##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526035_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1431 Sequences flagged as poor quality 0 Sequence length 40 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.01956673654787 33.0 30.0 35.0 4.0 37.0 2 19.690426275331937 22.0 4.0 32.0 4.0 35.0 3 19.559748427672957 22.0 4.0 31.0 4.0 35.0 4 19.033542976939202 21.0 4.0 31.0 4.0 35.0 5 18.48357791754018 19.0 4.0 31.0 4.0 35.0 6 15.798043326345214 10.0 4.0 29.0 4.0 34.0 7 14.814116002795249 4.0 4.0 28.0 4.0 33.0 8 14.0125786163522 4.0 4.0 28.0 4.0 33.0 9 13.133473095737246 4.0 4.0 25.0 4.0 33.0 10 11.069881201956674 4.0 4.0 20.0 4.0 30.0 11 10.414395527603075 4.0 4.0 17.0 4.0 28.0 12 10.637316561844864 4.0 4.0 18.0 4.0 29.0 13 10.343116701607268 4.0 4.0 16.0 4.0 29.0 14 10.474493361285814 4.0 4.0 17.0 4.0 30.0 15 10.119496855345911 4.0 4.0 14.0 4.0 31.0 16 8.37246680642907 4.0 4.0 4.0 4.0 25.0 17 7.96785464709993 4.0 4.0 4.0 4.0 23.0 18 7.925925925925926 4.0 4.0 4.0 4.0 23.0 19 7.540181691125087 4.0 4.0 4.0 4.0 21.0 20 7.483577917540182 4.0 4.0 4.0 4.0 20.0 21 7.59329140461216 4.0 4.0 4.0 4.0 22.0 22 7.385744234800838 4.0 4.0 4.0 4.0 21.0 23 7.69392033542977 4.0 4.0 4.0 4.0 23.0 24 7.580712788259958 4.0 4.0 4.0 4.0 22.0 25 7.755415793151642 4.0 4.0 4.0 4.0 24.0 26 7.87351502445842 4.0 4.0 4.0 4.0 25.0 27 7.782669461914745 4.0 4.0 4.0 4.0 24.0 28 8.037037037037036 4.0 4.0 4.0 4.0 25.0 29 7.311670160726765 4.0 4.0 4.0 4.0 20.0 30 7.8574423480083855 4.0 4.0 4.0 4.0 26.0 31 7.821104122990915 4.0 4.0 4.0 4.0 27.0 32 7.6750524109014675 4.0 4.0 4.0 4.0 24.0 33 7.63591893780573 4.0 4.0 4.0 4.0 24.0 34 7.778476589797345 4.0 4.0 4.0 4.0 28.0 35 7.543675751222921 4.0 4.0 4.0 4.0 25.0 36 7.57162823200559 4.0 4.0 4.0 4.0 27.0 37 7.4926624737945495 4.0 4.0 4.0 4.0 27.0 38 7.320754716981132 4.0 4.0 4.0 4.0 25.0 39 7.264849755415793 4.0 4.0 4.0 4.0 26.0 40 7.076869322152341 4.0 4.0 4.0 4.0 23.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 10.200320512820511 2 2 10.834935897435898 2 3 15.639423076923077 2 4 13.39423076923077 2 5 19.036858974358974 2 6 -12.041666666666668 2 7 -10.681089743589743 2 8 -10.528846153846153 2 9 -9.538461538461538 2 10 -6.8157051282051295 2 11 -6.865384615384615 2 12 -6.5400641025641 2 13 -6.9375 2 14 -7.076923076923077 2 15 -7.153846153846153 2 16 -5.108974358974358 2 17 -4.4935897435897445 2 18 -4.834935897435896 2 19 -4.443910256410257 2 20 -4.459935897435898 2 21 -3.7532051282051277 2 22 -3.259615384615385 2 23 -4.094551282051281 2 24 -3.668269230769231 2 25 -4.112179487179487 2 26 -3.3381410256410255 2 27 -3.5464743589743595 2 28 -3.653846153846154 2 29 -2.7628205128205128 2 30 -3.4246794871794872 2 31 -3.394230769230769 2 32 -2.967948717948717 2 33 -2.221153846153846 2 34 -2.8589743589743595 2 35 -2.447115384615385 2 36 -2.5689102564102564 2 37 -2.782051282051281 2 38 -2.354166666666667 2 39 -2.30128205128205 2 40 -1.9679487179487172 3 1 6.700320512820511 3 2 3.834935897435898 3 3 8.639423076923077 3 4 3.39423076923077 3 5 5.0368589743589745 3 6 2.958333333333332 3 7 6.318910256410257 3 8 11.471153846153847 3 9 0.4615384615384617 3 10 -5.8157051282051295 3 11 3.134615384615385 3 12 3.4599358974358996 3 13 -0.4375 3 14 -0.07692307692307665 3 15 -1.6538461538461533 3 16 3.8910256410256423 3 17 6.0064102564102555 3 18 -1.834935897435896 3 19 -3.4439102564102573 3 20 7.040064102564102 3 21 3.2467948717948723 3 22 8.240384615384615 3 23 5.905448717948719 3 24 4.831730769230769 3 25 2.3878205128205128 3 26 7.6618589743589745 3 27 3.9535256410256405 3 28 6.346153846153846 3 29 -0.7628205128205128 3 30 7.075320512820513 3 31 9.60576923076923 3 32 6.532051282051283 3 33 