##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526033_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5746
Sequences flagged as poor quality	0
Sequence length	40
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.04559693699965	33.0	29.0	36.0	19.0	38.0
2	27.678384963452835	31.0	24.0	35.0	4.0	38.0
3	23.819178558997564	26.0	17.0	33.0	4.0	36.0
4	20.608075182735817	19.0	10.0	33.0	4.0	38.0
5	25.883223111729897	29.0	19.0	35.0	4.0	38.0
6	25.810650887573964	30.0	19.0	34.0	4.0	37.0
7	25.471980508179602	29.0	19.0	34.0	4.0	37.0
8	26.302993386703793	31.0	20.0	35.0	4.0	37.0
9	25.302645318482423	30.0	18.0	34.0	4.0	37.0
10	22.346327880264532	25.0	14.0	31.0	4.0	35.0
11	23.727114514444832	27.0	14.0	33.0	4.0	37.0
12	23.976853463278804	28.0	14.0	34.0	4.0	37.0
13	24.833275321963104	29.0	17.0	35.0	4.0	37.0
14	25.243299686738602	30.0	18.0	35.0	4.0	38.0
15	23.806648103028195	28.0	14.0	35.0	4.0	37.0
16	22.193351896971805	26.0	10.0	33.0	4.0	36.0
17	22.811347024016708	27.0	10.0	33.0	4.0	36.0
18	22.57152801949182	27.0	7.0	33.0	4.0	36.0
19	19.425513400626524	20.0	4.0	31.0	4.0	35.0
20	21.423076923076923	26.0	4.0	33.0	4.0	36.0
21	19.20048729550992	20.0	4.0	31.0	4.0	35.0
22	18.88966237382527	20.0	4.0	31.0	4.0	35.0
23	19.075704838148276	21.0	4.0	31.0	4.0	35.0
24	18.73442394709363	20.0	4.0	31.0	4.0	35.0
25	18.092238078663417	19.0	4.0	31.0	4.0	35.0
26	16.18952314653672	12.0	4.0	30.0	4.0	35.0
27	16.05012182387748	11.0	4.0	29.0	4.0	35.0
28	15.69074138531152	9.0	4.0	29.0	4.0	35.0
29	15.1240863209189	4.0	4.0	29.0	4.0	35.0
30	14.47859380438566	4.0	4.0	27.0	4.0	34.0
31	14.097981204316046	4.0	4.0	28.0	4.0	34.0
32	13.446397493908806	4.0	4.0	27.0	4.0	33.0
33	12.860598677340759	4.0	4.0	25.0	4.0	32.0
34	12.453532892446919	4.0	4.0	24.0	4.0	32.0
35	11.744517925513401	4.0	4.0	22.0	4.0	31.0
36	11.609467455621301	4.0	4.0	18.0	4.0	33.0
37	11.453532892446919	4.0	4.0	18.0	4.0	33.0
38	11.152105812739297	4.0	4.0	17.0	4.0	32.0
39	10.669161155586496	4.0	4.0	17.0	4.0	32.0
40	10.411590671771668	4.0	4.0	16.0	4.0	32.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-4.307755047982322
1	2	-0.6461298549934824
1	3	-4.344344544344548
1	4	8.6813011735739
1	5	5.734363414363415
1	6	5.162147902602449
1	7	2.7530930937749147
1	8	-0.8752091343000288
1	9	-5.723989596262317
1	10	3.358783791965603
1	11	-3.294452290134114
1	12	1.1277828989192678
1	13	-3.450564183291462
1	14	3.2074476785840496
1	15	0.27974031019486034
1	16	-4.873642191142185
1	17	-1.8100829221283732
1	18	-3.2416016306925393
1	19	-6.037408071726251
1	20	1.4522949777495242
1	21	-1.6231271253998507
1	22	0.9981392849574675
1	23	0.6866768332677395
1	24	-2.2331562881562963
1	25	1.000831491740584
1	26	-2.770607069697977
1	27	-0.7062940847031705
1	28	-2.8205803792167377
1	29	-3.378836693609415
1	30	-4.868465700965695
1	31	2.185508658008658
1	32	0.24081862581862445
1	33	-1.3214430518975977
1	34	1.3721958849231584
1	35	1.8710805608532883
1	36	1.0883713508713484
1	37	0.2579637786455926
1	38	3.3293228993228965
1	39	1.9987660571751427
1	40	1.2725703084793967
2	1	-4.307755047982322
2	2	-3.0627965216601503
2	3	-6.92767787767788
2	4	-11.402032159759433
2	5	-3.682303252303253
