##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526031_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2049 Sequences flagged as poor quality 0 Sequence length 40 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.809663250366032 4.0 4.0 30.0 4.0 33.0 2 9.92728160078087 4.0 4.0 16.0 4.0 28.0 3 9.554416788677404 4.0 4.0 13.0 4.0 28.0 4 9.111761835041484 4.0 4.0 10.0 4.0 26.0 5 8.974133723767691 4.0 4.0 9.0 4.0 27.0 6 7.717423133235725 4.0 4.0 4.0 4.0 21.0 7 8.798438262567107 4.0 4.0 4.0 4.0 27.0 8 8.837481698389459 4.0 4.0 4.0 4.0 27.0 9 8.851146900927281 4.0 4.0 4.0 4.0 27.0 10 8.069302098584675 4.0 4.0 4.0 4.0 24.0 11 8.530990727183992 4.0 4.0 4.0 4.0 27.0 12 8.40117130307467 4.0 4.0 4.0 4.0 28.0 13 8.282088823816496 4.0 4.0 4.0 4.0 26.0 14 8.054660810151294 4.0 4.0 4.0 4.0 26.0 15 8.300634455832114 4.0 4.0 4.0 4.0 28.0 16 7.091264031234749 4.0 4.0 4.0 4.0 21.0 17 6.921425085407516 4.0 4.0 4.0 4.0 19.0 18 6.903367496339678 4.0 4.0 4.0 4.0 19.0 19 6.715470961444607 4.0 4.0 4.0 4.0 18.0 20 6.673987310883358 4.0 4.0 4.0 4.0 18.0 21 6.559785261102977 4.0 4.0 4.0 4.0 16.0 22 6.410444119082479 4.0 4.0 4.0 4.0 15.0 23 6.402635431918009 4.0 4.0 4.0 4.0 15.0 24 6.141044411908248 4.0 4.0 4.0 4.0 11.0 25 6.345534407027818 4.0 4.0 4.0 4.0 15.0 26 6.40946803318692 4.0 4.0 4.0 4.0 15.0 27 6.259638848218644 4.0 4.0 4.0 4.0 11.0 28 6.499267935578331 4.0 4.0 4.0 4.0 15.0 29 6.033186920448999 4.0 4.0 4.0 4.0 10.0 30 6.308931185944363 4.0 4.0 4.0 4.0 13.0 31 6.682284040995608 4.0 4.0 4.0 4.0 17.0 32 6.526110297706198 4.0 4.0 4.0 4.0 15.0 33 6.582723279648609 4.0 4.0 4.0 4.0 16.0 34 6.509028794533919 4.0 4.0 4.0 4.0 15.0 35 6.468521229868228 4.0 4.0 4.0 4.0 11.0 36 6.435334309419229 4.0 4.0 4.0 4.0 4.0 37 6.340165934602245 4.0 4.0 4.0 4.0 4.0 38 6.296730112249878 4.0 4.0 4.0 4.0 4.0 39 6.285993167398731 4.0 4.0 4.0 4.0 4.0 40 6.387506100536847 4.0 4.0 4.0 4.0 4.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 17.74567099567099 1 2 22.38787878787879 1 3 -4.787445887445887 1 4 -4.9876623376623375 1 5 -4.204545454545457 1 6 -3.296536796536797 1 7 -4.79004329004329 1 8 -4.795887445887445 1 9 -5.138528138528137 1 10 -3.8794372294372295 1 11 -4.800649350649353 1 12 -4.312337662337663 1 13 -4.258658008658008 1 14 -4.212770562770563 1 15 -4.869047619047619 1 16 -3.972077922077922 1 17 -3.666666666666667 1 18 -3.4640692640692645 1 19 -2.9874458874458867 1 20 -3.395021645021645 1 21 -2.919696969696969 1 22 -2.288311688311688 1 23 -2.492424242424243 1 24 -2.642207792207792 1 25 -2.513203463203463 1 26 -2.698701298701298 1 27 -2.714935064935066 1 28 -3.0560606060606057 1 29 -2.2259740259740255 1 30 -2.6326839826839823 1 31 -3.0090909090909097 1 32 -2.622727272727273 1 33 -2.8110389610389612 1 34 -3.175541125541125 1 35 -2.642207792207792 1 36 -2.868181818181818 1 37 -2.545454545454546 1 38 -2.551298701298702 1 39 -2.280303030303031 1 40 -2.7826839826839826 2 1 4.412337662337661 2 2 -4.612121212121211 2 3 -0.45411255411255347 2 4 -3.9876623376623375 2 5 -0.5378787878787898 2 6 0.7034632034632029 2 7 -2.4567099567099566 2 8 -3.7958874458874448 2 9 -4.47186147186147 2 10 0.12056277056277054 2 11 -2.800649350649353 2 12 -2.3123376623376632 2 13 1.7413419913419919 2 14 0.12056277056277054 2 15 -2.5357142857142856 2 16 2.027922077922078 2 17 -2.666666666666667 