FastQCFastQC Report
Fri 17 Jun 2016
SRR1526035_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526035_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431
Sequences flagged as poor quality0
Sequence length40
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT181.257861635220126No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC171.187980433263452No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG130.9084556254367574No Hit
CTGTGTGGGGGGGAGGTAGTGACGAAAAATAACAATACAG50.3494060097833683No Hit
CTGTGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC50.3494060097833683No Hit
CTGTGTGGGGGTGTAGCTCAGTTGGTAAGTAGTTGCCTAG40.2795248078266946No Hit
CTGTGTGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAG40.2795248078266946No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC40.2795248078266946No Hit
CTGTGTGGGGTTTGATCGTACTAAAAACTCTTAAGAAAAA40.2795248078266946No Hit
CTGTGTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAG40.2795248078266946No Hit
CTGTGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT30.20964360587002098No Hit
CTGTGTGGGATCACCATGAAGGTCGAGCTGTGCAGTTTCA30.20964360587002098No Hit
CTGTGTGGGGACATTAAGCCAACTCCAGGATTCGGGAGTT30.20964360587002098No Hit
CTGTGTGGGATTGTATTGTCTCACACATGCTCTATATAAA30.20964360587002098No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG30.20964360587002098No Hit
CTGTGTGGGGTTTTCATATTGATAAAATTATTATTGCTTC30.20964360587002098No Hit
CTGTGTGGGGCTGTCAGTTGTTTTATTGATCTCAGCCACT30.20964360587002098No Hit
CTGTGTGGGGACTCTGATTCCGACACATGACTCCGAGGTG30.20964360587002098No Hit
CTGTGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGG30.20964360587002098No Hit
CTGTGTGGGCTGTTAGTAGGATTCTTACGTTTGCCTTTCG30.20964360587002098No Hit
CTGTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCG30.20964360587002098No Hit
CTGTGTGGGGGAGAGGGACTCTTCAGTATGGAGGCAGCCT30.20964360587002098No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAG20.1397624039133473No Hit
CTGTGTGGGGGTTTGGGGCTAGGTTGGTAGGAAGTTTACC20.1397624039133473No Hit
CTGTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1397624039133473No Hit
CTGTGTGGGGGGGTGGGGATAACCTGTGAGTGCAGCCGGG20.1397624039133473No Hit
CTGTGTGGGGGACATGATAGCAGTTACCTGCATTCCTGTC20.1397624039133473No Hit
CTGTGTGGGGAGAGTGAGTTCCAGGACAGCCAGGGCTACA20.1397624039133473No Hit
CTGTGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCT20.1397624039133473No Hit
CTGTGTGGAGGAGGTTTTGATTTATTTCCTCTTCTTGCTC20.1397624039133473No Hit
CTGTGTGGGGCTTTCCGAGCTGCTAGCGCTCCTGCGAACA20.1397624039133473No Hit
CTGTGTGGGAGTCCTCATTAAGACACAAAGGCCTGGGTGG20.1397624039133473No Hit
CTGTGTGGGGGGCTGGAGAGATGGCTCAGCAGTTATTAGC20.1397624039133473No Hit
CTGTGTGGGGAGTCGAGCCCTATCTCAAGTCACTAATGCT20.1397624039133473No Hit
CTGTGTGGGCGGGGAGGTAGTGACGAAAAATAACAATACA20.1397624039133473No Hit
CTGTGTGGGCCTAATCCTAGCCCTAGCCCTACACAAATAT20.1397624039133473No Hit
CTGTGTGATTGTATATGTTTGTGTAAATGTGTGTGTAAAT20.1397624039133473No Hit
CTGTGTGGGGGGAAGGGGTGGATTAGAAACAAAAATTCTG20.1397624039133473No Hit
CTGTGTGGGGGGCTCTGTTTGCTACAGAAAAGAGTGAAAC20.1397624039133473No Hit
CTGTGTGGGGGTCGTGGTGTGCCCAGCTCTTCCAAGGACT20.1397624039133473No Hit
CTGTGTGGGGACTGCTGGAGGAAGATTGCAAAAGATGAAG20.1397624039133473No Hit
CTGTGTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAAAGC20.1397624039133473No Hit
CTGTGTGGGGTAGTGGTTGGTGTTGGCAGAGCTGGCTCTG20.1397624039133473No Hit