2	6	-17.83785209739755
2	7	-15.580240239558417
2	8	3.2081241990333034
2	9	-0.6406562629289851
2	10	-3.224549541367729
2	11	-2.294452290134114
2	12	-7.205550434414064
2	13	-7.36723084995813
2	14	6.540781011917382
2	15	6.1130736435281925
2	16	-1.873642191142185
2	17	4.689917077871627
2	18	-2.0749349640258714
2	19	-9.53740807172625
2	20	11.202294977749524
2	21	10.710206207933481
2	22	12.0814726182908
2	23	-3.0633231667322605
2	24	1.3501770451770376
2	25	5.834164825073916
2	26	9.229392930302023
2	27	11.960372581963496
2	28	7.012752954116596
2	29	18.20449663972392
2	30	9.048200965700971
2	31	2.852175324675324
2	32	12.65748529248529
2	33	19.928556948102404
2	34	4.122195884923158
2	35	-0.8789194391467117
2	36	-1.2449619824619855
2	37	4.5912971119789265
2	38	5.16265623265623
2	39	2.9987660571751427
2	40	1.5225703084793967
3	1	-1.3077550479823223
3	2	2.9372034783398497
3	3	3.57232212232212
3	4	-9.902032159759433
3	5	-1.182303252303253
3	6	-4.337852097397551
3	7	2.4197597604415826
3	8	0.7081241990333034
3	9	0.35934373707101486
3	10	-2.224549541367729
3	11	10.205547709865886
3	12	6.794449565585936
3	13	-12.36723084995813
3	14	5.040781011917382
3	15	4.6130736435281925
3	16	12.126357808857815
3	17	9.189917077871627
3	18	13.925065035974129
3	19	-7.037408071726251
3	20	15.702294977749524
3	21	15.210206207933481
3	22	-7.4185273817092
3	23	13.43667683326774
3	24	13.350177045177038
3	25	-13.165835174926084
3	26	-11.770607069697977
3	27	-11.539627418036504
3	28	-11.487247045883404
3	29	-10.795503360276081
3	30	-9.951799034299029
3	31	-9.647824675324676
3	32	-9.34251470751471
3	33	-8.571443051897598
3	34	-7.877804115076842
3	35	-7.378919439146712
3	36	-7.7449619824619855
3	37	-7.4087028880210735
3	38	-6.83734376734377
3	39	-6.001233942824857
3	40	-5.977429691520603
5	1	-3.4327550479823223
5	2	-5.18779652166015
5	3	-2.92767787767788
5	4	-11.277032159759433
5	5	-1.557303252303253
5	6	-8.587852097397551
5	7	-15.830240239558417
5	8	-16.791875800966697
5	9	-18.515656262928985
5	10	-11.974549541367729
5	11	-10.294452290134114
5	12	-11.705550434414064
5	13	-15.24223084995813
5	14	-14.209218988082618
5	15	-12.636926356471808
5	16	-12.248642191142185
5	17	-8.810082922128373
5	18	-9.449934964025871
5	19	-6.162408071726251
5	20	-8.547705022250476
5	21	-6.039793792066519
5	22	-4.9185273817092
5	23	-7.3133231667322605
5	24	-5.774822954822962
5	25	-4.540835174926084
5	26	-0.7706070696979772
5	27	-0.9146274180365044
5	28	-3.2372470458834037
5	29	-2.795503360276081
5	30	-1.7017990342990288
5	31	-0.6478246753246761
5	32	0.28248529248529053
5	33	-1.9464430518975977
5	34	1.2471958849231584
5	35	-2.1289194391467117
5	36	-0.8699619824619855
5	37	0.5912971119789265
5	38	4.41265623265623
5	39	0.24876605717514266
5	40	0.2725703084793967
6	1	0.6922449520176777
6	2	1.0483145894509605
6	3	-0.6499000999001012
6	4	1.7646345069072353
6	5	5.5954745254745255
6	6	-0.11562987517532974
6	7	-7.91357357289175
6	8	-6.9585424676333645
6	9	-4.307322929595653
6	10	0.10878379196560317
6	11	5.983325487643665
6	12	6.2388940100303785
6	13	5.5216580389307595
6	14	3.1518921230284924
6	15	8.27974031019486
6	16	1.126357808857815
