FastQCFastQC Report
Fri 17 Jun 2016
SRR1526037_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526037_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4314
Sequences flagged as poor quality0
Sequence length40
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG190.440426518312471No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGGGGGGCTGGTGA110.25498377375985165No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG90.2086230876216968No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC90.2086230876216968No Hit
CTGTGTGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC80.18544274455261936No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG70.16226240148354196No Hit
CTGTGTGGGCTCGTCTTACTCAGCAACATCTTTTGATACA60.13908205841446453No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC60.13908205841446453No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAG50.11590171534538712No Hit
CTGTGTGGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGA50.11590171534538712No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC50.11590171534538712No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG50.11590171534538712No Hit
CTGTGTGGGGTCTCTTTTCCTCTGGCGCGGTGAGTGAGTT50.11590171534538712No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGCT301.1702594E-534.0000046
GTGGGGC453.5797711E-934.05
GTGGGCA251.7128444E-434.05
TGGAGAG200.002492687733.99999615
TGGGGAG405.3541953E-833.9999966
GGGGCTC200.002492687733.9999967
GGGCTCT200.002492687733.9999968
GTGGGGA604.3655746E-1131.1666685
TGTGGGT556.6029315E-1030.909094
TGTGGGC451.5092519E-730.2222214
TGTGGGG2750.029.6727284
GTGTGGG4900.029.4897943
CTGTGTG5000.029.241
TGTGTGG5000.029.242
GTGGGTG353.3623888E-529.1428595
GTGGGAG353.3623888E-529.1428595
GTGGGAC353.3623888E-529.1428595
GAGTGCA304.9961044E-428.33333610
AGTGCAG304.9961044E-428.33333611
GTGGGGG1250.027.25
Produced by FastQC (version 0.11.5)