31929012345679 112 9 -5.928086419753086 112 10 -5.245061728395061 112 11 -4.798919753086421 112 12 -4.988425925925926 112 13 -4.214506172839505 112 14 -4.457407407407407 112 15 -4.425462962962962 112 16 -3.677006172839505 112 17 -3.4162037037037027 112 18 -3.6080246913580236 112 19 -3.1702160493827174 112 20 -2.9370370370370384 112 21 -2.852777777777778 112 22 -2.874845679012344 112 23 -2.7813271604938246 112 24 -2.8611111111111107 112 25 -3.0070987654320973 112 26 -2.870679012345679 112 27 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113 26 -2.870679012345679 113 27 -3.003086419753087 113 28 -3.039043209876543 113 29 -2.7138888888888895 113 30 -2.812191358024692 113 31 -2.8577160493827174 113 32 -3.088117283950618 113 33 -2.714814814814816 113 34 -3.0015432098765427 113 35 -2.6413580246913577 113 36 -2.95138888888889 113 37 -2.4373456790123447 113 38 -2.5891975308641975 113 39 -2.5220679012345677 113 40 -2.3875 114 1 -3.9141975308641896 114 2 -2.620524691358021 114 3 -6.483796296296298 114 4 -7.721759259259258 114 5 0.2959876543209887 114 6 -8.107716049382715 114 7 -3.0993827160493836 114 8 -6.31929012345679 114 9 -5.178086419753086 114 10 -0.9950617283950614 114 11 0.7010802469135786 114 12 1.7615740740740744 114 13 2.5354938271604954 114 14 -4.457407407407407 114 15 -4.425462962962962 114 16 -3.677006172839505 114 17 -3.4162037037037027 114 18 -3.6080246913580236 114 19 -3.1702160493827174 114 20 -2.9370370370370384 114 21 -2.852777777777778 114 22 -2.874845679012344 114 23 -2.7813271604938246 114 24 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23 4.218672839506175 115 24 -0.19444444444444375 115 25 5.659567901234569 115 26 2.795987654320987 115 27 0.6635802469135799 115 28 1.9609567901234568 115 29 -0.7138888888888895 115 30 1.187808641975308 115 31 0.47561728395061564 115 32 1.2452160493827158 115 33 -1.381481481481483 115 34 3.6651234567901234 115 35 2.691975308641976 115 36 7.381944444444444 115 37 6.229320987654321 115 38 7.7441358024691365 115 39 4.811265432098766 115 40 7.945833333333334 116 1 4.83580246913581 116 2 0.37947530864197887 116 3 1.2662037037037024 116 4 -4.305092592592592 116 5 -13.204012345679011 116 6 -9.357716049382715 116 7 -8.849382716049384 116 8 -8.31929012345679 116 9 -5.928086419753086 116 10 -5.245061728395061 116 11 -4.798919753086421 116 12 -4.988425925925926 116 13 -4.214506172839505 116 14 -4.457407407407407 116 15 -4.425462962962962 116 16 -3.677006172839505 116 17 -3.4162037037037027 116 18 -3.6080246913580236 116 19 -3.1702160493827174 116 20 -2.9370370370370384 116 21 -2.852777777777778 116 22 -2.874845679012344 116 23 -2.7813271604938246 116 24 -2.8611111111111107 116 25 -3.0070987654320973 116 26 -2.870679012345679 116 27 -3.003086419753087 116 28 -3.039043209876543 116 29 -2.7138888888888895 116 30 -2.812191358024692 116 31 -2.8577160493827174 116 32 -3.088117283950618 116 33 -2.714814814814816 116 34 -3.0015432098765427 116 35 -2.6413580246913577 116 36 -2.95138888888889 116 37 -2.4373456790123447 116 38 -2.5891975308641975 116 39 -2.5220679012345677 116 40 -2.3875 117 1 6.502469135802475 117 2 9.712808641975311 117 3 9.93287037037037 117 4 13.361574074074074 117 5 11.462654320987657 117 6 7.308950617283953 117 7 10.817283950617284 117 8 19.68070987654321 117 9 10.405246913580246 117 10 0.4216049382716047 117 11 2.5344135802469125 117 12 4.011574074074074 117 13 -4.214506172839505 117 14 -4.457407407407407 117 15 -4.425462962962962 117 16 -3.677006172839505 117 17 -3.4162037037037027 117 18 -3.6080246913580236 