6	17	4.078805966760516
6	18	4.3695094804185715
6	19	4.407036372718192
6	20	0.14673942219396707
6	21	5.543539541266814
6	22	1.2481392849574675
6	23	2.1033434999344074
6	24	2.9057326007325948
6	25	2.6119426028516948
6	26	4.007170708079801
6	27	5.1270392486301635
6	28	5.957197398561039
6	29	5.42671886194614
6	30	6.492645410145414
6	31	6.241064213564213
6	32	5.324151959151958
6	33	3.9841125036579594
6	34	5.4555292182564905
6	35	2.2877472275199544
6	36	3.477260239760236
6	37	2.813519334201148
6	38	5.384878454878452
6	39	4.998766057175143
6	40	1.911459197368286
7	1	4.601335861108584
7	2	6.4826580237943965
7	3	5.9359584859584835
7	4	14.916149658422388
7	5	9.363151293151294
7	6	11.480329720784269
7	7	0.601577942259766
7	8	-0.7464212555121499
7	9	2.1775255552528314
7	10	6.502723185904998
7	11	1.2055477098658862
7	12	2.612631383767752
7	13	5.814587331860054
7	14	1.0407810119173817
7	15	6.158528188982739
7	16	4.489994172494178
7	17	5.189917077871627
7	18	0.47051958142867534
7	19	5.144410110091933
7	20	6.0659313413858875
7	21	5.1192971170243915
7	22	3.854199891018073
7	23	1.709404105995013
7	24	5.622904317904311
7	25	7.925073915983006
7	26	4.411211112120206
7	27	6.551281672872586
7	28	4.9672984086620495
7	29	3.9317693669966456
7	30	3.9572918747918813
7	31	-1.1023701298701312
7	32	1.3847580197580172
7	33	-1.2987157791703243
7	34	0.849468612195885
7	35	-0.28801034823761995
7	36	1.8004925629925594
7	37	0.22766074834256322
7	38	-1.0191619491619512
7	39	-1.4557793973703124
7	40	1.29529758120667
8	1	4.692244952017678
8	2	6.43720347833985
8	3	5.57232212232212
8	4	2.2979678402405668
8	5	3.5176967476967462
8	6	6.76214790260245
8	7	4.419759760441583
8	8	0.7081241990333034
8	9	2.059343737071014
8	10	-1.724549541367729
8	11	-1.6944522901341124
8	12	1.0944495655859363
8	13	1.8327691500418695
8	14	-1.4592189880826183
8	15	-4.786926356471806
8	16	2.9263578088578157
8	17	-4.810082922128373
8	18	0.52506503597413
8	19	3.5625919282737506
8	20	1.9022949777495235
8	21	1.5102062079334821
8	22	0.28147261829079895
8	23	4.036676833267741
8	24	4.450177045177039
8	25	-9.365835174926083
8	26	-5.370607069697977
8	27	-8.239627418036505
8	28	-4.387247045883404
8	29	-7.795503360276081
8	30	-5.851799034299029
8	31	-5.747824675324676
8	32	-5.442514707514709
8	33	-4.271443051897597
8	34	-5.577804115076842
8	35	-5.6789194391467115
8	36	-5.044961982461985
8	37	-4.708702888021073
8	38	-4.137343767343769
8	39	-3.301233942824857
8	40	-3.277429691520603
9	1	-0.20775504798232092
9	2	0.23720347833985045
9	3	-2.027677877677881
9	4	-3.302032159759431
9	5	4.017696747696746
9	6	-2.9378520973975526
9	7	5.419759760441583
9	8	4.508124199033304
9	9	3.859343737071015
9	10	4.275450458632271
9	11	4.9055477098658855
9	12	5.794449565585936
9	13	3.5327691500418688
9	14	1.0407810119173817
9	15	2.1130736435281925
9	16	3.2263578088578164
9	17	5.389917077871626
9	18	7.425065035974129
9	19	4.1625919282737485
9	20	9.102294977749523
9	21	7.810206207933483
9	22	9.981472618290798
9	23	10.73667683326774
9	24	9.050177045177037
9	25	9.334164825073916
9	26	0.3293929303020242
9	27	2.360372581963494
9	28	2.6127529541165977
