##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526034_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35437 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.70793238705308 38.0 35.0 40.0 29.0 40.0 2 35.69743488444281 38.0 35.0 40.0 29.0 40.0 3 35.255354572904025 38.0 35.0 39.0 27.0 40.0 4 35.111550074780595 38.0 35.0 39.0 25.0 40.0 5 35.1331094618619 38.0 35.0 39.0 27.0 40.0 6 32.199621864153286 38.0 30.0 39.0 12.0 40.0 7 35.089115895815105 38.0 35.0 39.0 27.0 40.0 8 34.97502610266106 38.0 35.0 39.0 26.0 40.0 9 35.10003668482095 38.0 35.0 39.0 27.0 40.0 10 34.09746874735446 38.0 33.0 39.0 22.0 40.0 11 33.88266501114654 38.0 32.0 39.0 23.0 40.0 12 33.190761068939246 38.0 31.0 39.0 18.0 40.0 13 32.84956401501256 37.0 31.0 39.0 18.0 40.0 14 33.269040833027624 38.0 31.0 39.0 18.0 40.0 15 32.20244377345712 37.0 30.0 39.0 15.0 40.0 16 32.625137568078564 37.0 30.0 39.0 18.0 40.0 17 31.860174393994978 36.0 28.0 39.0 15.0 40.0 18 31.433896774557667 36.0 27.0 39.0 15.0 40.0 19 32.49659959928888 36.0 30.0 39.0 18.0 40.0 20 33.03801111832266 37.0 31.0 39.0 22.0 40.0 21 33.4832237491887 38.0 32.0 39.0 20.0 40.0 22 32.77393684566978 38.0 31.0 39.0 17.0 40.0 23 33.35922905437819 38.0 32.0 39.0 19.0 40.0 24 32.729943279622994 38.0 31.0 39.0 17.0 40.0 25 33.12306346474024 38.0 31.0 39.0 19.0 40.0 26 33.29827581341536 38.0 33.0 39.0 19.0 40.0 27 33.491999887123626 38.0 33.0 39.0 20.0 40.0 28 33.69890227728081 38.0 33.0 39.0 22.0 40.0 29 33.07737675311115 38.0 32.0 39.0 18.0 40.0 30 32.995484945113866 38.0 32.0 39.0 18.0 40.0 31 33.62206168693738 38.0 33.0 39.0 20.0 40.0 32 33.56311200158027 38.0 33.0 39.0 20.0 40.0 33 33.27033891130739 38.0 33.0 39.0 19.0 40.0 34 33.148234895730454 37.0 33.0 39.0 19.0 40.0 35 33.141688066145555 38.0 33.0 39.0 18.0 40.0 36 32.570138555746816 37.0 32.0 38.0 17.0 40.0 37 32.31382453367949 36.0 31.0 38.0 14.0 40.0 38 31.636904929875552 36.0 30.0 38.0 10.0 40.0 39 30.521827468465162 35.0 28.0 38.0 4.0 40.0 40 28.397832773654656 34.0 22.0 38.0 4.0 39.0 41 26.11067528289641 31.0 15.0 37.0 4.0 39.0 42 27.01267037277422 32.0 20.0 37.0 4.0 39.0 43 27.45881423371053 33.0 21.0 38.0 4.0 39.0 44 26.484380732003274 32.0 17.0 38.0 4.0 39.0 45 27.321217936055536 33.0 21.0 38.0 4.0 39.0 46 25.40562124333324 30.0 15.0 36.0 4.0 39.0 47 24.52445184411773 29.0 11.0 36.0 4.0 39.0 48 24.994215085927138 30.0 14.0 36.0 4.0 39.0 49 24.073595394644016 29.0 9.0 36.0 4.0 39.0 50 24.84612128566188 30.0 11.0 36.0 4.0 39.0 51 24.93825662443209 30.0 4.0 36.0 4.0 39.0 52 24.77596862036854 30.0 4.0 36.0 4.0 39.0 53 24.620255664982928 30.0 4.0 36.0 4.0 39.0 54 24.22863109179671 30.0 4.0 36.0 4.0 39.0 55 24.156390213618533 30.0 4.0 36.0 4.0 39.0 56 23.209244574879364 29.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 5.30840983282458 1 2 5.117731873791449 1 3 5.72492240027151 1 4 5.828157881320919 1 5 5.785266843696554 1 6 9.164231820663389 1 7 6.108766969314715 1 8 5.603138774981971 1 9 5.940407734273762 1 10 6.33140731997938 1 11 0.9441969312183822 1 12 -4.9471946667732425 1 13 -18.59370803039873 1 14 -3.886332606942574 1 15 2.750783332077315 1 16 0.11579441259049261 1 17 -4.0898484127027 1 18 -2.9131540511754466 1 19 1.0971559637752826 1 20 0.525463170098881 1 21 6.333048571847897 1 22 6.771826831663489 1 23 5.771919013574454 1 24 7.140044582858735 1 25 6.353258854081012 1 26 6.579837394204834 1 27 6.177136366081424 1 28 6.544671066874564 1 29 7.0413865220952765 1 30 6.271893105387729 1 31 4.925650404629639 1 32 5.47932196601468 1 33 5.243346949673214 1 34 5.253727154621387 1 35 5.88285818886218 1 36 3.8743970816373476 1 37 4.616602697609622 1 38 4.994860066550931 1 39 4.869280183327096 1 40 -12.599756484688827 1 41 0.18053742711050447 1 42 -1.673490847158888 1 43 -7.399266959256334 1 44 -14.461614644170115 1 45 -1.706760591246585 1 46 -4.490366718501999 1 47 -9.672314496057572 1 48 -3.970351077453415 1 49 -13.149005790343232 1 50 -2.7987836815988274 1 51 2.545927816496601 1 52 2.142005523135598 1 53 2.335784953551496 1 54 3.129517839554701 1 55 2.7272230197890472 1 56 6.031802005054892 2 1 4.891986688464556 2 2 4.993243270635396 2 3 5.530883826338133 2 4 5.598759868462942 2 5 5.486026633293285 2 6 8.769433457133289 2 7 5.6911749178827975 2 8 