FastQCFastQC Report
Fri 17 Jun 2016
SRR1526036_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526036_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022
Sequences flagged as poor quality0
Sequence length40
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT40.3913894324853229No Hit
CTGTGTGGGGAGTCGCCCTTTTTTTTTTTTTTTTTTTTTT30.29354207436399216No Hit
CTGTGTGGGTGTCCGCCTTTTTTTTTTTTTTTTTTTTTTT30.29354207436399216No Hit
CTGTGTGGGTCGTCTTTTTTTTTTTTTCCTTTTTTTTTTT30.29354207436399216No Hit
CTGTGTGGGGTGGTGTGTGTGTGTCAGTGTGATGTGTGTG30.29354207436399216No Hit
CTGTGTGGGCAATTCGCTTTTTTTTTTTTTTTTTTTTTTT20.19569471624266144No Hit
CTGTGTGGGTGTCCGACTTTTTTTTTTTTTTTTTTTTTTT20.19569471624266144No Hit
CTGTGTGGGCGTATCCGCTCGTTTTTTTTTTTTTTTTTTT20.19569471624266144No Hit
CTGTGTGGGTCGTCTTTTTTTTTTTTTTCTTTTTTTTTTT20.19569471624266144No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCCGTGGTAGCCCC20.19569471624266144No Hit
CTGTGTGGGAGAGTCGATTTTTTTTTTTTTTTTTTTTTTT20.19569471624266144No Hit
CTGTGTGGGGGGCTGGTGCGATGGCCCAGTGGGTAAGAGC20.19569471624266144No Hit
CTGTGTGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT20.19569471624266144No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCCGTGGTCGCGCC20.19569471624266144No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTCGCGCC20.19569471624266144No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGGGA200.001662030434.05
CTGTGTG1250.027.1999991
TGTGGGA250.00497942727.1999994
GTGTGGG1250.027.1999993
TGTGTGG1250.027.1999992
TGTGGGG701.0913936E-1126.7142854
Produced by FastQC (version 0.11.5)