##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526038_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12799 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.092350964919135 38.0 38.0 40.0 35.0 40.0 2 37.44394093288538 38.0 38.0 40.0 34.0 40.0 3 36.93046331744667 38.0 36.0 40.0 33.0 40.0 4 36.828814751152436 38.0 35.0 40.0 32.0 40.0 5 36.758496757559186 38.0 36.0 40.0 32.0 40.0 6 36.1791546214548 38.0 35.0 40.0 30.0 40.0 7 37.39339010860223 38.0 38.0 40.0 34.0 40.0 8 37.13407297445113 38.0 38.0 40.0 33.0 40.0 9 37.17032580670365 38.0 38.0 40.0 33.0 40.0 10 35.63083053363544 38.0 35.0 40.0 29.0 40.0 11 34.49456988827252 38.0 32.0 40.0 26.0 40.0 12 33.873036956012186 38.0 32.0 40.0 22.0 40.0 13 33.6270802406438 38.0 32.0 39.0 20.0 40.0 14 34.38034221423549 38.0 33.0 39.0 24.0 40.0 15 33.507539651535275 38.0 32.0 39.0 19.0 40.0 16 33.138213922962734 38.0 31.0 39.0 20.0 40.0 17 32.1479021798578 36.0 29.0 39.0 16.0 40.0 18 31.35698101414173 36.0 27.0 39.0 14.0 40.0 19 32.54183920618798 37.0 29.0 39.0 18.0 40.0 20 33.26134854285491 38.0 31.0 39.0 22.0 40.0 21 33.18626455191812 38.0 31.0 39.0 19.0 40.0 22 32.36276271583718 37.0 29.0 39.0 16.0 40.0 23 33.07688100632862 38.0 30.0 39.0 19.0 40.0 24 32.40135948120947 37.0 29.0 39.0 17.0 40.0 25 32.849519493710446 37.0 30.0 39.0 19.0 40.0 26 33.52269708570982 38.0 33.0 39.0 19.0 40.0 27 33.67739667161497 38.0 33.0 39.0 21.0 40.0 28 33.72075943433081 38.0 33.0 39.0 22.0 40.0 29 33.223376826314556 38.0 32.0 39.0 19.0 40.0 30 32.92569732010313 37.0 31.0 39.0 18.0 40.0 31 33.845847331822796 38.0 33.0 39.0 22.0 40.0 32 33.72341589186655 38.0 33.0 39.0 21.0 40.0 33 33.59043675287132 38.0 33.0 39.0 21.0 40.0 34 33.41909524181577 38.0 33.0 39.0 19.0 40.0 35 33.267755293382294 38.0 33.0 39.0 19.0 40.0 36 32.68067817798266 37.0 31.0 39.0 18.0 40.0 37 32.398781154777716 36.0 31.0 39.0 16.0 40.0 38 31.79334322993984 36.0 30.0 39.0 10.0 40.0 39 30.89967966247363 35.0 29.0 38.0 4.0 40.0 40 28.543870614891787 34.0 22.0 38.0 4.0 40.0 41 26.96843503398703 32.0 17.0 38.0 4.0 40.0 42 27.968356902883038 33.0 23.0 38.0 4.0 40.0 43 27.802250175794985 33.0 22.0 38.0 4.0 40.0 44 26.871786858348308 32.0 19.0 38.0 4.0 39.0 45 27.93593249472615 33.0 23.0 38.0 4.0 39.0 46 25.776388780373466 30.0 17.0 36.0 4.0 39.0 47 24.777873271349325 29.0 12.0 36.0 4.0 39.0 48 25.54019845300414 30.0 17.0 36.0 4.0 39.0 49 24.522462692397845 29.0 10.0 36.0 4.0 39.0 50 25.0978982733026 30.0 15.0 36.0 4.0 39.0 51 25.301195405891086 30.0 13.0 36.0 4.0 39.0 52 25.205875459020238 30.0 10.0 36.0 4.0 39.0 53 24.949605437924838 30.0 4.0 36.0 4.0 39.0 54 24.69927338073287 30.0 4.0 36.0 4.0 39.0 55 24.30572700992265 30.0 4.0 36.0 4.0 39.0 56 23.73990155480897 30.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.4145945592262379 1 2 0.7592389885842934 1 3 1.538958916654174 1 4 0.5717788450130001 1 5 1.9781639380113774 1 6 2.2430043274535265 1 7 1.726574848244077 1 8 1.4403062859476918 1 9 1.1508759553065815 1 10 3.0385734841024075 1 11 -2.7447162451181377 1 12 -9.655920061712354 1 13 -18.222214745049975 1 14 -7.784254491436059 1 15 -1.3531939379226898 1 16 -0.16180915200870416 1 17 -6.573975823565906 1 18 -4.669187568809779 1 19 -0.4897498180525517 1 20 0.5185164401317657 1 21 5.757915944393346 1 22 6.640216280328936 1 23 5.165061753109686 1 24 5.352541237855473 1 25 4.265872204959475 1 26 2.851762439678332 1 27 3.779804879736478 1 28 3.7396288857160833 1 29 3.5293152914533223 1 30 6.082312268586033 1 31 3.8192015918297244 1 32 4.273049488026047 1 33 2.034311048693276 1 34 4.96156203318052 1 35 4.253008712555879 1 36 3.5045577772629706 1 37 5.232037646008941 1 38 4.689904641514257 1 39 3.642394948708585 1 40 -9.104263737488942 1 41 0.9511961068534518 1 42 -0.017798969103530737 1 43 -5.99380594445968 1 44 -13.16451457202803 1 45 -0.4864689278545846 1 46 -3.8453760023376127 1 47 -5.554019241699532 1 48 -2.8812438241853755 1 49 -9.431738859227032 1 50 -0.4230881092379981 1 51 7.321554918670206 1 52 6.605991311675112 1 53 3.7994485654969488 1 54 6.641349353598869 1 55 6.060940042435831 1 56 7.585019706578798 2 1 1.685694303471763 2 2 2.6773975563592316 2 3 3.165557382127318 2 4 3.316024369309673 2 5 3.4845577999039605 2 6 4.26602223026682 2 7 2.7623804748425442 