5.9164058702479 2 9 5.467000370159212 2 10 6.312704806829171 2 11 3.5052723257245226 2 12 -0.46326713901169825 2 13 0.41096759788882053 2 14 5.701627650216981 2 15 5.540964512673458 2 16 4.381720771444964 2 17 6.671694544632189 2 18 3.9687863345638874 2 19 5.82450838925746 2 20 4.6175146020100435 2 21 5.28892232988413 2 22 6.480768970763435 2 23 6.163210655888889 2 24 7.368565915412802 2 25 6.649284570036592 2 26 5.5707783643977 2 27 5.340198902609767 2 28 5.630877963426286 2 29 6.466576469494459 2 30 6.633669844137 2 31 3.5922196623736546 2 32 4.7548918082122285 2 33 4.563335260602496 2 34 5.019069059939916 2 35 5.054103074893746 2 36 4.449499361006133 2 37 4.679723679491566 2 38 4.773644403196169 2 39 5.280151019095651 2 40 2.3701441582100635 2 41 8.209467877139726 2 42 6.612599158685647 2 43 6.125280089253309 2 44 -4.1252616342344055 2 45 6.409253435638277 2 46 5.44768120668795 2 47 1.5576679703363503 2 48 4.298205322312803 2 49 -2.8646691451065287 2 50 5.740082478541442 2 51 8.422900347180413 2 52 8.078884541253657 2 53 7.891892493002111 2 54 8.777384584148507 2 55 8.109747859064328 2 56 9.14226372334829 3 1 4.608493189312497 3 2 4.469218397825898 3 3 5.0775203441448085 3 4 4.936521315608225 3 5 5.436281014299503 3 6 8.701881167704236 3 7 5.460253493349164 3 8 5.6139751184107 3 9 5.365247967671927 3 10 5.481726853183044 3 11 5.483791262977203 3 12 5.968615280434317 3 13 6.020351430562648 3 14 6.290661002393353 3 15 0.1333896115994051 3 16 6.249442648211495 3 17 6.904594488103079 3 18 7.2457789857792605 3 19 6.173010367776392 3 20 5.252053889743227 3 21 5.925157490992106 3 22 6.964658173702944 3 23 6.601593923271594 3 24 6.082528606198551 3 25 6.036226345050721 3 26 5.711818500067569 3 27 5.408033837601291 3 28 5.626638279989315 3 29 5.347582687696832 3 30 5.747858651372727 3 31 3.7564367341656215 3 32 4.332336692327544 3 33 3.0282872108568775 3 34 4.158978613359928 3 35 4.278523651490694 3 36 4.63321897660817 3 37 5.1687005025554456 3 38 5.349958705061628 3 39 6.005984823504921 3 40 8.12961278455263 3 41 10.682899194268046 3 42 8.946403567956423 3 43 8.226184555053198 3 44 7.816796025460338 3 45 8.187376669103514 3 46 9.840558538513843 3 47 9.644440158013001 3 48 9.469771178728855 3 49 10.523403212157465 3 50 10.586323292560664 3 51 9.96020956142575 3 52 8.721055259173387 3 53 8.655318157219185 3 54 8.717181079343533 3 55 7.868651194281963 3 56 7.625022344037944 4 1 4.30840983282458 4 2 4.4397657720965285 4 3 5.131702061288458 4 4 5.489174830473466 4 5 4.6666227758999455 4 6 9.401519956256614 4 7 5.820631376094376 4 8 5.569240469897224 4 9 5.51667892071444 4 10 5.585644608114972 4 11 6.384874897320078 4 12 6.781618892548789 4 13 6.575783495025 4 14 6.893328410006578 4 15 5.682986721907827 4 16 7.403930005810832 4 17 8.11354141780577 4 18 8.273286626790657 4 19 7.724274607843078 4 20 6.881395373488711 4 21 6.3499977243902705 4 22 6.31419971301942 4 23 6.5685291830659835 4 24 6.2417394981129775 4 25 6.658343599843725 4 26 5.851023834882799 4 27 5.380526196589894 4 28 5.409077846535581 4 29 6.092233979722398 4 30 5.373588020641964 4 31 3.0442944724262553 4 32 4.835254169404514 4 33 4.599279153063048 4 34 4.592710205468848 4 35 4.696417510896083 4 36 4.603210640959375 4 37 6.023382358626577 4 38 5.571131252991609 4 39 6.716737810445739 4 40 -1.0404344507905208 4 41 10.75680861355118 4 42 8.462102373180095 4 43 8.99056354921824 4 44 10.419741288033272 4 45 9.886459747736467 4 46 11.882514637430209 4 47 11.887007537840734 4 48 11.826259092038107 4 49 11.63065522660592 4 50 11.980877335350328 4 51 11.46118205378474 4 52 10.803022472288141 4 53 10.657818851856582 4 54 11.485450042944528 4 55 11.201799290975487 4 56 11.523327428783706 5 1 4.638371560544648 5 2 3.8864579536164854 5 3 4.3017403335683895 5 4 4.9558232722449205 5 5 4.60894098257026 5 6 7.772214324763993 5 7 4.909751113655879 5 8 5.127195636655017 5 9 5.365776788401703 5 10 4.8032498568847934 5 11 4.546985340185035 5 12 5.355976464992747 5 13 4.32865391602008 5 14 5.528101510061251 5 15 5.758984534920398 5 16 5.8372268893537544 5 17 6.772918126389698 5 18 6.240755188846421 5 19 6.249424963228535 5 20 5.8800285063482 5 21 5.134306089617169 5 22 6.5241505058023606 5 23 5.2382940819178145 5 24 