FastQCFastQC Report
Fri 17 Jun 2016
SRR1526033_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526033_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5746
Sequences flagged as poor quality0
Sequence length40
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT180.3132613992342499No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT170.2958579881656805No Hit
GGGCGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT170.2958579881656805No Hit
GGGCGAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC150.2610511660285416No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG110.19143752175426382No Hit
GGGCGAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT90.15663069961712495No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG90.15663069961712495No Hit
GGGCGAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC90.15663069961712495No Hit
GGGCGAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.15663069961712495No Hit
GGGCGAGGGGAGTGCAGTGCTGGGCGAGGGACACGCAGGG80.13922728854855554No Hit
GGGCGAGGGATGAGATGGACTTAAATGAATTGACGGGTAC80.13922728854855554No Hit
GGGCGAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT80.13922728854855554No Hit
GGGCGAGGGACACGCAGGGATGAGATGGGCAAAAAAAAAA70.12182387747998608No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC70.12182387747998608No Hit
GGGCGAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC70.12182387747998608No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACC70.12182387747998608No Hit
GGGCGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT60.10442046641141664No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG60.10442046641141664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGAG251.8226463E-433.8859676
GAGGGGA502.8921932E-1033.8859675
GAGGGAG552.0008883E-1133.8859675
AGGGGGG601.8189894E-1233.8859636
AGGGGGC200.002606284833.8859636
AGGGGCT200.002606284833.8859636
CGAGGGT454.2145984E-933.8859634
AGGGAGT358.8370325E-733.8859636
GAGGGTG200.002606284833.8859635
GAGGGCA200.002606284833.8859635
CGAGGGG2200.032.3456954
GAGGGGG1100.032.3456955
GGGCGAG6100.031.6639331
GAGGGAA750.031.6269025
GGCGAGG5950.031.6079182
GCGAGGG5750.031.528683
CGAGGGA2450.031.1197624
CGAGGGC605.4569682E-1131.0621344
AGGGAAG402.5098961E-629.6502196
GAGGGAT750.029.3678365
Produced by FastQC (version 0.11.5)