12.278846153846153 3 34 11.64102564102564 3 35 10.552884615384615 3 36 11.931089743589745 3 37 11.217948717948719 3 38 13.645833333333332 3 39 10.698717948717949 3 40 8.532051282051283 4 1 4.866987179487179 4 2 8.66826923076923 4 3 2.8060897435897445 4 4 1.89423076923077 4 5 9.703525641025642 4 6 -4.708333333333334 4 7 12.65224358974359 4 8 8.471153846153847 4 9 10.461538461538462 4 10 8.850961538461538 4 11 18.134615384615387 4 12 14.793269230769232 4 13 18.0625 4 14 14.589743589743591 4 15 18.846153846153847 4 16 18.224358974358974 4 17 17.173076923076923 4 18 11.498397435897436 4 19 17.22275641025641 4 20 21.20673076923077 4 21 12.580128205128204 4 22 11.407051282051283 4 23 16.23878205128205 4 24 15.33173076923077 4 25 12.55448717948718 4 26 19.995192307692307 4 27 10.45352564102564 4 28 6.67948717948718 4 29 17.903846153846153 4 30 20.908653846153847 4 31 27.272435897435894 4 32 19.365384615384613 4 33 24.778846153846153 4 34 25.474358974358978 4 35 18.88621794871795 4 36 29.431089743589745 4 37 28.884615384615383 4 38 16.645833333333332 4 39 29.365384615384613 4 40 30.032051282051285 5 1 -22.79967948717949 5 2 -14.665064102564102 5 3 -14.860576923076923 5 4 -14.10576923076923 5 5 -13.963141025641026 5 6 -12.041666666666668 5 7 -10.681089743589743 5 8 -10.528846153846153 5 9 -9.538461538461538 5 10 -6.8157051282051295 5 11 -6.865384615384615 5 12 -6.5400641025641 5 13 -6.9375 5 14 -7.076923076923077 5 15 -7.153846153846153 5 16 -5.108974358974358 5 17 -4.4935897435897445 5 18 -4.834935897435896 5 19 -4.443910256410257 5 20 -4.459935897435898 5 21 -3.7532051282051277 5 22 -3.259615384615385 5 23 -4.094551282051281 5 24 -3.668269230769231 5 25 -4.112179487179487 5 26 -3.3381410256410255 5 27 -3.5464743589743595 5 28 -3.653846153846154 5 29 -2.7628205128205128 5 30 -3.4246794871794872 5 31 -3.394230769230769 5 32 -2.967948717948717 5 33 -2.221153846153846 5 34 -2.8589743589743595 5 35 -2.447115384615385 5 36 -2.5689102564102564 5 37 -2.782051282051281 5 38 -2.354166666666667 5 39 -2.30128205128205 5 40 -1.9679487179487172 6 1 -22.79967948717949 6 2 -14.665064102564102 6 3 -14.860576923076923 6 4 -14.10576923076923 6 5 -13.963141025641026 6 6 -12.041666666666668 6 7 -10.681089743589743 6 8 -10.528846153846153 6 9 -9.538461538461538 6 10 -6.8157051282051295 6 11 -6.865384615384615 6 12 -6.5400641025641 6 13 -6.9375 6 14 -7.076923076923077 6 15 -7.153846153846153 6 16 -5.108974358974358 6 17 -4.4935897435897445 6 18 -4.834935897435896 6 19 -4.443910256410257 6 20 -4.459935897435898 6 21 -3.7532051282051277 6 22 -3.259615384615385 6 23 -4.094551282051281 6 24 -3.668269230769231 6 25 -4.112179487179487 6 26 -3.3381410256410255 6 27 -3.5464743589743595 6 28 -3.653846153846154 6 29 -2.7628205128205128 6 30 -3.4246794871794872 6 31 -3.394230769230769 6 32 -2.967948717948717 6 33 -2.221153846153846 6 34 -2.8589743589743595 6 35 -2.447115384615385 6 36 -2.5689102564102564 6 37 -2.782051282051281 6 38 -2.354166666666667 6 39 -2.30128205128205 6 40 -1.9679487179487172 7 1 4.200320512820511 7 2 -8.665064102564102 7 3 7.139423076923077 7 4 8.89423076923077 7 5 13.036858974358974 7 6 -12.041666666666668 7 7 -10.681089743589743 7 8 -10.528846153846153 7 9 -9.538461538461538 7 10 -6.8157051282051295 7 11 -6.865384615384615 7 12 -6.5400641025641 7 13 -6.9375 7 14 -7.076923076923077 7 15 -7.153846153846153 7 16 -5.108974358974358 7 17 -4.4935897435897445 7 18 -4.834935897435896 7 19 -4.443910256410257 7 20 -4.459935897435898 7 21 -3.7532051282051277 7 22 -3.259615384615385 7 23 -4.094551282051281 7 24 -3.668269230769231 7 25 -4.112179487179487 7 26 -3.3381410256410255 7 27 -3.5464743589743595 7 28 -3.653846153846154 7 29 -2.7628205128205128 7 30 -3.4246794871794872 7 31 -3.394230769230769 7 32 -2.967948717948717 7 33 -2.221153846153846 7 34 -2.8589743589743595 7 35 -2.447115384615385 7 36 -2.5689102564102564 7 37 -2.782051282051281 7 38 -2.354166666666667 7 39 -2.30128205128205 7 40 -1.9679487179487172 8 1 -6.799679487179489 8 2 0.8349358974358978 8 3 -1.3605769230769234 8 4 -8.60576923076923 8 5 -8.463141025641026 8 6 0.9583333333333321 8 7 -10.681089743589743 8 8 -10.528846153846153 8 9 -9.538461538461538 8 10 -6.8157051282051295 8 11 -6.865384615384615 8 12 -6.5400641025641 8 13 -6.9375 8 14 -7.076923076923077 8 15 -7.153846153846153 8 16 -5.108974358974358 8 17 -4.4935897435897445 8 18 -4.834935897435896 8 19 -4.443910256410257 8 20 -4.459935897435898 8 21 -3.7532051282051277 8 22 -3.259615384615385 8 23 -4.094551282051281 8 24 -3.668269230769231 8 25 -4.112179487179487 8 26 -3.3381410256410255 8 27 -3.5464743589743595 8 28 -3.653846153846154 8 29 -2.7628205128205128 8 30 -3.4246794871794872 8 31 -3.394230769230769 8 32 -2.967948717948717 8 33 -2.221153846153846 8 34 -2.8589743589743595 8 35 -2.447115384615385 8 36 -2.5689102564102564 8 37 -2.782051282051281 8 38 -2.354166666666667 8 39 -2.30128205128205 8 40 -1.9679487179487172 9 1 11.200320512820511 9 2 17.334935897435898 9 3 19.139423076923077 9 4 17.89423076923077 9 5 20.036858974358974 9 6 21.958333333333332 9 7 23.318910256410255 9 8 23.471153846153847 9 9 24.46153846153846 9 10 27.18429487179487 9 11 -6.865384615384615 9 12 -6.5400641025641 9 13 -6.9375 9 14 -7.076923076923077 9 15 -7.153846153846153 9 16 -5.108974358974358 9 17 -4.4935897435897445 9 18 -4.834935897435896 9 19 -4.443910256410257 9 20 -4.459935897435898 9 21 -3.7532051282051277 9 22 -3.259615384615385 9 23 -4.094551282051281 9 24 -3.668269230769231 9 25 -4.112179487179487 9 26 -3.3381410256410255 9 27 -3.5464743589743595 9 28 -3.653846153846154 9 29 -2.7628205128205128 9 30 -3.4246794871794872 9 31 -3.394230769230769 9 32 -2.967948717948717 9 33 -2.221153846153846 9 34 -2.8589743589743595 9 35 -2.447115384615385 9 36 -2.5689102564102564 9 37 -2.782051282051281 9 38 -2.354166666666667 9 39 -2.30128205128205 9 40 -1.9679487179487172 10 1 -22.79967948717949 10 2 -14.665064102564102 10 3 -14.860576923076923 10 4 -14.10576923076923 10 5 -13.963141025641026 10 6 -12.041666666666668 10 7 -10.681089743589743 10 8 -10.528846153846153 10 9 -9.538461538461538 10 10 -6.8157051282051295 10 11 -6.865384615384615 10 12 -6.5400641025641 10 13 -6.9375 10 14 -7.076923076923077 10 15 -7.153846153846153 10 16 -5.108974358974358 10 17 -4.4935897435897445 10 18 -4.834935897435896 10 19 -4.443910256410257 10 20 -4.459935897435898 10 21 -3.7532051282051277 10 22 -3.259615384615385 10 23 -4.094551282051281 10 24 -3.668269230769231 10 25 -4.112179487179487 10 26 -3.3381410256410255 10 27 -3.5464743589743595 10 28 -3.653846153846154 10 29 -2.7628205128205128 10 30 -3.4246794871794872 10 31 -3.394230769230769 10 32 -2.967948717948717 10 33 -2.221153846153846 10 34 -2.8589743589743595 10 35 -2.447115384615385 10 36 -2.5689102564102564 10 37 -2.782051282051281 10 38 -2.354166666666667 10 39 -2.30128205128205 10 40 -1.9679487179487172 11 1 6.200320512820511 11 2 14.334935897435898 11 3 0.13942307692307665 11 4 3.89423076923077 11 5 2.0368589743589745 11 6 -7.041666666666668 11 7 16.318910256410255 11 8 7.471153846153847 11 9 13.461538461538462 11 10 -0.8157051282051295 11 11 6.134615384615385 11 12 7.4599358974359 11 13 14.0625 11 14 16.923076923076923 11 15 10.846153846153847 11 16 25.891025641025642 11 17 14.506410256410255 11 18 16.165064102564102 11 19 19.556089743589745 11 20 26.540064102564102 11 21 14.246794871794872 