9	29	-1.795503360276081
9	30	4.148200965700973
9	31	0.7521753246753242
9	32	5.6574852924852905
9	33	3.1285569481024016
9	34	3.5221958849231587
9	35	0.5210805608532887
9	36	6.555038017538015
9	37	8.691297111978928
9	38	6.962656232656231
9	39	6.198766057175142
9	40	5.222570308479396
10	1	7.620816380589108
10	2	8.50863204976842
10	3	11.786607836607836
10	4	13.883682125954852
10	5	11.103411033411032
10	6	10.733576474031018
10	7	6.77690261758444
10	8	8.922409913319019
10	9	8.57362945135673
10	10	9.418307601489413
10	11	6.634119138437313
10	12	10.794449565585936
10	13	8.418483435756155
10	14	8.397923869060236
10	15	9.327359357813908
10	16	12.34064352314353
10	17	13.832774220728773
10	18	12.639350750259844
10	19	15.89116335684518
10	20	12.202294977749524
10	21	16.353063350790627
10	22	17.938615475433654
10	23	17.150962547553455
10	24	14.778748473748468
10	25	19.19130768221677
10	26	10.729392930302023
10	27	7.74608686767778
10	28	10.012752954116596
10	29	9.990210925438204
10	30	7.2624866799866865
10	31	1.923603896103895
10	32	1.371771006771004
10	33	1.9999855195309735
10	34	3.336481599208872
10	35	2.549651989424717
10	36	0.469323731823728
10	37	-1.7658457451639311
10	38	1.5912276612276592
10	39	4.284480342889429
10	40	2.165427451336539
11	1	-4.418866159093433
11	2	-5.9516854105490395
11	3	-1.983233433233437
11	4	-8.235365493092766
11	5	-0.7378588078588102
11	6	2.1065923470468917
11	7	0.7530930937749147
11	8	2.485901976811082
11	9	0.692677070404347
11	10	-1.1134384302566183
11	11	-0.5722300679118923
11	12	0.9055606766970463
11	13	1.077213594486313
11	14	-1.070330099193729
11	15	-1.4980374675829182
11	16	-0.09586441336440643
11	17	2.8565837445382947
11	18	2.702842813751907
11	19	5.184814150495971
11	20	4.146739422193967
11	21	8.654650652377924
11	22	6.69258372940191
11	23	6.658899055489961
11	24	5.7946214896214805
11	25	9.500831491740584
11	26	3.118281819190912
11	27	0.3492614708523849
11	28	-0.2650248236611823
11	29	-3.0177255824983025
11	30	-2.3962434787434734
11	31	-1.4256024531024547
11	32	-2.45362581862582
11	33	-0.12699860745315306
11	34	0.23330699603426908
11	35	0.6210805608532883
11	36	-3.8560730935730962
11	37	-3.853147332465518
11	38	-3.6151215451215473
11	39	-0.001233942824857337
11	40	-3.5329852470761587
12	1	1.615321875094601
12	2	4.47566501680139
12	3	4.418475968475967
12	4	-5.2866475443748175
12	5	2.586927516927517
12	6	6.815994056448602
12	7	-1.1956248549430342
12	8	4.631201122110227
12	9	-0.8714254936982151
12	10	6.390835074016888
12	11	7.436316940635116
12	12	2.6406034117397823
12	13	6.709692226964947
12	14	8.425396396532769
12	15	5.074612105066652
12	16	8.818665501165508
12	17	11.651455539410087
12	18	8.848141959051052
12	19	8.885668851350673
12	20	11.240756516211064
12	21	12.517898515625788
12	22	13.504549541367723
12	23	10.43667683326774
12	24	13.888638583638578
12	25	8.98801097892007
12	26	4.998623699532793
12	27	9.691141812732726
12	28	7.43582987719352
12	29	2.666035101262379
12	30	4.2020471195471245
12	31	2.352175324675324
12	32	3.1190237540237504
12	33	0.9670184865639406
12	34	1.199118961846235
12	35	3.1595420993148267
12	36	-1.9757312132312155