2 8 2.788132372904215 2 9 2.6189066458436656 2 10 4.943944328092172 2 11 3.091600890431735 2 12 -1.057454588566575 2 13 2.015636917354122 2 14 4.0546201888708495 2 15 5.334785601719254 2 16 4.807500310906899 2 17 7.507865608659156 2 18 6.034137239374363 2 19 6.781349926192977 2 20 5.531304163916932 2 21 7.704207504495649 2 22 8.514896587234304 2 23 7.264805998633985 2 24 8.797554025579256 2 25 7.749248164038757 2 26 7.601123053489069 2 27 6.992081094570242 2 28 7.297173642749332 2 29 7.925734728793472 2 30 7.678220196974781 2 31 6.340940722264506 2 32 6.776885805161598 2 33 5.384694680406831 2 34 6.416804999932438 2 35 6.83612891715946 2 36 6.397140897467569 2 37 7.613111814806899 2 38 6.477628426680493 2 39 6.910937148197078 2 40 2.8292400988281905 2 41 8.649405825523527 2 42 7.524400519387516 2 43 6.924352623315258 2 44 -4.4484020400587205 2 45 5.958543859869202 2 46 7.7403017470230004 2 47 2.228589453952644 2 48 6.660955664305671 2 49 0.9467777648138878 2 50 6.3978837577696765 2 51 10.127181517135682 2 52 8.698062922928308 2 53 9.341648053987996 2 54 9.651579532627 2 55 9.47781983783225 2 56 9.229520985351176 3 1 1.3109531026436017 3 2 2.2550373079120263 3 3 2.917110177158378 3 4 2.972339069102638 3 5 3.014578503837704 3 6 3.8788586691902225 3 7 2.387639274014383 3 8 2.487925333566743 3 9 2.7307078880796922 3 10 3.985352195586998 3 11 4.818308964965894 3 12 4.781054728203614 3 13 6.046692817975234 3 14 4.526669878311843 3 15 1.307870487847616 3 16 6.768162836786818 3 17 7.787368714249222 3 18 8.38403371970563 3 19 6.025656344412436 3 20 5.6389646194034775 3 21 7.9567954962140774 3 22 8.326490790132858 3 23 7.761700408571873 3 24 8.296518828891887 3 25 7.809289571906252 3 26 7.03383526880998 3 27 7.474482750884942 3 28 6.305455216248294 3 29 7.025113610781055 3 30 6.9618640893143215 3 31 5.34301111563925 3 32 5.847279179902799 3 33 6.115543541690478 3 34 6.373326739062875 3 35 5.774017115917221 3 36 6.664221642809185 3 37 7.049964816877292 3 38 8.138083913222939 3 39 7.981330522938279 3 40 9.640834301726745 3 41 13.03242859985065 3 42 12.441584784397872 3 43 10.692468565344242 3 44 12.866297752901943 3 45 11.171794377467542 3 46 13.566388703544739 3 47 13.636456948776658 3 48 13.244806595982691 3 49 15.209717723406023 3 50 14.36682785714856 3 51 11.43359973659738 3 52 13.00655153576475 3 53 13.354070414236446 3 54 11.918660277968616 3 55 14.158979258122109 3 56 12.293703179968155 4 1 1.2113860030765053 4 2 1.6576347105094271 4 3 2.0015257615739586 4 4 2.8792654760290404 4 5 1.646613135872336 4 6 3.6948759852075383 4 7 1.7426176289927398 4 8 1.4857608314022386 4 9 2.0813572387290407 4 10 3.3727980830328903 4 11 5.46116610782304 4 12 4.956379403528288 4 13 6.280459051741474 4 14 4.779916631558592 4 15 6.753757933735059 4 16 6.402361970985947 4 17 8.254901181781687 4 18 8.405678741350652 4 19 6.852496171252259 4 20 5.7645057449446035 4 21 5.552033591452172 4 22 7.902248365890429 4 23 7.138323785195251 4 24 7.7402417726148265 4 25 6.982449745066429 4 26 6.603099338074053 4 27 5.381409157811344 4 28 5.710217121010199 4 29 3.973165558833003 4 30 6.745413872864109 4 31 4.1373834100115445 4 32 5.446846279469895 4 33 4.028963455110393 4 34 4.282417648153782 4 35 4.397393739293843 4 36 5.001883980471522 4 37 4.935246202158673 4 38 6.542845817984844 4 39 5.989988531596289 4 40 2.0347736956661358 4 41 9.854939422361472 4 42 8.71214755496064 4 43 8.859135232010907 4 44 7.974522861127049 4 45 7.128504334177499 4 46 10.248206885362919 4 47 9.355071667391378 4 48 9.666884518060609 4 49 13.094999108687404 4 50 10.542152532473235 4 51 8.364335667333307 4 52 8.868023397236609 4 53 10.52939508956112 4 54 9.074504433812773 4 55 12.18928228842514 4 56 10.60106248732746 5 1 -0.6017453100548096 5 2 -0.05448650161178392 5 3 0.7186974787456748 5 4 -0.4641688674052986 5 5 -0.19177070251150496 5 6 1.60901739934895 5 7 0.5304964168715287 5 8 0.5514173970588061 5 9 1.0561047134765218 5 10 2.0091617193965234 5 11 3.072277218934154 5 12 2.6937531409020252 5 13 4.133994405276823 5 14 4.542542894184855 5 15 4.2840609640380904 5 16 4.331654900278881 5 17 6.073082999963507 5 18 7.193557529229441 5 19 3.9939103126664044 5 20 5.289758270197122 5 21 5.829811369229951 5 22 4.993157456799523 