FastQCFastQC Report
Fri 17 Jun 2016
SRR1526031_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526031_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049
Sequences flagged as poor quality0
Sequence length40
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT442.1473889702293802No Hit
GGGCGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG381.8545632015617375No Hit
GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT190.9272816007808687No Hit
GGGCGAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG80.3904343582235237No Hit
GGGCGAGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG80.3904343582235237No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC70.3416300634455832No Hit
GGGCGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.3416300634455832No Hit
GGGCGAGGGTCCCAGATCCTCTTCTGAAGGAGATTGGGCG60.29282576866764276No Hit
GGGCGAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT50.2440214738897023No Hit
GGGCGAGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT50.2440214738897023No Hit
GGGCGAGGGGACGCTACTCAGACTACCCAGATGCTTACAC50.2440214738897023No Hit
GGGCGAGGGTCACAGATCCTCTTCTGAGATGAGGATCGGA40.19521717911176184No Hit
GGGCGAGGGTCACAGCTCCTCTTCTGAAGGAGATTGGGCG40.19521717911176184No Hit
GGGCGAGGGGCTGGGCGAGGGTCACAGATCCTCTTCTGAG40.19521717911176184No Hit
GGGCGAGGGATTCCAAAGCTTTCCAGCATAATTTCTGGCT40.19521717911176184No Hit
GGGCGAGGGGGAGGAAGAAGGAGAGGAATACTAAATTAAA40.19521717911176184No Hit
GGGCGAGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT40.19521717911176184No Hit
GGGCGAGGGTCACAGATACTCTTCTGAAGGAGATTGGGCG40.19521717911176184No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG30.14641288433382138No Hit
GGGCGAGGTCACAGATCCTCTTCTGAGATGAGGATCGGAA30.14641288433382138No Hit
GGGCGAGGGTCACAGCTCCTCTTCTGAGATGAGTTTTTGT30.14641288433382138No Hit
GGGCGAGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT30.14641288433382138No Hit
GGGCGAGGGTGCTGCGTCCTTATGAATGTTTTGACCATTT30.14641288433382138No Hit
GGGCGAGGGATGCCAAAGTGTTCTTAGTGGCTGATACTGG30.14641288433382138No Hit
GGGCGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14641288433382138No Hit
GGGCGAGGGAGCTTTTAACCATTGTAGGCCTAAAAGCAGC30.14641288433382138No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT30.14641288433382138No Hit
GGGCGAGGGATCAATAAATGAAAACTGTTCAAAAATTTTA30.14641288433382138No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG251.3950845E-434.06
ACAGATC200.002177709734.012
CGAGGGG750.034.04
CGAGGGC308.746991E-634.04
CAGATCC251.3950845E-434.013
GAGGGGG403.2050593E-834.05
GAGGGCA200.002177709734.05
GAGGGAT200.002177709734.05
AGATCCT251.3950845E-434.014
GGGCGAG2100.032.380951
GGCGAGG2050.032.3414652
GCGAGGG2000.032.33
CGAGGGT552.9649527E-1030.909094
CGAGGGA401.5275637E-629.754
GTCACAG352.5191075E-529.1428579
GGTCACA352.5191075E-529.1428578
TCACAGA352.5191075E-529.14285710
ATCCTCT304.077294E-428.33333216
CCTCTTC304.077294E-428.33333218
GATCCTC304.077294E-428.33333215
Produced by FastQC (version 0.11.5)