12	37	1.0528355735173882
12	38	-0.6834976134976163
12	39	0.3833814417905277
12	40	2.17641646232555
13	1	-6.057755047982322
13	2	-3.0627965216601503
13	3	-1.1776778776778798
13	4	3.0979678402405675
13	5	3.067696747696747
13	6	1.9121479026024488
13	7	-6.830240239558417
13	8	-0.5418758009666966
13	9	-1.1406562629289851
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114	2	-14.31279652166015
114	3	-8.92767787767788
114	4	-11.902032159759433
114	5	-14.932303252303253
114	6	-11.587852097397551
114	7	-11.330240239558417
114	8	-8.291875800966697
114	9	-11.390656262928985
114	10	-4.974549541367729
114	11	-6.044452290134114
114	12	-3.7055504344140644
114	13	-8.11723084995813
114	14	-11.709218988082618
114	15	-6.8869263564718075
114	16	-10.373642191142185
114	17	-10.810082922128373
114	18	-9.074934964025871
114	19	-9.53740807172625
114	20	-9.047705022250476
114	21	-6.539793792066519
114	22	-6.1685273817092
114	23	-7.8133231667322605
114	24	-11.149822954822962
114	25	-7.165835174926084
114	26	-10.020607069697977
114	27	-4.289627418036504
114	28	-4.237247045883404
114	29	-3.545503360276081
114	30	-6.701799034299029
114	31	-8.147824675324676
114	32	-6.0925147075147095
114	33	-7.071443051897598
114	34	-2.6278041150768416
114	35	-3.8789194391467117
114	36	-1.9949619824619855
114	37	-3.6587028880210735
114	38	0.16265623265623042
114	39	-3.0012339428248573
114	40	0.5225703084793967
117	1	0.6922449520176777
117	2	2.4372034783398497
117	3	7.57232212232212
117	4	-7.9020321597594325
117	5	3.817696747696747
117	6	-3.837852097397551
117	7	4.919759760441583
117	8	-9.791875800966697
117	9	-5.140656262928985
117	10	0.275450458632271
117	11	-4.294452290134114
117	12	-2.2055504344140644
117	13	-2.86723084995813
117	14	2.5407810119173817
117	15	3.6130736435281925
117	16	1.126357808857815
117	17	11.189917077871627
117	18	8.425065035974129
117	19	7.962591928273749
117	20	8.702294977749524
117	21	14.710206207933481
117	22	15.0814726182908
117	23	11.93667683326774
117	24	15.350177045177038
117	25	13.334164825073916
117	26	19.229392930302023
117	27	19.460372581963497
117	28	23.012752954116596
117	29	22.20449663972392
117	30	22.54820096570097
117	31	10.352175324675324
117	32	20.15748529248529
117	33	3.4285569481024023
117	34	20.12219588492316
117	35	12.121080560853288
117	36	9.255038017538014
117	37	-0.9087028880210735
117	38	7.16265623265623
117	39	0.49876605717514266
117	40	0.5225703084793967
118	1	-3.5577550479823223
118	2	1.4372034783398497
118	3	3.07232212232212
118	4	-11.652032159759433
118	5	-6.682303252303253
118	6	-10.087852097397551
118	7	-13.080240239558417
118	8	-5.291875800966697
118	9	-12.640656262928985
118	10	-5.724549541367729
118	11	-0.04445229013411378
118	12	-3.7055504344140644
118	13	-9.11723084995813
118	14	-8.709218988082618
118	15	-6.6369263564718075
118	16	1.376357808857815
118	17	-0.060082922128373184
118	18	-9.074934964025871
118	19	-7.037408071726251
118	20	-12.297705022250476
118	21	-6.789793792066519
118	22	-7.1685273817092
118	23	-6.3133231667322605
118	24	-9.399822954822962
118	25	-6.165835174926084
118	26	-10.270607069697977
118	27	-7.289627418036504
118	28	-4.237247045883404