5 23 3.6029702498417144 5 24 5.780645813018868 5 25 4.3410356036522835 5 26 4.279867014841727 5 27 1.7046414810436694 5 28 3.099106009899085 5 29 4.326700912368352 5 30 2.1999593274095623 5 31 1.4303127029408387 5 32 2.0377553703789886 5 33 0.04411497026190503 5 34 2.595548961285097 5 35 1.2660806079807152 5 36 1.5372375158250584 5 37 -0.3071780402655655 5 38 1.542845817984844 5 39 1.1162511578589118 5 40 -0.4147212538288123 5 41 5.183222250644302 5 42 3.1717435145565993 5 43 2.5258018986775745 5 44 6.191694578298765 5 45 4.941635647308814 5 46 6.6774998146558495 5 47 9.445980758300468 5 48 7.252743103919197 5 49 9.574797088485386 5 50 8.668415158735858 5 51 2.576456879454522 5 52 3.292265821479031 5 53 1.655657715823743 5 54 0.8472317065400468 5 55 1.9923125914554412 5 56 0.5556079418729141 6 1 1.5093658010562976 6 2 2.389957942832659 6 3 2.9964752565234534 6 4 3.0913866881502514 6 5 3.1415626308218307 6 6 4.220128510460057 6 7 2.5304964168715287 6 8 2.7180840637254704 6 9 2.7783269356987432 6 10 4.286939497174302 6 11 6.183388330045261 6 12 6.915975363124247 6 13 7.411772183054602 6 14 6.098098449740412 6 15 7.22850540848254 6 16 7.442766011389988 6 17 9.01752744440795 6 18 9.47133530700722 6 19 6.32724364599974 6 20 7.511980492419351 6 21 7.829811369229951 6 22 8.215379679021744 6 23 7.880748027619493 6 24 8.613979146352204 6 25 8.39659115920784 6 26 6.8909781259528415 6 27 6.093530369932555 6 28 6.543550454343535 6 29 7.548923134590574 6 30 7.144403771854005 6 31 6.3747571473852815 6 32 6.315533148156767 6 33 6.210781636928573 6 34 5.53999340572954 6 35 5.043858385758494 6 36 6.815015293602837 6 37 6.526155293067767 6 38 7.098401373540401 6 39 7.227362268970026 6 40 7.529723190615631 6 41 9.516555583977631 6 42 10.005076847889935 6 43 10.136913009788685 6 44 11.080583467187658 6 45 8.886080091753257 6 46 12.844166481322517 6 47 12.390425202744911 6 48 12.863854215030312 6 49 11.019241532929833 6 50 11.168415158735858 6 51 11.576456879454518 6 52 11.792265821479035 6 53 12.655657715823743 6 54 7.9583428176511575 6 55 9.825645924788773 6 56 10.500052386317357 7 1 0.670476912167409 7 2 1.2121801650548818 7 3 2.024253034301232 7 4 2.2247200214835843 7 5 1.7693404085996107 7 6 3.73123962157117 7 7 2.24716308353819 7 8 2.490306285947689 7 9 2.294993602365409 7 10 3.909161719396522 7 11 3.811166107823041 7 12 6.638197585346468 7 13 6.9895499608323775 7 14 6.048098449740415 7 15 6.517394297371425 7 16 7.320543789167768 7 17 7.923082999963508 7 18 9.360224195896105 7 19 6.366132534888624 7 20 6.700869381308234 7 21 7.302033591452172 7 22 8.143157456799521 7 23 6.49741469428616 7 24 7.5084235907966494 7 25 8.068813381430065 7 26 6.957644792619504 7 27 6.276863703265892 7 28 6.460217121010199 7 29 6.532256467923908 7 30 6.849959327409561 7 31 6.019201591829727 7 32 6.437755370378987 7 33 5.6107816369285715 7 34 4.623326739062875 7 35 4.888302830202935 7 36 4.042793071380618 7 37 6.4761552930677695 7 38 6.8928458179848455 7 39 6.0718067134144675 7 40 7.1575009683934105 7 41 10.427666695088742 7 42 10.39396573677882 7 43 11.109135232010907 7 44 10.697250133854325 7 45 8.669413425086596 7 46 9.16638870354474 7 47 10.145980758300471 7 48 8.930520881696975 7 49 9.835908199596496 7 50 10.573970714291416 7 51 10.059790212787853 7 52 12.008932488145696 7 53 11.611213271379299 7 54 11.347231706540047 7 55 13.12564592478877 7 56 12.205607941872913 8 1 1.414594559226238 8 2 2.5239448709372354 8 3 2.950723622536529 8 4 2.74824943324829 8 5 2.9193404085996093 8 6 4.3018278568652875 8 7 2.491280730597019 8 8 2.440306285947692 8 9 2.5626406611889365 8 10 3.9209264252788785 8 11 5.725871990175982 8 12 4.932315232405294 8 13 7.366020549067677 8 14 5.627510214446296 8 15 6.940923709136129 8 16 7.191132024461883 8 17 8.308377117610565 8 18 9.683753607660808 8 19 8.157309005476858 8 20 7.04792820483765 8 21 7.228504179687469 8 22 8.699039809740697 8 23 7.635649988403802 8 24 9.234894179031944 8 25 7.971754557900649 8 26 6.792938910266564 8 27 6.54451076208942 8 28 5.151393591598431 8 29 5.411668232629793 8 30 7.25878285682133 8 31 3.7015545330061954 8 32 4.567167135084873 8 33 5.622546342810928 8 34 4.490973797886404 8 35 5.605949889026466 8 36 6.6810283654982605 8 37 5.996743528361883 