11 22 14.740384615384615 11 23 7.905448717948719 11 24 7.331730769230769 11 25 15.887820512820513 11 26 2.6618589743589745 11 27 15.45352564102564 11 28 25.346153846153847 11 29 8.237179487179487 11 30 9.575320512820513 11 31 23.60576923076923 11 32 -2.967948717948717 11 33 -2.221153846153846 11 34 -2.8589743589743595 11 35 -2.447115384615385 11 36 -2.5689102564102564 11 37 -2.782051282051281 11 38 -2.354166666666667 11 39 -2.30128205128205 11 40 -1.9679487179487172 12 1 -22.79967948717949 12 2 -14.665064102564102 12 3 -14.860576923076923 12 4 -14.10576923076923 12 5 -13.963141025641026 12 6 -12.041666666666668 12 7 -10.681089743589743 12 8 -10.528846153846153 12 9 -9.538461538461538 12 10 -6.8157051282051295 12 11 -6.865384615384615 12 12 -6.5400641025641 12 13 -6.9375 12 14 -7.076923076923077 12 15 -7.153846153846153 12 16 -5.108974358974358 12 17 -4.4935897435897445 12 18 -4.834935897435896 12 19 -4.443910256410257 12 20 -4.459935897435898 12 21 -3.7532051282051277 12 22 -3.259615384615385 12 23 -4.094551282051281 12 24 -3.668269230769231 12 25 -4.112179487179487 12 26 -3.3381410256410255 12 27 -3.5464743589743595 12 28 -3.653846153846154 12 29 -2.7628205128205128 12 30 -3.4246794871794872 12 31 -3.394230769230769 12 32 -2.967948717948717 12 33 -2.221153846153846 12 34 -2.8589743589743595 12 35 -2.447115384615385 12 36 -2.5689102564102564 12 37 -2.782051282051281 12 38 -2.354166666666667 12 39 -2.30128205128205 12 40 -1.9679487179487172 13 1 8.200320512820511 13 2 6.334935897435898 13 3 12.139423076923077 13 4 14.39423076923077 13 5 15.536858974358974 13 6 8.458333333333332 13 7 4.318910256410257 13 8 4.971153846153847 13 9 1.4615384615384617 13 10 4.1842948717948705 13 11 0.13461538461538503 13 12 4.4599358974359 13 13 4.5625 13 14 -1.0769230769230766 13 15 5.846153846153847 13 16 9.391025641025642 13 17 8.506410256410255 13 18 -1.834935897435896 13 19 7.556089743589743 13 20 1.0400641025641022 13 21 4.246794871794872 13 22 -0.259615384615385 13 23 3.905448717948719 13 24 3.831730769230769 13 25 8.387820512820513 13 26 4.1618589743589745 13 27 6.4535256410256405 13 28 3.846153846153846 13 29 0.23717948717948723 13 30 2.0753205128205128 13 31 5.105769230769231 13 32 6.032051282051283 13 33 6.778846153846154 13 34 10.14102564102564 13 35 9.052884615384615 13 36 13.931089743589745 13 37 11.217948717948719 13 38 9.145833333333332 13 39 14.198717948717949 13 40 12.532051282051283 14 1 4.700320512820511 14 2 8.334935897435898 14 3 3.1394230769230766 14 4 3.39423076923077 14 5 5.5368589743589745 14 6 -4.541666666666668 14 7 -7.181089743589743 14 8 4.471153846153847 14 9 4.461538461538462 14 10 16.68429487179487 14 11 6.134615384615385 14 12 17.959935897435898 14 13 6.0625 14 14 5.423076923076923 14 15 12.346153846153847 14 16 5.891025641025642 14 17 9.006410256410255 14 18 14.165064102564104 14 19 6.556089743589743 14 20 9.040064102564102 14 21 15.746794871794872 14 22 15.740384615384615 14 23 11.405448717948719 14 24 17.83173076923077 14 25 14.387820512820513 14 26 16.661858974358974 14 27 1.9535256410256405 14 28 0.3461538461538458 14 29 4.237179487179487 14 30 8.075320512820513 14 31 8.60576923076923 14 32 10.032051282051283 14 33 4.778846153846154 14 34 8.64102564102564 14 35 6.552884615384615 14 36 7.931089743589744 14 37 11.717948717948719 14 38 11.645833333333332 14 39 12.698717948717949 14 40 12.032051282051283 15 1 6.200320512820511 15 2 6.334935897435898 15 3 8.139423076923077 15 4 0.89423076923077 15 5 9.036858974358974 15 6 -3.041666666666668 15 7 16.318910256410255 15 8 16.471153846153847 15 9 8.461538461538462 15 10 2.1842948717948705 15 11 10.134615384615385 15 12 8.4599358974359 15 13 