2 18 1.2025974025974016 2 19 4.345887445887447 2 20 -2.061688311688312 2 21 5.080303030303031 2 22 4.045021645021646 2 23 1.1742424242424239 2 24 5.024458874458874 2 25 6.486796536796537 2 26 5.301298701298702 2 27 0.9517316017316011 2 28 4.943939393939394 2 29 -0.8926406926406925 2 30 6.367316017316018 2 31 5.99090909090909 2 32 -0.622727272727273 2 33 3.5222943722943727 2 34 5.157792207792209 2 35 4.024458874458874 2 36 8.465151515151515 2 37 7.12121212121212 2 38 7.782034632034632 2 39 8.053030303030303 2 40 7.550649350649351 3 1 6.745670995670993 3 2 2.3878787878787886 3 3 4.212554112554113 3 4 8.012337662337663 3 5 9.795454545454543 3 6 12.703463203463203 3 7 5.20995670995671 3 8 10.204112554112555 3 9 14.861471861471863 3 10 4.1205627705627705 3 11 15.199350649350647 3 12 16.68766233766234 3 13 7.741341991341992 3 14 11.787229437229437 3 15 15.130952380952381 3 16 13.027922077922078 3 17 15.333333333333332 3 18 20.535930735930734 3 19 11.012554112554113 3 20 21.604978354978357 3 21 9.080303030303032 3 22 12.711688311688313 3 23 6.507575757575757 3 24 13.357792207792208 3 25 16.486796536796536 3 26 20.301298701298702 3 27 22.285064935064934 3 28 21.943939393939395 3 29 10.774025974025975 3 30 17.367316017316018 3 31 25.990909090909092 3 32 17.377272727272725 3 33 17.18896103896104 3 34 25.824458874458877 3 35 16.357792207792208 3 36 21.131818181818183 3 37 16.454545454545453 3 38 25.448701298701298 3 39 15.719696969696969 3 40 24.217316017316016 5 1 -11.254329004329007 5 2 -6.612121212121211 5 3 -4.787445887445887 5 4 -4.9876623376623375 5 5 -4.204545454545457 5 6 -3.296536796536797 5 7 -4.79004329004329 5 8 -4.795887445887445 5 9 -5.138528138528137 5 10 -3.8794372294372295 5 11 -4.800649350649353 5 12 -4.312337662337663 5 13 -4.258658008658008 5 14 -4.212770562770563 5 15 -4.869047619047619 5 16 -3.972077922077922 5 17 -3.666666666666667 5 18 -3.4640692640692645 5 19 -2.9874458874458867 5 20 -3.395021645021645 5 21 -2.919696969696969 5 22 -2.288311688311688 5 23 -2.492424242424243 5 24 -2.642207792207792 5 25 -2.513203463203463 5 26 -2.698701298701298 5 27 -2.714935064935066 5 28 -3.0560606060606057 5 29 -2.2259740259740255 5 30 -2.6326839826839823 5 31 -3.0090909090909097 5 32 -2.622727272727273 5 33 -2.8110389610389612 5 34 -3.175541125541125 5 35 -2.642207792207792 5 36 -2.868181818181818 5 37 -2.545454545454546 5 38 -2.551298701298702 5 39 -2.280303030303031 5 40 -2.7826839826839826 6 1 -11.254329004329007 6 2 -6.612121212121211 6 3 -4.787445887445887 6 4 -4.9876623376623375 6 5 -4.204545454545457 6 6 -3.296536796536797 6 7 -4.79004329004329 6 8 -4.795887445887445 6 9 -5.138528138528137 6 10 -3.8794372294372295 6 11 -4.800649350649353 6 12 -4.312337662337663 6 13 -4.258658008658008 6 14 -4.212770562770563 6 15 -4.869047619047619 6 16 -3.972077922077922 6 17 -3.666666666666667 6 18 -3.4640692640692645 6 19 -2.9874458874458867 6 20 -3.395021645021645 6 21 -2.919696969696969 6 22 -2.288311688311688 6 23 -2.492424242424243 6 24 -2.642207792207792 6 25 -2.513203463203463 6 26 -2.698701298701298 6 27 -2.714935064935066 6 28 -3.0560606060606057 6 29 -2.2259740259740255 6 30 -2.6326839826839823 6 31 -3.0090909090909097 6 32 -2.622727272727273 6 33 -2.8110389610389612 6 34 -3.175541125541125 6 35 -2.642207792207792 6 36 -2.868181818181818 6 