8 38 7.33696346504367 8 39 7.348277301649766 8 40 2.2486774389816446 8 41 9.068843165676977 8 42 10.746906913249408 8 43 10.712076408481494 8 44 10.070779545619025 8 45 9.101766366263064 8 46 11.801682821191797 8 47 12.857745464182823 8 48 11.648167940520505 8 49 11.803555258420026 8 50 12.047500126056118 8 51 10.909790212787854 8 52 11.017756017557467 8 53 11.093566212555771 8 54 10.935467000657692 8 55 10.472704748318186 8 56 10.232078530108211 9 1 1.7315880232785261 9 2 2.7232912761659946 9 3 3.385364145412346 9 4 2.7024977992613657 9 5 3.308229297488495 9 6 4.10901739934895 9 7 2.75271863909375 9 8 2.8847507303921347 9 9 2.722771380143186 9 10 4.231383941618745 9 11 6.127832774489704 9 12 6.971530918679804 9 13 7.022883294165716 9 14 6.431431783073748 9 15 7.117394297371426 9 16 7.220543789167767 9 17 7.51752744440795 9 18 9.249113084784991 9 19 7.938354757110847 9 20 7.123091603530458 9 21 5.552033591452172 9 22 7.715379679021744 9 23 7.102970249841714 9 24 6.891756924129982 9 25 7.674368936985619 9 26 6.446533681508392 9 27 5.815752592154777 9 28 5.543550454343535 9 29 6.715589801257245 9 30 7.033292660742898 9 31 5.70809048071861 9 32 5.371088703712324 9 33 6.821892748039687 9 34 5.873326739062875 9 35 5.654969496869601 9 36 5.926126404713951 9 37 6.526155293067767 9 38 6.37617915131818 9 39 8.782917824525583 9 40 5.307500968393409 9 41 10.961000028422081 9 42 10.505076847889935 9 43 10.914690787566464 9 44 12.636139022743208 9 45 10.441635647308814 9 46 13.399722036878075 9 47 13.445980758300468 9 48 12.474965326141419 9 49 13.408130421818719 9 50 14.168415158735858 9 51 12.909790212787854 9 52 11.736710265923477 9 53 12.2112132713793 9 54 13.069453928762268 9 55 13.992312591455438 9 56 11.666719052984028 10 1 0.9625821753253092 10 2 1.270074901896983 10 3 1.4584635606170195 10 4 2.3457726530625322 10 5 1.8140772507048695 10 6 2.989134358413274 10 7 0.670847294064508 10 8 0.9139904964740069 10 9 1.8660462339443527 10 10 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-3.826746514426805 114 48 -1.1058427546666607 114 49 -11.314091800403503 114 50 -0.6851201947994952 114 51 0.36433566733330736 114 52 -0.9501584209452112 114 53 -1.5160594558934264 114 54 1.1654135247218633 114 55 -1.4470813479385 114 56 1.9646988509638241 115 1 0.6204769121674119 115 2 1.6121801650548804 115 3 1.6075863676345676 115 4 2.258053354816923 115 5 2.4193404085996093 115 6 2.5812396215711715 115 7 1.697163083538193 115 8 0.27363961928102754 115 9 1.2783269356987432 115 10 -0.15750494727014086 115 11 -0.9555005588436245 115 12 -2.695135747986864 115 13 -7.81045003916762 115 14 -5.151901550259588 115 15 -5.882605702628574 115 16 1.6372104558344311 115 17 3.573082999963507 115 18 1.8602241958961052 115 19 2.38279920155529 115 20 2.567536047974901 115 21 3.885366924785508 115 22 2.909824123466187 115 23 3.7974146942861573 115 24 3.8917569241299823 115 25 4.785480048096726 115 26 3.47431145928617 115 27 4.426863703265891 115 28 3.043550454343535 115 29 4.465589801257245 115 30 6.033292660742898 115 31 2.9858682584963887 115 32 2.454422037045653 115 33 4.127448303595237 115 34 3.956660072396211 115 35 0.7383028302029366 115 36 2.4261264047139512 115 37 2.6094886264011024 115 38 6.292845817984844 115 39 5.255140046747805 115 40 5.307500968393409 115 41 6.211000028422077 115 42 6.060632403445489 115 43 2.692468565344239 115 44 -3.5027498661456775 115 45 5.552746758419925 115 46 -0.35027796312192905 115 47 -6.970685908366198 115 48 1.780520881696976 115 49 -6.730758467070169 115 50 2.307304047624747 115 51 5.659790212787854 115 52 1.6255991548123667 115 53 1.5445466047126324 115 54 2.680565039873379 115 55 2.992312591455441 115 56 6.055607941872914 116 1 0.42816921985971845 116 2 1.1891032419779606 116 3 1.0050222650704654 116 4 1.309335406098974 116 5 0.9193404085996093 116 6 2.138931929263478 116 7 1.2740861604612732 116 8 2.055690901332305 116 9 2.0603782177500207 116 10 2.739930950165757 116 11 3.115012261669193 116 12 3.3305052776541615 116 13 4.8049345762169935 116 14 3.2519446035865656 116 15 -0.8056826257054972 116 16 4.259005327629307 116 17 5.457698384578894 116 18 6.937147272819185 116 19 3.9084402271963192 116 20 4.55471553515439 116 21 6.1289566683752525 116 22 