16.0625 15 14 -3.0769230769230766 15 15 11.846153846153847 15 16 23.891025641025642 15 17 16.506410256410255 15 18 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29 -2.7628205128205128 116 30 -3.4246794871794872 116 31 -3.394230769230769 116 32 -2.967948717948717 116 33 -2.221153846153846 116 34 -2.8589743589743595 116 35 -2.447115384615385 116 36 -2.5689102564102564 116 37 -2.782051282051281 116 38 -2.354166666666667 116 39 -2.30128205128205 116 40 -1.9679487179487172 117 1 9.700320512820511 117 2 17.334935897435898 117 3 13.639423076923077 117 4 18.39423076923077 117 5 13.036858974358974 117 6 8.958333333333332 117 7 16.818910256410255 117 8 19.471153846153847 117 9 19.96153846153846 117 10 25.18429487179487 117 11 7.634615384615385 117 12 7.9599358974359 117 13 -3.9375 117 14 7.423076923076923 117 15 -4.153846153846153 117 16 8.891025641025642 117 17 9.006410256410255 117 18 -4.834935897435896 117 19 -4.443910256410257 117 20 -4.459935897435898 117 21 -3.7532051282051277 117 22 -3.259615384615385 117 23 -4.094551282051281 117 24 -3.668269230769231 117 25 -4.112179487179487 117 26 -3.3381410256410255 117 27 -3.5464743589743595 117 28 -3.653846153846154 117 29 -2.7628205128205128 117 30 -3.4246794871794872 117 31 -3.394230769230769 117 32 -2.967948717948717 117 33 -2.221153846153846 117 34 -2.8589743589743595 117 35 -2.447115384615385 117 36 -2.5689102564102564 117 37 -2.782051282051281 117 38 -2.354166666666667 117 39 -2.30128205128205 117 40 -1.9679487179487172 119 1 5.200320512820511 119 2 5.334935897435898 119 3 -1.8605769230769234 119 4 3.89423076923077 119 5 0.03685897435897445 119 6 6.958333333333332 119 7 15.318910256410257 119 8 8.471153846153847 119 9 6.461538461538462 119 10 16.18429487179487 119 11 13.134615384615385 119 12 3.4599358974358996 119 13 10.0625 119 14 10.923076923076923 119 15 21.846153846153847 119 16 10.891025641025642 119 17 14.506410256410255 119 18 14.165064102564104 119 19 1.5560897435897427 119 20 19.540064102564102 119 21 16.246794871794872 119 22 1.740384615384615 119 23 8.905448717948719 119 24 8.33173076923077 119 25 4.887820512820513 119 26 0.6618589743589745 119 27 19.453525641025642 119 28 15.346153846153847 119 29 20.23717948717949 119 30 14.575320512820513 119 31 21.60576923076923 119 32 25.032051282051285 119 33 23.778846153846153 119 34 27.141025641025642 119 35 25.552884615384613 119 36 -2.5689102564102564 119 37 -2.782051282051281 119 38 -2.354166666666667 119 39 -2.30128205128205 119 40 -1.9679487179487172 120 1 3.200320512820511 120 2 -14.665064102564102 120 3 -14.860576923076923 120 4 -14.10576923076923 120 5 -13.963141025641026 120 6 -12.041666666666668 120 7 -10.681089743589743 120 8 -10.528846153846153 120 9 -9.538461538461538 120 10 -6.8157051282051295 120 11 -6.865384615384615 120 12 -6.5400641025641 120 13 -6.9375 120 14 -7.076923076923077 120 15 -7.153846153846153 120 16 -5.108974358974358 120 17 -4.4935897435897445 120 18 -4.834935897435896 120 19 -4.443910256410257 120 20 -4.459935897435898 120 21 -3.7532051282051277 120 22 -3.259615384615385 120 23 -4.094551282051281 120 24 -3.668269230769231 120 25 -4.112179487179487 120 26 -3.3381410256410255 120 27 -3.5464743589743595 120 28 -3.653846153846154 120 29 -2.7628205128205128 120 30 -3.4246794871794872 120 31 -3.394230769230769 120 32 -2.967948717948717 120 33 -2.221153846153846 120 34 -2.8589743589743595 120 35 -2.447115384615385 120 36 -2.5689102564102564 120 37 -2.782051282051281 120 38 -2.354166666666667 120 39 -2.30128205128205 120 40 -1.9679487179487172 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 434.0 5 56.0 6 91.0 7 132.0 8 113.0 9 60.0 10 73.0 11 52.0 12 48.0 13 46.0 14 15.0 