6.595484714077983 5 25 5.861186683495994 5 26 3.911712735921615 5 27 4.4614447313083225 5 28 4.834993647519724 5 29 4.380369572942737 5 30 4.224326128897847 5 31 2.63560119741258 5 32 2.673690473395766 5 33 2.0227345931942615 5 34 2.5090579364693966 5 35 1.9183967345155466 5 36 2.9700777814733215 5 37 3.7942954258764345 5 38 3.6640782185465532 5 39 5.958126547460505 5 40 7.01697396911106 5 41 8.76063584154462 5 42 8.588127523535476 5 43 7.386681646539184 5 44 8.239588198913538 5 45 7.596683913947508 5 46 8.618982652739117 5 47 9.456444388578841 5 48 7.9211196715897785 5 49 9.827210721411827 5 50 9.640527417362357 5 51 7.504101681996875 5 52 7.690119246481689 5 53 8.323483149286872 5 54 8.875827298275315 5 55 8.944008148274559 5 56 9.60889331177988 6 1 5.606406751160485 6 2 5.483525402296841 6 3 5.56097787022528 6 4 5.64233353617454 6 5 5.868471774359115 6 6 9.026173269045515 6 7 5.729105952365558 6 8 5.924556340467333 6 9 6.125307580190558 6 10 6.379789446327603 6 11 6.5654604134988155 6 12 7.057735995784537 6 13 7.626014619832397 6 14 6.9756088414395485 6 15 7.138456675682864 6 16 7.255701962667533 6 17 8.358841880055387 6 18 8.697939939579562 6 19 8.110715285809185 6 20 6.968298301069606 6 21 7.056006969382565 6 22 7.125910036594156 6 23 7.367912850246256 6 24 7.35853456745042 6 25 6.6383127832027355 6 26 6.868897486654756 6 27 4.610418338346445 6 28 6.162852885056381 6 29 6.434915027488191 6 30 6.470660439748283 6 31 5.682815273658921 6 32 5.368998391284329 6 33 5.702206733956725 6 34 5.045714827964993 6 35 5.550654799031676 6 36 5.482101241883875 6 37 6.755585748457079 6 38 7.152641268399929 6 39 7.529680799659918 6 40 5.182369863539215 6 41 12.284389507233769 6 42 9.896925177494424 6 43 10.522458772638888 6 44 10.59354714319506 6 45 9.891698576704108 6 46 11.211944529571962 6 47 12.097793362185879 6 48 11.689741372469541 6 49 12.577650604109774 6 50 10.619706302992853 6 51 10.845157400471948 6 52 10.639386108651777 6 53 10.427002210870448 6 54 10.425973925841294 6 55 10.522600523641128 6 56 11.497749616765216 7 1 4.922490667244396 7 2 4.9919170106884465 7 3 5.3830058422532545 7 4 5.5370887809297855 7 5 5.371968277855622 7 6 9.177921520793767 7 7 5.290644413904019 7 8 6.068262634173628 7 9 5.686496391379372 7 10 5.704614621152782 7 11 6.594131742170145 7 12 5.619624107672653 7 13 7.308182452000224 7 14 5.935049400880107 7 15 7.862582549808739 7 16 7.749757906723477 7 17 8.625275446488956 7 18 8.511226652866277 7 19 7.218407593501489 7 20 6.667249350020658 7 21 5.853909067284661 7 22 6.922413533097647 7 23 6.3846960670294735 7 24 7.27042267933853 7 25 6.5106904055803625 7 26 4.162953430710697 7 27 4.834893862821968 7 28 5.35236337456687 7 29 5.688061880635043 7 30 6.17345764254549 7 31 4.380018070861713 7 32 3.207809580095514 7 33 5.068640300390296 7 34 4.21005049230066 7 35 3.739116337493215 7 36 4.845387955170594 7 37 5.051739594610929 7 38 5.786907002665661 7 39 6.365694785673906 7 40 5.18446776563712 7 41 10.40641747926174 7 42 10.964058044627293 7 43 8.734696534876651 7 44 11.222218471866391 7 45 9.372118157123687 7 46 11.6990074666349 7 47 12.27716399155651 7 48 12.472608505336673 7 49 12.821007247466415 7 50 11.812363645650194 7 51 10.335367190681737 7 52 11.245330164595835 7 53 11.081547665415904 7 54 10.880169730037103 7 55 11.598474649515254 7 56 9.836560805576404 8 1 4.9700431147968445 8 2 4.756252675024115 8 3 5.19734150658892 8 4 5.060515354356362 8 5 5.595744501631842 8 6 8.935264178136428 8 7 4.983651406911015 8 8 5.597283613194605 8 9 5.743489398372375 8 10 6.088880355418514 8 11 6.256369504407903 8 12 7.166826904875446 8 13 6.753287347105122 8 14 7.1392452050759125 8 15 6.738456675682865 8 16 7.110247417212992 8 17 8.122478243691752 8 18 8.279758121397741 8 19 6.637988013081909 8 20 7.077389210160515 8 21 6.92873424210984 8 22 7.816819127503244 8 23 7.1315492138826215 8 24 7.958534567450414 8 25 7.62013096502092 8 26 6.596170213927483 8 27 6.046781974710083 8 28 6.162852885056381 8 29 7.034915027488193 8 30 7.379751348839196 8 31 4.628269819113466 8 32 5.696271118557057 8 33 5.174934006684001 8 34 5.791169373419542 8 35 6.096109344486223 8 36 5.3730103327929655 8 37 6.155585748457085 8 38 7.025368541127204 8 39 7.656953526932643 8 40 