4.070080533722603 116 23 5.739722386593851 116 24 6.75073128310434 116 25 4.695736458353142 116 26 4.634567869542586 116 27 5.196094472496657 116 28 3.2871401979332795 116 29 4.343794929462369 116 30 3.6614977889480222 116 31 5.203816976445111 116 32 4.4992938319174485 116 33 5.287704713851653 116 34 5.681019046755182 116 35 3.10368744558755 116 36 6.785100763688309 116 37 4.756924523837 116 38 4.812076587215611 116 39 8.094883636491389 116 40 9.30750096839341 116 41 9.512282079704132 116 42 7.009350352163434 116 43 2.51298138585706 116 44 6.093403980008169 116 45 4.604028809701976 116 46 1.104850242006279 116 47 -11.246326934007223 116 48 1.1459054970815927 116 49 -5.314091800403503 116 50 0.762432252752955 116 51 3.448251751249394 116 52 3.343547872761082 116 53 5.134290194456224 116 54 2.8087701680785067 116 55 4.90256900171185 116 56 6.363300249565221 117 1 -0.1795230878325853 117 2 0.8121801650548832 117 3 1.4742530343012348 117 4 1.62472002148359 117 5 1.6193404085996121 117 6 2.5312396215711743 117 7 0.8971630835381958 117 8 0.9403062859476918 117 9 1.1449936023654104 117 10 2.2091617193965263 117 11 -3.2388338921769595 117 12 -14.561802414653531 117 13 -18.51045003916762 117 14 -9.101901550259587 117 15 -9.682605702628575 117 16 -11.079456210832234 117 17 1.573082999963507 117 18 -0.6397758041038948 117 19 1.3161325348886272 117 20 -2.6991306186917647 117 21 6.152033591452174 117 22 7.193157456799526 117 23 4.047414694286157 117 24 6.358423590796644 117 25 6.018813381430061 117 26 4.957644792619504 117 27 5.026863703265892 117 28 4.0102171210101965 117 29 3.8822564679239093 117 30 5.899959327409565 117 31 5.019201591829727 117 32 4.13775537037899 117 33 2.8107816369285743 117 34 0.8733267390628754 117 35 2.388302830202935 117 36 3.692793071380617 117 37 4.126155293067768 117 38 4.042845817984844 117 39 5.0718067134144675 117 40 7.507500968393412 117 41 6.727666695088747 117 42 6.793965736778819 117 43 -2.940864767989094 117 44 -2.652749866145676 117 45 5.019413425086594 117 46 1.4663887035447374 117 47 -2.654019241699533 117 48 2.030520881696976 117 49 -4.414091800403504 117 50 1.5239707142914156 117 51 7.0097902127878555 117 52 5.8589324881456974 117 53 5.9112132713792995 117 54 6.847231706540047 117 55 4.325645924788773 117 56 7.755607941872913 118 1 0.12047691216741185 118 2 1.1121801650548804 118 3 1.774253034301232 118 4 1.9247200214835871 118 5 1.9193404085996093 118 6 2.60901739934895 118 7 1.197163083538193 118 8 1.3291951748365776 118 9 1.1116602690320718 118 10 2.6202728305076377 118 11 1.072277218934154 118 12 -5.361802414653532 118 13 -13.366005594723177 118 14 -7.346345994704031 118 15 -10.993716813739685 118 16 -10.501678433054455 118 17 -0.7046947778142716 118 18 -7.473109137437227 118 19 0.549465868221958 118 20 -0.4880195075806526 118 21 5.163144702563287 118 22 5.104268567910637 118 23 5.602970249841714 118 24 6.280645813018868 118 25 6.174368936985619 118 26 5.779867014841727 118 27 5.204641481043669 118 28 3.7657726765657564 118 29 2.5489231345905736 118 30 2.977737105187341 118 31 1.596979369607503 118 32 1.25997759260121 118 33 0.76633719248413 118 34 2.0399934057295397 118 35 2.0438583857584938 118 36 2.75945973804728 118 37 3.4150441819566524 118 38 3.487290262429287 118 39 2.727362268970026 118 40 6.418612079504523 118 41 6.461000028422077 118 42 5.949521292334378 118 43 -3.085309212433536 118 44 1.2472501338543225 118 45 6.886080091753257 118 46 -1.878055740899704 118 47 0.44598075830046824 118 48 -0.2472568960808026 118 49 -0.6474251337368351 118 50 1.223970714291415 118 51 3.909790212787854 118 52 4.625599154812367 118 53 2.322324382490411 118 54 4.4583428176511575 118 55 3.270090369233216 118 56 6.500052386317357 119 1 0.6204769121674119 119 2 1.2788468317215447 119 3 2.274253034301232 119 4 2.3413866881502514 119 5 2.4193404085996093 119 6 2.747906288237836 119 7 1.7804964168715287 119 8 2.0236396192810275 119 9 2.0283269356987432 119 10 3.592495052729859 119 11 -2.205500558843628 119 12 -9.611802414653532 119 13 -2.7271167058342876 119 14 -7.73523488359292 119 15 -12.382605702628574 119 16 -12.362789544165565 119 17 -0.926917000036493 119 18 -5.056442470770563 119 19 -1.2838674651113742 119 20 -2.0991306186917633 119 21 