15 26.0 16 11.0 17 11.0 18 14.0 19 28.0 20 26.0 21 34.0 22 30.0 23 37.0 24 24.0 25 30.0 26 15.0 27 14.0 28 5.0 29 2.0 30 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.16142557651992 0.1397624039133473 0.4891684136967156 0.20964360587002098 8 97.48427672955975 1.1180992313067784 1.1180992313067784 0.2795248078266946 9 98.32050384884535 0.5598320503848845 0.8397480755773268 0.27991602519244224 10 68.25174825174825 16.363636363636363 6.643356643356643 8.741258741258742 11 44.793850454227815 21.523410202655484 22.292103424178897 11.390635918937805 12 35.84905660377358 18.37875611460517 25.85604472396925 19.91614255765199 13 31.027253668763105 21.45352900069881 24.388539482879107 23.13067784765898 14 21.17400419287212 25.57651991614256 31.516422082459815 21.733053808525508 15 25.995807127882596 25.366876310272534 30.538085255066388 18.099231306778478 16 40.041928721174 23.340321453529 21.45352900069881 15.164220824598182 17 31.586303284416488 26.41509433962264 27.393431167016075 14.605171208944794 18 31.097134870719778 25.22711390635919 29.350104821802937 14.3256464011181 19 30.468204053109716 30.398322851153043 22.92103424178896 16.21243885394829 20 27.53319357092942 31.236897274633122 25.925925925925924 15.30398322851153 21 27.67295597484277 28.162124388539482 25.78616352201258 18.37875611460517 22 22.501747030048914 24.6680642907058 30.468204053109716 22.36198462613557 23 23.846153846153847 25.524475524475527 30.20979020979021 20.41958041958042 24 28.162124388539482 22.57162823200559 30.81761006289308 18.448637316561843 25 25.858444288717592 27.820602662929222 25.43798177995795 20.882971268395234 26 22.05056179775281 26.474719101123593 26.75561797752809 24.719101123595504 27 22.05056179775281 26.40449438202247 27.3876404494382 24.15730337078652 28 19.79020979020979 31.11888111888112 24.755244755244753 24.335664335664337 29 27.055516514406186 29.093464511595222 23.190442726633872 20.660576247364723 30 24.2296918767507 24.579831932773107 31.442577030812323 19.747899159663866 31 25.682295311406577 25.822253324002798 26.801959412176345 21.693491952414277 32 31.394533987386126 23.615977575332867 23.405746320953046 21.58374211632796 33 28.09224318658281 25.296995108315862 24.87770789657582 21.733053808525508 34 24.947589098532493 25.506638714185886 30.81761006289308 18.72816212438854 35 23.340321453529 29.629629629629626 30.18867924528302 16.84136967155835 36 18.977591036414566 36.27450980392157 24.29971988795518 20.448179271708682 37 25.296995108315862 33.05380852550664 25.157232704402517 16.49196366177498 38 25.366876310272534 29.979035639413 26.275331935709296 18.37875611460517 39 25.78616352201258 30.538085255066388 21.313766596785463 22.36198462613557 40 25.57651991614256 25.85604472396925 25.925925925925924 22.641509433962266 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.5 17 3.0 18 3.0 19 5.5 20 8.0 21 5.5 22 3.0 23 3.0 24 4.5 25 6.0 26 7.0 27 8.0 28 8.0 29 14.0 30 20.0 31 22.0 32 24.0 33 24.0 34 31.0 35 38.0 36 41.5 37 45.0 38 45.0 39 51.0 40 57.0 41 60.0 42 63.0 43 63.0 44 80.5 45 98.0 46 106.5 47 115.0 48 115.0 49 121.5 50 128.0 51 148.0 52 168.0 53 168.0 54 136.5 55 105.0 56 122.0 57 139.0 58 139.0 59 145.5 60 152.0 61 146.5 62 141.0 63 141.0 64 100.0 65 59.0 66 43.5 67 28.0 68 28.0 69 20.5 70 13.0 71 9.5 72 6.0 73 6.0 74 4.0 75 2.0 76 1.5 77 1.0 78 1.0 79 1.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.1397624039133473 10 0.06988120195667365 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.06988120195667365 24 0.0 25 0.2795248078266946 26 0.4891684136967156 27 0.4891684136967156 28 0.06988120195667365 29 0.5590496156533892 30 0.20964360587002098 31 0.1397624039133473 32 0.2795248078266946 33 0.0 34 0.0 35 0.0 36 0.20964360587002098 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1431.