CTGTGTGGGGACTGGGGTTCTCACAGGGAATCCCCCTTGC20.1397624039133473No Hit
CTGTGTGGGGGCTGGCTAGATGGCTCAGCGGATAAGAGCA20.1397624039133473No Hit
CTGTGTGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT20.1397624039133473No Hit
CTGTGTGGGGGTCCATAGTGGGTTCAGGGATGAAGCTTGC20.1397624039133473No Hit
CTGTGTGGGGTGGCCATCTTCCACAAGTACTCTGGCAAGG20.1397624039133473No Hit
CTGTGTGGGGGACGAGAGGCTTTGTCCGGTTCGGCGGTCT20.1397624039133473No Hit
CTGTGTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC20.1397624039133473No Hit
CTGTGTGGGGGGCTGGAGAGATGGCTCAGCGGTTGAGAGC20.1397624039133473No Hit
CTGTGTGGGATACATTTAAGATTTTTTTTAAGGTAACAGA20.1397624039133473No Hit
CTGTGTGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAGC20.1397624039133473No Hit
CTGTGTGGGTGTGTTCTATATTCCAAATTAACTAGGCCTA20.1397624039133473No Hit
CTGTGTGGGGAATGTTTGAAGTTCTCAGTTATTGTTTACT20.1397624039133473No Hit
CTGTGTGGGGAGAAGGTAAAAACTCTAATTTTCTATCAAA20.1397624039133473No Hit
CTGTGTGGGATGGCTCGTATGCCGTCTTCTGCTTGGATCG20.1397624039133473Illumina Single End Adapter 2 (95% over 24bp)
CTGTGTGGGGACCCTGAGGGACCTCTAGAGCATTTCTGAG20.1397624039133473No Hit
CTGTGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGC20.1397624039133473No Hit
CTGTGTGGGATTGTATGTGGCCTGGACTCTATCATAGCGA20.1397624039133473No Hit
CTGTGTGGGGGCTGGGACTCCTACTCCACCCTAGCAGTTC20.1397624039133473No Hit
CTGTGTGGGGGGACTGGAGAGATGGCTCAGCGGTTAAGAG20.1397624039133473No Hit
CTGTGTGGGGACATCACTAATGAGGAATATGGAGAGTTCT20.1397624039133473No Hit
CTGTGTTTGTGGGAGATGGTTCTTTTTTTGATTCTAATAA20.1397624039133473No Hit
CTGTGTGGGGAAGGTAAGCATCATTGTACCTTTAATATGT20.1397624039133473No Hit
CTGTGTGGGGGAAACATTCTATTAATGGTGGCCCGTCTCA20.1397624039133473No Hit
CTGTGTGGGGGCTACCAAATCCAAGGAAGGCAGCAGGCGC20.1397624039133473No Hit
CTGTGTGGGAACCAGGAGCTGCAAGAGATCAACAGAGTGT20.1397624039133473No Hit
CTGTGTGGGGGGTGCCTGGGTGATCCGCCTTGTGGATCGA20.1397624039133473No Hit
CTGTGTGGGGGCTAGACGTACAGCGACTCTGTGTCATTTA20.1397624039133473No Hit
CTGTGTGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTC20.1397624039133473No Hit
CTGTGTGGGGGAGCACCTTTTTACTTTGGTAGGTATGCAG20.1397624039133473No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTG20.1397624039133473No Hit
CTGTGTGGGATGTTTTATAAATAAAATGTTTTTCCTCAAA20.1397624039133473No Hit
CTGTGTGGGGGATTTCTGAGTTCGAGGCCAGCCTGGTCTA20.1397624039133473No Hit
CTGTGTGGGATTTAAGGAGAATTGTGTAAGATAAGCTTGT20.1397624039133473No Hit
CTGTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA20.1397624039133473No Hit
CTGTGTGGGATTTTGGATGATTAGAAGCACAGAATGATCA20.1397624039133473No Hit
CTGTGTGGGGACTTTGTTCTCTTAAGAAAACCCTGGAATT20.1397624039133473No Hit
CTGTGTGGGACTAATCCTAGCCCTAGCCCTACACAAATAT20.1397624039133473No Hit
CTGTGTGGGGGTTTTCAGTCACAACACTTTCACTGTTTGT20.1397624039133473No Hit
CTGTGTGAATGTGTGTGCATGTGTATGGGGGATGTAAAAC20.1397624039133473No Hit
CTGTGTGGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAG20.1397624039133473No Hit
CTGTGTGGGGTATTAGTGGGGGTTGATTTGTGTGTTGGTT20.1397624039133473No Hit
CTGTGTGGGGGGTAACAATATAGATAAAATCCCCATGGAG20.1397624039133473No Hit
CTGTGTGGGGGAGGAGGAGTGAGAGCGGCAAAATGGCGGA20.1397624039133473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGTG1400.034.01
TGTGGGG900.034.04
GTGTGGG1400.034.03
TGTGTGG1400.034.02
GTGGGGG402.0472726E-833.9999965
GTGGGGC200.001945374933.9999965
GTGGGGA200.001945374933.9999965
TGTGGGC200.001945374933.9999964
TGTGGGA200.001945374933.9999964
Produced by FastQC (version 0.11.5)