FastQCFastQC Report
Fri 17 Jun 2016
SRR1526038_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526038_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12799
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1411.1016485662942417No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC450.35158996796624736No Hit
CTGTGTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT440.34377685756699744No Hit
CTGTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG420.32815063676849754No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC300.23439331197749824No Hit
CTGTGTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA280.21876709117899834No Hit
CTGTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG280.21876709117899834No Hit
CTGTGTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT250.19532775998124854No Hit
CTGTGTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC240.1875146495819986No Hit
CTGTGTGGGATGGCTCGTGTGCAGCGTAGATCTATCACATGTGGAAGTGTGTATAC240.1875146495819986No Hit
CTGTGTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC230.17970153918274867No Hit
CTGTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG220.17188842878349872No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC180.14063598718649895No Hit
CTGTGTGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT170.132822876787249No Hit
CTGTGTGGGGACTTTGTTCTCTTAAGAAAACCCTGGAATTGTTCTGTTTCCCCAGC160.12500976638799907No Hit
CTGTGTGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC160.12500976638799907No Hit
CTGTGTGGGGGAGACATGATCCTTTTGTGTCTGGCAGGCCTCATGCAACAAATTTT160.12500976638799907No Hit
CTGTGTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC150.11719665598874912No Hit
CTGTGTGGGAGGGCGTGGGAGCTGTGGTCACGGCATGCATCTCAGTGAAAGAAGAT130.10157043519024925No Hit
CTGTGTGGGGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA130.10157043519024925No Hit
CTGTGTGGGGGGACTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC130.10157043519024925No Hit
CTGTGTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAGGAGCACTGACTGCTCTTCG130.10157043519024925No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATGT150.00813466550.16535636
ACATGTG150.00813466550.16535637
TATGCTC150.00813466550.16535635
CCTGCCG150.00813466550.16535640
ATGTGGA150.00813466550.16535639
TGCTCGT150.00813466550.16535637
ATGCTCG150.00813466550.16535636
TCGTCTC150.00813466550.16535640
CGTGCAT150.00813466550.16535641
ATCACAT150.00813466550.16535634
TGTGGAA150.00813466550.16535640
CTGCCGT150.00813466550.16535641
TCACATG150.00813466550.16535635
CATGTGG150.00813466550.16535638
GTTGATC451.1641532E-1049.9686316
GTACCTG451.1641532E-1049.968639
ACCTGGT451.1641532E-1049.9686311
TGGGGTG451.1641532E-1049.968636
GTGGGAT750.049.968635
TTGATCC451.1641532E-1049.9686317
Produced by FastQC (version 0.11.5)