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.09853249475891 #Duplication Level Percentage of deduplicated Percentage of total 1 93.25490196078431 83.08874912648497 2 5.019607843137255 8.944793850454227 3 0.9411764705882352 2.515723270440252 4 0.39215686274509803 1.397624039133473 5 0.1568627450980392 0.6988120195667366 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.2352941176470588 3.3542976939203357 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTGTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 18 1.257861635220126 No Hit CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 17 1.187980433263452 No Hit CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 13 0.9084556254367574 No Hit CTGTGTGGGGGGGAGGTAGTGACGAAAAATAACAATACAG 5 0.3494060097833683 No Hit CTGTGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC 5 0.3494060097833683 No Hit CTGTGTGGGGGTGTAGCTCAGTTGGTAAGTAGTTGCCTAG 4 0.2795248078266946 No Hit CTGTGTGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAG 4 0.2795248078266946 No Hit CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC 4 0.2795248078266946 No Hit CTGTGTGGGGTTTGATCGTACTAAAAACTCTTAAGAAAAA 4 0.2795248078266946 No Hit CTGTGTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAG 4 0.2795248078266946 No Hit CTGTGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 3 0.20964360587002098 No Hit CTGTGTGGGATCACCATGAAGGTCGAGCTGTGCAGTTTCA 3 0.20964360587002098 No Hit CTGTGTGGGGACATTAAGCCAACTCCAGGATTCGGGAGTT 3 0.20964360587002098 No Hit CTGTGTGGGATTGTATTGTCTCACACATGCTCTATATAAA 3 0.20964360587002098 No Hit CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG 3 0.20964360587002098 No Hit CTGTGTGGGGTTTTCATATTGATAAAATTATTATTGCTTC 3 0.20964360587002098 No Hit CTGTGTGGGGCTGTCAGTTGTTTTATTGATCTCAGCCACT 3 0.20964360587002098 No Hit CTGTGTGGGGACTCTGATTCCGACACATGACTCCGAGGTG 3 0.20964360587002098 No Hit CTGTGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGG 3 0.20964360587002098 No Hit CTGTGTGGGCTGTTAGTAGGATTCTTACGTTTGCCTTTCG 3 0.20964360587002098 No Hit CTGTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCG 3 0.20964360587002098 No Hit CTGTGTGGGGGAGAGGGACTCTTCAGTATGGAGGCAGCCT 3 0.20964360587002098 No Hit CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAG 2 0.1397624039133473 No Hit CTGTGTGGGGGTTTGGGGCTAGGTTGGTAGGAAGTTTACC 2 0.1397624039133473 No Hit CTGTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.1397624039133473 No Hit CTGTGTGGGGGGGTGGGGATAACCTGTGAGTGCAGCCGGG 2 0.1397624039133473 No Hit CTGTGTGGGGGACATGATAGCAGTTACCTGCATTCCTGTC 2 0.1397624039133473 No Hit CTGTGTGGGGAGAGTGAGTTCCAGGACAGCCAGGGCTACA 2 0.1397624039133473 No Hit CTGTGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCT 2 0.1397624039133473 No Hit CTGTGTGGAGGAGGTTTTGATTTATTTCCTCTTCTTGCTC 2 0.1397624039133473 No Hit CTGTGTGGGGCTTTCCGAGCTGCTAGCGCTCCTGCGAACA 2 0.1397624039133473 No Hit CTGTGTGGGAGTCCTCATTAAGACACAAAGGCCTGGGTGG 2 0.1397624039133473 No Hit CTGTGTGGGGGGCTGGAGAGATGGCTCAGCAGTTATTAGC 2 0.1397624039133473 No Hit CTGTGTGGGGAGTCGAGCCCTATCTCAAGTCACTAATGCT 2 0.1397624039133473 No Hit CTGTGTGGGCGGGGAGGTAGTGACGAAAAATAACAATACA 2 0.1397624039133473 No Hit CTGTGTGGGCCTAATCCTAGCCCTAGCCCTACACAAATAT 2 0.1397624039133473 No Hit CTGTGTGATTGTATATGTTTGTGTAAATGTGTGTGTAAAT 2 0.1397624039133473 No Hit CTGTGTGGGGGGAAGGGGTGGATTAGAAACAAAAATTCTG 2 0.1397624039133473 No Hit CTGTGTGGGGGGCTCTGTTTGCTACAGAAAAGAGTGAAAC 2 0.1397624039133473 No Hit CTGTGTGGGGGTCGTGGTGTGCCCAGCTCTTCCAAGGACT 2 0.1397624039133473 No Hit CTGTGTGGGGACTGCTGGAGGAAGATTGCAAAAGATGAAG 2 0.1397624039133473 