FastQCFastQC Report
Fri 17 Jun 2016
SRR1526034_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526034_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35437
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT7252.0458842452803565No Hit
GGGCGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC6151.7354742218585093No Hit
GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2350.663148686401219No Hit
GGGCGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1920.5418065863363152No Hit
GGGCGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1300.36684820949854674No Hit
GGGCGAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC1080.30476620481417727No Hit
GGGCGAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT750.2116431977876231No Hit
GGGCGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC720.20317746987611818No Hit
GGGCGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA710.20035556057228318No Hit
GGGCGAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT640.1806021954454384No Hit
GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG640.1806021954454384No Hit
GGGCGAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG630.1777802861416034No Hit
GGGCGAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC620.17495837683776844No Hit
GGGCGAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA560.15802692101475857No Hit
GGGCGAGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT560.15802692101475857No Hit
GGGCGAGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC540.15238310240708863No Hit
GGGCGAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC530.14956119310325366No Hit
GGGCGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC530.14956119310325366No Hit
GGGCGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT520.1467392837994187No Hit
GGGCGAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT510.14391737449558373No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC480.1354516465840788No Hit
GGGCGAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG440.12416400936873888No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC430.12134210006490391No Hit
GGGCGAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC420.11852019076106894No Hit
GGGCGAGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT410.11569828145723397No Hit
GGGCGAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC370.10441064424189406No Hit
GGGCGAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC370.10441064424189406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCCA451.1823431E-1050.4226437
CTCAATC251.9463938E-550.42263840
TGTTCCC203.960011E-450.42263438
GTACAAA150.00822804150.2068545
GACCAAA150.00822804150.2068545
GTACAAG150.0082746350.13532634
CCATCCG150.0082746350.13532642
CTCCGCC252.0135532E-550.13532644
ACTGAAC204.07358E-450.13532342
CAGAACC150.00832141550.0640113
GTCCCAG150.00836839649.99299
GAACTGT355.1013558E-849.99299
GATGGTT150.00836839649.99299
CCTGTAC150.00841557449.9219931
CGGGTAA150.00841557449.9219931
AGCGGGT150.00841557449.9219929
CAGCGGG150.00841557449.9219928
CTGTACA150.00841557449.9219932
GGGCCCT150.00841557449.9219927
GCGGGTA150.00841557449.9219930
Produced by FastQC (version 0.11.5)