1.3096425908119436 8 41 11.866207689051954 8 42 9.62419790476715 8 43 10.795186045366162 8 44 10.70263805228597 8 45 10.909880394885924 8 46 13.53921725684469 8 47 13.734156998549512 8 48 14.289741372469543 8 49 14.850377876837047 8 50 14.05606993935649 8 51 11.972430127744673 8 52 12.039386108651776 8 53 13.154274938143175 8 54 11.571428471295839 8 55 12.06805506909567 8 56 12.715931434947038 9 1 5.20605024492162 9 2 4.69101203331288 9 3 5.018375374681611 9 4 4.721477921201277 9 5 5.258846105909917 9 6 8.905674160311115 9 7 5.231066736679281 9 8 5.806196269166087 9 9 5.358819166643322 9 10 6.2200746513186935 9 11 5.539078951823239 9 12 6.5928518603121695 9 13 6.5468702348056595 9 14 7.110011693489465 9 15 7.5477258378932 9 16 7.241085206874306 9 17 7.6230130030500405 9 18 7.463715340649081 9 19 6.851891756397414 9 20 6.774715413369069 9 21 6.382566684177576 9 22 6.966908254062957 9 23 6.90053317110187 9 24 6.787768079036866 9 25 5.732786936500418 9 26 5.281731711253688 9 27 4.856764149398142 9 28 5.525063223737305 9 29 4.909781337648621 9 30 5.837861865773238 9 31 5.009731494692783 9 32 5.555094647968822 9 33 4.2330445236180445 9 34 3.2560535088919096 9 35 3.4016351911885394 9 36 5.047876642953398 9 37 5.073232807280611 9 38 4.668505796029162 9 39 6.450536414633181 9 40 2.851888580116757 9 41 7.443747796003823 9 42 9.125445676603157 9 43 8.137788540909835 9 44 9.186773524656736 9 45 8.56442584943138 9 46 9.49501048322615 9 47 10.204388727604776 9 48 8.394554206694142 9 49 10.182998197692662 9 50 8.378351579285187 9 51 8.289364174090487 9 52 9.175571491895983 9 53 9.003116292866885 9 54 9.2591290060552 9 55 9.462350969273928 9 56 9.703453716586964 10 1 5.120952205705933 10 2 4.838070856842293 10 3 5.279159688407098 10 4 5.853242627083631 10 5 5.56119904708639 10 6 9.162536905409155 10 7 5.409105952365557 10 8 5.793647249558241 10 9 5.378034852917828 10 10 6.436153082691245 10 11 5.781824049862454 10 12 6.025008723057265 10 13 5.675105528923304 10 14 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3.9124042721869046 115 37 4.319222112093449 115 38 6.007186722945381 115 39 6.887256557235677 115 40 8.630854712024064 115 41 8.320753143597404 115 42 5.73328881385806 115 43 6.480034530214649 115 44 -1.2125134628655445 115 45 5.5947288797344115 115 46 1.3755808932083262 115 47 -7.132509668117152 115 48 -0.3041980214698512 115 49 -3.5799251534659824 115 50 1.5833426666292176 115 51 2.4390967944113413 115 52 2.287870957136626 115 53 2.2330628169310565 115 54 2.219913319780691 115 55 1.874115675156279 115 56 5.594719313734913 116 1 5.4133331580868855 116 2 5.290451809223242 116 3 5.669635878883291 116 4 5.942766436607442 116 5 5.903103808991148 116 6 9.2053940482663 116 7 5.87672499998461 116 8 5.838409154320146 116 9 6.302796757679737 116 10 6.225676892215052 116 11 4.520871668910072 116 12 5.532627770676317 116 13 5.6351055289233045 116 14 5.8214962873269975 116 15 -1.817820380594192 116 16 4.811113218078791 116 17 6.431136252349759 116 18 6.053351194990817 116 19 6.196429571523467 116 20 5.51981345258476 116 21 5.1542753676509605 116 22 7.248420859104975 116 23 6.791289473622882 116 24 7.409616818532669 116 25 5.969481614371567 116 26 5.98621350397077 116 27 5.648080676008785 116 28 5.758956781160279 116 29 6.404179096752262 116 30 6.479318448406293 116 31 5.2646334554770995 116 32 5.1391282614142 116 33 5.636405868155858 116 34 4.863896646146813 116 35 5.569702418079295 116 36 5.756560116342754 116 37 6.241300034171367 116 38 7.230130445889106 116 39 7.800676470655585 116 40 10.33648241765177 116 41 8.978761801606062 116 42 6.54281262338187 116 43 1.8436708938510122 116 44 0.8394345890825079 116 45 6.3025210875266175 116 46 1.1742821919096258 116 47 -7.677964213571697 116 48 0.9533777361059066 116 49 0.04994497640414508 116 50 2.780312363598913 116 51 6.1815210368355835 116 52 5.660165329430999 116 53 6.10535718922543 116 54 5.5770561769235485 116 55 5.897925198965805 116 56 5.529784248799849 117 1 4.760952205705934 117 2 4.471404190175626 117 3 5.2124930217404355 117 4 5.033242627083631 117 5 5.474532380419724 117 6 8.229203572075818 117 7 5.262439285698889 117 8 5.600313916224906 117 9 5.4647015195845015 117 10 5.549486416024575 117 11 1.1018240498624543 