117 19 -3.1702160493827174 117 20 -2.9370370370370384 117 21 -2.852777777777778 117 22 -2.874845679012344 117 23 -2.7813271604938246 117 24 -2.8611111111111107 117 25 -3.0070987654320973 117 26 -2.870679012345679 117 27 -3.003086419753087 117 28 -3.039043209876543 117 29 -2.7138888888888895 117 30 -2.812191358024692 117 31 -2.8577160493827174 117 32 -3.088117283950618 117 33 -2.714814814814816 117 34 -3.0015432098765427 117 35 -2.6413580246913577 117 36 -2.95138888888889 117 37 -2.4373456790123447 117 38 -2.5891975308641975 117 39 -2.5220679012345677 117 40 -2.3875 119 1 9.58580246913581 119 2 15.129475308641979 119 3 13.766203703703702 119 4 11.528240740740742 119 5 19.29598765432099 119 6 11.142283950617285 119 7 -0.5993827160493836 119 8 6.18070987654321 119 9 -5.928086419753086 119 10 -5.245061728395061 119 11 -4.798919753086421 119 12 -4.988425925925926 119 13 -4.214506172839505 119 14 -4.457407407407407 119 15 -4.425462962962962 119 16 -3.677006172839505 119 17 -3.4162037037037027 119 18 -3.6080246913580236 119 19 -3.1702160493827174 119 20 -2.9370370370370384 119 21 -2.852777777777778 119 22 -2.874845679012344 119 23 -2.7813271604938246 119 24 -2.8611111111111107 119 25 -3.0070987654320973 119 26 -2.870679012345679 119 27 -3.003086419753087 119 28 -3.039043209876543 119 29 -2.7138888888888895 119 30 -2.812191358024692 119 31 -2.8577160493827174 119 32 -3.088117283950618 119 33 -2.714814814814816 119 34 -3.0015432098765427 119 35 -2.6413580246913577 119 36 -2.95138888888889 119 37 -2.4373456790123447 119 38 -2.5891975308641975 119 39 -2.5220679012345677 119 40 -2.3875 120 1 2.3358024691358104 120 2 12.879475308641979 120 3 6.266203703703702 120 4 15.028240740740742 120 5 1.2959876543209887 120 6 5.142283950617285 120 7 -8.849382716049384 120 8 -8.31929012345679 120 9 -5.928086419753086 120 10 -5.245061728395061 120 11 -4.798919753086421 120 12 -4.988425925925926 120 13 -4.214506172839505 120 14 -4.457407407407407 120 15 -4.425462962962962 120 16 -3.677006172839505 120 17 -3.4162037037037027 120 18 -3.6080246913580236 120 19 -3.1702160493827174 120 20 -2.9370370370370384 120 21 -2.852777777777778 120 22 -2.874845679012344 120 23 -2.7813271604938246 120 24 -2.8611111111111107 120 25 -3.0070987654320973 120 26 -2.870679012345679 120 27 -3.003086419753087 120 28 -3.039043209876543 120 29 -2.7138888888888895 120 30 -2.812191358024692 120 31 -2.8577160493827174 120 32 -3.088117283950618 120 33 -2.714814814814816 120 34 -3.0015432098765427 120 35 -2.6413580246913577 120 36 -2.95138888888889 120 37 -2.4373456790123447 120 38 -2.5891975308641975 120 39 -2.5220679012345677 120 40 -2.3875 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 1084.0 5 242.0 6 362.0 7 473.0 8 309.0 9 175.0 10 122.0 11 131.0 12 83.0 13 71.0 14 56.0 15 46.0 16 29.0 17 31.0 18 29.0 19 31.0 20 66.0 21 102.0 22 91.0 23 99.0 24 67.0 25 76.0 26 39.0 27 22.0 28 12.0 29 11.0 30 1.0 31 3.0 32 1.0 33 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.35266701191092 0.38839979285344384 0.12946659761781462 0.12946659761781462 8 98.62836438923395 0.7246376811594203 0.36231884057971014 0.2846790890269151 9 98.47189847189847 0.5439005439005439 0.5180005180005179 0.4662004662004662 10 44.01864319005696 23.69238736406007 20.688762299326775 11.600207146556189 11 32.45341614906832 31.599378881987576 20.21221532091097 15.734989648033126 12 28.700828157349896 19.72049689440994 20.781573498964804 30.79710144927536 13 22.095730918499353 18.21474773609314 24.890038809831825 34.79948253557568 14 