4.468700258118837 119 22 4.743157456799523 119 23 3.9640813609528216 119 24 5.308423590796647 119 25 5.368813381430062 119 26 6.057644792619506 119 27 6.0101970365992265 119 28 6.043550454343535 119 29 6.382256467923909 119 30 6.699959327409562 119 31 5.40253492516306 119 32 5.204422037045653 119 33 6.460781636928573 119 34 5.123326739062875 119 35 6.321636163536272 119 36 7.3427930713806155 119 37 7.359488626401102 119 38 8.12617915131818 119 39 8.088473380081133 119 40 10.224167635060073 119 41 8.627666695088745 119 42 8.060632403445485 119 43 -1.8908647679890933 119 44 -2.5860831994790097 119 45 9.136080091753257 119 46 0.3163887035447388 119 47 -3.8040192416995318 119 48 -4.469479118303024 119 49 -0.6474251337368351 119 50 3.223970714291415 119 51 7.826456879454522 119 52 8.292265821479031 119 53 8.9612132713793 119 54 7.513898373206715 119 55 9.242312591455441 119 56 9.638941275206246 120 1 -0.21285642116592385 120 2 0.6677357206104375 120 3 1.6631419231901177 120 4 1.0358311325947014 120 5 1.808229297488495 120 6 2.720128510460057 120 7 0.7527186390937501 120 8 0.995861841503249 120 9 0.8894380468098504 120 10 2.5091617193965234 120 11 -4.261056114399182 120 12 -7.917357970209089 120 13 -14.143783372500952 120 14 -9.457457105815145 120 15 -3.6603834804063524 120 16 -16.612789544165565 120 17 -1.3713614444809359 120 18 -9.139775804103895 120 19 -6.450534131778042 120 20 -4.043575063136206 120 21 2.1631447025632866 120 22 2.659824123466187 120 23 2.2696369165083787 120 24 3.9473124796855323 120 25 3.2854800480967263 120 26 5.002089237063949 120 27 4.649085925488112 120 28 3.987994898787978 120 29 5.660034245701688 120 30 6.199959327409562 120 31 4.3747571473852815 120 32 2.9266442592678743 120 33 4.321892748039687 120 34 5.262215627951761 120 35 4.599413941314051 120 36 6.0927930713806155 120 37 5.303933070845545 120 38 6.820623595762623 120 39 7.282917824525583 120 40 9.974167635060073 120 41 -4.983444416022365 120 42 -0.8282564854434007 120 43 -5.19642032354465 120 44 -16.086083199479013 120 45 -4.22503101935785 120 46 -10.878055740899706 120 47 -5.554019241699532 120 48 -4.802812451636356 120 49 -15.647425133736835 120 50 -3.3315848412641422 120 51 0.46534576834341124 120 52 0.7367102659234774 120 53 1.4334354936015217 120 54 1.236120595428936 120 55 1.1589792581221054 120 56 7.2778301640951355 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 67.0 5 8.0 6 82.0 7 42.0 8 82.0 9 107.0 10 47.0 11 60.0 12 56.0 13 30.0 14 42.0 15 50.0 16 36.0 17 63.0 18 76.0 19 89.0 20 122.0 21 175.0 22 220.0 23 270.0 24 323.0 25 362.0 26 402.0 27 440.0 28 457.0 29 445.0 30 554.0 31 607.0 32 657.0 33 721.0 34 798.0 35 870.0 36 923.0 37 1047.0 38 1176.0 39 1292.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.39839049925774 0.31252441596999764 0.25001953277599814 0.039065551996249705 8 99.1093054144855 0.25001953277599814 0.6172357215407454 0.023439331197749824 9 99.29676511954993 0.2891076730739178 0.26566651039224876 0.14846069698390374 10 70.88835065239472 16.243456520040628 6.008281897023205 6.859910930541449 11 43.37838893663567 25.986405187905305 17.24353465114462 13.3916712243144 12 34.807406828658486 16.07156809125713 22.001718884287836 27.119306195796543 13 28.10375810610204 16.21220407844363 24.376904445659818 31.307133369794514 14 25.072271271193063 19.228064692554106 28.947574029221034 26.752090007031796 15 25.78468298807282 19.444444444444446 26.914626490897675 27.856246076585062 16 35.28216351414726 19.970298577458184 23.448491480381428 21.29904642801313 17 32.611584460251706 20.70663644180411 26.741186586414443 19.940592511529744 18 29.26009844519103 20.556293460426595 28.283459645284786 21.900148449097586 19 33.04932384897991 23.786445712499024 20.487766747440006 22.67646369108106 20 29.887394432280264 25.609946825148576 23.099781044729433 21.402877697841728 21 29.78357684194078 22.400187514649584 25.970778967106806 21.845456676302835 22 25.65044144073756 20.454723025236348 26.142667395890307 27.75216813813579 23 25.38479568716306 24.15032424408157 24.29096023126807 26.173919837487304 24 28.69979684325676 20.932958274730424 25.83216127519925 24.535083606813565 25 28.319137297804176 22.880362584980855 24.130655622411503 24.66984449480347 26 