117 12 -6.914991276942732 117 13 -6.031561137743363 117 14 1.5119724778031873 117 15 -3.1130584758322897 117 16 -6.174601067635496 117 17 -0.8926732714597634 117 18 -1.6656964240568044 117 19 1.4440486191425173 117 20 0.6864801192514243 117 21 5.059037272412866 117 22 6.08651609720021 117 23 4.443670426003834 117 24 6.219140628056479 117 25 5.417100661990617 117 26 5.8052611230183935 117 27 5.852842580770687 117 28 5.444671066874562 117 29 6.113702906276071 117 30 6.422175591263436 117 31 5.864633455477101 117 32 5.729604451890388 117 33 6.250691582441579 117 34 5.997229979480146 117 35 5.28398813236501 117 36 6.985131544914182 117 37 7.322252415123749 117 38 8.425368541127202 117 39 8.972105042084156 117 40 11.246006227175581 117 41 8.602571325415589 117 42 7.033288813858061 117 43 -5.789662439482321 117 44 -4.103422553774635 117 45 7.697759182764713 117 46 -0.39714637951894716 117 47 -1.6779642135716983 117 48 0.4867110694392416 117 49 -2.973864547405377 117 50 2.280312363598913 117 51 8.714854370168919 117 52 9.136355805621474 117 53 8.881547665415905 117 54 9.586579986447358 117 55 8.207449008489615 117 56 12.825022344037947 118 1 3.978193585016278 118 2 4.165657063738848 118 3 4.87571141254503 118 4 4.857380558118116 118 5 4.899819736741563 118 6 8.37288173299536 118 7 5.225657676503488 118 8 4.985371387489273 118 9 5.156655542573006 118 10 6.140291013725726 118 11 1.1949274981383162 118 12 -3.256370587287563 118 13 -3.451101367628418 118 14 -6.505268901507158 118 15 -10.286621694223093 118 16 -5.445865435451587 118 17 0.1889359239425339 118 18 -2.6174205619878386 118 19 0.4969221823609047 118 20 -0.05489919109340491 118 21 4.237198191953098 118 22 6.273872419039293 118 23 5.249417552440612 118 24 6.23063488093004 118 25 5.59756043210556 118 26 5.2776749161218355 118 27 5.133302350885632 118 28 5.268808997909048 118 29 6.345886814322046 118 30 5.150911223447341 118 31 4.5404955244426155 118 32 4.910064222005332 118 33 4.821955950257671 118 34 4.725965611664051 118 35 5.260999626617881 118 36 6.4150166023854425 118 37 6.36937885190536 118 38 6.997782334230646 118 39 8.86635791564738 118 40 10.421868296141096 118 41 8.778433394381103 118 42 7.629840537995992 118 43 -0.06437508316048479 118 44 -0.10802025492406031 118 45 9.047184470121039 118 46 -1.0350774140017052 118 47 -0.04578030552572088 118 48 1.1430329085197002 118 49 -0.43363466234790593 118 50 1.3262893750931681 118 51 6.164279657525238 118 52 6.596125920564003 118 53 6.702237320588317 118 54 7.691177687596781 118 55 7.713196134926395 118 56 10.245711999210357 119 1 3.860952205705935 119 2 3.480927999699439 119 3 3.979159688407101 119 4 4.076099769940775 119 5 4.312627618514959 119 6 8.291108333980581 119 7 4.75767738093699 119 8 4.947932963843954 119 9 4.826606281489262 119 10 4.187581654119814 119 11 1.0589669070053098 119 12 -3.729276991228449 119 13 -3.8077516139338385 119 14 -6.302313236482526 119 15 -8.736867999641813 119 16 -9.450791543825972 119 17 -1.4593399381264298 119 18 -7.465696424056802 119 19 -1.6035704284765337 119 20 -1.7278055950342903 119 21 3.263799177174775 119 22 3.1103256210097356 119 23 3.2484323307657377 119 24 2.485807294723145 119 25 3.326624471514428 119 26 4.505261123018389 119 27 4.576652104580209 119 28 2.43038535258885 119 29 4.523226715799883 119 30 4.917413686501529 119 31 3.4360620269056703 119 32 3.1819854042713374 119 33 4.226882058632054 119 34 3.2924680747182435 119 35 3.0363690847459637 119 36 2.5756077353903706 119 37 4.012728605599939 119 38 4.568225683984341 119 39 5.924485994465112 119 40 8.031720512889866 119 41 5.731142753987015 119 42 4.7618602424294885 119 43 0.8055756557557743 119 44 -4.312946363298444 119 45 5.050140135145664 119 46 -3.5542892366618055 119 47 -3.2684404040478867 119 48 -6.660907978179807 119 49 -4.697674071214902 119 50 -1.167306684020133 119 51 4.824378179692726 119 52 4.917308186573855 119 53 4.995833379701619 119 54 4.753246653114022 119 55 4.9407823418229455 119 56 8.225022344037946 120 1 3.370043114796843 120 2 3.9441314629029023 120 3 4.373099082346492 120 4 4.496878990719999 120 5 4.17150207738942 120 6 8.123142966015216 120 7 4.238196861456466 120 8 4.4639502798612725 120 9 4.54955000443298 120 10 4.082819749357906 