37 -2.545454545454546 6 38 -2.551298701298702 6 39 -2.280303030303031 6 40 -2.7826839826839826 8 1 -2.5876623376623407 8 2 -1.6121212121212114 8 3 -1.1207792207792204 8 4 -0.9876623376623375 8 5 2.128787878787877 8 6 -0.9632034632034641 8 7 4.876623376623376 8 8 4.870779220779221 8 9 2.8614718614718626 8 10 3.4538961038961045 8 11 3.199350649350647 8 12 1.3543290043290028 8 13 -2.258658008658008 8 14 -2.5461038961038964 8 15 1.1309523809523814 8 16 -0.6387445887445891 8 17 2.666666666666667 8 18 0.20259740259740244 8 19 -0.9874458874458867 8 20 4.604978354978355 8 21 6.746969696969697 8 22 7.378354978354978 8 23 4.840909090909091 8 24 2.0244588744588743 8 25 2.486796536796537 8 26 4.967965367965368 8 27 4.618398268398268 8 28 3.9439393939393943 8 29 4.7740259740259745 8 30 6.367316017316018 8 31 6.324242424242424 8 32 6.710606060606061 8 33 1.8556277056277048 8 34 5.491125541125541 8 35 6.691125541125542 8 36 6.465151515151516 8 37 3.787878787878788 8 38 3.782034632034632 8 39 6.719696969696969 8 40 5.217316017316017 9 1 -1.5876623376623407 9 2 -1.6121212121212114 9 3 2.8792207792207787 9 4 3.6790043290043286 9 5 -1.8712121212121238 9 6 -1.296536796536797 9 7 3.2099567099567103 9 8 -2.4625541125541117 9 9 -3.1385281385281374 9 10 -1.5461038961038964 9 11 4.199350649350647 9 12 -1.3123376623376632 9 13 3.741341991341992 9 14 -2.5461038961038964 9 15 0.7976190476190474 9 16 -2.305411255411255 9 17 -2.666666666666667 9 18 5.2025974025974016 9 19 1.0125541125541133 9 20 -1.3950216450216448 9 21 6.746969696969697 9 22 8.045021645021645 9 23 2.174242424242423 9 24 5.357792207792208 9 25 2.153463203463203 9 26 -1.0320346320346312 9 27 -0.048268398268398904 9 28 -1.0560606060606057 9 29 1.1073593073593075 9 30 2.033982683982684 9 31 3.324242424242424 9 32 4.710606060606061 9 33 6.855627705627705 9 34 4.157792207792209 9 35 5.357792207792208 9 36 3.131818181818182 9 37 6.454545454545454 9 38 4.448701298701298 9 39 8.053030303030303 9 40 7.550649350649351 10 1 13.745670995670993 10 2 2.3878787878787886 10 3 3.2125541125541126 10 4 5.0123376623376625 10 5 1.7954545454545432 10 6 8.703463203463203 10 7 14.20995670995671 10 8 13.204112554112555 10 9 15.861471861471863 10 10 9.12056277056277 10 11 20.19935064935065 10 12 25.68766233766234 10 13 10.741341991341992 10 14 18.787229437229435 10 15 25.13095238095238 10 16 20.027922077922078 10 17 11.333333333333332 10 18 11.535930735930735 10 19 3.0125541125541133 10 20 23.604978354978357 10 21 26.080303030303032 10 22 17.711688311688313 10 23 20.507575757575758 10 24 20.357792207792208 10 25 16.486796536796536 10 26 9.301298701298702 10 27 11.285064935064934 10 28 23.943939393939395 10 29 24.774025974025975 10 30 -2.6326839826839823 10 31 -3.0090909090909097 10 32 -2.622727272727273 10 33 -2.8110389610389612 10 34 -3.175541125541125 10 35 -2.642207792207792 10 36 -2.868181818181818 10 37 -2.545454545454546 10 38 -2.551298701298702 10 39 -2.280303030303031 10 40 -2.7826839826839826 16 1 15.245670995670993 16 2 -2.6121212121212114 16 3 0.7125541125541126 16 4 -3.9876623376623375 16 5 -3.204545454545457 16 6 3.203463203463203 16 7 -3.2900432900432897 16 8 1.2041125541125552 16 9 -0.8885281385281374 16 10 -0.37943722943722946 16 11 1.199350649350647 16 12 -2.8123376623376632 16 13 -2.008658008658008 16 14 