25.62539086929331 22.381175734834272 23.022201375859915 28.971232020012504 27 23.868344930028925 20.944414041122663 24.431240716128528 30.756000312719884 28 24.501914212047815 24.322212672865067 23.83779982811157 27.338073286975543 29 31.21283255086072 23.80281690140845 20.837245696400625 24.147104851330205 30 29.932770481550968 20.58317698561601 25.89118198874296 23.592870544090054 31 29.14290178920228 23.173685444175327 23.204937885772324 24.478474880850065 32 34.03174106793839 21.77312172621374 21.82002970838871 22.37510749745915 33 30.41608008759581 20.147035820428595 23.564836539965587 25.872047552010013 34 25.80417938483212 24.841512092040386 26.586835720435158 22.76747280269234 35 27.377318619394224 24.69280738827581 29.27134695155357 18.658527040776395 36 25.742651657285805 29.541901188242655 22.224828017510944 22.4906191369606 37 27.832550860719873 25.31298904538341 24.890453834115807 21.96400625978091 38 30.84814785809382 25.851672018169005 21.622679927950507 21.67750019578667 39 27.02512326837286 25.311105893402207 20.779525710260625 26.884245127964313 40 23.97826600519726 19.13536498936924 22.537207654145995 34.349161351287506 41 20.143657747257084 17.238929670850105 20.372562948930458 42.24484963296235 42 26.51782216999608 23.392087739913826 24.198981590285936 25.89110849980415 43 23.0371415138693 18.03792509011127 26.46920545369064 32.455727942328785 44 24.042987135236903 22.160338876686538 23.219328522121117 30.577345465955446 45 26.268399624177892 23.152207954901346 26.628562480425934 23.950829940494835 46 24.22817740166118 25.082275505406677 23.460272684532203 27.229274408399938 47 20.705087939698494 22.652324120603016 25.211997487437188 31.43059045226131 48 25.01563477173233 22.521888680425267 28.392745465916196 24.069731081926204 49 24.00908663637788 21.267429108569637 26.594078019739936 28.129406235312548 50 26.575342465753426 21.36986301369863 27.545988258317028 24.50880626223092 51 24.087133678106877 22.32408713367811 28.78859112991694 24.800188058298072 52 24.698606544543605 22.27963049945201 28.777203695005483 24.244559260998905 53 23.8703109092333 20.22084736471141 29.57945023102827 26.329391495027014 54 24.54780361757106 23.42024900164435 28.69783102341242 23.334116357372174 55 23.3672670321065 23.931088488645262 26.734534064213 25.96711041503524 56 25.918095685537544 22.926943857176415 26.614987080103358 24.53997337718268 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 2.0 12 3.0 13 8.0 14 12.0 15 13.5 16 15.0 17 9.5 18 4.0 19 5.0 20 6.0 21 9.5 22 12.0 23 11.0 24 13.5 25 16.0 26 18.5 27 21.0 28 35.0 29 56.5 30 64.0 31 77.5 32 91.0 33 104.0 34 117.0 35 136.0 36 155.0 37 154.0 38 182.0 39 211.0 40 231.5 41 252.0 42 270.5 43 289.0 44 363.0 45 437.0 46 560.0 47 688.5 48 694.0 49 645.5 50 597.0 51 620.5 52 644.0 53 714.5 54 826.0 55 867.0 56 877.5 57 888.0 58 932.0 59 976.0 60 1024.5 61 1073.0 62 1052.0 63 885.5 64 740.0 65 687.5 66 635.0 67 508.5 68 382.0 69 333.5 70 285.0 71 256.0 72 201.0 73 175.0 74 136.0 75 97.0 76 65.5 77 34.0 78 30.0 79 25.0 80 24.0 81 17.5 82 11.0 83 6.5 84 2.0 85 1.0 86 0.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.007813110399249942 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.42972107195874676 16 0.039065551996249705 17 0.04687866239549965 18 0.0 19 0.04687866239549965 20 0.08594421439174936 21 0.0 22 0.0 23 0.0 24 0.007813110399249942 25 0.015626220798499884 26 0.054691772794749585 27 0.06250488319399954 28 0.0 29 0.14844909758574887 30 0.054691772794749585 31 0.0 32 0.06250488319399954 33 0.10157043519024925 34 0.17188842878349872 35 0.17188842878349872 36 0.054691772794749585 37 0.14844909758574887 38 0.23439331197749824 39 0.17188842878349872 40 0.7813110399249942 41 1.0157043519024922 42 0.265645753574498 43 0.28908508477224787 44 0.398468630361747 45 0.2109539807797484 46 0.28908508477224787 47 0.49222595515274636 48 0.054691772794749585 49 0.25783264317524807 50 0.1875146495819986 51 0.28908508477224787 52 0.19532775998124854 53 0.23439331197749824 54 0.21876709117899834 55 0.2265802015782483 56 0.21876709117899834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 12799.