120 11 0.6745513225897248 120 12 -1.7725670345184916 120 13 -0.7436823498645744 120 14 -4.381966916136207 120 15 -0.9282099909838024 120 16 -5.42914652218095 120 17 -1.1684308472173406 120 18 -2.6414539998143773 120 19 -0.02867865358475541 120 20 -0.5104895777182712 120 21 3.013582726958326 120 22 4.592576703260818 120 23 4.592155274488682 120 24 3.219140628056479 120 25 3.9716461165360712 120 26 5.559806577563844 120 27 4.477085005013112 120 28 3.9083074305109307 120 29 4.865218057791221 120 30 4.561569530657376 120 31 3.0828152736589196 120 32 3.3447559670419054 120 33 3.017358249108245 120 34 2.9245027067528753 120 35 2.617321465698339 120 36 3.579070938853576 120 37 3.6222524151237465 120 38 4.219307935066592 120 39 4.493317163296279 120 40 5.873278954448306 120 41 -7.588337765493504 120 42 -5.236408155838909 120 43 -8.762389712209593 120 44 -14.72766497801706 120 45 -6.34466505965953 120 46 -9.988055470428039 120 47 -9.193115728723212 120 48 -6.18298590025773 120 49 -14.731440304981135 120 50 -4.295445212158661 120 51 -1.5912062358916899 120 52 -1.5303108610451908 120 53 -1.160876577008338 120 54 -1.234632134764766 120 55 -0.5198237187831154 120 56 2.594719313734913 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1181.0 5 21.0 6 41.0 7 65.0 8 93.0 9 78.0 10 79.0 11 83.0 12 83.0 13 63.0 14 87.0 15 115.0 16 152.0 17 204.0 18 242.0 19 324.0 20 491.0 21 570.0 22 683.0 23 838.0 24 943.0 25 1055.0 26 1171.0 27 1254.0 28 1166.0 29 1302.0 30 1406.0 31 1645.0 32 1817.0 33 1981.0 34 2294.0 35 2479.0 36 2684.0 37 2946.0 38 2992.0 39 2809.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.47512486948669 0.39788921184073145 0.10441064424189406 0.0225752744306798 8 99.37635804385248 0.35273866297937184 0.1552050117109236 0.11569828145723397 9 97.63806191269012 0.8465727911504924 1.0807912633687955 0.4345740327905861 10 40.88946581256878 28.295284589553294 23.593983689364222 7.2212659085137005 11 27.75629991252081 21.429579253322796 19.347010187092586 31.4671106470638 12 25.66244320907526 29.398651127352764 19.759008945452493 25.179896718119483 13 21.830290374467364 13.339165279227927 21.98549538617829 42.84504896012642 14 21.102237774077942 36.831560233654095 21.36185343003076 20.70434856223721 15 39.60595322466336 17.678242381289866 21.59036144578313 21.125442948263643 16 25.903444381705253 34.856013551665725 20.85827216261999 18.382269904009036 17 25.35843305486566 18.443779634228946 38.59787762474599 17.599909686159403 18 20.96678612749386 17.55509777915738 22.77562999125208 38.70248610209668 19 22.567258560822065 18.934025915365723 21.031533184654037 37.46718233915817 20 22.635965531854783 20.62720723265998 36.765079813532985 19.971747421952255 21 22.395304210407495 17.57534710463935 24.599277570831923 35.43007111412123 22 19.37805118943477 17.732878065298983 40.872534356745774 22.01653638852047 23 18.359341930750343 20.73821147388323 37.86437903885769 23.038067556508736 24 19.21426958681418 20.927410250620905 23.935990065477533 35.92233009708738 25 22.745872724707212 22.401580358402708 35.72738817553267 19.125158741357414 26 39.85036702428007 21.57538114059853 18.96668548842462 19.607566346696782 27 20.560246237257505 36.4527151046226 19.961596024058963 23.025442634060937 28 28.70043742062932 30.18484549174545 20.135459291660787 20.979257795964443 29 33.06014017635089 28.493104227899618 19.494686864119377 18.952068731630114 30 30.052515669998307 20.715455418148963 29.981930092043598 19.250098819809136 31 30.362384285391737 29.343531271167304 21.807970196432603 18.486114247008352 32 32.34837580786273 27.353032483842743 21.942821663421107 18.355770044873424 33 31.19774011299435 28.039548022598872 21.7090395480226 19.053672316384183 34 22.824520249837494 22.044484639516153 38.3008789531696 16.830116157476755 35 20.643757418187985 22.056745605606736 39.86887469620754 17.43062227999774 36 28.173348390739694 23.195934500282327 29.638622247317898 18.99209486166008 37 29.304401876024183 20.362208283889924 31.34147030570153 18.99191953438436 38 32.3531906471769 22.28787921626283 27.408182306539626 17.95074783002064 39 29.306544591386913 22.586752571493165 21.264270374138125 26.8424324629818 40 24.71565059144677 17.999317561419474 28.130686988171067 