1.7872294372294366 16 15 -2.1190476190476186 16 16 3.277922077922078 16 17 3.583333333333333 16 18 3.7859307359307355 16 19 2.7625541125541133 16 20 2.354978354978355 16 21 -0.919696969696969 16 22 4.211688311688312 16 23 2.757575757575757 16 24 -1.1422077922077918 16 25 3.986796536796537 16 26 0.5512987012987018 16 27 4.035064935064934 16 28 3.6939393939393943 16 29 4.7740259740259745 16 30 4.617316017316018 16 31 -1.0090909090909097 16 32 3.877272727272727 16 33 2.6889610389610388 16 34 4.074458874458875 16 35 -2.642207792207792 16 36 -2.868181818181818 16 37 -2.545454545454546 16 38 -2.551298701298702 16 39 -2.280303030303031 16 40 -2.7826839826839826 18 1 20.24567099567099 18 2 18.88787878787879 18 3 16.712554112554113 18 4 26.262337662337664 18 5 27.295454545454543 18 6 26.4534632034632 18 7 23.20995670995671 18 8 24.704112554112555 18 9 25.361471861471863 18 10 17.87056277056277 18 11 21.19935064935065 18 12 25.43766233766234 18 13 29.24134199134199 18 14 25.037229437229435 18 15 24.38095238095238 18 16 14.777922077922078 18 17 15.083333333333332 18 18 7.2859307359307355 18 19 15.762554112554113 18 20 14.604978354978355 18 21 15.580303030303032 18 22 8.461688311688313 18 23 14.507575757575758 18 24 17.357792207792208 18 25 13.986796536796536 18 26 15.551298701298702 18 27 15.035064935064934 18 28 16.193939393939395 18 29 16.274025974025975 18 30 16.617316017316018 18 31 20.740909090909092 18 32 22.127272727272725 18 33 20.93896103896104 18 34 19.574458874458877 18 35 19.357792207792208 18 36 18.131818181818183 18 37 9.704545454545453 18 38 18.198701298701298 18 39 10.219696969696969 18 40 16.217316017316016 19 1 16.74567099567099 19 2 11.787878787878787 19 3 9.212554112554113 19 4 16.412337662337663 19 5 20.595454545454544 19 6 15.503463203463204 19 7 21.80995670995671 19 8 23.004112554112556 19 9 23.26147186147186 19 10 19.720562770562772 19 11 13.999350649350648 19 12 16.887662337662334 19 13 18.74134199134199 19 14 19.787229437229435 19 15 20.73095238095238 19 16 19.62792207792208 19 17 20.333333333333332 19 18 16.735930735930737 19 19 16.212554112554113 19 20 19.604978354978357 19 21 11.48030303030303 19 22 10.911688311688312 19 23 9.507575757575758 19 24 11.557792207792208 19 25 4.086796536796537 19 26 7.1012987012987026 19 27 11.085064935064935 19 28 8.343939393939394 19 29 11.174025974025973 19 30 11.167316017316018 19 31 16.190909090909088 19 32 14.577272727272726 19 33 12.388961038961039 19 34 14.424458874458876 19 35 14.557792207792208 19 36 9.731818181818184 19 37 12.454545454545453 19 38 12.648701298701297 19 39 12.719696969696969 19 40 11.817316017316019 20 1 -11.254329004329007 20 2 -6.612121212121211 20 3 -4.787445887445887 20 4 -4.9876623376623375 20 5 -4.204545454545457 20 6 -3.296536796536797 20 7 -4.79004329004329 20 8 -4.795887445887445 20 9 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13.361471861471863 26 10 14.62056277056277 26 11 12.699350649350647 26 12 2.1876623376623368 26 13 16.24134199134199 26 14 20.787229437229435 26 15 10.630952380952381 26 16 21.027922077922078 26 17 22.833333333333332 26 18 10.535930735930735 26 19 20.012554112554113 26 20 13.104978354978355 26 21 8.080303030303032 26 22 5.711688311688312 26 23 -0.9924242424242431 26 24 4.357792207792208 26 25 6.486796536796537 26 26 11.301298701298702 26 