No Hit CTGTGTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAAAGC 2 0.1397624039133473 No Hit CTGTGTGGGGTAGTGGTTGGTGTTGGCAGAGCTGGCTCTG 2 0.1397624039133473 No Hit CTGTGTGGGGACTGGGGTTCTCACAGGGAATCCCCCTTGC 2 0.1397624039133473 No Hit CTGTGTGGGGGCTGGCTAGATGGCTCAGCGGATAAGAGCA 2 0.1397624039133473 No Hit CTGTGTGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT 2 0.1397624039133473 No Hit CTGTGTGGGGGTCCATAGTGGGTTCAGGGATGAAGCTTGC 2 0.1397624039133473 No Hit CTGTGTGGGGTGGCCATCTTCCACAAGTACTCTGGCAAGG 2 0.1397624039133473 No Hit CTGTGTGGGGGACGAGAGGCTTTGTCCGGTTCGGCGGTCT 2 0.1397624039133473 No Hit CTGTGTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC 2 0.1397624039133473 No Hit CTGTGTGGGGGGCTGGAGAGATGGCTCAGCGGTTGAGAGC 2 0.1397624039133473 No Hit CTGTGTGGGATACATTTAAGATTTTTTTTAAGGTAACAGA 2 0.1397624039133473 No Hit CTGTGTGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAGC 2 0.1397624039133473 No Hit CTGTGTGGGTGTGTTCTATATTCCAAATTAACTAGGCCTA 2 0.1397624039133473 No Hit CTGTGTGGGGAATGTTTGAAGTTCTCAGTTATTGTTTACT 2 0.1397624039133473 No Hit CTGTGTGGGGAGAAGGTAAAAACTCTAATTTTCTATCAAA 2 0.1397624039133473 No Hit CTGTGTGGGATGGCTCGTATGCCGTCTTCTGCTTGGATCG 2 0.1397624039133473 Illumina Single End Adapter 2 (95% over 24bp) CTGTGTGGGGACCCTGAGGGACCTCTAGAGCATTTCTGAG 2 0.1397624039133473 No Hit CTGTGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGC 2 0.1397624039133473 No Hit CTGTGTGGGATTGTATGTGGCCTGGACTCTATCATAGCGA 2 0.1397624039133473 No Hit CTGTGTGGGGGCTGGGACTCCTACTCCACCCTAGCAGTTC 2 0.1397624039133473 No Hit CTGTGTGGGGGGACTGGAGAGATGGCTCAGCGGTTAAGAG 2 0.1397624039133473 No Hit CTGTGTGGGGACATCACTAATGAGGAATATGGAGAGTTCT 2 0.1397624039133473 No Hit CTGTGTTTGTGGGAGATGGTTCTTTTTTTGATTCTAATAA 2 0.1397624039133473 No Hit CTGTGTGGGGAAGGTAAGCATCATTGTACCTTTAATATGT 2 0.1397624039133473 No Hit CTGTGTGGGGGAAACATTCTATTAATGGTGGCCCGTCTCA 2 0.1397624039133473 No Hit CTGTGTGGGGGCTACCAAATCCAAGGAAGGCAGCAGGCGC 2 0.1397624039133473 No Hit CTGTGTGGGAACCAGGAGCTGCAAGAGATCAACAGAGTGT 2 0.1397624039133473 No Hit CTGTGTGGGGGGTGCCTGGGTGATCCGCCTTGTGGATCGA 2 0.1397624039133473 No Hit CTGTGTGGGGGCTAGACGTACAGCGACTCTGTGTCATTTA 2 0.1397624039133473 No Hit CTGTGTGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTC 2 0.1397624039133473 No Hit CTGTGTGGGGGAGCACCTTTTTACTTTGGTAGGTATGCAG 2 0.1397624039133473 No Hit CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTG 2 0.1397624039133473 No Hit CTGTGTGGGATGTTTTATAAATAAAATGTTTTTCCTCAAA 2 0.1397624039133473 No Hit CTGTGTGGGGGATTTCTGAGTTCGAGGCCAGCCTGGTCTA 2 0.1397624039133473 No Hit CTGTGTGGGATTTAAGGAGAATTGTGTAAGATAAGCTTGT 2 0.1397624039133473 No Hit CTGTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA 2 0.1397624039133473 No Hit CTGTGTGGGATTTTGGATGATTAGAAGCACAGAATGATCA 2 0.1397624039133473 No Hit CTGTGTGGGGACTTTGTTCTCTTAAGAAAACCCTGGAATT 2 0.1397624039133473 No Hit CTGTGTGGGACTAATCCTAGCCCTAGCCCTACACAAATAT 2 0.1397624039133473 No Hit CTGTGTGGGGGTTTTCAGTCACAACACTTTCACTGTTTGT 2 0.1397624039133473 No Hit CTGTGTGAATGTGTGTGCATGTGTATGGGGGATGTAAAAC 2 0.1397624039133473 No Hit CTGTGTGGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAG 2 0.1397624039133473 No Hit CTGTGTGGGGTATTAGTGGGGGTTGATTTGTGTGTTGGTT 2 0.1397624039133473 No Hit CTGTGTGGGGGGTAACAATATAGATAAAATCCCCATGGAG 2 0.1397624039133473 No Hit CTGTGTGGGGGAGGAGGAGTGAGAGCGGCAAAATGGCGGA 2 0.1397624039133473 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTGTG 140 0.0 34.0 1 TGTGGGG 90 0.0 34.0 4 GTGTGGG 140 0.0 34.0 3 TGTGTGG 140 0.0 34.0 2 GTGGGGG 40 2.0472726E-8 33.999996 5 GTGGGGC 20 0.0019453749 33.999996 5 GTGGGGA 20 0.0019453749 33.999996 5 TGTGGGC 20 0.0019453749 33.999996 4 TGTGGGA 20 0.0019453749 33.999996 4 >>END_MODULE