29.154344858962695 41 20.764015496809478 15.904170464904285 26.042616226071104 37.28919781221513 42 21.945976523829728 29.744024890397398 21.94880497807948 26.361193607693394 43 17.98636453647911 26.3996152649297 29.81413901383349 25.7998811847577 44 19.475920679886684 27.453257790368273 28.226628895184135 24.84419263456091 45 27.59780642243329 21.559814563545906 29.760289461781998 21.082089552238806 46 18.52574985851726 30.04244482173175 23.43520090548953 27.996604414261462 47 16.977548474883775 26.82560380995578 22.930604376913482 33.266243338246966 48 26.522659889877172 23.944656219116194 22.803896653960187 26.728787237046447 49 18.034734400633592 28.520110878542738 22.69050178197658 30.754652938847087 50 20.58457711442786 29.5822026232474 23.47071460877431 26.36250565355043 51 19.214876033057852 28.755802105739843 25.914185440960036 26.115136420242273 52 19.497033060186496 35.72478101158519 25.36592257699915 19.41226335122916 53 19.120723777212326 33.36726039016115 25.581000848176423 21.931014984450098 54 19.65906199643796 23.633845023040173 30.260368077347128 26.44672490317474 55 18.244045935396276 23.380664139842732 31.905866380041864 26.469423544719128 56 19.4361178666365 22.17917538600758 32.237995588484814 26.146711158871106 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 1.5 11 2.0 12 3.5 13 10.5 14 16.0 15 29.0 16 42.0 17 48.5 18 55.0 19 59.5 20 64.0 21 69.0 22 85.0 23 96.0 24 106.5 25 117.0 26 106.5 27 96.0 28 115.0 29 156.0 30 178.0 31 185.0 32 192.0 33 217.5 34 243.0 35 267.0 36 291.0 37 332.0 38 442.0 39 511.0 40 552.5 41 594.0 42 624.5 43 655.0 44 779.5 45 904.0 46 994.5 47 1454.0 48 1823.0 49 2616.5 50 3410.0 51 3614.5 52 3819.0 53 3142.0 54 2313.0 55 2161.0 56 2155.5 57 2150.0 58 2235.0 59 2320.0 60 2146.5 61 1973.0 62 1962.0 63 1901.0 64 1851.0 65 1684.0 66 1517.0 67 1360.5 68 1204.0 69 1087.5 70 971.0 71 820.5 72 586.5 73 503.0 74 424.0 75 345.0 76 294.5 77 244.0 78 169.0 79 81.0 80 68.0 81 60.0 82 52.0 83 72.0 84 92.0 85 57.0 86 22.0 87 13.0 88 2.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.4571493072212659 16 0.04797245816519457 17 0.014109546519174874 18 0.0 19 0.03950673025368964 20 0.11852019076106894 21 0.002821909303834975 22 0.0 23 0.0 24 0.014109546519174874 25 0.00564381860766995 26 0.04797245816519457 27 0.0677258232920394 28 0.00564381860766995 29 0.14956119310325366 30 0.05361627677286451 31 0.014109546519174874 32 0.0112876372153399 33 0.10441064424189406 34 0.15238310240708863 35 0.14391737449558373 36 0.04797245816519457 37 0.12134210006490391 38 0.19188983266077828 39 0.13827355588791376 40 0.7590936027316082 41 0.9396957981770466 42 0.23139656291446795 43 0.24832801873747776 44 0.3866015746253915 45 0.17213646753393347 46 0.27372520247199256 47 0.4543273979174309 48 0.06208200468436944 49 0.2342184722183029 50 0.17213646753393347 51 0.29630047690267236 52 0.13262973728024383 53 0.1890679233569433 54 0.1806021954454384 55 0.2342184722183029 56 0.2116431977876231 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 35437.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.53435674577419 #Duplication Level Percentage of deduplicated Percentage of total 1 89.27404295051355 64.75435279510117 2 6.380329909741675 9.255862516578716 3 1.8479614067849364 4.021220757964839 4 0.7625272331154684 2.21237689420662 5 0.451291627762216 1.6367073962242855 6 0.24509803921568626 1.0666817168496205 7 0.18674136321195145 0.9481615260885515 8 0.12449424214130098 0.7224087817817536 9 0.1206037970743853 0.7873126957699579 >10 0.5291005291005291 6.549651494200977 >50 0.054466230936819175 2.3873352710443885 >100 0.015561780267662622 1.8765696870502582 >500 0.007780890133831311 3.7813584671388663 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGCGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 725 2.0458842452803565 No Hit GGGCGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 615 1.7354742218585093 No Hit GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 235 0.663148686401219 No Hit GGGCGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 192 0.5418065863363152 No Hit GGGCGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 130 0.36684820949854674 No Hit GGGCGAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 