118	29	-10.795503360276081
118	30	-9.951799034299029
118	31	-9.647824675324676
118	32	-9.34251470751471
118	33	-8.571443051897598
118	34	-7.877804115076842
118	35	-7.378919439146712
118	36	-7.7449619824619855
118	37	-7.4087028880210735
118	38	-6.83734376734377
118	39	-6.001233942824857
118	40	-5.977429691520603
119	1	-25.307755047982322
119	2	-23.06279652166015
119	3	-19.42767787767788
119	4	-15.902032159759433
119	5	-20.182303252303253
119	6	-20.33785209739755
119	7	-20.580240239558417
119	8	-21.291875800966697
119	9	-20.640656262928985
119	10	-17.22454954136773
119	11	-18.794452290134114
119	12	-19.205550434414064
119	13	-19.36723084995813
119	14	-20.95921898808262
119	15	-19.386926356471808
119	16	-16.873642191142185
119	17	-17.810082922128373
119	18	-17.07493496402587
119	19	-14.03740807172625
119	20	-16.297705022250476
119	21	-13.789793792066519
119	22	-13.4185273817092
119	23	-13.56332316673226
119	24	-13.649822954822962
119	25	-13.165835174926084
119	26	-11.770607069697977
119	27	-11.539627418036504
119	28	-11.487247045883404
119	29	-10.795503360276081
119	30	-9.951799034299029
119	31	-9.647824675324676
119	32	-9.34251470751471
119	33	-8.571443051897598
119	34	-7.877804115076842
119	35	-7.378919439146712
119	36	-7.7449619824619855
119	37	-7.4087028880210735
119	38	-6.83734376734377
119	39	-6.001233942824857
119	40	-5.977429691520603
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	581.0
5	51.0
6	46.0
7	61.0
8	54.0
9	78.0
10	88.0
11	116.0
12	154.0
13	159.0
14	187.0
15	220.0
16	188.0
17	209.0
18	223.0
19	248.0
20	283.0
21	282.0
22	316.0
23	325.0
24	277.0
25	308.0
26	279.0
27	260.0
28	228.0
29	181.0
30	121.0
31	93.0
32	57.0
33	44.0
34	22.0
35	3.0
36	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.12785627071341	0.6105006105006106	0.1221001221001221	0.13954299668585382
8	97.85900783289817	0.7832898172323759	0.5395996518711923	0.8181026979982594
9	97.6144872018109	1.5671251958906496	0.5572000696500088	0.2611875326484416
10	37.52175426383571	38.96623738252698	11.434041072050121	12.077967281587192
11	28.489383919248173	29.84684998259659	23.37278106508876	18.29098503306648
12	39.27949878176123	19.19596240863209	21.371388792203273	20.153150017403412
13	28.35509138381201	22.07136640557006	24.595300261096607	24.978241949521323
14	27.6192133658197	26.679429168116954	26.139923424991295	19.56143404107205
15	26.9926905673512	26.522798468499825	24.921684650191438	21.562826313957537
16	26.209537069265576	26.10511660285416	25.635224504002785	22.05012182387748
17	30.943264879916466	26.923076923076923	21.528019491820398	20.605638705186216
18	29.96867386007657	27.11451444483119	21.44100243647755	21.47580925861469
19	22.348617430871542	20.511025551277566	16.748337416870843	40.39201960098005
20	24.62582666202576	32.59658893143056	19.89209885137487	22.885485555168813
21	21.266968325791854	25.565610859728505	29.707622694048037	23.459798120431604
22	23.32057083188305	22.241559345631746	25.983292725374174	28.454577097111034
23	24.034110685694397	29.342151061608075	26.540201879568396	20.08353637312913
24	22.750217580504785	31.67972149695387	23.167972149695387	22.40208877284595