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.41229783576841 #Duplication Level Percentage of deduplicated Percentage of total 1 87.99959628582963 68.12250957106023 2 7.216390795316915 11.172747870927417 3 2.149777957206298 4.992577545120713 4 0.9386354461041583 2.906477068520978 5 0.4642712959224869 1.7970153918274865 6 0.3835284618490109 1.7813891710289866 7 0.12111425111021397 0.6563012735369951 8 0.20185708518368994 1.2500976638799906 9 0.10092854259184497 0.7031799359324947 >10 0.4138070246265644 5.516055941870459 >50 0.0 0.0 >100 0.010092854259184497 1.1016485662942417 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTGTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 141 1.1016485662942417 No Hit CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 45 0.35158996796624736 No Hit CTGTGTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 44 0.34377685756699744 No Hit CTGTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 42 0.32815063676849754 No Hit CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 30 0.23439331197749824 No Hit CTGTGTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 28 0.21876709117899834 No Hit CTGTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG 28 0.21876709117899834 No Hit CTGTGTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT 25 0.19532775998124854 No Hit CTGTGTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 24 0.1875146495819986 No Hit CTGTGTGGGATGGCTCGTGTGCAGCGTAGATCTATCACATGTGGAAGTGTGTATAC 24 0.1875146495819986 No Hit CTGTGTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 23 0.17970153918274867 No Hit CTGTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 22 0.17188842878349872 No Hit CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 18 0.14063598718649895 No Hit CTGTGTGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT 17 0.132822876787249 No Hit CTGTGTGGGGACTTTGTTCTCTTAAGAAAACCCTGGAATTGTTCTGTTTCCCCAGC 16 0.12500976638799907 No Hit CTGTGTGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 16 0.12500976638799907 No Hit CTGTGTGGGGGAGACATGATCCTTTTGTGTCTGGCAGGCCTCATGCAACAAATTTT 16 0.12500976638799907 No Hit CTGTGTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 15 0.11719665598874912 No Hit CTGTGTGGGAGGGCGTGGGAGCTGTGGTCACGGCATGCATCTCAGTGAAAGAAGAT 13 0.10157043519024925 No Hit CTGTGTGGGGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA 13 0.10157043519024925 No Hit CTGTGTGGGGGGACTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC 13 0.10157043519024925 No Hit CTGTGTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAGGAGCACTGACTGCTCTTCG 13 0.10157043519024925 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.007813110399249942 0.0 0.0 0.0 0.0 23 0.007813110399249942 0.0 0.0 0.0 0.0 24 0.007813110399249942 0.0 0.0 0.0 0.0 25 0.007813110399249942 0.0 0.0 0.0 0.0 26 0.007813110399249942 0.0 0.0 0.0 0.0 27 0.007813110399249942 0.0 0.0 0.0 0.0 28 0.007813110399249942 0.0 0.0 0.0 0.0 29 0.007813110399249942 0.0 0.0 0.0 0.0 30 0.007813110399249942 0.0 0.0 0.0 0.0 31 0.007813110399249942 0.0 0.0 0.0 0.0 32 0.015626220798499884 0.0 0.0 0.0 0.0 33 0.015626220798499884 0.0 0.0 0.0 0.0 34 0.015626220798499884 0.0 0.0 0.0 0.0 35 0.015626220798499884 0.0 0.0 0.0 0.0 36 0.015626220798499884 0.0 0.0 0.0 0.0 37 0.015626220798499884 0.0 0.0 0.0 0.0 38 0.023439331197749824 0.0 0.0 0.0 0.0 39 0.023439331197749824 0.0 0.0 0.0 0.0 40 0.023439331197749824 0.0 0.0 0.0 0.0 41 0.023439331197749824 0.0 0.0 0.0 0.0 42 0.023439331197749824 0.0 0.0 0.0 0.0 43 0.023439331197749824 0.0 0.0 0.0 0.0 44 0.023439331197749824 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACATGT 15 0.008134665 50.165356 36 ACATGTG 15 0.008134665 50.165356 37 TATGCTC 15 0.008134665 50.165356 35 CCTGCCG 15 0.008134665 50.165356 40 ATGTGGA 15 0.008134665 50.165356 39 TGCTCGT 15 0.008134665 50.165356 37 ATGCTCG 15 0.008134665 50.165356 36 TCGTCTC 15 0.008134665 50.165356 40 CGTGCAT 15 0.008134665 50.165356 41 ATCACAT 15 0.008134665 50.165356 34 TGTGGAA 15 0.008134665 50.165356 40 CTGCCGT 15 0.008134665 50.165356 41 TCACATG 15 0.008134665 50.165356 35 CATGTGG 15 0.008134665 50.165356 38 GTTGATC 45 1.1641532E-10 49.96863 16 GTACCTG 45 1.1641532E-10 49.96863 9 ACCTGGT 45 1.1641532E-10 49.96863 11 TGGGGTG 45 1.1641532E-10 49.96863 6 GTGGGAT 75 0.0 49.96863 5 TTGATCC 45 1.1641532E-10 49.96863 17 >>END_MODULE