108 0.30476620481417727 No Hit GGGCGAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 75 0.2116431977876231 No Hit GGGCGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC 72 0.20317746987611818 No Hit GGGCGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 71 0.20035556057228318 No Hit GGGCGAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT 64 0.1806021954454384 No Hit GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 64 0.1806021954454384 No Hit GGGCGAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG 63 0.1777802861416034 No Hit GGGCGAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 62 0.17495837683776844 No Hit GGGCGAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 56 0.15802692101475857 No Hit GGGCGAGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT 56 0.15802692101475857 No Hit GGGCGAGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC 54 0.15238310240708863 No Hit GGGCGAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC 53 0.14956119310325366 No Hit GGGCGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC 53 0.14956119310325366 No Hit GGGCGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT 52 0.1467392837994187 No Hit GGGCGAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 51 0.14391737449558373 No Hit GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 48 0.1354516465840788 No Hit GGGCGAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG 44 0.12416400936873888 No Hit GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 43 0.12134210006490391 No Hit GGGCGAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 42 0.11852019076106894 No Hit GGGCGAGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT 41 0.11569828145723397 No Hit GGGCGAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC 37 0.10441064424189406 No Hit GGGCGAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 37 0.10441064424189406 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.014109546519174874 0.0 0.0 0.0 0.0 7 0.014109546519174874 0.0 0.0 0.0 0.0 8 0.014109546519174874 0.0 0.0 0.0 0.0 9 0.014109546519174874 0.0 0.0 0.0 0.0 10 0.014109546519174874 0.0 0.0 0.0 0.0 11 0.019753365126844825 0.0 0.0 0.0 0.0 12 0.025397183734514772 0.0 0.0 0.0 0.0 13 0.025397183734514772 0.0 0.0 0.0 0.0 14 0.05361627677286452 0.0 0.0 0.0 0.0 15 0.05361627677286452 0.0 0.0 0.0 0.0 16 0.05926009538053447 0.0 0.0 0.0 0.0 17 0.06490391398820441 0.0 0.0 0.0 0.0 18 0.06490391398820441 0.0 0.0 0.0 0.0 19 0.07336964189970935 0.0 0.0 0.0 0.0 20 0.07619155120354432 0.0 0.0 0.0 0.0 21 0.08183536981121427 0.0 0.0 0.0 0.0 22 0.08465727911504924 0.0 0.0 0.0 0.0 23 0.08747918841888422 0.0 0.0 0.0 0.0 24 0.08747918841888422 0.0 0.0 0.0 0.0 25 0.08747918841888422 0.0 0.0 0.0 0.0 26 0.08747918841888422 0.0 0.0 0.0 0.0 27 0.11005446284956401 0.0 0.0 0.0 0.0 28 0.12134210006490391 0.0 0.0 0.0 0.0 29 0.12134210006490391 0.0 0.0 0.0 0.0 30 0.12416400936873889 0.0 0.0 0.0 0.0 31 0.14956119310325366 0.0 0.0 0.0 0.0 32 0.15238310240708863 0.0 0.0 0.0 0.0 33 0.16084883031859357 0.0 0.0 0.0 0.0 34 0.16367073962242853 0.0 0.0 0.0 0.0 35 0.16367073962242853 0.0 0.0 0.0 0.0 36 0.16367073962242853 0.0 0.0 0.0 0.0 37 0.16367073962242853 0.0 0.0 0.0 0.0 38 0.16367073962242853 0.0 0.0 0.0 0.0 39 0.1664926489262635 0.0 0.0 0.0 0.0 40 0.16931455823009847 0.0 0.0 0.0 0.0 41 0.16931455823009847 0.0 0.0 0.0 0.0 42 0.18342410474927337 0.0 0.0 0.0 0.0 43 0.19188983266077828 0.0 0.0 0.0 0.0 44 0.20317746987611818 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTCCA 45 1.1823431E-10 50.42264 37 CTCAATC 25 1.9463938E-5 50.422638 40 TGTTCCC 20 3.960011E-4 50.422634 38 GTACAAA 15 0.008228041 50.20685 45 GACCAAA 15 0.008228041 50.20685 45 GTACAAG 15 0.00827463 50.135326 34 CCATCCG 15 0.00827463 50.135326 42 CTCCGCC 25 2.0135532E-5 50.135326 44 ACTGAAC 20 4.07358E-4 50.135323 42 CAGAACC 15 0.008321415 50.06401 13 GTCCCAG 15 0.008368396 49.9929 9 GAACTGT 35 5.1013558E-8 49.9929 9 GATGGTT 15 0.008368396 49.9929 9 CCTGTAC 15 0.008415574 49.92199 31 CGGGTAA 15 0.008415574 49.92199 31 AGCGGGT 15 0.008415574 49.92199 29 CAGCGGG 15 0.008415574 49.92199 28 CTGTACA 15 0.008415574 49.92199 32 GGGCCCT 15 0.008415574 49.92199 27 GCGGGTA 15 0.008415574 49.92199 30 >>END_MODULE