19.534282018111256 22.716688227684347 40.67270375161708 17.076326002587322 15 37.98188874514877 21.164294954721864 25.071151358344114 15.782664941785251 16 42.50970245795602 21.707632600258734 20.7503234152652 15.032341526520051 17 22.897800776196636 20.905562742561447 40.310478654592494 15.88615782664942 18 23.053040103492886 21.707632600258734 40.80206985769728 14.437257438551098 19 37.03416149068323 25.698757763975156 22.308488612836438 14.958592132505174 20 24.553686934023286 36.972833117723155 23.751617076326003 14.721862871927554 21 21.241914618369986 26.39068564036223 36.66235446313066 15.705045278137128 22 19.79301423027167 22.147477360931436 26.934023285899094 31.1254851228978 23 20.056935817805382 23.576604554865423 24.611801242236027 31.754658385093165 24 21.241914618369986 22.19922380336352 38.08538163001294 18.47347994825356 25 35.57742102537546 23.84774728120145 21.80217503883998 18.772656654583116 26 21.44153487166191 25.097225823178636 20.637801400051853 32.823437905107596 27 18.748377044923398 23.39652038431576 22.69540379122306 35.159698779537784 28 19.306418219461698 40.13975155279503 21.11801242236025 19.435817805383024 29 35.86278586278586 25.571725571725572 20.47817047817048 18.08731808731809 30 19.746376811594203 23.9648033126294 39.90683229813665 16.38198757763975 31 21.48033126293996 37.96583850931677 23.75776397515528 16.79606625258799 32 38.3916990920882 22.801556420233464 22.30869001297017 16.49805447470817 33 22.664941785252264 23.751617076326003 21.707632600258734 31.875808538163003 34 18.36998706338939 38.08538163001294 26.64941785252264 16.895213454075034 35 17.335058214747736 27.529107373868044 39.45666235446313 15.679172056921086 36 17.552501944516465 41.32745657246565 24.241638579206636 16.878402903811253 37 20.853816300129367 25.304010349288486 37.69728331177232 16.14489003880983 38 33.11772315653299 27.34799482535576 22.43208279430789 17.102199223803364 39 21.966364812419144 25.459249676584733 21.914618369987064 30.659767141009052 40 18.91332470892626 22.690815006468306 37.723156532988355 20.672703751617078 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 2.0 17 4.0 18 4.0 19 7.5 20 11.0 21 15.5 22 20.0 23 20.0 24 17.5 25 15.0 26 16.5 27 18.0 28 18.0 29 21.5 30 25.0 31 29.0 32 33.0 33 33.0 34 42.5 35 52.0 36 70.5 37 89.0 38 89.0 39 108.0 40 127.0 41 149.0 42 171.0 43 171.0 44 193.5 45 216.0 46 245.0 47 274.0 48 274.0 49 293.5 50 313.0 51 365.5 52 418.0 53 418.0 54 524.0 55 630.0 56 508.5 57 387.0 58 387.0 59 336.5 60 286.0 61 295.5 62 305.0 63 305.0 64 282.0 65 259.0 66 188.0 67 117.0 68 117.0 69 79.0 70 41.0 71 33.0 72 25.0 73 25.0 74 19.5 75 14.0 76 10.5 77 7.0 78 7.0 79 6.5 80 6.0 81 3.5 82 1.0 83 1.0 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.07761966364812418 8 0.0258732212160414 9 0.1034928848641656 10 0.07761966364812418 11 0.0258732212160414 12 0.0258732212160414 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0258732212160414 20 0.0 21 0.0 22 0.0 23 0.0258732212160414 24 0.0 25 0.07761966364812418 26 0.2069857697283312 27 0.36222509702457956 28 0.0258732212160414 29 0.4398447606727037 30 0.0258732212160414 31 0.0258732212160414 32 0.258732212160414 33 0.0 34 0.0 35 0.0 36 0.2069857697283312 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 3865.