25	22.299651567944252	30.818815331010452	24.581881533101047	22.299651567944252
26	20.290870860346942	26.42369020501139	31.15472227089539	22.130716663746277
27	20.456128133704734	24.14693593314763	32.190111420612816	23.20682451253482
28	17.426880222841227	27.61142061281337	32.62534818941504	22.33635097493036
29	17.01237580617047	26.233222938818194	34.65225727732264	22.102143977688687
30	15.745422842197035	21.342632955536182	40.40104620749782	22.510897994768964
31	14.951151430565249	25.331472435450102	42.812281926029314	16.905094207955337
32	19.93384401114206	20.78690807799443	39.62395543175488	19.655292479108635
33	16.623150565709313	24.73455178416014	41.35770234986945	17.284595300261095
34	13.36581970066133	23.2335537765402	45.440306300034806	17.960320222763663
35	14.514444831186912	24.817264183780022	44.10024364775496	16.56804733727811
36	14.079359554472676	25.75704838148277	43.734772015315	16.428820048729552
37	13.546926693365839	25.335190666898832	44.29740553717569	16.82047710255964
38	11.836379460400348	25.413402959094867	44.38642297650131	18.36379460400348
39	12.774103724329969	26.38357117995127	42.15106160807519	18.691263487643578
40	10.703097807170206	28.071702053602504	43.00382875043508	18.221371388792203
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	1.5
17	2.0
18	2.0
19	3.5
20	5.0
21	12.0
22	19.0
23	19.0
24	38.5
25	58.0
26	86.0
27	114.0
28	114.0
29	139.5
30	165.0
31	170.5
32	176.0
33	176.0
34	192.5
35	209.0
36	211.0
37	213.0
38	213.0
39	227.5
40	242.0
41	298.5
42	355.0
43	355.0
44	348.5
45	342.0
46	354.5
47	367.0
48	367.0
49	378.0
50	389.0
51	381.0
52	373.0
53	373.0
54	362.0
55	351.0
56	343.0
57	335.0
58	335.0
59	316.0
60	297.0
61	304.5
62	312.0
63	312.0
64	304.5
65	297.0
66	306.0
67	315.0
68	315.0
69	301.5
70	288.0
71	248.5
72	209.0
73	209.0
74	176.5
75	144.0
76	120.0
77	96.0
78	96.0
79	69.5
80	43.0
81	31.5
82	20.0
83	20.0
84	11.0
85	2.0
86	4.0
87	6.0
88	6.0
89	3.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.22624434389140274
8	0.017403411068569442
9	0.05221023320570832
10	0.0
11	0.0
12	0.0
13	0.017403411068569442
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5569091541942222
20	0.0
21	0.0
22	0.0
23	0.0
24	0.017403411068569442
25	0.10442046641141664
26	0.6787330316742082
27	0.034806822137138885
28	0.034806822137138885
29	0.15663069961712495
30	0.19143752175426382
31	0.24364775495997215
32	0.034806822137138885
33	0.017403411068569442
34	0.0
35	0.0
36	0.0
37	0.05221023320570832
38	0.017403411068569442
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	5746.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.82039679777236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.31842304776346	87.52175426383572
2	3.2600454890068233	5.986773407587887
3	0.7012888551933283	1.9317786286112077
4	0.3032600454890068	1.1138183083884443
5	0.0758150113722517	0.34806822137138876
6	0.03790750568612585	0.20884093282283328
7	0.0758150113722517	0.4872955099199443
8	0.05686125852918878	0.41768186564566656
9	0.0758150113722517	0.6265227984684998
>10	0.09476876421531463	1.3574660633484164