27 11.785064935064934 26 28 10.943939393939395 26 29 5.2740259740259745 26 30 9.867316017316018 26 31 12.49090909090909 26 32 13.377272727272727 26 33 13.18896103896104 26 34 12.824458874458875 26 35 13.357792207792208 26 36 11.631818181818183 26 37 11.954545454545453 26 38 11.948701298701298 26 39 8.219696969696969 26 40 12.717316017316017 28 1 -11.254329004329007 28 2 -6.612121212121211 28 3 -4.787445887445887 28 4 -4.9876623376623375 28 5 -4.204545454545457 28 6 -3.296536796536797 28 7 -4.79004329004329 28 8 -4.795887445887445 28 9 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-2.6326839826839823 118 31 -3.0090909090909097 118 32 -2.622727272727273 118 33 -2.8110389610389612 118 34 -3.175541125541125 118 35 -2.642207792207792 118 36 -2.868181818181818 118 37 -2.545454545454546 118 38 -2.551298701298702 118 39 -2.280303030303031 118 40 -2.7826839826839826 119 1 8.745670995670993 119 2 9.387878787878789 119 3 13.212554112554113 119 4 12.012337662337663 119 5 12.462121212121211 119 6 7.370129870129869 119 7 5.543290043290044 119 8 12.204112554112555 119 9 12.861471861471863 119 10 7.1205627705627705 119 11 4.199350649350647 119 12 6.687662337662337 119 13 0.7413419913419919 119 14 1.1205627705627705 119 15 -0.20238095238095255 119 16 -1.3054112554112551 119 17 -1.0 119 18 -0.7974025974025976 119 19 6.012554112554113 119 20 5.938311688311689 119 21 -2.919696969696969 119 22 -2.288311688311688 119 23 -2.492424242424243 119 24 -2.642207792207792 119 25 -2.513203463203463 119 26 -2.698701298701298 119 27 -2.714935064935066 119 28 -3.0560606060606057 119 29 -2.2259740259740255 119 30 -2.6326839826839823 119 31 -3.0090909090909097 119 32 -2.622727272727273 119 33 -2.8110389610389612 119 34 -3.175541125541125 119 35 -2.642207792207792 119 36 -2.868181818181818 119 37 -2.545454545454546 119 38 -2.551298701298702 119 39 -2.280303030303031 119 40 -2.7826839826839826 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 1430.0 5 52.0 6 39.0 7 38.0 8 27.0 9 31.0 10 40.0 11 35.0 12 35.0 13 24.0 14 21.0 15 19.0 16 14.0 17 11.0 18 10.0 19 16.0 20 33.0 21 33.0 22 29.0 23 26.0 24 18.0 25 13.0 26 13.0 27 11.0 28 16.0 29 8.0 30 6.0 31 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.12066438690766 0.48851978505129456 0.14655593551538837 0.24425989252564728 8 98.04782820888238 0.9760858955588092 0.634455832113226 0.3416300634455832 9 97.359413202934 0.7334963325183375 1.5158924205378974 0.3911980440097799 10 37.774524158125914 29.575402635431917 21.18106393362616 11.469009272816008 11 26.598340653977548 23.37725719863348 23.133235724743777 26.891166422645192 12 26.79355783308931 29.6730112249878 22.889214250854074 20.644216691068813 13 21.2298682284041 22.986822840409957 24.841386041971695 30.94192288921425 14 20.263671875 38.330078125 23.14453125 18.26171875 15 39.33626159102001 23.23084431429966 24.54856027330405 12.88433382137628 16 27.33040507564666 37.042459736456806 21.717911176183506 13.90922401171303 17 23.32845290385554 22.64519277696437 38.84821864324061 15.178135675939483 18 21.425085407515862 25.085407515861398 24.987798926305516 28.501708150317228 19 22.547584187408493 24.30453879941435 24.987798926305516 28.160078086871643 20 21.522693997071745 23.865300146412885 38.604197169350904 16.00780868716447 21 21.571498291849682 21.815519765739385 