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.76714100905563 #Duplication Level Percentage of deduplicated Percentage of total 1 92.11806681803438 73.47994825355757 2 5.514109633473889 8.796895213454075 3 1.2974375608173856 3.1047865459249677 4 0.3892312682452157 1.241914618369987 5 0.19461563412260785 0.7761966364812419 6 0.06487187804086927 0.31047865459249674 7 0.03243593902043464 0.18111254851228978 8 0.09730781706130393 0.6209573091849935 9 0.03243593902043464 0.2328589909443726 >10 0.22705157314304247 3.855109961190168 >50 0.0 0.0 >100 0.03243593902043464 7.399741267787839 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 286 7.399741267787839 No Hit GTCGGTGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT 35 0.9055627425614489 No Hit GTCGGTGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT 33 0.8538163001293662 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 32 0.8279430789133247 No Hit GTCGGTGGGTACCTGGTTGATACTGCCAGTAGCATATGCT 14 0.36222509702457956 No Hit GTCGGTGGGTACATGGTTGATCCTGCCAGTAGCATATGCT 13 0.33635187580853815 No Hit GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 11 0.28460543337645533 No Hit GTCGGTGGGTACCTGGTTGACCCTGCCAGTAGCATATGCT 11 0.28460543337645533 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC 9 0.2328589909443726 No Hit GTCGGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 8 0.2069857697283312 No Hit GTCGGTGGGGGGGAGGTAGTGACGAAAAATAACAATACAG 8 0.2069857697283312 No Hit GTCGGTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC 8 0.2069857697283312 No Hit GTCGGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.18111254851228978 No Hit GTCGGTGGGTAAATGGTTGATCCTGCCAGTAGCATATGCT 6 0.15523932729624837 No Hit GTCGGTGGGCAGTGCCTTCCCAACCATTCCCATTGGCCTC 6 0.15523932729624837 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC 5 0.129366106080207 No Hit GTCGGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.129366106080207 No Hit GTCGGTGGGAACTGTGGTAATTCTAGAGCTAATACATGCC 5 0.129366106080207 No Hit GTCGGTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGC 5 0.129366106080207 No Hit GTCGGTGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 5 0.129366106080207 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATTGCTT 5 0.129366106080207 No Hit GTCGGTGGGGGGGTGGCTGAAGGCGAAGATGACTTTATTG 4 0.1034928848641656 No Hit GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG 4 0.1034928848641656 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGC 4 0.1034928848641656 No Hit GTCGGTGGGTCCCTGGCTGATCCTGCCAGTAGCATATGCT 4 0.1034928848641656 No Hit GTCGGTGGGACGGCCGGTACAGTGAAACTGCGAATGGCTC 4 0.1034928848641656 No Hit GTCGGTGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT 4 0.1034928848641656 No Hit GTCGGTGGGATTCGTAGGAGTAAACATAACATTCTTCCCT 4 0.1034928848641656 No Hit GTCGGTGGGTCCCTGGTTGCTCCTGCCAGTAGCATATGCT 4 0.1034928848641656 No Hit GTCGGTGGGATTTACAGTTGGTGGTCTAACCGGAATTGTT 4 0.1034928848641656 No Hit GTCGGTGGGCCTAATCCTAGCCCTAGCCCTACACAAATAT 4 0.1034928848641656 No Hit GTCGGTGGGTAACTGGTTGATACTGCCAGTAGCATATGCT 4 0.1034928848641656 No Hit GTCGGTGGGCTGCCGTGTCTCTGCAACAAAGCATTCTGGG 4 0.1034928848641656 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGGGGG 100 0.0 34.000004 5 GTGGGCG 25 1.6783574E-4 34.000004 5 GTGGGAG 25 1.6783574E-4 34.000004 5 GGTGGGA 90 0.0 34.000004 4 TGGGGGG 60 0.0 34.0 6 GGGGGGG 20 0.002459371 34.0 7 GTGGGCT 20 0.002459371 34.0 5 GTGGGAT 40 5.0926246E-8 34.0 5 GGGGGAA 20 0.002459371 34.0 8 ATATGCT 35 7.6436845E-7 34.0 34 TGGGTAC 30 1.1371412E-5 34.0 6 GGTGGGT 55 1.4551915E-11 34.0 4 GGTGGGC 60 0.0 34.0 4 TGGGATT 20 0.002459371 34.0 6 GTCGGTG 395 0.0 33.13924 1 TCGGTGG 390 0.0 33.128204 2 CGGTGGG 390 0.0 33.128204 3 GGTGGGG 185 0.0 32.16216 4 GCCAGTA 45 1.4360012E-7 30.222223 25 TGCCAGT 45 1.4360012E-7 30.222223 24 >>END_MODULE