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGCGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	18	0.3132613992342499	No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	17	0.2958579881656805	No Hit
GGGCGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	17	0.2958579881656805	No Hit
GGGCGAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC	15	0.2610511660285416	No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	11	0.19143752175426382	No Hit
GGGCGAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.15663069961712495	No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	9	0.15663069961712495	No Hit
GGGCGAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC	9	0.15663069961712495	No Hit
GGGCGAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.15663069961712495	No Hit
GGGCGAGGGGAGTGCAGTGCTGGGCGAGGGACACGCAGGG	8	0.13922728854855554	No Hit
GGGCGAGGGATGAGATGGACTTAAATGAATTGACGGGTAC	8	0.13922728854855554	No Hit
GGGCGAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	8	0.13922728854855554	No Hit
GGGCGAGGGACACGCAGGGATGAGATGGGCAAAAAAAAAA	7	0.12182387747998608	No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC	7	0.12182387747998608	No Hit
GGGCGAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC	7	0.12182387747998608	No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACC	7	0.12182387747998608	No Hit
GGGCGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	6	0.10442046641141664	No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG	6	0.10442046641141664	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.01740341106856944	0.0	0.0	0.0	0.0
7	0.01740341106856944	0.0	0.0	0.0	0.0
8	0.01740341106856944	0.0	0.0	0.0	0.0
9	0.01740341106856944	0.0	0.0	0.0	0.0
10	0.03480682213713888	0.0	0.0	0.0	0.0
11	0.06961364427427776	0.0	0.0	0.0	0.0
12	0.06961364427427776	0.0	0.0	0.0	0.0
13	0.06961364427427776	0.0	0.0	0.0	0.0
14	0.06961364427427776	0.0	0.0	0.0	0.0
15	0.06961364427427776	0.0	0.0	0.0	0.0
16	0.06961364427427776	0.0	0.0	0.0	0.0
17	0.06961364427427776	0.0	0.0	0.0	0.0
18	0.06961364427427776	0.0	0.0	0.0	0.0
19	0.0870170553428472	0.0	0.0	0.0	0.0
20	0.0870170553428472	0.0	0.0	0.0	0.0
21	0.0870170553428472	0.0	0.0	0.0	0.0
22	0.0870170553428472	0.0	0.0	0.0	0.0
23	0.0870170553428472	0.0	0.0	0.0	0.0
24	0.10442046641141664	0.0	0.0	0.0	0.0
25	0.10442046641141664	0.0	0.0	0.0	0.0
26	0.10442046641141664	0.0	0.0	0.0	0.0
27	0.10442046641141664	0.0	0.0	0.0	0.0
28	0.10442046641141664	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGAG	25	1.8226463E-4	33.885967	6
GAGGGGA	50	2.8921932E-10	33.885967	5
GAGGGAG	55	2.0008883E-11	33.885967	5
AGGGGGG	60	1.8189894E-12	33.885963	6
AGGGGGC	20	0.0026062848	33.885963	6
AGGGGCT	20	0.0026062848	33.885963	6
CGAGGGT	45	4.2145984E-9	33.885963	4
AGGGAGT	35	8.8370325E-7	33.885963	6
GAGGGTG	20	0.0026062848	33.885963	5
GAGGGCA	20	0.0026062848	33.885963	5
CGAGGGG	220	0.0	32.345695	4
GAGGGGG	110	0.0	32.345695	5
GGGCGAG	610	0.0	31.663933	1
GAGGGAA	75	0.0	31.626902	5
GGCGAGG	595	0.0	31.607918	2
GCGAGGG	575	0.0	31.52868	3
CGAGGGA	245	0.0	31.119762	4
CGAGGGC	60	5.4569682E-11	31.062134	4
AGGGAAG	40	2.5098961E-6	29.650219	6
GAGGGAT	75	0.0	29.367836	5
>>END_MODULE