28.111273792093705 28.501708150317228 22 16.886285993167398 21.669106881405565 41.92288921425085 19.521717911176182 23 15.617374328940947 23.816495851634944 40.6539775500244 19.91215226939971 24 18.594436310395317 27.086383601756953 26.403123474865787 27.916056612981944 25 22.47191011235955 26.819736199316075 35.12457254518808 15.583781143136296 26 35.47280744732974 27.731504164625182 20.333170014698677 16.4625183733464 27 17.091087169441725 39.42213516160627 23.75122428991185 19.73555337904016 28 24.91450903761602 35.515388373229115 22.61846604787494 16.95163654127992 29 30.965213130818224 29.691327780499755 22.53797158255757 16.805487506124447 30 26.171875 25.87890625 32.6171875 15.33203125 31 27.81844802342606 32.99170326988775 24.69497315763787 14.494875549048317 32 27.93542074363992 29.207436399217222 25.782778864970645 17.074363992172213 33 26.159102000976088 32.30844314299659 24.45095168374817 17.08150317227916 34 18.936066373840898 27.33040507564666 39.23865300146413 14.494875549048317 35 16.642264519277695 27.281600780868715 40.55636896046852 15.519765739385067 36 23.655913978494624 27.174975562072333 30.98729227761486 18.181818181818183 37 27.037579306979016 26.110297706198143 30.01464128843338 16.83748169838946 38 27.76964372864812 25.8174719375305 28.94094680331869 17.471937530502686 39 23.57247437774524 28.452903855539287 24.646168862859934 23.32845290385554 40 23.523670082967303 26.159102000976088 34.94387506100537 15.373352855051245 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 2.0 20 4.0 21 11.0 22 18.0 23 18.0 24 14.0 25 10.0 26 8.5 27 7.0 28 7.0 29 13.0 30 19.0 31 23.0 32 27.0 33 27.0 34 31.0 35 35.0 36 34.5 37 34.0 38 34.0 39 47.0 40 60.0 41 75.0 42 90.0 43 90.0 44 102.5 45 115.0 46 145.0 47 175.0 48 175.0 49 195.0 50 215.0 51 209.5 52 204.0 53 204.0 54 190.0 55 176.0 56 179.5 57 183.0 58 183.0 59 188.0 60 193.0 61 177.5 62 162.0 63 162.0 64 145.0 65 128.0 66 111.5 67 95.0 68 95.0 69 75.5 70 56.0 71 38.0 72 20.0 73 20.0 74 13.5 75 7.0 76 7.0 77 7.0 78 7.0 79 5.5 80 4.0 81 3.0 82 2.0 83 2.0 84 2.0 85 2.0 86 1.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.09760858955588092 8 0.0 9 0.19521717911176184 10 0.0 11 0.0 12 0.0 13 0.0 14 0.04880429477794046 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.09760858955588092 26 0.3904343582235237 27 0.3416300634455832 28 0.09760858955588092 29 0.3904343582235237 30 0.04880429477794046 31 0.0 32 0.2440214738897023 33 0.0 34 0.0 35 0.0 36 0.14641288433382138 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 2049.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.55490483162518 #Duplication Level Percentage of deduplicated Percentage of total 1 94.3701226309922 82.62567105905319 2 4.069119286510591 7.125427037579307 3 0.5574136008918618 1.4641288433382138 4 0.39018952062430323 1.3665202537823329 5 0.16722408026755853 0.7320644216691069 6 0.055741360089186176 0.29282576866764276 7 0.11148272017837235 0.6832601268911664 8 0.11148272017837235 0.7808687164470474 9 0.0 0.0 >10 0.16722408026755853 4.929233772571987 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGCGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 44 2.1473889702293802 No Hit GGGCGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 38 1.8545632015617375 No Hit GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 19 0.9272816007808687 No Hit GGGCGAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG 8 0.3904343582235237 No Hit GGGCGAGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG 8 0.3904343582235237 No Hit GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 7 0.3416300634455832 No Hit GGGCGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.3416300634455832 No Hit GGGCGAGGGTCCCAGATCCTCTTCTGAAGGAGATTGGGCG 6 0.29282576866764276 No Hit GGGCGAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 5 0.2440214738897023 No Hit GGGCGAGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT 5 0.2440214738897023 No Hit GGGCGAGGGGACGCTACTCAGACTACCCAGATGCTTACAC 5 0.2440214738897023 No Hit GGGCGAGGGTCACAGATCCTCTTCTGAGATGAGGATCGGA 4 0.19521717911176184 No Hit GGGCGAGGGTCACAGCTCCTCTTCTGAAGGAGATTGGGCG 4 0.19521717911176184 No Hit GGGCGAGGGGCTGGGCGAGGGTCACAGATCCTCTTCTGAG 4 0.19521717911176184 No Hit GGGCGAGGGATTCCAAAGCTTTCCAGCATAATTTCTGGCT 4 0.19521717911176184 No Hit GGGCGAGGGGGAGGAAGAAGGAGAGGAATACTAAATTAAA 4 0.19521717911176184 No Hit GGGCGAGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT 4 0.19521717911176184 No Hit GGGCGAGGGTCACAGATACTCTTCTGAAGGAGATTGGGCG 4 0.19521717911176184 No Hit GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 3 0.14641288433382138 No Hit GGGCGAGGTCACAGATCCTCTTCTGAGATGAGGATCGGAA 3 0.14641288433382138 No Hit GGGCGAGGGTCACAGCTCCTCTTCTGAGATGAGTTTTTGT 3 0.14641288433382138 No Hit GGGCGAGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT 3 0.14641288433382138 No Hit GGGCGAGGGTGCTGCGTCCTTATGAATGTTTTGACCATTT 3 0.14641288433382138 No Hit GGGCGAGGGATGCCAAAGTGTTCTTAGTGGCTGATACTGG 3 0.14641288433382138 No Hit GGGCGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.14641288433382138 No Hit GGGCGAGGGAGCTTTTAACCATTGTAGGCCTAAAAGCAGC 3 0.14641288433382138 No Hit GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT 3 0.14641288433382138 No Hit GGGCGAGGGATCAATAAATGAAAACTGTTCAAAAATTTTA 3 0.14641288433382138 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.09760858955588092 0.0 0.0 0.0 0.0 15 0.09760858955588092 0.0 0.0 0.0 0.0 16 0.09760858955588092 0.0 0.0 0.0 0.0 17 0.09760858955588092 0.0 0.0 0.0 0.0 18 0.09760858955588092 0.0 0.0 0.0 0.0 19 0.09760858955588092 0.0 0.0 0.0 0.0 20 0.09760858955588092 0.0 0.0 0.0 0.0 21 0.09760858955588092 0.0 0.0 0.0 0.0 22 0.09760858955588092 0.0 0.0 0.0 0.0 23 0.09760858955588092 0.0 0.0 0.0 0.0 24 0.09760858955588092 0.0 0.0 0.0 0.0 25 0.09760858955588092 0.0 0.0 0.0 0.0 26 0.14641288433382138 0.0 0.0 0.0 0.0 27 0.14641288433382138 0.0 0.0 0.0 0.0 28 0.14641288433382138 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGGGG 25 1.3950845E-4 34.0 6 ACAGATC 20 0.0021777097 34.0 12 CGAGGGG 75 0.0 34.0 4 CGAGGGC 30 8.746991E-6 34.0 4 CAGATCC 25 1.3950845E-4 34.0 13 GAGGGGG 40 3.2050593E-8 34.0 5 GAGGGCA 20 0.0021777097 34.0 5 GAGGGAT 20 0.0021777097 34.0 5 AGATCCT 25 1.3950845E-4 34.0 14 GGGCGAG 210 0.0 32.38095 1 GGCGAGG 205 0.0 32.341465 2 GCGAGGG 200 0.0 32.3 3 CGAGGGT 55 2.9649527E-10 30.90909 4 CGAGGGA 40 1.5275637E-6 29.75 4 GTCACAG 35 2.5191075E-5 29.142857 9 GGTCACA 35 2.5191075E-5 29.142857 8 TCACAGA 35 2.5191075E-5 29.142857 10 ATCCTCT 30 4.077294E-4 28.333332 16 CCTCTTC 30 4.077294E-4 28.333332 18 GATCCTC 30 4.077294E-4 28.333332 15 >>END_MODULE